EXAMPLE
PROCEDURE
FOR
COLLECTING
BULK
WATER
SAMPLES
FOR
LABORATORY
FILTRATION
AND
CRYPTOSPORIDIUM
ANALYSIS
JUNE
2003
DRAFT
Draft
June
2003
1
Example
Procedure
for
Collecting
Bulk
Water
Samples
for
Laboratory
Filtration
and
Cryptospordium
Analysis
1.0
Required
Materials
Check
to
make
sure
the
following
materials
are
available
before
collecting
sample:

Q
Several
pairs
of
new,
powder­
free
latex
gloves
(
Lab
Safety
Supply,
cat.
number
16285XL,
or
equivalent)

Q
Sample
collection
form
Q
10­
L
cubitainer
(
Eagle
Picher
cat.
number
360­
2.5,
or
equivalent)

Q
Sample
number
label
9
Waterproof
pen
Q
Cooler,
approximately
34­
quart
(
Coleman,
model
number
5281A703,
or
equivalent)

Q
Two
large
plastic
trash
bags
Q
Two
8­
lb.
bags
of
ice
Q
Four
13
×
18
inch
ziplock
bags
Q
Strapping
tape
Q
Two,
self­
adhesive
plastic
airbill
sleeves
Q
Airbill
for
shipment
2.0
Collecting
the
Sample
2.1
Put
on
a
pair
of
powder­
free
latex
gloves.

2.2
Turn
on
the
influent
tap
and
flush
the
system
by
allowing
source
water
to
flow
for
2
to
3
minutes
or
until
any
debris
that
has
accumulated
has
cleared
or
the
turbidity
in
the
water
becomes
visibly
uniform.

2.3
While
the
system
is
flushing,
record
the
following
information
on
the
sample
collection
form:

O
Public
water
system
(
PWS)
name
O
PWS
address
O
Sampler
name
O
Sample
ID
(
optional)

O
Public
Water
System
Identification
(
PWS
ID)
number
O
Public
Water
System
facility
ID
number
O
Facility
name
O
Sample
collection
point
ID
O
Sample
collection
point
name
O
Sample
collection
date
Draft
June
2003
2
O
Source
water
type
(
optional
[
but
required
for
E.
coli
sample
forms])

O
Requested
analysis
(
circle
Cryptosporidium
field
sample
for
routine
monitoring
sample;
circle
both
"
Cryptosporidium
field
sample"
and
"
Cryptosporidium
matrix
spike"
sample
if
you
are
sending
an
additional
sample
with
the
monitoring
sample
for
matrix
spike
analysis)

2.4
After
the
system
has
been
flushed,
any
optional
water
quality
parameters
such
as
temperature,
pH,
and/
or
turbidity
should
be
measured
now.

2.5
Fill
the
10­
L
cubitainer.
If
both
a
field
sample
and
a
matrix
spike
sample
are
being
collected,
fill
one
immediately
after
the
other.
Record
the
following
information
on
the
sample
collection
form:

O
Sample
collection
time
O
Comments
to
laboratory,
if
needed
2.6
Immediately
following
sample
collection,
tighten
the
cubitainer
cap(
s)
and
place
the
cubitainer(
s)
in
a
refrigerator
to
chill
prior
to
packing
the
shipping
cooler
for
shipment.
If
no
refrigerator
is
available,
and
the
sample
will
not
be
shipped
for
several
hours,
place
the
cubitainer(
s)
in
the
shipping
cooler
with
ice
to
chill,
and
replace
the
ice
before
shipping.

Note!
Method
1622/
1623
requires
that
the
temperature
of
the
sample
upon
arrival
at
the
laboratory
must
be
<
10
°
C
(
but
not
frozen),
and
the
laboratory
must
have
the
filter
processed
within
96
hours
of
sample
collection.
If
the
sample
temperature
and
holding
time
requirements
are
not
met,
then
the
sample
is
invalid
and
must
be
recollected.

3.0
Packing
the
Sample
3.1
Insert
two
large
plastic
trash
bags
into
the
shipping
cooler
to
create
a
double
liner.
Immediately
before
packing
the
cooler,
place
an
8­
lb
bag
of
ice
into
each
of
the
two
plastic,
ziplock
bags.
To
prevent
leaks
place
each
ice
pack
into
an
additional
ziplock
bag.
Seal
each
ziplock
bag,
expelling
as
much
air
as
possible,
and
secure
top
with
tape.

Note!
Shipping
companies
may
delay
sample
shipments
if
leakage
occurs.
Double
liners
and
ziplock
bags
around
ice
will
prevent
leakage
and
delays.

3.2
Place
the
chilled
cubitainer
upright
into
the
center
of
the
lined
cooler.
Place
the
two
ice
packs
into
the
cooler,
one
on
each
side
of
the
cubitainer.

3.3
If
you
will
be
monitoring
sample
temperature
during
shipment,
place
in
the
cooler
the
temperature
monitoring
device
(
e.
g.,
extra
sample
bottle
for
measuring
sample
temperature
upon
receipt
at
the
laboratory,
thermometer
vial,
or
Thermochron
 
iButton).
Seal
each
liner
bag
by
twisting
top
of
bag
and
tying
in
a
knot.

3.4
Peel
the
backing
off
one
of
the
plastic
airbill
sleeves
and
attach
the
sleeve
to
the
inside
of
the
cooler
lid.

O
Sign
and
date
the
sample
collection
form.

Fold
the
completed
sample
collection
form,
and
place
it
inside
the
plastic
sleeve.
Draft
June
2003
3
3.5
Close
the
cooler
lid,
seal
the
horizontal
joints
with
duct
tape,
and
secure
the
lid
with
tape
by
taping
the
cooler
at
each
end,
perpendicular
to
the
seal.

Note!
Shipping
companies
may
delay
sample
shipments
if
leakage
occurs.
Be
sure
to
seal
the
cooler
joints.

3.6
Peel
the
backing
off
of
the
second
airbill
sleeve
and
attach
the
sleeve
to
the
outside
of
the
cooler
lid.
Complete
the
shipping
airbill
with
the
laboratory
address,
billing
information,
sample
weight,
and
shipping
service.
Remove
the
shipper's
copy
of
the
airbill,
and
place
the
remainder
of
the
airbill
inside
the
plastic
sleeve.

4.0
Shipping
and
Tracking
4.1
Ship
samples
on
the
day
of
collection
and
use
a
reliable
shipping
service
for
next­
day
delivery.
If
samples
are
not
shipped
the
day
of
collection,
the
sample
must
be
maintained
at
<
10
°
C
(
but
not
frozen)
by
chilling
in
a
refrigerator
or
cooler
filled
with
ice.

4.2
Contact
the
laboratory
to
notify
them
of
the
sample
shipment.
Request
that
the
laboratory
contact
you
the
next
day
if
the
sample
is
not
received.

4.3
Using
the
airbill
number
on
the
shipper's
copy
of
the
airbill,
track
the
sample
shipment
using
the
shipping
company's
web
page
or
by
contacting
the
shipping
company
over
the
phone.

4.4
If
problems
are
encountered
with
the
shipment,
communicate
with
the
shipping
company
to
resolve,
and
update
the
laboratory
regarding
the
status
of
the
shipment.
