C:\
dmautop\
temp\
DCTM_
ARP.
doc
Marine
Flight
Station
Procedures
(
using
MF­
25
CTD)

These
instructions
assume
that
the
CTD
and
AFM
are
already
programmed.

Set
Niskin
bottles
 
use
AFM
arm
positions
3,
9,
11
and
12.
Ensure
vents
&
spigots
are
closed
&
turned
inside
to
prevent
damage.
Turn
on
CTD
Lower
CTD
 
Hold
just
below
surface
for
at
least
90
seconds.
Take
fecal
sample

Open
fecal
bottle
and
place
in
bottle
holder

Lower
just
below
surface
and
fill
bottle
(
sample
should
be
from
the
surface
micro­
layer)


Recover
bottle;
pour
out
small
amount
to
fit
cork;
stopper
bottle
and
place
on
ice.
Raise
CTD
to
first
white
plastic
divider
of
frame.
Hold
for
6
seconds,
then
lower
CTD
to1­
2
meters
above
bottom.
(
When
CTD
reaches
bottom
and
line
goes
slack,
raise
so
CTD
does
not
drag
on
bottom.)
Hold
for
60
seconds.
Take
secchi
reading
to
closest
half
meter.
Each
line
marking
is
equal
to
0.5
m.
Raise
CTD
until
it
just
breaks
the
surface,
hold
until
surface
bottle
fires,
then
bring
on
board.
Turn
CTD
off
and
check
bottles
for
leaking.
Take
DO
sample
and
preserve.


Attach
tubing
to
spigot
and
remove
air
bubbles.


Rinse
DO
bottle
by
placing
tubing
inside
against
bottom,
inverting
bottle,
then
rotating
bottle
3x
with
water
flowing
down
sides.


Gently
invert
bottle
allowing
water
to
overflow
for
approx.
3
volumes.


Shut
off
Niskin
valve
and
gently
remove
tubing,
without
introducing
any
air
into
the
sample.


Add
1
mL
MnCl2.

Add
1
mL
NaOH­
NaI
Azide.


Insert
stopper
at
an
angle
to
prevent
air
from
being
trapped
by
stopper.
Twist
tightly
&
quickly
invert
bottle
to
mix
solutions.


Place
sample
in
DO
box.
Take
salinity
sample
(
if
needed).


Rinse
bottle
three
times.


Fill
to
approximately
2/
3
full.
Take
nutrient
samples.


Attach
new
filter
to
syringe.

Rinse
syringe
3
times
with
water
from
Niskin
bottle.


Fill
syringe
to
top
and
insert
plunger.


Rinse
sample
bottle
and
cap
3
times
with
water
from
syringe
(
leaving
40
ml
in
syringe).


Filter
remaining
40
ml
into
sample
bottle,
then
cap
and
place
on
ice.
Take
chl­
a
samples.


Rinse
bottle
and
cap
3
times
with
water
from
Niskin.


Fill
bottle
to
overflowing
(
positive
meniscus)
and
cap.

Place
on
ice.
C:\
dmautop\
temp\
DCTM_
ARP.
doc
Take
Monica's
samples
on
MF4
stations
HCB006,
HCB004
and
HCB003
and
on
MF3
at
ADM002,
at
the
following
depths.
(
If
water
or
time
constraints
exist,
priority
should
be
given
to
surface
samples
with
the
deepest
samples
dropped
first.)

HCB006
0,
10,
30

HCB004
0,
10,
30

HCB003
0

ADM002
0,
10,
30

Rinse
bottle
and
cap
3
times.


Fill
sample
bottle
to
overflowing.


Store
on
ice
in
the
extra,
small
cooler.


Leave
samples
on
plane
at
end
of
flight,
as
Monica
will
pick
up
samples
at
Kenmore
Air;
call
her
to
inform
her
of
ETA
of
plane
at
Kenmore.
Drain
and
reset
Niskin
bottles.
Close
hatch
and
secure
CTD.
Proceed
to
next
station.
Attach
computer
cable
to
CTD.
Download
data
and
reprogram
CTD/
AFM
en
route
to
next
station.
Wait
2
minutes
before
next
cast,
if
this
amount
of
time
has
not
passed
between
downloading
CTD
&
arrival
at
new
station.
Transfer
all
CTD
casts
to
floppy
disk
on
return
flight
to
dock.
