Page
1
of
23
pages
Table
of
Contents
This
draft
master
general
permit
package
contains
the
items
listed
below:

1.
Table
of
Contents
2.
Fact
Sheet
/
Statement
of
Basis
3.
Part
I
 
General
Requirements:
NJPDES
4.
Part
II
 
General
Requirements:
Discharge
Categories
5.
Part
III
 
Limits
and
Monitoring
Requirements
6.
Attachments
­
Table
B:
List
of
Waterbodies
Eligible
Under
Table
B
Table
D:
Remediation
Standards
for
compounds
that
can
be
authorized
for
discharge
under
Table
D.
MTBE
Reporting
Guidance
7.
Part
IV
 
Specific
Requirements:
Narrative
8.
Appendix
A
(
Included
if
chronic
monitoring/
limit
requirements
are
applied
under
Table
D)
Part
III­
Attachment
B
Page
2
of
23
pages
GPPC
Master
Permit
Renewal
Waterbodies
Eligible
for
Discharge
Under
Table
B
of
the
GPPC
Renewal
Permit*

Any
existing
or
proposed
discharges
to
the
waterbodies
specified
below,
or
to
waterbodies
upstream
of
the
waterbodies
specified
below,
are
ineligible
for
discharge
under
Table
A,
but
may
be
eligible
for
discharge
under
Table
B.

Note:
Counties
listed
indicate
the
primary
locations
of
the
listed
waterbodies;
however,
these
waterbodies
may
flow
from
or
into
other
bordering
counties.
Alternate
or
local
spellings
are
included
in
"(
)".
Any
discharges
to
portions
of
these
waterbodies
which
are
classified
as
FW1,
C1
or
PL
waters
are
ineligible
for
authorization
under
Tables
A
or
B.

Atlantic
County
Mercer
County
Absecon
Creek
and
its
unnamed
tributaries
Millstone
River
and
its
unnamed
tribs.
Jarretts
Run
Delaware­
Raritan
Canal
Doughty
Pond
North
Branch
(
Absecon
Creek)
South
Branch
(
Absecon
Creek)

Bergen
County
Spark
Hill
(
Sparkill)
Creek
Hirshfeld
Brook
and
its
unnamed
tributaries
Oradell
Reservoir
Saddle
River
and
its
unnamed
tributaries
Sprout
Brook
and
its
unnamed
tributaries
Pehle
Brook
Middlesex
County
Jordan
Brook
Millstone
River
and
its
unnamed
tributaries
Hohokus
Brook
and
its
unnamed
tributaries
Heathcote
Brook
and
its
unnamed
tributaries
Allendale
Brook
Carters
Brook
and
its
unnamed
tributaries
Ramsey
Brook
Heathcote
Brook
Branch
Valentine
Brook
Harrys
Brook
Saddle
Brook
Lawrence
Brook
and
its
unnamed
tribs.
Pine
Brook
Sawmill
Brook
and
its
unnamed
tribs.
Haledon
Reservoir
Sucker
Brook
Long
Swamp
Brook
Beaver
Dam
Brook
Burd
Reservoir
Ireland
Brook
Oakeys
Brook
and
its
unnamed
tribs.
Essex
County
Cow
Yard
Brook
Canoe
Brook
Terhune
Run
Canoe
Brook
Reservoir
Tennents
Pond
Taylor
Brook
Tennents
Brook
(
Creek)
and
its
unnamed
tribs.
Bear
Brook
Warnes
Brook
Cub
Brook
and
its
unnamed
tributaries
Matchaponix
Brook
and
its
unnamed
tribs.
Orange
Reservoir
Barclay
Brook
and
its
unnamed
tributaries
East
Branch
Rahway
River
West
Branch
Rahway
River
and
its
tribs.
Turtle
Brook
Hunterdon
County
Swan
Creek
Reservoir
East
Swan
Creek
Reservoir
West
Delaware­
Raritan
Canal
Part
III­
Attachment
B
Page
3
of
23
pages
GPPC
Master
Permit
Renewal
Waterbodies
Eligible
for
Discharge
Under
Table
B
of
the
GPPC
Renewal
Permit*
(
contd.)

Morris
County
Monmouth
County
Taylor
Town
Reservoir
Swimming
River
and
its
unnamed
tribs.
Mine
Brook
and
its
tributaries
Swimming
River
Reservoir
Lower
Mine
Hill
Reservoir
Pine
Brook
and
its
unnamed
tributaries
Clyde
Potts
Reservoir
Hop
Brook
and
its
unnamed
tributaries
Beaver
Creek
(
Brook)
Willow
Brook
and
its
unnamed
tributaries
Split
Rock
Reservoir
Big
Brook
and
its
unnamed
tributaries
Burd
Reservoir
Trout
Brook
Charlottesburg
Reservoir
Yellow
Brook
and
its
unnamed
tributaries
Lake
Kinnelon
Slope
Brook
Kakeout
Reservoir
Manasquan
River
and
its
unnamed
tributaries
Butler
Reservoir
Manasquan
Reservoir
Cooks
Creek
Ocean
County
Deep
Creek
Metdeconk
River
and
its
unnamed
tribs.
Crabtown
Creek
Cedar
Bridge
Brook
and
tribs.
Debbies
Creek
South
Branch
(
Metdeconk
River)
Watson
Creek
North
Branch
(
Metdeconk
River)
Judas
Creek
Cotteral
Brook
and
its
unnamed
tribs.
Roberts
Swamp
Brook
Cabinfield/
Schoolhouse
Brook
and
its
tribs.
Bayhead­
Manasquan
Canal
Gravelly
Run
Sawmill
Creek
Muddy
Ford
Brook
and
its
tributaries
Squankum
Brook
and
its
unnamed
tribs.
Snipe
Creek
and
its
tributaries
Mingamahone
Brook
and
its
tribs.
Turtle
Brook
and
its
tributaries
Long
Swamp
Brook
Ridge
Creek
E.
Branch
Mingamahone
Brook
and
its
tribs.
Watering
Place
Brook
and
its
tributaries
Bear
Swamp
Brook
Marsh
Bog
Brook
and
its
unnamed
tribs.
Passaic
County
Bannen
Meadow
Brook
Pequannock
River
and
its
unnamed
tribs.
Cricket
Creek
Oak
Ridge
Reservoir
Cattail
Brook
Wanaque
River
and
its
unnamed
tribs.
DeBois
Creek
and
its
unnamed
tribs.
Wanaque
Reservoir
Burkes
Creek
.
Stone
House
Brook
Applegates
Creek
Macopin
River
Shark
River
and
its
unnamed
tribs.
Timber
Brook
Musquash
Brook
Pacock
(
Pacack)
Brook
and
its
unnamed
tribs.
Laurel
Gully
Charlottesburg
Reservoir
Jumping
Brook
Point
View
Reservoir
Wills
Brook
Hankins
Brook
Salem
County
Robins
Swamp
Brook
Salem
Canal
Reevy
Branch
of
Shark
River
Laurel
Lake
Matchaponix
Brook
and
its
unnamed
tribs.
Elkinton
Mill
Pond
Pine
Brook
and
its
unnamed
tribs.
Rocky
Brook
and
its
unnamed
tribs.
Weamaconk
Creek
and
its
unnamed
tribs.
McGellairds
Brook
and
its
unnamed
tribs.
Part
III­
Attachment
B
Page
4
of
23
pages
GPPC
Master
Permit
Renewal
Waterbodies
Eligible
for
Discharge
Under
Table
B
of
the
GPPC
Renewal
Permit*
(
continued)

Somerset
County
Sussex
County
Delaware­
Raritan
Canal
Dry
Brook
Reservoir
Millstone
River
and
its
tributaries
Franklin
Pond
Royce
Brook
and
its
unnamed
tributaries
Franklin
Pond
Creek
and
its
tributaries
Six
Mile
Run
and
its
unnamed
tributaries
Morris
Lake
(
Newton
Reservoir)
Middlebush
Brook
and
its
unnamed
tributaries
Lake
Rutherford
Ten
Mile
Run
and
its
unnamed
tributaries
Simonson
Brook
and
its
unnamed
tributaries
Union
County
Bedens
Brook
and
its
unnamed
tributaries
Rahway
River
and
its
unnamed
tributaries
Pike
Run
and
its
unnamed
tributaries
upstream
of
Valley
Rd.
Rock
Brook
and
its
unnamed
tributaries
Nomaheagan
Brook
and
its
unnamed
tribs.
Turtle
Brook
Counties
of
Union,
Middlesex,
Monmouth,
Ocean,
Burlington,
Atlantic,
Cape
May,
Cumberland
and
Salem:
Shellfish
Harvesting
Waters
The
Department
reserves
the
right
to
deny
an
individual
authorization
under
the
GPPC
permit
renewal
to
any
proposed
discharges
to
waterbodies
identified
in
the
Department's
annual
Shellfish
Growing
Classification
Charts
under
the
following
shellfish
growing
water
classification
codes:
special
restricted
areas;
seasonal
areas;
prohibited
areas
and;
approved
areas.
The
designated
uses
for
waterbodies
classified
as
SE1
or
SC
include
shellfish
harvesting
in
accordance
with
N.
J.
A.
C.
7:
9B­
1.1
et
seq.
If
the
Department
determines
that
it
can
not
approve
a
discharge
to
a
waterbody
used
for
shellfish
harvesting
under
the
GPPC
permit
renewal,
the
applicant
will
be
notified
of
the
Department's
finding.
The
Department
will
also
notify
the
applicant
if
the
discharge
can
be
authorized
under
an
individual
NJPDES
DSW
permit.
If
the
Department
can
authorize
a
discharge
to
a
waterbody
classified
as
SE1
or
SC
under
the
GPPC
permit
renewal,
the
Department
would
issue
such
authorization
under
Table
B.

All
Counties
In
issuing
requests
for
authorization
under
the
GPPC
permit
renewal,
the
Department
may
deem
discharges
to
other
waterbodies
ineligible
under
Table
A
on
a
case­
by­
case
basis.
These
circumstances
may
apply
to
a
discharge
proposed
to
a
waterbody
where
this
waterbody
recharges
potable
groundwater
wells
downgradient
of
the
proposed
discharge.
Or,
information
may
become
available
to
the
Department
which
shows
that
a
waterbody
not
included
in
this
list
is
used
for
potable
water
purposes.
If
the
Department
determines
that
such
site­
specific
circumstances
apply
and
the
conditions
of
Table
A
are
not
appropriate,
the
Department
will
notify
the
applicant
prior
to
issuing
an
individual
GPPC
authorization
under
Table
B.
Part
III
­
Attachment
D
Page
5
of
23
pages
GPPC
Master
permit
Renewal
Other
Pollutants
that
may
be
Limited
Under
Table
D
In
addition
to
complying
with
the
effluent
limitations
and
monitoring
conditions
of
Table
D
on
the
preceding
pages,
any
other
parameters
indicated
below
may
also
be
limited
in
an
individual
authorization.
These
additional
parameters
will
be
included
in
Part
III
of
the
individual
authorization.
All
units
are
in
µ
g/
L.
FW2
Waters
SE,
SC
Waters
Parameter
Monthly
/
Daily
Monthly
/
Daily
Avg.
/
Maximum
Avg.
/
Maximum
RQL*
Volatile
Compounds
Acrolein
NL
100
NL
100
50
Acrylonitrile
NL
100
NL
100
50
Bromoform
NL
8.6
29
58
8
Carbon
Tetrachloride
NL
6
8.8
NL
6
Chlorobenzene
15
28
15
28
6
Chlorodibromomethane
NL
8.2
NL
14
6
Chlorethane
104
268
104
268
­
Chloroform
NL
11.4
21
46
5
Dichlorobromomethane
NL
5
NL
12
5
1,1­
Dichloroethane
22
59
22
59
­
1,2­
Dichloroethane
NL
3
68
211
3
1,
1­
Dichloroethylene
NL
6
16
25
6
1,2­
Dichloropropylene
153
230
153
230
­
1,3­
Dichloropropane
10
20
29
44
­
Methyl
Bromide
20
40
20
40
9
Methyl
Chloride
86
190
86
190
10
Methylene
Chloride
NL
9.4
40
89
6
1,1,2,2
Tetrachloroethane
NL
10
NL
10
10
Tetrachloroethylene
NL
16
22
56
9
1,2­
Trans­
Dichloroethylene
21
54
21
54
4
1,1,1­
Trichloroethane
21
54
21
54
6
1,1,2­
Trichloroethane
NL
12
21
54
6
Trichloroethylene
NL
5.4
21
54
5
Vinyl
Chloride
NL
10
104
268
10
Acid
Compounds
2­
Chlorophenol
31
98
31
98
20
2,4
Dichlorophenol
39
112
39
112
10
2,4
Dimethylphenol
18
36
18
36
­
4,6
Dinitro­
O­
Cresol
NL
60
78
277
60
2,4
Dinitrophenol
71
123
71
123
40
2­
Nitrophenol
41
69
41
69
­
4­
Nitrophenol
72
124
72
124
­
Pentachlorophenol
NL
30
NL
30
30
Phenol
15
26
15
26
10
2,4,6
Trichlorophenol
NL
20
NL
20
20
Base/
Neutral
Compounds
Anthracene
22
59
22
59
10
Benzidine
NL
50
NL
50
50
Benzo
(
a)
Anthracene
NL
10
NL
10
10
Benzo
(
a)
Pyrene
NL
20
NL
20
20
Part
III
­
Attachment
D
Page
6
of
23
pages
GPPC
Master
permit
Renewal
FW2
Waters
SE,
SC
Waters
Parameter
Monthly
/
Daily
Monthly
/
Daily
Avg.
/
Maximum
Avg.
/
Maximum
RQL*
BaseNeutral
Compounds
(
continued)
Benzo
(
b)
fluoranthene
NL
10
NL
10
10
Benzo
(
k)
fluoranthene
NL
20
NL
20
20
Bis
(
2­
Chloroethyl)
Ether
NL
10
NL
10
10
Bis
(
2­
Chloroisopropyl)
Ether
301
757
301
757
10
Bis
(
2­
Ethylhexyl)
Phthalate
NL
36
59
118
30
Butyl
Benzyl
Phthalate
NL
24
NL
24
20
Chrysene
NL
20
NL
20
20
Dibenzo
(
a,
h)
Anthracene
NL
20
NL
20
20
1,2
Dichlorobenzene
77
163
77
163
9
1,3
Dichlorobenzene
31
44
31
44
9
1,4
Dichlorobenzene
NL
28
NL
28
20
3,3
Dichlorobenzidine
NL
60
NL
60
60
Diethyl
Phthalate
81
203
81
203
10
Dimethyl
Phthalate
19
47
19
47
10
Di­
N­
Butyl
Phthalate
27
57
27
57
20
2,4
Dinitrotoluene
NL
10
NL
18.2
10
2,6
Dinitrotoluene
255
641
255
641
­
Fluoranthene
25
68
25
68
10
Fluorene
22
59
22
59
10
Hexachlorobenzene
NL
10
NL
10
10
Hexachlorobutadiene
NL
10
20
49
10
Hexchloropentadiene
240
480
NL
1800
10
Hexachloroethane
19
38
21
54
10
Ideno
(
1,2,3­
cd)
Pyrene
NL
20
NL
20
20
Isophorone
NL
20
NL
20
10
Nitrobenzene
17
34
27
68
10
N­
Nitrosodimethylamine
NL
20
NL
20
20
N­
Nitrosodiphenylamine
NL
20
NL
20
20
Phenanthrene
22
59
22
59
10
Pyrene
25
67
25
67
20
1,2,4
Trichlorobenzene
68
140
68
140
10
Metals
and
Cyanide
Arsenic
**
50
100
50
100
8
Cadmium
**
50
100
50
100
4
Chromium
**
50
100
50
100
10
Copper
**
50
100
50
100
10
Mercury
**
NL
1
NL
1
1
Nickel
**
72
144
50
100
10
Selenium
**
50
100
50
100
10
Silver
**
25
50
25
50
2
Zinc
**
100
200
100
200
30
Cyanide
100
200
100
200
40
*
The
permittee
shall
ensure
that
analytical
data
is
sampled
at
detection
levels
as
sensitive
as
the
Recommended
Quantitation
Levels
(
RQL's)
for
any
of
the
above
parameters
limited
in
the
individual
authorization.
**
If
this
parameter
is
regulated
in
the
individual
authorization,
a
chronic
WET
limit
is
also
applicable.
Part
III
­
MTBE
Reporting
Guidance
Page
7
of
23
pages
GPPC
Master
Permit
Renewal
Clarification
of
Reporting
Requirements
for
MTBE
Percent
Removal
The
equation
for
reporting
MTBE
percent
removal
is
as
follows:

MTBE
%
Removal
=
MTBE
Influent
 
MTBE
Effluent
x
100
MTBE
Influent
The
equation
above
assumes
that
the
permittee
is
monitoring
MTBE
influent
and
effluent
only
once
during
the
calendar
month
as
required
in
the
master
general
permit.
Permittees
can
always
monitor
more
frequently
than
specified
in
the
NJPDES
permit
as
it
encourages
more
representative
data.
In
the
event
that
a
permittee
monitors
MTBE
more
than
once
during
a
particular
calendar
month,
calculate
individual
percent
removal
values
for
each
data
set
(
influent
and
effluent)
and
divide
by
the
number
of
data
sets
available
to
obtain
the
MTBE
%
removal
value.

Note:
Although
examples
are
given
below
for
all
tables
included
in
Part
III
of
the
master
GPPC
permit,
discharges
are
typically
authorized
under
only
one
table
in
individual
authorizations.
Therefore,
only
one
of
the
five
tables
(
A,
B,
C,
D
or
E)
of
Part
III
is
typically
appropriate
for
a
subject
facility.

Given
the
above
equation,
reporting
procedures
are
as
follows
for
detectable
and
non­
detectable
or
unquantified
values:

 
If
the
influent
value
is
detected
and
quantified
and
the
effluent
value
is
detected
and
quantified
and
only
one
sample
of
each
is
taken
during
the
calendar
month,
complete
the
calculation
using
the
detected
values.
For
example:

MTBE
%
Removal
=
MTBE
Influent
 
MTBE
Effluent
x
100
MTBE
Influent
MTBE
Influent
=
680
ug/
L
­
only
influent
sample
taken
during
the
calendar
month
MTBE
Effluent
=
90
ug/
L
­
only
effluent
sample
taken
during
the
calendar
month
MTBE
%
Removal
=
680
­
90
x
100
=
86.7647
Report
87
%
on
monitoring
report
form.
680
 
If
the
influent
value
is
detected
and
quantified
and
the
effluent
value
is
non­
detectable
or
unquantified
and
only
one
sample
of
each
is
taken
during
the
calendar
month,
substitute
one­
half
of
the
detection
limit
in
the
equation
for
the
unquantified
value.
For
example:

MTBE
%
Removal
=
MTBE
Influent
 
MTBE
Effluent
x
100
MTBE
Influent
MTBE
Influent
=
50
ug/
L
­
only
influent
sample
taken
during
the
calendar
month
MTBE
Effluent
=
<
5
ug/
L
­
only
effluent
sample
taken
during
the
calendar
month
MTBE
%
Removal
=
50
 
2.5
x
100
=
95
50
For
discharges
authorized
under
Tables
A,
D
and
E:
Because
the
effluent
value
is
less
than
70
ug/
L,
the
MTBE
minimum
percent
removal
limitation
does
not
apply,
and
the
permittee
shall
report
"
Code
=
N"
on
its
monthly
monitoring
report
form
consistent
with
the
instructions
indicated
in
Part
IV.

For
discharges
authorized
under
Tables
B
and
C:
Report
95%
on
monitoring
report
form.
Part
III
­
MTBE
Reporting
Guidance
Page
8
of
23
pages
GPPC
Master
Permit
Renewal
 
If
the
influent
value
is
non­
detectable
or
unquantified
and
the
effluent
value
is
detected
and
quantified
and
only
one
sample
of
each
is
taken
during
the
calendar
month,
substitute
one­
half
of
the
detection
limit
in
the
equation
for
the
unquantified
value.
For
example:

MTBE
%
Removal
=
MTBE
Influent
 
MTBE
Effluent
x
100
MTBE
Influent
MTBE
Influent
=
<
10
ug/
L
­
only
influent
sample
taken
during
the
calendar
month
MTBE
Effluent
=
11
ug/
L
­
only
effluent
sample
taken
during
the
calendar
month
MTBE
%
Removal
=
5
 
11
x
100
=
­
120
5
For
discharges
authorized
under
Table
A,
D
and
E:
Because
the
effluent
value
is
less
than
70
ug/
L,
the
MTBE
minimum
percent
removal
limitation
does
not
apply,
and
the
permittee
shall
report
"
Code
=
N"
on
its
monthly
monitoring
report
form
consistent
with
the
instructions
indicated
in
Part
IV.

For
discharges
authorized
under
Tables
B
and
C:
Report
 
120%.

 
If
the
influent
value
is
non­
detectable
or
unquantified
and
the
effluent
value
is
non­
detectable
or
unquantified
and
only
one
sample
of
each
is
taken
during
the
calendar
month,
substitute
0
for
both
influent
and
effluent
values.
For
example:

MTBE
%
Removal
=
MTBE
Influent
 
MTBE
Effluent
x
100
MTBE
Influent
MTBE
Influent
=
<
10
ug/
L
­
only
influent
sample
taken
during
the
calendar
month
MTBE
Effluent
=
<
5
ug/
L
­
only
effluent
sample
taken
during
the
calendar
month
MTBE
%
Removal
=
0
­
0
x
100
=
0
0
For
discharges
authorized
under
Table
A,
D
and
E:
Because
the
effluent
value
is
less
than
70
ug/
L,
the
MTBE
minimum
percent
removal
limitation
does
not
apply,
and
the
permittee
shall
report
"
Code
=
N"
on
its
monthly
monitoring
report
form
consistent
with
the
instructions
indicated
in
Part
IV.

For
discharges
authorized
under
Tables
B
and
C:
Report
0%.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
9
of
23
pages
APPENDIX
A:

CHRONIC
TOXICITY
TESTING
SPECIFICATIONS
FOR
USE
IN
THE
NJPDES
PERMIT
PROGRAM
Version
2.1
May
1997
(
This
Section
is
only
applicable
if
a
chronic
toxicity
limit
is
required
as
specified
in
Part
III.
Chronic
toxicity
limits
are
applied
only
under
Table
D
and
if
metals
are
present
at
certain
levels.)
Appendix
A
­
Chronic
Toxicity
Specifications
Page
10
of
23
pages
TABLE
OF
CONTENTS
I.
AUTHORITY
AND
PURPOSE
II.
GENERAL
CONDITIONS
A.
Laboratory
Safety
and
Glassware
B.
Test
Concentrations
/
Replicates
C.
Dilution
Water
D.
Effluent
Sample
Collection
E.
Physical
Chemical
Measurements
F.
Statistics
III.
TEST
ACCEPTABILITY
CRITERIA
IV.
STANDARD
REFERENCE
TOXICANT
TESTING
A.
Initial
Testing
Requirements
B.
Subsequent
Testing
Requirements
C.
Changing
an
Established
Reference
Toxicant
D.
Control
Charts
E.
Unacceptable
SRT
Results
F.
Annual
Submittals
V.
TEST
CANCELLATION
/
RESCHEDULING
EVENTS
VI.
REPORTING
VII.
METHODS
SPECIFICATIONS
A.
Fathead
Minnow
(
Pimephales
promelas),
Larval
Survival
and
Growth
Test,
method
1000.0
B.
Ceriodaphnia
dubia,
Survival
and
Reproduction
Test,
method
1002.0
C.
Algal,
(
Selenastrum
capricornutum),
Growth
Test,
method
1003.0
D.
Sheepshead
Minnow
(
Cyprinodon
variegatus),
Larval
Survival
and
Growth
Test,
method
1005.0
E.
Inland
Silverside
(
Menidia
beryllina),
Larval
Survival
and
Growth
Test,
method
1006.0
F.
Mysidopsis
bahia,
Survival,
Growth,
and
Fecundity
Test,
method
1007.0
G.
Champia
parvula,
Sexual
Reproduction
Test,
method
1009.0
VIII.
REFERENCES
Notice:
Mention
of
trade
names
or
commercial
products
do
not
constitute
endorsement
or
recommendation
for
use.
i
Appendix
A
­
Chronic
Toxicity
Specifications
Page
11
of
23
pages
I.
AUTHORITY
AND
PURPOSE
These
methods
specifications
for
the
conduct
of
whole
effluent
chronic
toxicity
testing
are
established
under
the
authority
of
the
NJPDES
permitting
program,
N.
J.
A.
C.
7:
14A­
6.5(
a)
2
and
40
CFR
136,
for
discharges
to
waters
of
the
State.
The
methods
referenced
herein
are
included
by
reference
in
40
CFR
136,
Table
1.
A.
and,
therefore,
constitute
approved
methods
for
chronic
toxicity
testing.
The
information
contained
herein
serves
to
clarify
testing
requirements
not
sufficiently
clarified
in
those
methods
documents
and
also
serves
to
outline
and
implement
the
interlaboratory
Standard
Reference
Toxicant
Program
until
a
formal
laboratory
certification
program
is
established
under
N.
J.
A.
C.
7:
18.
As
such
these
methods
are
intended
to
be
used
to
determine
compliance
with
discharge
permits
issued
under
the
authority
of
the
NJPDES
permit
program.
Tests
are
to
be
conducted
in
accordance
with
the
general
conditions
and
test
organism
specific
method
specifications
contained
in
this
document.
All
other
conditions
and
specifications
can
be
found
in
40
CFR
136
and
USEPA
methodologies.

Until
a
subchapter
on
chronic
toxicity
testing
within
the
regulations
governing
the
certification
of
laboratories
and
environmental
measurements
(
N.
J.
A.
C.
7:
18)
becomes
effective,
tests
shall
be
conducted
in
conformance
with
the
methodologies
as
designated
herein
and
contained
in
40
CFR
136.
The
laboratory
performing
the
testing
shall
be
within
the
existing
acute
toxicity
testing
laboratory
certification
program
established
under
N.
J.
A.
C.
7:
18,
as
required
by
N.
J.
A.
C.
7:
9B­
1.5(
c)
5.

Testing
shall
be
in
conformance
with
the
subchapter
on
chronic
toxicity
testing
within
the
N.
J.
A.
C.
7:
18
when
such
regulations
become
effective.
The
laboratory
performing
the
toxicity
testing
shall
be
within
the
chronic
toxicity
testing
laboratory
certification
program
to
be
established
under
that
subchapter,
when
it
becomes
effective.

These
methods
are
incorporated
into
discharge
permits
as
enforceable
permit
conditions.
Each
discharge
permit
will
specify
in
Part
IV
of
the
permit,
the
test
species
specific
methods
from
this
document
that
will
be
required
under
the
terms
of
the
discharge
permit.
Although
the
test
species
specific
methods
for
each
permit
are
determined
on
a
case­
by­
case
basis,
the
purpose
of
this
methods
document
is
to
assure
consistency
among
dischargers
and
to
provide
certified
laboratories
with
information
on
the
universe
of
tests
to
be
utilized
so
that
they
can
make
the
necessary
preparations,
including
completing
the
required
Standard
Reference
Toxicant
testing.
Please
note
that
these
methodologies
are
required
for
compliance
testing
only.
Facilities
and/
or
laboratories
conducting
testing
under
the
requirements
of
a
Toxicity
Identification
Evaluation
or
for
informational
purposes
are
not
bound
by
these
methods.

This
document
constitutes
the
second
version
of
the
NJDEP's
interim
chronic
methodologies.
This
version
contains
no
significant
changes
to
the
test
methods
themselves.
However,
in
keeping
with
the
Department's
continued
emphasis
on
good
laboratory
practices
and
quality
control,
the
areas
addressing
the
Standard
Reference
Toxicant
Program,
data
analysis
and
data
reporting,
have
been
significantly
revised.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
12
of
23
pages
II.
GENERAL
CONDITIONS
A.
LABORATORY
SAFETY,
GLASSWARE,
ETC.

All
safety
procedures,
glassware
cleaning
procedures,
etc.,
shall
be
in
conformance
with
40
CFR
136
and
USEPA's
"
Short
Term
Methods
for
Estimating
the
Chronic
Toxicity
of
Effluents
and
Receiving
Waters
to
Freshwater
Organisms,"
"
Short
Term
Methods
for
Estimating
the
Chronic
Toxicity
of
Effluents
and
Receiving
Waters
to
Marine
and
Estuarine
Organisms"
and
N.
J.
A.
C.
7:
18.

B.
TEST
CONCENTRATIONS
/
REPLICATES
All
testing
is
to
be
performed
with
a
minimum
of
five
effluent
concentrations
plus
a
dilution
water
control.
A
second
reference
water
control
is
optional
when
a
dilution
water
other
than
culture
water
is
used.
The
use
of
both
a
0.5
or
0.75
dilution
factor
is
acceptable
for
the
selection
of
test
concentrations.
If
hypothesis
testing
will
be
used
to
determine
the
test
endpoint,
one
effluent
concentration
shall
be
the
chronic
permit
limitation,
unless
the
existing
data
for
the
discharge
indicate
that
the
NOEC
is
expected
to
be
significantly
less
than
the
permit
limit.
The
use
of
the
0.5
dilution
factor
may
require
more
than
five
dilutions
to
cover
the
entire
range
of
effluent
concentrations
as
well
as
the
chronic
permit
limit,
since
the
permit
limit
will
often
not
be
one
of
the
nominal
concentrations
in
a
0.5
dilution
series.
In
such
an
instance,
the
0.5
dilution
series
may
be
altered
by
including
an
additional
test
concentration
equal
to
the
permit
limit
in
the
dilution
series,
or
by
changing
the
concentration
closest
to
the
permit
toxicity
limit
to
be
equal
to
that
limit.
The
Department
recommends
the
use
of
the
0.75
dilution
factor
using
Table
1.0
to
determine
test
concentrations.
That
table
establishes
test
concentrations
based
on
the
chronic
toxicity
limitation.

For
either
the
0.5
or
0.75
dilution
factor,
there
shall
be
at
least
one
test
concentration
above
the
permit
limitation
and
at
least
three
test
concentrations
below
the
permit
limit
along
with
the
dilution
water
control
unless
the
permit
limitation
prohibits
such
(
e.
g.,
limitations
greater
than
75%
effluent).
An
effort
shall
be
made
to
bracket
the
anticipated
test
result.

To
use
Table
1.0,
locate
the
permit
limit
in
column
4.
The
dilution
series
becomes
the
row
that
corresponds
to
the
permit
limit
in
column
4.
For
example,
a
permit
limit
of
41
would
require
a
dilution
series
of
the
dilution
water
control,
17%,
23%,
31%,
41%
and
55%
effluent.

The
number
of
replicates
used
in
the
test
must,
at
a
minimum,
satisfy
the
specifications
of
the
applicable
methods
contained
herein.
Increased
data
sensitivity
can
be
obtained
by
increasing
the
number
of
replicates
equally
among
test
concentrations
and
thus
an
increased
number
of
replicates
is
acceptable.
Further,
the
use
of
nonparametric
statistical
analysis
requires
a
minimum
of
four
replicates
per
test
concentration.
If
the
data
for
any
particular
test
is
not
conducive
to
parametric
analyses
and
if
less
than
four
replicates
were
included,
the
test
may
not
be
considered
acceptable
for
compliance
purposes.

The
use
of
single
concentration
tests
consisting
of
the
permit
limitation
as
a
concentration
and
a
control
is
not
permitted
for
compliance
purposes,
but
may
be
used
by
a
permittee
in
the
conduct
of
a
Toxicity
Investigation
Evaluation
(
TIE)
or
for
information
gathering
purposes.
Such
a
test
would
be
considered
a
"
pass"
if
there
was
no
significant
difference
in
test
results,
using
hypothesis
testing
methods.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
13
of
23
pages
Table
1.0:
0.75
DILUTION
SERIES
INDEXED
BY
PERMIT
LIMIT
Permit
Limit
Permit
Limit
Col
#
1
2
3
4
5
Col
#
1
2
3
4
5
0.4
0.6
0.8
1
1.3
22
29
38
51
68
0.8
1.1
1.5
2
2.7
22
29
39
52
69
1.3
1.7
2.3
3
4
22
30
40
53
71
1.7
2.3
3
4
5.3
23
30
41
54
72
2.1
2.8
3.8
5
6.7
23
31
41
55
73
2.5
3.4
4.5
6
8
24
32
42
56
75
3
4
5
7
9
24
32
43
57
76
3
5
6
8
11
24
33
44
58
77
4
5
7
9
12
25
33
44
59
79
4
6
8
10
13
25
34
45
60
80
5
6
8
11
15
26
34
46
61
81
5
7
9
12
16
26
35
47
62
83
5
7
10
13
17
27
35
47
63
84
6
8
11
14
19
27
36
48
64
85
6
8
11
15
20
27
37
49
65
87
7
9
12
16
21
28
37
50
66
88
7
10
13
17
23
28
38
50
67
89
8
10
14
18
24
29
38
51
68
91
8
11
14
19
25
29
39
52
69
92
8
11
15
20
27
30
39
53
70
93
9
12
16
21
28
30
40
53
71
95
9
12
17
22
29
30
41
54
72
96
10
13
17
23
31
31
41
55
73
97
10
14
18
24
32
31
42
56
74
99
11
14
19
25
33
32
42
56
75
100
11
15
20
26
35
24
32
43
57
76
11
15
20
27
36
24
32
43
58
77
12
16
21
28
37
25
33
44
59
78
12
16
22
29
39
25
33
44
59
79
13
17
23
30
40
25
34
45
60
80
13
17
23
31
41
26
34
46
61
81
14
18
24
32
43
26
35
46
62
82
14
19
25
33
44
26
35
47
62
83
14
19
26
34
45
27
35
47
63
84
15
20
26
35
47
27
36
48
64
85
15
20
27
36
48
27
36
48
65
86
16
21
28
37
49
28
37
49
65
87
16
21
29
38
51
28
37
50
66
88
16
22
29
39
52
28
38
50
67
89
17
23
30
40
53
28
38
51
68
90
17
23
31
41
55
29
38
51
68
91
18
24
32
42
56
29
39
52
69
92
18
24
32
43
57
29
39
52
70
93
19
25
33
44
59
30
40
53
71
94
19
25
34
45
60
30
40
53
71
95
19
26
35
46
61
30
41
54
72
96
20
26
35
47
63
31
41
55
73
97
20
27
36
48
64
31
41
55
74
98
21
28
37
49
65
31
42
56
74
99
21
28
38
50
67
32
42
56
75
100
*
Select
the
dilution
series
by
finding
the
row
which
contains
the
permit
limit
in
column
#
4.
NOTE:
All
values
are
in
units
of
"%
effluent"
not
toxic
units.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
14
of
23
pages
C.
DILUTION
WATER
1.
Marine
and
Estuarine
Waters
A
high
quality
natural
water,
such
as
the
Manasquan
River
Inlet
is
strongly
recommended
as
the
dilution
water
source
for
chronic
toxicity
testing
with
marine
and
estuarine
organisms.
The
use
of
the
receiving
water
as
the
dilution
water
source
is
not
required.
Saline
waters
prepared
with
hypersaline
brine
and
deionized
water
may
also
be
used
as
dilution
water.
Hypersaline
brines
shall
be
prepared
from
a
high
quality
natural
seawater
and
shall
not
exceed
a
concentration
of
100
ppt.
The
type
of
a
dilution
water
for
a
permittee
may
not
be
changed
without
the
prior
approval
of
the
Department.

The
standard
test
salinity
shall
be
25
ppt,
except
for
Champia
parvula,
which
shall
be
tested
at
30
ppt.
Since
most
effluents
are
freshwater
based,
in
most
cases
it
will
be
necessary
to
adjust
the
salinity
of
the
test
concentrations
to
the
standard
test
salinity.

2.
Fresh
Waters
A
high
quality
natural
water,
such
as
Round
Valley
Reservoir
(
if
access
is
allowed)
or
Lake
Hopatcong,
is
strongly
recommended
as
the
dilution
water
source
for
chronic
toxicity
testing
with
freshwater
organisms.
It
is
not
required
to
perform
the
toxicity
testing
with
the
receiving
water
as
dilution
water.
Tests
performed
with
a
reconstituted
water
or
up
to
20%
Diluted
Mineral
Water
(
DMW)
as
dilution
water
is
acceptable.
For
testing
with
Ceriodaphnia
dubia,
the
addition
of
5
µ
g/
l
selenium
(
2
µ
g/
l
selenium
with
natural
water)
and
1
µ
g/
l
vitamin
B12
is
recommended
(
Keating
and
Dagbusan,
1984:
Keating,
1985
and
1988).
The
source
of
a
dilution
water
for
a
permittee
may
not
be
changed
without
the
prior
approval
of
the
Department.
Reconstituted
water
and
DMW
should
be
prepared
with
Millipore
Super
QR
or
equivalent,
meet
the
requirements
of
N.
J.
A.
C.
7:
18­
6
and
should
be
aerated
a
minimum
of
24
hrs
prior
to
use,
but
not
supersaturated.

D.
EFFLUENT
SAMPLE
COLLECTION
Effluent
samples
shall
be
representative
of
the
discharge
being
regulated.
For
each
discharge
serial
number
(
DSN),
the
effluent
sampling
location
shall
be
the
same
as
that
specified
in
the
NJPDES
permit
for
other
sampling
parameters
unless
an
alternate
sampling
point
is
specified
in
the
NJPDES
discharge
permit.
For
industrial
dischargers
with
a
combined
process/
sanitary
waste
stream,
effluent
sampling
shall
be
after
chlorination,
unless
otherwise
designated
in
the
permit.

For
continuous
discharges,
effluent
sampling
shall
consist
of
24
hour
composite
samples
consisting
either
of
equal
volumes
taken
once
every
hour
or
of
a
flow­
proportionate
composite
sample,
unless
otherwise
approved
by
the
Department.
At
a
minimum,
three
samples
shall
be
collected
as
specified
above,
one
every
other
day.
The
first
sample
shall
be
used
for
test
initiation
and
the
first
renewal.
The
second
sample
for
the
next
two
renewals.
The
third
sample
shall
be
used
for
the
final
three
renewals.
For
the
Champia
and
Selenastrum
tests,
a
single
sample
shall
be
collected
not
more
than
24
hours
prior
to
test
initiation.
No
effluent
sample
shall
be
over
72
hours
old
at
the
time
of
its
use
to
initiate
or
renew
solutions
in
a
test.
It
is
acceptable
to
collect
samples
more
frequently
for
chronic
WET
testing
and
if
samples
are
collected
daily
for
acute
toxicity
testing
conducted
concurrently,
available
samples
may
be
used
to
renew
the
test
solutions
as
appropriate.

For
all
other
types
of
discharges,
effluent
sampling
shall
be
conducted
according
to
specifications
contained
within
the
discharge
permit,
methodology
questionnaire
or
as
otherwise
specified
by
the
Department.
The
use
of
grab
samples
or
other
special
sampling
procedures
will
be
based
on
time
of
occurrence
and
duration
of
intermittent
discharge
events.

If
a
municipal
discharger
has
concerns
that
the
concentrations
of
ammonia
and/
or
chlorine
in
an
effluent
are
adequate
to
cause
violations
of
the
permit
limit
for
chronic
toxicity
testing,
the
permittee
should
conduct
analyses,
as
specified
in
USEPA's
toxicity
investigation
methods
documents,
to
illustrate
the
relationship
between
chronic
effluent
toxicity
and
chlorine
and/
or
ammonia
as
applicable.
This
data
may
then
be
submitted
to
the
Department
as
Appendix
A
­
Chronic
Toxicity
Specifications
Page
15
of
23
pages
justification
for
a
request
to
use
modified
test
procedures,
which
account
for
ammonia
and/
or
chlorine
toxicity,
in
future
chronic
toxicity
tests.
The
Department
may,
where
adequate
justification
exists,
permit
the
adjustment
of
these
pollutants
in
the
effluent
sample
if
discharge
limits
for
these
pollutants
are
contained
in
the
NJPDES
permit
and
those
permit
limitations
are
adequate
for
the
protection
of
water
quality.
Any
proposed
modified
test
procedures
to
adjust
effluent
chlorine
and/
or
ammonia
shall
be
approved
by
the
Department
prior
to
use
of
those
test
procedures
for
any
compliance
testing.

Except
for
filtration
through
a
2
mm
or
larger
screen
or
an
adjustment
to
the
standard
test
salinity,
no
other
adjustments
to
the
effluent
sample
shall
be
made
without
prior
written
approval
by
the
Department.
Aeration
of
samples
prior
to
test
start
shall
be
minimized
where
possible
and
samples
shall
not
be
aerated
where
adequate
saturation
exists
to
maintain
dissolved
oxygen.

E.
PHYSICAL
CHEMICAL
MEASUREMENTS
At
a
minimum,
the
physical
chemical
measurements
shall
be
as
follows:

°
pH
and
dissolved
oxygen
shall
be
measured
at
the
beginning
and
end
of
each
24
hour
exposure
period,
in
at
least
one
chamber,
of
the
high,
medium
and
low
test
concentrations
and
the
control.
In
order
to
ensure
that
measurements
for
these
parameters
are
representative
of
the
test
concentrations
during
the
test,
measurements
for
these
parameters
should
be
taken
in
an
additional
replicate
chamber
for
such
concentrations
which
contains
no
test
organisms,
but
is
subject
to
the
same
test
conditions.

°
Temperature
shall
either
be
monitored
continuously,
measured
daily
in
at
least
two
locations
in
the
environmental
control
system,
or
measured
at
the
beginning
of
each
24
hr
exposure
period
in
at
least
one
replicate
for
each
treatment.

°
Salinity
shall
be
measured
in
all
salt
water
tests
at
the
beginning
of
each
24
hour
exposure
period,
in
at
least
one
replicate
for
each
treatment.

°
For
all
freshwater
tests,
alkalinity,
hardness
and
conductivity
shall
be
measured
in
each
new
sample
(
100%
effluent)
and
control.

°
Nitrite,
nitrate
and
ammonia
shall
be
measured
in
the
control
before
each
renewal
in
the
mysid
test
only.

°
For
samples
of
discharges
where
concentrations
of
ammonia
and/
or
chlorine
are
known
or
are
suspected
to
be
sufficient
to
cause
toxicity,
it
is
recommended
that
the
concentrations
of
these
pollutants
be
determined
and
submitted
with
the
standardized
report
form.
The
laboratory
is
advised
to
consult
with
the
permittee
to
determine
if
these
parameters
should
be
measured
in
the
effluent.
Where
such
measurements
are
deemed
appropriate,
measurements
shall
be
conducted
at
the
beginning
of
each
24
hour
exposure
period.
Also,
since
a
rise
in
the
test
pH
can
affect
the
toxicity
of
ammonia
in
the
effluent,
analysis
of
ammonia
during
the
test
may
be
appropriate
if
a
rise
in
pH
is
accompanied
by
a
significant
increase
in
mortality.

F.
STATISTICS
The
use
of
both
hypothesis
testing
techniques
and
point
estimate
techniques
are
currently
in
use
by
the
Department
or
by
permittees
for
compliance
purposes.
The
NJPDES
permit
should
be
checked
to
determine
which
type
of
analysis
is
required
and
appropriate
for
each
specific
facility.
It
is
not
acceptable
to
simply
evaluate
any
data
by
"
visual
data
review"
unless
in
the
analysis
of
survival
data,
no
mortality
occurred
in
the
test.
All
data
sets
must
be
appropriately
statistically
evaluated.

For
hypothesis
testing
techniques,
statistical
analysis
shall
follow
the
protocols
in
USEPA
(
1988,
1989)
to
evaluate
adverse
effects.
A
significance
level
of
0.05
shall
be
utilized
to
evaluate
such
effects.
Use
of
a
protocol
not
Appendix
A
­
Chronic
Toxicity
Specifications
Page
16
of
23
pages
contained
in
these
documents
must
be
accompanied
by
a
reference
and
explanation
addressing
its
applicability
to
the
particular
data
set.
Please
note
the
following
when
evaluating
data
using
hypothesis
testing
techniques.

Special
attention
should
be
given
to
the
omission
and
inclusion
of
a
given
replicate
in
the
analysis
of
mysid
fecundity
data
(
USEPA
1994,
p.
275)
and
Ceriodaphnia
reproduction
data
(
USEPA
1994,
page
174).

Determination
of
acceptability
criteria
and
average
individual
dry
weight
for
the
growth
endpoints
must
follow
the
specifications
in
the
applicable
documents
(
e.
g.,
p.
84
for
saltwater
methods
document.)

Use
of
nonparametric
statistical
analyses
requires
a
minimum
of
four
replicates
per
test
concentration.
If
the
data
for
any
particular
test
are
not
conducive
to
parametric
analyses
and
if
less
than
four
replicates
were
included,
the
test
may
not
be
acceptable
to
the
Department.

Where
hypothesis
testing
is
used
for
compliance
purposes,
if
the
results
of
hypothesis
testing
indicate
that
a
deviation
from
the
dose
response
occurs
such
that
two
test
concentrations
are
deemed
statistically
significant
from
the
control
but
an
intermediate
test
concentration
is
not,
the
test
is
deemed
unacceptable
and
cannot
be
used
for
compliance
testing
purposes.

For
point
estimate
techniques,
statistical
analysis
should
follow
the
protocol
contained
in
"
A
Linear
Interpolation
Method
for
Sublethal
Toxicity:
The
Inhibition
Concentration
(
ICp)
Approach
(
Version
2.0),
July
1993,
National
Effluent
Toxicity
Assessment
Center
Technical
Report
03­
93."
Copies
of
the
program
can
be
obtained
by
contacting
the
Department.
The
linear
interpolation
estimate
ICp
values
and
not
the
bootstrap
mean
ICp,
shall
be
reported
for
permit
compliance
purposes.
The
ICp
value
reported
on
the
Discharge
Monitoring
Report
shall
be
rounded
off
as
specified
in
the
Department's
"
Discharge
Monitoring
Report
(
DMR)
Instruction
Manual,
December
1993."
IC25
values
shall
be
reported
under
the
parameter
code
listed
as
"
NOEC"
on
the
DMR,
until
the
DMR's
are
adjusted
accordingly.

If
the
result
reported
by
the
ICp
method
is
greater
than
the
highest
concentration
tested,
the
test
result
is
reported
as
"
greater
than
C"
where
"
C"
is
the
highest
tested
concentration.
If
the
ICp
is
lower
than
the
lowest
concentration
tested,
the
test
result
is
reported
as
"
less
than
C"
where
"
C"
is
the
lowest
tested
concentration.

If
separate
NOEC's/
IC25'
s
can
be
calculated
from
multiple
test
endpoints,
for
example
a
reproductive
endpoint
and
a
growth
endpoint,
the
lowest
NOEC/
IC25
value
expressed
in
units
of
"%
effluent"
will
be
used
to
determine
permit
compliance
and
should,
therefore,
be
reported
as
the
NOEC/
IC25
value
for
the
test.
If
the
NOEC
value
for
growth
and/
or
reproduction
is
not
lower
than
that
for
survival,
the
NOEC/
IC25
value
reported
for
the
test
shall
be
as
survival.
For
saltwater
tests,
where
additional
controls
are
used
in
a
test
(
i.
e.
brine
and/
or
artificial
sea
salt
control),
a
T­
test
shall
be
used
to
determine
if
there
is
a
significant
difference
between
the
original
test
control
and
the
additional
controls.
If
there
is
a
significant
difference
between
any
of
the
controls,
the
test
may
be
deemed
unacceptable
and
if
so,
will
not
be
used
for
permit
compliance.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
17
of
23
pages
III.
TEST
ACCEPTABILITY
CRITERIA
Any
test
that
does
not
meet
these
acceptability
criteria
will
not
be
used
by
the
Department
for
any
purpose
and
must
be
repeated
as
soon
as
practicable,
with
a
freshly
collected
sample.

1.
Tests
must
be
performed
by
a
laboratory
approved
for
the
conduct
of
chronic
toxicity
tests
and
certified
for
acute
toxicity
testing
under
N.
J.
A.
C.
7:
18.

2.
Test
results
may
be
rejected
due
to
inappropriate
sampling,
including
the
use
of
less
than
three
effluent
samples
in
a
test
and/
or
use
of
procedures
not
specified
in
a
permit
or
methodology
questionnaire,
use
of
frozen
or
unrefrigerated
samples
or
unapproved
pretreatment
of
an
effluent
sample.

3.
Controls
shall
meet
the
applicable
performance
criteria
specified
in
the
Table
2.0
and
in
the
individual
method
specifications
contained
herein.

4.
Acceptable
and
applicable
Standard
Reference
Toxicant
Data
must
be
available
for
the
test.

5.
No
unapproved
deviations
from
the
applicable
test
methodology
may
be
present.

6.
When
using
hypothesis
testing
techniques,
a
deviation
from
the
dose
response
as
explained
in
the
statistical
portion
of
this
document
shall
not
be
present
in
the
data.

THE
DETERMINATION
OF
A
TEST
AS
UNACCEPTABLE
DOES
NOT
RELIEVE
THE
FACILITY
FROM
MONITORING
FOR
THAT
MONITORING
PERIOD
Table
2.0:
CONTROL
PERFORMANCE
TEST
ORGANISM
MINIMUM
SURVIVAL
MINIMUM
WEIGHT
GAIN
MINIMUM
FECUNDITY/
REPRODUCTION
Pimephales
promelas
80%
0.25
mg
avg
N/
A
Ceriodaphnia
dubia
80%
N/
A
Average
of
>
15
young
per
surviving
female
Selenastrum
capricornutum
Density
>
2x105cells/
ml
N/
A
Variability
in
controls
not
to
exceed
20%.

Cyprinodon
variegatus
80%
0.60
mg
(
unpreserved)
avg
0.50
mg
(
preserved)
avg
N/
A
Menidia
beryllina
80%
0.50
mg
(
unpreserved)
avg
0.43
mg
(
preserved)
avg
N/
A
Mysidopsis
bahia
80%
0.2
mg
per
mysid
avg
egg
production
by
50%
of
control
females
if
fecundity
is
used
as
an
endpoint.
Champia
parvula
100%
N/
A
>
10
cystocarps
per
plant
Plants
in
controls
and
lower
test
concentrations
shall
not
fragment
so
that
individual
plants
cannot
be
identified.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
18
of
23
pages
IV.
STANDARD
REFERENCE
TOXICANT
TESTING
All
chronic
testing
shall
be
accompanied
by
testing
with
a
Standard
Reference
Toxicant
(
SRT)
as
a
part
of
each
laboratory's
internal
quality
control
program.
Such
a
testing
program
should
be
consistent
with
the
quality
assurance/
quality
control
protocols
described
in
the
USEPA
chronic
testing
manuals.
Laboratories
may
utilize
the
reference
toxicant
of
their
choice
and
toxicants
such
as
cadmium
chloride,
potassium
chloride,
sodium
dodecyl
sulfate
and
copper
sulfate
are
all
acceptable.
However,
Potassium
chloride
has
been
chosen
by
several
laboratories
and
is
recommended
by
the
Department.
The
concentration
of
the
reference
toxicant
shall
be
verified
by
chemical
analysis
in
the
low
and
high
test
concentrations
once
each
year
or
every
12
tests,
whichever
is
less.
It
is
not
necessary
to
run
SRT
tests,
for
all
species
using
the
same
SRT.

A.
INITIAL
STANDARD
REFERENCE
TOXICANT
(
SRT)
TESTING
REQUIREMENTS
At
a
minimum,
this
testing
shall
include
an
initial
series
of
at
least
five
SRT
tests
for
each
test
species
method.
Acceptable
SRT
testing
for
chronic
toxicity
shall
be
performed
utilizing
the
short
term
chronic
toxicity
test
methods
as
specified
herein.
Reference
toxicant
tests
utilizing
acute
toxicity
testing
methods,
or
any
method
other
than
those
contained
in
this
document
are
not
acceptable.
The
laboratory
should
forward
results
of
the
initial
SRT
testing,
including
control
charts,
the
name
of
the
reference
toxicant
utilized,
the
supplier
and
appropriate
chemical
analysis
of
the
toxicant
to
either
address
listed
in
the
reporting
requirements
section
herein.
The
initial
series
of
a
least
five
SRT
tests
for
a
specific
test
species
method
shall
be
completed
and
approved
in
writing
by
the
Department
prior
to
the
conduct
of
any
chronic
toxicity
testing
for
compliance
purposes.

B.
SUBSEQUENT
SRT
TESTING
REQUIREMENTS
After
receiving
the
initial
approval
from
the
Department
to
conduct
chronic
toxicity
tests
for
compliance
purposes,
subsequent
SRT
testing
shall
be
conducted
as
follows:

1.
Where
organisms
used
in
testing
are
cultured
at
the
testing
laboratory,
SRT
testing
should
be
conducted
once
per
month
for
each
species/
method.

2.
Where
the
laboratory
purchases
organisms
from
a
laboratory
certified
in
New
Jersey
for
the
conduct
of
acute
toxicity
testing
and
approved
for
the
conduct
of
chronic
toxicity
testing
for
the
test
organism
in
question
(
i.
e.
the
"
supplier
laboratory"),
SRT
data
provided
by
the
"
supplier
laboratory"
for
each
lot
of
organisms
purchased
is
acceptable
as
long
as
the
SRT
test
result
falls
within
the
control
limits
of
the
control
chart
established
by
the
"
supplier
laboratory"
for
that
organism.
The
laboratory
using
purchased
organisms
is
responsible
for
the
results
of
any
compliance
tests
they
perform.

3.
A
testing
laboratory
purchasing
organisms
from
a
supplier
laboratory
must
still
perform
SRT
testing
on
a
quarterly
basis
at
a
minimum,
for
each
species
they
test
with,
in
order
to
adequately
document
their
own
interlaboratory
precision.

4.
If
a
testing
laboratory
purchasing
organisms
elects
not
to
use
the
SRT
data
from
a
"
supplier
laboratory"
or
such
data
is
unavailable
or
where
organisms
are
purchased
from
another
organism
supplier,
the
testing
laboratory
must
conduct
SRT
testing
on
each
lot
of
organisms
purchased.

5.
For
industrial
laboratories
certified
under
N.
J.
A.
C.
7:
18
to
conduct
acute
toxicity
tests,
only
the
SRT
testing
conditions
specified
in
2.
through
4.
above
apply.
Where
that
laboratory/
facility
cultures
their
own
test
organisms,
the
frequency
of
SRT
testing
required
will
be
determined
on
a
case
by
case
basis,
based
on
the
frequency
of
testing
for
that
facility.

NOTE:
Based
on
these
requirements,
SRT
data
are
considered
applicable
to
a
compliance
test
when
the
SRT
test
results
are
acceptable
and
the
SRT
test
is
conducted
within
30
days
of
the
compliance
test,
for
the
test
species
and
SRT
in
question.
Therefore,
it
is
not
necessary
for
an
approved
laboratory
to
run
an
SRT
test
every
month
if
the
laboratory
is
not
conducting
compliance
tests
for
a
particular
species.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
19
of
23
pages
C.
CHANGING
OF
AN
ESTABLISHED
REFERENCE
TOXICANT
The
SRT
used
for
any
species
by
a
laboratory
may
be
changed
at
any
time
provided
that
the
following
conditions
have
been
satisfied:

1.
A
series
of
at
least
three
reference
toxicant
tests
are
conducted
with
the
new
reference
toxicant
and
the
results
of
those
tests
are
identified
as
satisfactory,
in
writing,
by
the
Department.

2.
Laboratories
must
continue
using
the
already
approved
SRT
in
their
ongoing
QA/
QC
program,
until
such
time
as
the
letter
referenced
above,
is
received
by
the
laboratory.

D.
CONTROL
CHARTS
Control
charts
shall
be
established
from
SRT
test
results
in
accordance
with
the
procedures
outlined
in
the
USEPA
methods
documents.
Control
charts
shall
be
constructed
using
IC25'
s
using
the
following
methods:

1.
The
upper
and
lower
control
limits
shall
be
calculated
by
determining
+/­
two
standard
deviations
above
and
below
the
mean.

2.
SRT
test
results
which
exhibit
an
IC25
that
is
greater
than
the
highest
concentration
tested
or
less
than
the
lowest
concentration
tested
(
i.
e.
a
definitive
endpoint
cannot
be
determined),
shall
not
be
used
to
establish
control
charts.

3.
SRT
tests
which
do
not
meet
the
acceptability
criteria
for
a
specific
species
shall
not
be
used
to
establish
control
charts.

4.
All
values
used
in
the
control
charts
should
be
as
nominal
concentrations.
However,
the
control
charts
shall
be
accompanied
by
a
chart
tabulating
the
test
results
as
measured
concentrations.

5.
An
outlier
(
i.
e.
values
which
fall
outside
the
upper
and
lower
control
limits)
should
be
included
on
the
control
chart
unless
it
is
determined
that
the
outlier
was
caused
by
factors
not
directly
related
to
the
test
organisms
(
e.
g.,
test
concentration
preparation)
as
the
source
of
variability
would
not
be
directly
applicable
to
effluent
tests.
In
such
case,
the
result
and
explanation
shall
be
reported
to
the
Department
within
30
days
of
the
completion
of
the
SRT
test.

The
control
chart
established
for
the
initial
series
of
SRT
data
submitted
will
be
used
by
the
laboratory
and
the
Department
to
determine
outliers
from
SRT
test
results
reported
in
the
"
NJPDES
Biomonitoring
Report
Form
­
Chronic
Toxicity
Test"
submitted
by
the
permittees
for
the
test
species.
These
initial
control
limits
will
remain
unchanged
until
twenty
SRT
tests
have
been
completed
by
the
laboratory.

The
following
procedures
shall
be
used
for
continually
updating
control
charts
after
twenty
acceptable
SRT
tests
have
been
completed:

1.
Once
a
laboratory
has
completed
twenty
acceptable
SRT
tests
for
a
test
species,
the
upper
and
lower
control
limits
shall
be
recalculated
with
those
twenty
values.

2.
For
each
successive
SRT
test
conducted
after
these
first
twenty
tests,
a
moving
average
shall
be
calculated
and
the
control
limits
reevaluated
using
the
last
twenty
consecutive
test
results.

3.
The
upper
and
lower
control
limits
shall
be
reported
on
the
"
NJPDES
Biomonitoring
Report
Form
­
Chronic
Toxicity
Tests"
along
with
the
SRT
test
result.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
20
of
23
pages
E.
UNACCEPTABLE
SRT
TEST
RESULTS
If
a
laboratory
produces
any
SRT
test
results
which
are
outside
the
established
upper
and
lower
control
limits
for
a
test
species
at
a
frequency
greater
than
one
test
in
any
ten
tests,
a
report
shall
be
forwarded
to
the
Department
at
the
address
contained
herein.
This
report
shall
include
any
identified
problem
which
caused
the
values
to
fall
outside
the
expected
range
and
the
corresponding
actions
that
have
been
taken
by
the
laboratory.
The
Department
may
not
accept
or
may
require
repeat
testing
for
any
toxicity
testing
that
may
have
been
affected
by
such
an
occurrence.

If
a
laboratory
produces
two
consecutive
SRT
test
results
or
three
out
of
any
ten
test
results
which
are
outside
the
established
upper
and
lower
limits
for
a
specific
test
species,
the
laboratory
shall
be
unapproved
to
conduct
chronic
toxicity
tests
for
compliance
purposes
for
that
test
species.
Reapproval
shall
be
contingent
upon
the
laboratory
producing
SRT
test
results
within
the
established
upper
and
lower
control
limits
for
that
test
species
in
two
consecutive
SRT
tests.
If
one
or
both
of
those
test
results
again
fall
outside
the
established
control
levels,
the
laboratory
is
unapproved
for
that
test
species
until
five
consecutive
test
results
within
the
established
upper
and
lower
control
limits
are
submitted
and
approved
by
the
Department.

F.
ANNUAL
SUBMITTALS
Control
charts
shall
be
forwarded
to
the
Department
on
an
annual
basis,
on
the
anniversary
of
approval
for
the
test
species.

The
Department
may
request,
at
any
time,
any
information
which
is
essential
in
the
evaluation
of
SRT
results
and/
or
compliance
data.
Appendix
A
­
Chronic
Toxicity
Specifications
Page
21
of
23
pages
V.
TEST
CANCELLATION
/
RESCHEDULING
EVENTS
A
lab
may
become
aware
of
QA
problems
during
or
immediately
following
a
test
that
will
prevent
data
from
being
submitted
or
a
lab
may
be
unable
to
complete
a
tests
due
to
sample
collection
or
shipping
problems.
If
for
any
reason
a
chronic
toxicity
test
is
initiated
and
then
prematurely
ended
by
the
laboratory
or
at
the
request
of
the
permittee,
the
laboratory
shall
submit
the
form
entitled
"
Chronic
Whole
Effluent
Toxicity
Testing
Test
Cancellation
/
Rescheduling
Event
Form"
contained
herein.
This
form
shall
be
used
to
detail
the
reason
for
prematurely
ending
the
test.
This
completed
form
and
any
applicable
raw
data
sheets
shall
be
submitted
to
the
appropriate
biomonitoring
program
at
the
address
above
within
30
days
of
the
cessation
of
the
test.

Tests
are
considered
to
be
initiated
once
test
organisms
have
been
added
to
all
test
chambers.

Submission
of
this
form
does
not
relieve
the
facility
from
monitoring
for
that
monitoring
period.

VI.
REPORTING
The
report
form
entitled
"
NJPDES
Biomonitoring
Report
Form
­
Chronic
Toxicity
Tests"
should
be
used
to
report
the
results
of
all
NJPDES
chronic
compliance
biomonitoring
tests.
Laboratory
facsimiles
are
acceptable
but
must
contain
all
information
included
on
any
recent
revisions
of
the
form
by
the
Department.
Statistical
printouts
and
raw
data
sheets
for
all
endpoints
analyzed
shall
be
included
with
the
report
submitted
to
the
Department.
Two
copies
of
all
chronic
toxicity
test
report
forms
shall
be
submitted
to
the
following
address
as
applicable:

Bureau
of
Point
Source
Permitting
Region
1
OR
Bureau
of
Point
Source
Permitting
Region
2
(
as
indicated
in
the
cover
letter)

New
Jersey
Department
of
Environmental
Protection
Division
of
Water
Quality
PO
Box
29
Trenton,
NJ
08625­
0029
It
is
not
necessary
to
attach
a
copy
of
a
test
report
form
to
the
Discharge
Monitoring
Report
(
DMR)
form
when
submitting
this
form
to
the
Department.
However,
the
results
of
all
chronic
toxicity
tests
conducted
for
compliance
purposes
must
be
reported
on
the
DMR
form
under
the
appropriate
parameter
code
in
the
monitoring
period
in
which
the
test
was
conducted.

VII.
METHOD
SPECIFICATIONS
The
following
method
specifications
shall
be
followed
as
specified
in
the
NJPDES
permit.
Any
changes
to
these
methods
will
not
be
considered
acceptable
unless
they
are
approved
in
writing
by
the
Department,
prior
to
their
use.

A.
Fathead
Minnow
(
Pimephales
promelas),
Larval
Survival
and
Growth
Test,
method
1000.0
B.
Ceriodaphnia
dubia,
Survival
and
Reproduction
Test,
method
1002.0
C.
Algal,
(
Selenastrum
capricornutum),
Growth
Test,
method
1003.0
D.
Sheepshead
Minnow
(
Cyprinodon
variegatus),
Larval
Survival
and
Growth
Test,
method
1005.0
E.
Inland
Silverside
(
Menidia
beryllina),
Larval
Survival
and
Growth
Test,
method
1006.0
F.
Mysidopsis
bahia,
Survival,
Growth,
and
Fecundity
Test,
method
1007.0
G.
Champia
parvula,
Sexual
Reproduction
Test,
method
1009.0
Appendix
A
­
Chronic
Toxicity
Specifications
Page
22
of
23
pages
VIII.
REFERENCES
1.
Keating,
K.
1985.
The
influence
of
Vitamin
B12
deficiency
on
the
reproduction
of
Daphnia
pulex
Leydig
(
Cladocera).
J.
Crustacean
Biology
5:
130­
136.

2.
Keating,
K.
1988.
N.
J.
D.
E.
P.
Project
C29589,
Fiscal
1988
Third
Quarter
Summary
Report.
Producing
Nutritionally
Competent
Daphnids
for
Use
in
Bioassay.
44p.

3.
Keating,
K.,
and
B.
Dagbusan.
1984.
Effect
of
selenium
deficiency
on
cuticle
integrity
in
Cladocera
(
Crustacea).
Proc.
Natl.
Acad.
Sci.
USA
81:
3433­
3437.

4.
NJDEP,
1993.
Discharge
Monitoring
Report
(
DMR)
Instruction
Manual.

5.
USEPA.
1994.
Short
Term
Methods
for
Estimating
the
Chronic
Toxicity
of
Effluents
and
Receiving
Waters
to
Marine
and
Estuarine
Organisms.
EPA­
600/
4­
91­
003.
July
1994.
Second
Edition.

6.
USEPA.
1994.
Short
Term
Methods
for
Estimating
the
Chronic
Toxicity
of
Effluents
and
Receiving
Waters
to
Freshwater
Organisms.
EPA/
600/
4­
91/
002.
July
1994.
Third
Edition.
toc_
dsw.
rtf
NEW
JERSEY
DEPARTMENT
OF
ENVIRONMENTAL
PROTECTION
PO
Box
29
TRENTON,
NEW
JERSEY
08625­
0029
BIOMONITORING
PROGRAM
CHRONIC
WHOLE
EFFLUENT
TOXICITY
TESTING
TEST
CANCELLATION
/
RESCHEDULING
EVENT
FORM
NJPDES
No.:
________________________

FACILITY
NAME:
__________________________________________________________________________

LOCATION:
__________________________________________________________________________

CONTACT:
_________________________________________
PHONE:
________________________

CANCELLATION
EVENT:

EFFLUENT
SAMPLING:

(
ALL
APPLICABLE
RAW
DATA
SHEETS
MUST
BE
ATTACHED)
c:
Permittees
authorized
agent.
THIS
FORM
IS
TO
BE
COMPLETED
AND
SUBMITTED
TO
THE
DEPARTMENT
DIRECTLY
BY
THE
LABORATORY
CONDUCTING
CHRONIC
TOXICITY
TESTS
WHENEVER
A
CHRONIC
TOXICITY
TEST
IS
PREMATURELY
ENDED
FOR
ANY
REASON
LABORATORY
NAME
/
NUMBER:
___________________________________________________________

CONTACT:
___________________________________________________________

TEST
START
DATE:
_____/______/______
TEST
END
DATE:
_____/_____/_____

REASON
FOR
CANCELLATION:
_________________________________________________________________

________________________________________________________________________________________________

________________________________________________________________________________________________

________________________________________________________________________________________________

________________________________________________________________________________________________

________________________________________________________________________________________________

SAMPLING
POINT
/
DESCRIPTION
OF
SAMPLING
SITE:
___________________________________________

________________________________________________________________________________________________

SAMPLING
INITIATED:
DATE:
____/____/____
TIME:
___________

SAMPLING
ENDED:
DATE:
____/____/____
TIME:
___________

NUMBER
OF
EFFLUENT
SAMPLES
COLLECTED:
____________________

SAMPLE
TYPE
(
GRAB/
COMPOSITE):
___________________________________

RECEIVED
IN
LAB
BY/
FROM:
__________________________________________________________________

_______________________________________________________________________________________________

METHOD
OF
SHIPMENT:
_______________________________________________________________________
toc_
dsw.
rtf
