PAGE
INTENTIONALLY
LEFT
BLANK
William
C.
Herz
Director
of
Scientific
Programs
M
E
M
O
R
A
N
D
U
M
TO:
Product
Testing
Recipient
FROM:
William
C.
Herz,
Director,
Scientific
Programs
SUBJECT:
UAN
Solutions
Product
Testing
Dossier
DATE:
March
15,
2003
It
is
with
great
pleasure
that
The
Fertilizer
Institute
(
TFI)
announces
the
completion
and
distribution
of
the
final
product
testing
dossier
for
UAN
Solutions
(
CAS
#
15978­
77­
5).

As
you
are
aware,
TFI
sponsored
this
four
 
year
program
to
develop
and
summarize
screening­
level
hazard
information
for
high
production
volume
(
HPV)
chemicals.
The
data
elements
generated
represent
a
broad
overview
of
human
health
and
ecological
parameters.
These
include
a
physical
­
chemical
characterization,
environmental
fate,
mammalian
toxicity
and
ecotoxicity.
A
health
and
environmental
safety
data
summary
dossier
was
prepared
for
each
of
the
23
materials.
It
summarizes
the
available
literature
data,
new
testing
data,
category
description
and
read
across
data
as
well
as
provides
a
conclusion
regarding
the
inherent
hazards
of
the
material.

Upon
receipt
of
this
data
a
90
calendar
day
regulatory
trigger
starts
within
which
you
must
update
your
material
safety
data
sheets
(
MS­
DS).
Upon
first
product
shipment
you
must
also
notify
your
distributors
and
employers
once
the
MS­
DS
has
been
updated.
These
regulatory
requirements
are
detailed
in
29
CFR
1910.1200(
g)(
5)
and
29
CFR
1910.1200(
g)(
6)(
i).
The
requirements
are
found
in
the
Occupational
Safety
and
Health
Administration
"
Hazard
Communication
Standard"
(
29
CFR
1910.1200).

Please
note
that
by
receipt
of
this
data;
you
agree
not
to
distribute
or
sell
this
data
beyond
your
own
company.
PAGE
INTENTIONALLY
LEFT
BLANK
HEALTH
&
ENVIRONMENTAL
SAFETY
DATA
SUMMARY
DOCUMENT
NITROGEN
SOLUTIONS
(
UAN)
CAS
#
15978­
77­
5
Prepared
for:

THE
FERTILIZER
INSTITUTE
January
27,
2003
THE
WEINBERG
GROUP
INC.
1220
Nineteenth
St,
NW,
Suite
300
Washington,
DC
20036­
2400
e­
mail
science@
weinberggroup.
com
WASHINGTON
NEW
YORK
SAN
FRANCISCO
BRUSSELS
PARIS
PAGE
INTENTIONALLY
LEFT
BLANK
TABLE
OF
CONTENTS
Page
EXECUTIVE
OVERVIEW..................................................................................................
1
SIDS
DATA
PROFILE.........................................................................................................
7
SIDS
DATA
SUMMARY....................................................................................................
8
1.
GENERAL
INFORMATION...................................................................................
10
2.
PHYSICAL­
CHEMICAL
DATA.............................................................................
13
3.
ENVIRONMENTAL
FATE
AND
PATHWAYS....................................................
15
4.
ECOTOXICITY........................................................................................................
17
5.
TOXICITY................................................................................................................
20
6.
REFERENCES..........................................................................................................
23
APPENDICES
Appendix
A
SIDS
Data
Availability
Summary
Appendix
B
SIDS
Data
Summaries
for
the
Nitrate
Compounds
Category:
Ammonium
Nitrate,
Nitrogen
Solutions
(
primarily
Urea
Ammonia
Nitrogen
[
UAN]),
Potassium
Nitrate,
Potassium
Sodium
Nitrate,
and
Sodium
Nitrate
ACRONYMS
AND
ABBREVIATIONS
BCF
Bioconcentration
Factor
bw
Body
Weight
DAP
Diammonium
Phosphate
DOT
Department
of
Transportation
FDA
U.
S.
Food
and
Drug
Administration
g/
L
Grams
per
Liter
GLP
Good
Laboratory
Practices
GTSP
Granular
Triple
Super
Phosphate
g/
mL
Grams
per
Milliliter
HSDB
Hazardous
Substance
Data
Bank
IPCS
International
Programme
for
Chemical
Safety
KNO3
Potassium
Nitrate
Koc
Organic
Carbon
Partition
Coefficient
Kow
Octanol/
Water
Partition
Coefficient
LC50
Median
Lethal
Concentration
LD50
Median
Lethal
Dose
LOAEL
Lowest
Observable
Adverse
Effect
Level
LOEC
Lowest
Observable
Effect
Concentration
LOEL
Lowest
Observable
Effect
Level
M
Molar
MAP
Monoammonium
Phosphate
mg/
kg
Milligrams
per
Kilogram
mg/
kg/
day
Milligrams
per
Kilogram
per
Day
mg/
L
Milligrams
per
Liter
MHb
Methyl
Hemoglobin
mm
Hg
Millimeters
of
Mercury
mmol/
L
Millimoles
per
Liter
NaNo3
Sodium
Nitrate
NFPA
National
Fire
Prevention
Association
(
NH4
)
2
NO3
Ammonium
Nitrate
NIOSH
National
Institute
for
Occupational
Safety
and
Health
NO3
Nitrate
NOAEL
No
Observable
Adverse
Effect
Level
NOEC
No
Observable
Effect
Concentration
NOEL
No
Observable
Effect
Level
OECD
Organisation
for
Economic
Co­
operation
and
Development
Pa
Pascal
ppm
Parts
per
Million
SCAS
Semi
Continuous
Activated
Sludge
SIDS
Screening
Information
Data
Set
SSP
Single
Super
Phosphate
TLm
Median
Toxicity
Level
TLV
Threshold
Limit
Value
TFI
The
Fertilizer
Institute
UAN
Urea
Ammonia
Nitrogen
EXECUTIVE
OVERVIEW
I.
Introduction
The
Fertilizer
Institute,
on
behalf
of
its
member
companies,
initiated
a
Product
Testing
Project
to
collect,
review,
summarize,
and
where
necessary
develop
additional
health
and
environmental
safety
data
for
23
of
its
high
production
volume
inorganic
fertilizer
materials.
These
data
and
summaries
provide
valuable
information
that
can
be
used
to
update
Material
Safety
Data
Sheets,
answer
customers'
questions,
and
support
product
stewardship
efforts.
The
chemical
industry
is
also
participating
in
a
voluntary
program
of
comparable
scope
for
high
production
volume
organic
chemicals.
1
The
23
fertilizer
materials
were
divided
into
five
categories
(
i.
e.,
ammonia
compounds,
nitrate
compounds,
phosphate
compounds,
salts
and
acids)
based
on
their
primary
constituents
as
shown
in
Table
1.
The
use
of
categories
is
a
recognized
and
accepted
method
that
allows
health
and
environmental
safety
data
from
one
chemical
in
the
category
to
be
used
to
represent
one
or
more
other
related
chemicals
in
the
category
(
USEPA
1999).
The
key
is
to
find
similar,
or
at
least
predictable,
patterns
and
trends
among
the
chemicals
in
a
category.
In
this
way,
data
can
be
pooled,
resources
are
optimized,
and
fewer
animals
are
used
in
testing,
all
without
losing
the
ability
to
evaluate
the
hazards
and
safety
of
the
individual
chemicals.
Note
that
some
of
the
materials
fall
into
more
than
one
category
(
e.
g.,
diammonium
phosphate
[
DAP]
is
in
both
the
phosphate
and
ammonia
categories).

Searches
were
conducted
using
on­
line
databases,
standard
reference
texts,
and
other
published
sources
for
toxicity,
ecotoxicity,
environmental
fate,
and
physical­
chemical
properties.
The
collected
data
were
reviewed
for
quality
and
acceptability
and
then
summarized
according
to
the
Organization
for
Economic
Cooperation
and
Development
(
OECD)
Screening
Information
Data
Set
(
SIDS)
dossier
format
(
OECD
1997).
The
OECD
countries
(
including
the
United
States)
have
agreed
on
a
set
of
tests
and
on
types
of
data
that
are
generally
necessary
to
characterize
the
chemical
behavior
and
potential
hazards
of
chemicals
released
into
the
environment.
The
OECD
SIDS
dossier
was
chosen
as
a
standard
format
for
the
TFI
Product
Testing
Project
in
order
that
it
would
be
scientifically
defensible,
broadly
applicable
and
easily
understandable
to
a
wide
range
of
stakeholders.

The
following
sections
of
this
Executive
Overview
provide
the
rationale
for
development
of
the
Nitrate
Compounds
category
(
Section
II);
a
synopsis
of
the
available
data
related
to
the
physical­
chemical
properties,
environmental
fate,
ecotoxicity
and
toxicity
of
the
nitrate
compounds
in
this
category
(
Section
III);
and
a
conclusion
regarding
the
adequacy
of
the
data
to
sufficiently
characterize
the
category
and
the
need
for
additional
testing
(
Section
IV).

The
data
for
nitrogen
solutions
(
UAN)
are
summarized
in
the
Chemical
Profile
and
Data
Summary
tables.
Because
the
evaluation
of
data
adequacy
relies
on
a
category
approach,
the
Data
Summary
table
references
Appendix
B
when
data
are
available
for
one
or
more
of
the
other
materials
in
the
category
for
those
data
elements
where
no
nitrogen
solutions
(
UAN)
data
are
1
HPV
Chemical
Challenge
Program;
USEPA
1999
(
http://
www.
epa.
gov/
opptintr/
chemrtk/
volchall.
htm)

1
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
available.
Appendix
B
provides
a
summary
of
the
data
for
all
of
the
nitrate
compounds
in
the
category.
The
individual
studies
for
nitrogen
solutions
(
UAN)
itself
are
presented
in
subsequent
pages
of
this
document.
Separate
data
summary
documents
are
available
for
each
of
the
nitrate
compounds
in
the
category.

II.
Rationale
for
the
Nitrate
Compounds
Category
The
nitrate
compounds
category
for
fertilizer
materials
includes
ammonium
nitrate,
nitrogen
solutions
(
primarily
Urea
Ammonia
Nitrogen
[
UAN]),
potassium
nitrate,
sodium
nitrate,
and
potassium
sodium
nitrate.
These
compounds
are
structurally
similar.
In
solution
these
compounds
all
dissociate
into
a
common
nitrate
ion:

O
N'

O
O
To
this
base,
the
compounds
vary
according
to
the
simple
cation
present
(
i.
e.,
K+
,
Na+
,
NH4
+
).
The
six
compounds
have
similar
and
generally
predictable
patterns
of
behavior
in
the
environment
as
well
as
toxicological
properties.
Nitrate
itself
is
less
toxicologically
important
than
is
nitrite.
Nitrate
is
reduced
to
nitrite
by
the
enzyme
nitrate
reductase
which
is
found
in
plants,
certain
bacterial
species,
and
mammalian
gastric
tissues
(
Owens
and
Dubeski
1989).
After
ingestion
nitrates
are
reduced
to
nitrites
by
bacteria
in
the
lower
intestine
of
the
adult
(
L'hirondel
and
L'hirondel
1998).
In
babies,
it
is
this
nitrite
that
can
lead
to
a
condition
called
methemoglobinemia
("
blue
baby
syndrome")
(
Burden
1961).

In
addition
to
methemoglobinemia,
some
evidence
indicates
a
possible
formation
of
N­
nitroso
compounds
in
foods
and
in
tissues
that
contain
excess
nitrates/
nitrites
(
Chow
et
al.
1980).
N­
nitroso
compounds
(
e.
g.,
nitrosamines)
are
known
to
be
potent
mutagens/
carcinogens
(
Lijinksy
and
Epstein
1970;
Magee
and
Barnes
1967).
However,
the
quantities
of
nitrosamines
formed
in
mammals
from
nitrates
in
food
are
negligible
and
all
of
the
epidemiological
studies
published
since
1985
concluded
that
there
is
no
positive
correlation
between
nitrate
intakes
and
the
incidence
of
cancer
(
Al­
Dabbagh
et
al.
1986;
Forman
et
al.
1985;
Leclerc
et
al.
1991;
Lijinsky
1989;
Lijinksy
and
Epstein
1970;
Magee
and
Barnes
1967).
In
fact,
some
evidence
suggests
that
nitrates
in
foods
may
actually
play
an
anti­
carcinogenic
role
(
Lijinsky
1989).

In
aquatic
systems,
the
toxicity
of
the
various
nitrate
materials
is
at
least
partially
related
to
the
cationic
composition
of
the
solution,
although
the
available
data
suggest
that
aquatic
toxicity
is
not
a
major
concern
(
Rubin
and
Elmaraghy
1977).

In
terrestrial
systems,
nitrate
absorbed
by
plants
is
reduced
to
nitrite
by
the
enzyme
nitrate
reductase
(
Owens
and
Dubeski
1989).
The
nitrite
will
be
further
reduced
only
when
photosynthesis
and
carbohydrate
synthesis
are
active
(
Owens
and
Dubeski
1989).
Under
drought
conditions,
frost
and
shading,
or
deficiencies
of
other
nutrients,
each
of
which
reduce
photosynthesis
and
biosynthesis
of
protein,
nitrate
continues
to
be
absorbed
and
may
accumulate
in
plant
tissue
(
Owens
and
Dubeski
1989).
Under
most
conditions,
however,
the
nitrate
ion
will
2
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
leach
into
the
soil
pore
water
and
become
indistinguishable
from
the
natural
nitrate
already
present.

III.
Summary
of
Data
Available
for
the
Nitrate
Compounds
Category
Physical­
Chemical
Data
The
density
of
nitrate
compounds
varies
from
0.75­
2.109
g/
mL.
All
nitrate
compounds
are
highly
soluble
in
water
with
solubility
ranging
from
357­
1,920
g/
L
at
20­
25
°
C.
Although
few
vapor
pressure
values
are
available,
based
on
the
fact
that
these
compounds
are
solids,
it
may
be
concluded
that
they
have
low
volatility.
Very
low
octanol/
water
partitioning
coefficients
indicate
a
low
bioaccumulation
potential.

Environmental
Fate
and
Pathway
Nitrate
compounds
biodegrade
in
the
environment
under
certain
conditions.
Their
high
solubility
and
expected
low
volatility
suggest
that
they
are
present
mostly
in
aqueous
solutions.
While
the
cation
may
bind
to
clay
particles
in
the
soil,
the
nitrate
anion
may
show
significant
leaching
to
ground
water.
Nitrate
is
subject
to
plant
uptake
and
loss
from
the
ecosystem
in
runoff
waters
or
by
denitrification.

Ecotoxicity
The
acute
96­
hour
LC50
values
for
fish
range
from
327
mg
NO3
/
L
to
greater
than
9,300
mg
NO3
/
L.
In
addition,
acute
fish
toxicity
studies
were
conducted
on
nitrogen
solutions
(
UAN)
and
potassium
sodium
nitrate
under
Good
Laboratory
Practice
(
GLP)
conditions.
The
resultant
LC50
values
were
greater
than
approximately
100
mg/
L,
the
highest
concentrations
tested.
Acute
studies
with
Daphnia
magna
resulted
in
EC50
values
ranging
from
300
to
2,614
mg
NO3
/
L.
Chronic
toxicity
studies
on
aquatic
invertebrates
resulted
in
NOEC
values
of
300
mg/
L
and
TLm
values
of
327­
880
mg
NO3
/
L.
Algal
toxicity
studies
resulted
in
EC50
values
from
83
to
1,233
mg
NO3
/
L.
The
available
ecotoxicity
data
indicate
nitrate
compounds
have
relatively
low
toxicity
to
terrestrial
plants.
Based
on
the
standard
Federal
Insecticide
Fungicide
and
Rodenticide
Act
(
FIFRA)
acute
toxicity
ratings
for
fish
and
Daphnia
(
below),
the
compounds
in
this
category
are
considered
practically
non­
toxic.

EC/
LC50
(
mg/
L)
Toxicity
Description
<
0.1
Very
Highly
Toxic
0.1­
1
Highly
Toxic
1­
10
Moderately
Toxic
10­
100
Slightly
Toxic
>
100
Practically
Non­
Toxic
3
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
Mammalian
Toxicity
Acute
toxicity
The
acute
oral
LD50
values
for
mammals
were
up
to
5,200
mg/
kg
bw
for
most
of
the
nitrate
compounds.
In
addition,
acute
oral
toxicity
studies
were
conducted
under
GLP
conditions
for
UAN
and
potassium
sodium
nitrate.
Results
of
the
acute
oral
toxicity
studies
for
both
materials
confirmed
that
they
were
of
low
toxicity
concern
(
LD50
values
>
2000
mg/
kg).
An
acute
inhalation
study
using
ammonium
nitrate
reported
an
LC50
of
>
88.8
mg/
L,
while
a
similar
study
with
dogs
reported
an
NOEC
of
5
mg
NO3
/
m3
(
the
only
dose
tested).
Acute
dermal
studies
were
also
conducted
under
GLP
conditions.
The
resultant
LD50
s
were
>
5000
mg/
kg
bw
for
both
ammonium
nitrate
and
potassium
nitrate.
Based
on
the
standard
FIFRA
acute
toxicity
ratings
for
mammals
(
below),
the
compounds
in
this
category
are
considered
to
be
of
low
to
very
low
toxicity
(
40
CFR
156.62).

Toxicity
Category
I
II
III
IV
Toxicity
High
Moderate
Low
Very
Low
Rating
500­
5000
200­
2000
mg/
kg
00­
20,0
mg/
kg
mg/
kg
Oral
LD50
 
50
mg/
kg
>
50­
500
mg/
kg
>
>
5000
mg/
kg
Dermal
LC50
 
200
mg/
kg
>
>
2000
>
20,000
mg/
kg
Inhalation
 
0.2
mg/
L
>
0.2­
2
mg/
L
>
2­
20
mg/
L
>
20
mg/
L
LC50
ses
(
7.2
mg/
mL)
in
in
vitro
chromosome
aberration
studies
with
sodium
nitrate
only.
In
vivo
assays
were
generally
negative
except
for
sodium
nitrate
at
icity
ve
toxicity,
and
developmental
toxicity.
Results
demonstrate
no
adverse
ffects
on
general
toxicity,
reproductive,
or
developmental
endpoints.
The
resultant
NOAEL
as
1,500
mg/
kg/
day.
Genotoxicity
All
nitrate
compounds
tested
negative
for
mutagenicity
using
in
vitro
bacterial
tests.
Positive
responses
were
observed
at
high
do
very
high
doses
(
706
mg
NO3/
kg).

Repeated
dose
and
developmental
toxicity
Repeat
dose
studies
on
ammonium
nitrate,
potassium
nitrate,
sodium
nitrate
indicate
that
materials
in
the
nitrate
category
are
of
low
acute
toxicity.
Developmental
or
teratogenic
tox
was
not
observed
for
any
of
the
three
nitrate
materials
for
which
data
were
available.
A
new
repeat
dose
study
on
potassium
nitrate
has
been
conducted
using
the
OECD
Guideline
422
protocol
under
GLP
conditions.
This
study
provides
data
on
three
different
endpoints;
repeat
does
toxicity,
reproducti
e
w
4
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
IV.
Conclusion
for
the
Nitrate
Compounds
Category
Substantial
literature
data
were
available
for
the
fertilizer
materials
comprising
the
nitrate
compounds
category.
The
exceptions
were
nitrogen
solutions
(
UAN)
and
potassium
sodium
nitrate
for
which
no
specific
data
were
available.
However,
UAN
is
an
aqueous
solution
of
ammonium
nitrate
and
urea
while
potassium
sodium
nitrate
is
a
simple
blend
of
potassium
nitrate
and
sodium
nitrate.
Extensive
data
are
available
for
those
base
materials
and
are
therefore
applicable
for
characterization
of
the
mixtures.
In
addition,
aquatic
and
mammalian
acute
studies
were
conducted
under
GLP
conditions
to
provide
specific
information
on
UAN
and
potassium
sodium
nitrate.
Additional
studies
were
also
conducted
under
GLP
conditions
on
ammonium
nitrate
and
potassium
nitrate
to
further
characterize
these
key
materials
in
the
category.
The
existing
data,
and
the
results
from
the
additional
studies,
adequately
characterize
the
nitrate
compounds
category.

5
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
TABLE
1:
CATEGORIES
FOR
PRODUCT
TESTING
PROJECT
CATEGORY
COMPOUND
CAS
NUMBER
Ammonia
Compounds
Anhydrous
ammonia
Aqua
ammonia
Ammonium
nitrate
Ammonium
sulfate
Ammonium
thiosulfate
Nitrogen
solutions
(
UAN)
Ammonium
phosphate
sulfate
Diammonium
phosphate
(
DAP)
Monoammonium
phosphate
(
MAP)
Urea
7664­
41­
7
1336­
21­
6
6484­
52­
2
7783­
20­
2
7783­
18­
8
15978­
77­
5
12593­
60­
1
7783­
28­
0
7722­
76­
1
57­
13­
6
Nitrate
Compounds
Sodium
nitrate
Ammonium
nitrate
Potassium
nitrate
Potassium
sodium
nitrate
Nitrogen
solutions
(
UAN)
7631­
99­
4
6484­
52­
2
7757­
79­
1
7757­
79­
1/
7631­
99­
4
15978­
77­
5
Phosphate
Compounds
Diammonium
phosphate
(
DAP)
Monoammonium
phosphate
(
MAP)
Liquid
polyphosphate
Single
superphosphate**
Granular
triple
superphosphate**
7783­
28­
0
7722­
76­
1
­­
8011­
76­
5
65996­
95­
4
Salts
Potassium
chloride
Potassium
magnesium
sulfate
Potassium
nitrate
Potassium
sodium
nitrate
Potassium
sulfate
Calcium
sulfate
7447­
40­
7
14168­
73­
1
7757­
79­
1
7757­
79­
1/
7631­
99­
4
7778­
80­
5
7778­
18­
9
Acids
Phosphoric
acid
Nitric
acid
Sulfuric
acid
7664­
38­
2
7697­
37­
2
7664­
93­
9
*
=
Nitrogen
solutions
are
primarily
Urea­
Ammonia­
Nitrogen
(
UAN;
15978­
77­
5)
**
=
Single
superphosphate
and
granular
triple
superphosphate
are
combined
into
a
single
health
and
environmental
safety
data
summary
document.
­­
=
No
CAS
number
readily
available
5
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
V.
REFERENCES
CITED
40
CFR
156.62.
Toxicity
Category.

Al­
Dabbagh,
S.,
Forman,
D.,
Bryson,
D.,
Stratton,
I.
and
Doll,
R.
1986.
Mortality
of
nitrate
fertilizer
workers.
British
Journal
of
Industrial
Medicine.
43:
507­
515.

Burden,
E.
H.
W.
J.
1961.
The
toxicology
of
nitrates
and
nitrites
with
particular
reference
to
the
potability
of
water
supplies.
The
Analyst.
86(
1024):
429­
433.

Chow,
C.
K.,
Chen,
C.
J.
and
Gairola,
C.
1980.
Effect
of
nitrate
and
nitrite
in
drinking
water
in
rats.
Toxicology
Letters.
6:
199­
206.

Forman,
D.,
Al­
Dabbagh,
S.
and
Doll,
R.
1985.
Nitrates,
nitrites
and
gastric
cancer
in
Great
Britain.
Nature.
313(
6004):
620­
625.

Leclerc,
H.,
Vincent,
P.
and
Vandevenne,
P.
1991.
Nitrates
in
drinking
water
and
cancer.
Bull.
Acad.
Natl.
Med.
175(
4):
651­
666.

L'hirondel,
J.,
L'hirondel,
J.
L.
1998.
Nitrates
and
Man:
The
Myth
of
Their
Toxicity
.

Lijinsky,
W.
and
Epstein,
S.
S.
1970.
Nitrosamines
as
environmental
carcinogens.
Nature
225(
227):
21­
23.

Lijinsky,
W.
1989.
Environmental
cancer
risks
 
real
and
unreal.
Environmental
Research
50:
207­
209.

Magee,
P.
N.
and
Barnes,
J.
M.
1967.
Carcinogenic
nitroso
compounds.
Adv.
Cancer
Res.
10:
163­
264.

OECD.
1997.
SIDS
Manual.
OECD
Secretariat,
3rd
Revision,
July
1997.

Owens,
F.
N.
and
Dubeski,
P.
1989.
Nitrate
toxicity
in
ruminants.
ASAS
Production
&
Management
Symposium.

Rubin,
A.
J.
and
Elmaraghy,
G.
A.
1977.
Studies
on
the
toxicity
of
ammonia,
nitrate
and
their
mixtures
to
guppy
fry.
Water
Research
11:
927­
935.

USEPA.
1999.
Development
of
Chemical
Categories
in
the
HPV
Challenge
Program.
www.
epa.
gove/
chemrtk/
catdoc29.
pdf.

6
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
SIDS
DATA
PROFILE
Date:
January
27,
2003
1.01
A.
CAS
No.
15978­
77­
5
1.01
C.
CHEMICAL
NAME
(
OECD
NAME)
Nitrogen
Solutions
(
UAN)

1.01
D
CAS
DESCRIPTOR
Nitrogen
Solutions
(
UAN)
1.01
G
STRUCTURAL
FORMULA
CH4
N2
O
and
H4
N2
O3
(
blend)
OTHER
CHEMICAL
IDENTITY
INFORMATION
N/
A
1.5
QUANTITY
10
million
tons
in
1999
1.7
USE
PATTERN
Primarily
liquid
fertilizer
1.9
SOURCES
AND
LEVELS
OF
EXPOSURE
Low
human
exposure
is
expected
because
UAN
is
manufactured
in
closed
systems.
Exposure
in
the
environment
is
low
because
the
product
is
a
liquid.
ISSUES
FOR
DISCUSSION
The
data
for
nitrogen
solutions
(
UAN)
should
be
evaluated
in
combination
with
the
other
materials
in
the
nitrate
category.

7
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
SIDS
DATA
SUMMARY
Date:
January
27,
2003
NITROGEN
SOLUTIONS
(
UAN)
(
CAS
NO.
15978­
77­
5)
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
See
Appendix
B
results*
2.2
Boiling
Point
See
Appendix
B
results*
2.3
Density
See
Appendix
B
results*
2.4
Vapor
Pressure
See
Appendix
B
results*
2.5
Octanol/
Water
Partition
Coefficient
See
Appendix
B
results*
2.6A
Water
Solubility
See
Appendix
B
results*
2.6B
pH
and
pKa
values
See
Appendix
B
results*
2.8
Auto
Flammability
See
Appendix
B
results*
2.9
Flammability
See
Appendix
B
results*
2.11
Oxidizing
Properties
See
Appendix
B
results*
2.12
Oxidation:
Reduction
Potential
See
Appendix
B
results*
2.13
Additional
Data
See
Appendix
B
results*
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
See
Appendix
B
results*
3.1.2
Stability
in
Water
See
Appendix
B
results*
3.1.3
Stability
in
Soil
See
Appendix
B
results*
3.3.2
Distribution
See
Appendix
B
results*
3.5
Biodegradation
See
Appendix
B
results*
3.7
Bioaccumulation
See
Appendix
B
results*
ECOTOXICITY
4.1
Acute
Toxicity
to
Fish
Oncorhynchus
mykiss
Pimephales
promelas
96
hrs
96
hrs
LC50
>
103
mg/
L
NOEC
=
103
mg/
L
LC50
between
100
and
500
mg/
L
4.2
Acute
Toxicity
to
Aquatic
Invertebrates
See
Appendix
B
results*
4.3
Toxicity
to
Aquatic
Plants
(
Algae)
See
Appendix
B
results*
4.4
Toxicity
to
Bacteria
See
Appendix
B
results*
4.5.1
Chronic
Toxicity
to
Fish
See
Appendix
B
results*
4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
See
Appendix
B
results*
4.6.1
Toxicity
to
Soil
Dwelling
Organisms
See
Appendix
B
results*
4.6.2
Toxicity
to
Terrestrial
Plants
See
Appendix
B
results*
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
See
Appendix
B
results*

TOXICITY
5.1.1
Acute
Oral
Toxicity
Rat
OECD
425
LD50
>
2000
mg/
kg/
bw
5.1.2
Acute
Inhalation
Toxicity
See
Appendix
B
results*
5.1.3
Acute
Dermal
Toxicity
See
Appendix
B
results*
5.1.4
Acute
Toxicity,
Other
Routes
See
Appendix
B
results*
5.2.1
Skin
Irritation/
Corrosion
See
Appendix
B
results*
5.2.2
Eye
Irritation
Corrosion
See
Appendix
B
results*
5.3
Skin
Sensitization
See
Appendix
B
results*
5.4
Repeated
Dose
Toxicity
See
Appendix
B
results*
5.5
Genetic
Toxicity
in
vitro
See
Appendix
B
results*
.
Gene
mutation
See
Appendix
B
results*
.
Chromosomal
aberration
See
Appendix
B
results*

*
See
Appendix
B
results
for
data
on
other
chemicals
in
the
nitrate
category.

8
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
NITROGEN
SOLUTIONS
(
UAN)
(
CAS
NO.
15978­
77­
5)
SPECIES
PROTOCOL
RESULTS
TOXICITY
(
continued)
5.6
Genetic
Toxicity
in
vivo
See
Appendix
B
results*
5.7
Carcinogenicity
See
Appendix
B
results*
5.8
Toxicity
to
Reproduction
See
Appendix
B
results*
5.9
Developmental
Toxicity/
Teratogenicity
See
Appendix
B
results*
5.11
Human
Experience
See
Appendix
B
results*
1.8
Occupational
Exposure
Limits
See
Appendix
B
results*

*
See
Appendix
B
results
for
data
on
other
chemicals
in
the
nitrate
category.

9
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
1.
GENERAL
INFORMATION
1.01
SUBSTANCE
INFORMATION
A.
CAS
number
15978­
77­
5
B.
Name
(
IUPAC
name)
Nitrogen
Solutions
(
UAN)

C.
Name
(
OECD
name)
Nitrogen
Solutions
(
UAN)

D.
CAS
Descriptor
Nitrogen
solutions
primarily
refer
to
Urea
Ammonia
Nitrate
(
UAN),
which
is
a
combination
of
urea
and
ammonium
nitrate
dissolved
in
water
to
form
a
highly
soluble
liquid
fertilizer,
typically
containing
32
parts
nitrogen.

E.
EINECS
Number
None
F.
Molecular
Formula
H8
N4
O4
C
G.
Structural
Formula
CH4
N2
O
and
H4
N2
O3
(
blend)

H.
Substance
Group
Not
applicable
I.
Substance
Remark
None
J.
Molecular
Weight
No
data
available
1.02
OECD
INFORMATION
A.
Sponsor
Country
Not
applicable
B.
Lead
Organization
Not
applicable
C.
Name
of
Responder
Name:
Mr.
William
C.
Herz,
Director
of
Scientific
Programs
Address/
Phones:
The
Fertilizer
Institute
Union
Center
Plaza
820
First
Street,
NE,
Suite
430
Washington,
DC
20002
10
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
USA
Tel:
(
202)
962­
0490
Fax:
(
202)
962­
0577
1.1
GENERAL
SUBSTANCE
INFORMATION
A.
Type
of
Substance
element
[
];
inorganic
[
X];
natural
substance
[
];
organic
[
];
organometallic
[
];
petroleum
product
[
]
Note
that
UAN
contains
urea,
which
is
considered
to
be
organic.

B.
Physical
State
(
at
20
°
C
and
1.013
hPa)

gaseous
[
];
liquid
[
X];
solid
[
]

C.
Purity
>
99%.
Solutions
sold
in
the
US
are
usually
28,
30,
or
32%
N.
Reference:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.

1.2
SYNONYMS
Nitrogen
Solutions
UAN
Urea
Ammonium
Nitrate
1.3
IMPURITIES
None
identified
1.4
ADDITIVES
None
identified
1.5
QUANTITY
Remarks:
7.9
million
tonnes
in
the
US
(
1993)
10
million
tons
in
the
US
(
1999)
References:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.
The
Fertilizer
Institute
(
TFI).
http://
www.
tfi.
org
1.6
LABELING
AND
CLASSIFICATION
No
data
available
11
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
1.7
USE
PATTERN
A.
General
Type
of
Use:
Category:

(
a)
main
Wide
dispersive
use
industrial
Agricultural
industry
use
Fertilizers
Remarks:
UAN
solutions
represent
a
large
share
of
the
liquid
fertilizer
market
and
are
gaining
popularity
worldwide.

Reference:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.

B.
Uses
in
Consumer
Products
Household
lawn
and
garden
liquid
fertilizers
1.8
OCCUPATIONAL
EXPOSURE
LIMIT
VALUE
None
established
1.9
SOURCES
OF
EXPOSURE
Remarks:
UAN
can
be
absorbed
into
the
body
by
inhalation
of
its
aerosol
and
by
ingestion,
although
significant
human
and
environmental
exposures
during
manufacture
are
unlikely
if
the
process
is
well
managed.
Engineering
controls
such
as
mechanical
ventilation,
process
or
personnel
enclosure,
control
of
process
conditions,
and
process
modifications
significantly
reduce
exposure.
Local
exhaust
(
ventilation)
control
as
close
to
the
point
of
generation
is
both
the
most
economical
and
safest
method
to
minimize
personnel
exposure
to
airborne
concentrations.
Personal
protective
equipment
includes
neoprene
or
nitrile
gloves,
dusk
masks
or
fume
hoods
as
appropriate,
chemical
safety
goggles,
and
impervious
apron,
sleeves
and
boots.
Field
exposure
to
workers
is
possible
when
handling
and
using
on
farms
as
a
fertilizer.
However,
fertilizers
are
applied
by
mechanical
spreaders,
which
reduces
contact
exposure.
Environmental
releases
are
controlled
at
the
manufacturing
plant.
Gaseous
emissions
are
low
due
to
use
of
scrubbers
and
liquid
effluent
is
recovered
as
much
as
possible.
Exposure
to
environmental
receptors
is
dependent
on
the
amount
used
as
fertilizer
on
fields,
the
climatic
conditions,
and
the
hydrological
conditions
of
12
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
the
area
of
application.
Nitrate
materials
are
common
in
the
environment
and
are
essential
nutrients
for
both
plants
and
animals.
References:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.
United
Nations
Environment
Programme
(
UNEP).
1998.
Mineral
Fertilizer
Production
and
the
Environment:
Part
1.
The
Fertilizer
Industry's
Manufacturing
Processes
and
Environmental
Issues.
In
collaboration
with
the
International
Fertilizer
Industry
Association.
Technical
Report
No.
26.

1.10
ADDITIONAL
REMARKS
Remarks:
UAN
solutions
are
usually
produced
from
urea,
ammonium
nitrate,
and
water.
Typical
input
requirements
per
tonne
of
UAN
solution
(
30%
N)
are
328
kg
urea,
426
kg
ammonium
nitrate,
245
kg
water,
and
steam/
electricity
equivalent
to
about
10
kwh.
A
corrosion
inhibitor
is
usually
added
to
protect
equipment.
References:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.
United
Nations
Environment
Programme
(
UNEP).
1998.
Mineral
Fertilizer
Production
and
the
Environment:
Part
1.
The
Fertilizer
Industry's
Manufacturing
Processes
and
Environmental
Issues.
In
collaboration
with
the
International
Fertilizer
Industry
Association.
Technical
Report
No.
26.

2.
PHYSICAL­
CHEMICAL
DATA
2.1
MELTING
POINT
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.2
BOILING
POINT
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

13
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
2.3
DENSITY
(
Relative
Density)

Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.4
VAPOR
PRESSURE
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.5
PARTITION
COEFFICIENT
logPow
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.6
WATER
SOLUBILITY
A.
Solubility
Remarks:
Highly
soluble
blend
of
urea,
ammonium
nitrate
and
water.
References:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.

B.
pH
Value
Remarks:
Adjusted
to
approximately
pH
7
References:
United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.

2.7
FLASH
POINT
(
liquids)

Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.8
AUTO
FLAMMABILITY
(
solid/
gases)

Not
applicable
14
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
2.9
FLAMMABILITY
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.10
EXPLOSIVE
PROPERTIES
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.11
OXIDIZING
PROPERTIES
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.12
OXIDATION:
REDUCTION
POTENTIAL
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

2.13
ADDITIONAL
DATA
None
3.
ENVIRONMENTAL
FATE
AND
PATHWAYS
3.1
STABILITY
3.1.1
PHOTODEGRADATION
Not
a
significant
mechanism
of
degradation.

3.1.2
STABILITY
IN
WATER
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

15
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
3.1.3
STABILITY
IN
SOIL
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.2
MONITORING
DATA
(
ENVIRONMENTAL)

No
data
available
3.2
TRANSPORT
AND
DISTRIBUTION
BETWEEN
ENVIRONMENTAL
COMPARTMENTS
INCLUDING
ESTIMATED
ENVIRONMENTAL
CONCENTRATIONS
AND
DISTRIBUTION
PATHWAYS
3.3.1
TRANSPORT
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.3.2
THEORETICAL
DISTRIBUTION
(
FUGACITY
CALCULATION)

Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.4
IDENTIFICATION
OF
MAIN
MODE
OF
DEGRADABILITY
IN
ACTUAL
USE
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.5
BIODEGRADATION
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.6
BOD5
,
COD
OR
RATIO
BOD5
/
COD
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
property
information.

3.7
BIOACCUMULATION
Highly
water
soluble
materials
do
not
bioaccumulate.

16
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
3.8
ADDITIONAL
REMARKS
None
4.
ECOTOXICITY
4.1
ACUTE/
PROLONGED
TOXICITY
TO
FISH
(
a)
Type
of
test:
Static
[
X
];
Semi­
static
[
];
Flow­
through
[
]
Open­
system
[
X
];
Closed­
system
[
]
Species:
Oncorhynchus
mykiss
(
Rainbow
trout)
Exposure
period:
96
hours
Results:
No
mortality
or
sublethal
effects
LC50
(
96
h)
>
103
mg/
L
NOEC
=
103
mg/
L
Analytical
monitoring:
Yes
[
X
];
No
[
];
?
[
]
Method:
OECD
guideline
203,
1992.
GLP:
Yes
[
X
]
No
[
]
?
[
]
Test
substance:
Nitrogen
solutions
as
represented
by
Urea
Ammonium
Nitrate
(
UAN)
Remarks:
No
lethal
or
sublethal
effects
were
observed
at
the
highest
test
concentration
tested.
UAN
concentrations
were
calculated
from
total
nitrate
measurements.
Measured
total
ammonia
concentrations
were
13
and
11
mg/
L
at
test
initiation
and
completion,
respectively.
Measured
un­
ionized
ammonia
concentrations
were
0.37
and
0.21
mg/
L
at
test
initiation
and
completion,
respectively.
References:
Madsen,
T.
J.
and
Bussard,
J.
B.
2000.
Acute
toxicity
of
urea
ammonium
nitrate
(
UAN)
to
the
rainbow
trout,
Oncorhynchus
mykiss,
determined
and
static
test
conditions.
ABC
Laboratories
Study
No.
46202.

(
b)
Type
of
test:
Static
[
X
];
Semi­
static
[
];
Flow­
through
[
]
Open­
system
[
];
Closed­
system
[
]
Species:
Pimephalas
promelas
(
fathead
minnow)
Exposure
period:
96
hours
Results:
LC50
between
100
and
500
mg/
L
Analytical
monitoring:
No
Method:
OECD
guideline
203,
1992
GLP:
Yes
Test
substance:
UAN
Remarks:
The
purpose
of
the
study
was
to
determine
whether
the
LD50
value
for
fathead
minnows
exceeded
1000
mg/
L,
therefore
only
a
range­
find
test
was
conducted.
No
17
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
statistical
analysis
was
performed
on
the
biological
results.
Values
are
based
on
nominal
concentrations.
References:
Madsen,
T.
J.
and
Bussard,
J.
B.
2001.
Acute
toxicity
of
urea
ammonium
nitrate
(
UAN)
to
the
fathead
minnow,
Pimephales
promelas,
determined
and
static
test
conditions.
ABC
Laboratories
Study
No.
46517.

4.2
ACUTE
TOXICITY
TO
AQUATIC
INVERTEBRATES
A.
Daphnia
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

B.
Other
aquatic
organisms
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.3
TOXICITY
TO
AQUATIC
PLANTS,
e.
g.
algae
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.4
TOXICITY
TO
BACTERIA
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.5
CHRONIC
TOXICITY
TO
AQUATIC
ORGANISMS
4.5.1
CHRONIC
TOXICITY
TO
FISH
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

18
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
4.5.2
CHRONIC
TOXICITY
TO
AQUATIC
INVERTEBRATES
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.6
TOXICITY
TO
TERRESTRIAL
ORGANISMS
4.6.1
TOXICITY
TO
SOIL
DWELLING
ORGANISMS
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.6.2
TOXICITY
TO
TERRESTRIAL
PLANTS
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.6.3
TOXICITY
TO
OTHER
NON
MAMMALIAN
TERRESTRIAL
SPECIES
(
INCLUDING
AVIAN)

Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.7
BIOLOGICAL
EFFECTS
MONITORING
(
INCLUDING
BIOMAGNIFICATION)

No
data
available
4.8
BIOTRANSFORMATION
AND
KINETICS
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

4.9
ADDITIONAL
REMARKS
None
19
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
5.
TOXICITY
5.1
ACUTE
TOXICITY
5.1.1
ACUTE
ORAL
TOXICITY
Type:
LD0
[
];
LD100
[
];
LD50
[
X
];
LDL0
[
];
Other
[
]
Species/
strain:
Sprague­
Dawley
rat
Value:
>
2,000
mg/
kg
bw
Method:
OECD
425,
"
Up
and
Down
Protocol"
An
initial
dose
of
two
thousand
milligrams
of
the
test
substance
per
kilogram
of
bodyweight
was
administered
to
one
healthy
female
rat
by
oral
gavage.
Due
to
the
absence
of
mortality
in
this
animal,
a
second
female
received
the
same
dose
level.
Following
the
completion
of
dosing
and
100%
survival
in
a
total
of
three
females,
a
group
of
three
males
was
tested
(
simultaneously)
at
the
above
dose
level.
All
animals
were
observed
for
mortality,
signs
of
gross
toxicity,
and
behavioral
changes
at
least
once
daily
for
14
days
after
dosing.
Bodyweights
were
recorded
prior
to
administration
and
again
on
days
7
and
14
(
termination)
after
dosing.
Necropsies
were
performed
on
all
animals
at
terminal
sacrifice.
GLP:
Yes
[
X
]
No
[
]
?
[
]
Test
substance:
Nitrogen
solutions
as
represented
by
Urea
Ammonium
Nitrate
(
UAN)
(
clear
colorless
liquid)
Remarks:
All
animals
survived,
gained
weight
and
appeared
active
and
healthy
throughout
the
test.
No
signs
of
toxicity
were
observed.
References:
Merkel,
D.
J.
2000.
Acute
oral
toxicity:
Up
and
down
procedure
in
rats­
limit
test.
Product
Safety
Labs
study
number
9739.

5.1.2
ACUTE
INHALATION
TOXICITY
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.1.3
ACUTE
DERMAL
TOXICITY
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

20
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
5.1.4
ACUTE
TOXICITY
BY
OTHER
ROUTES
OF
ADMINISTRATION
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.2
CORROSIVENESS/
IRRITATION
5.2.1
SKIN
IRRITATION/
CORROSION
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.2.2
EYE
IRRITATION/
CORROSION
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.3
SKIN
SENSITIZATION
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.4
REPEATED
DOSE
TOXICITY
Results:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.5
GENETIC
TOXICITY
IN
VITRO
A.
Bacterial
Test
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

21
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
B.
Non­
bacterial
in
vitro
test
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.6
GENETIC
TOXICITY
IN
VIVO
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.7
CARCINOGENICITY
Remarks:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.8
TOXICITY
TO
REPRODUCTION
Results:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.9
DEVELOPMENTAL
TOXICITY/
TERATOGENICITY
Results:
Nitrogen
solutions
(
UAN)
is
a
blend
of
urea,
ammonium
nitrate
and
water.
Refer
to
the
individual
materials
for
toxicity
information.

5.10
OTHER
RELEVANT
INFORMATION
No
further
information
available
5.11
EXPERIENCE
WITH
HUMAN
EXPOSURE
No
data
available
22
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
6.
REFERENCES
Merkel,
D.
J.
2000.
Acute
oral
toxicity:
Up
and
down
procedure
in
rats­
limit
test.
Product
Safety
Labs
Study
Number
9739.

Madsen,
T.
J.
and
Bussard,
J.
B.
2000.
Acute
toxicity
of
urea
ammonium
nitrate
(
UAN)
to
the
rainbow
trout,
Oncorhynchus
mykiss,
determined
and
static
test
conditions.
ABC
Laboratories
Study
No.
46202.

Madsen,
T.
J.
and
Bussard,
J.
B.
2001.
Acute
toxicity
of
urea
ammonium
nitrate
(
UAN)
to
the
fathead
minnow,
Pimephales
promelas,
determined
and
static
test
conditions.
ABC
Laboratories
Study
No.
46517.

United
Nations
Environment
Programme
(
UNEP).
1998.
Mineral
Fertilizer
Production
and
the
Environment:
Part
1.
The
Fertilizer
Industry's
Manufacturing
Processes
and
Environmental
Issues.
In
collaboration
with
the
International
Fertilizer
Industry
Association.
Technical
Report
No.
26.

United
Nations
Industrial
Development
Organization
(
UNIDO)
and
International
Fertilizer
Development
Center
(
IFDC).
1998.
Fertilizer
Manual.
Kluwer
Academic
Publisher,
The
Netherlands.

23
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
APPENDIX
A
 
SIDS
Data
Availability
Summary
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
Explanation
of
Appendix
A
Appendix
A
is
a
SIDS
Data
Summary
table
that
provides
an
overview
of
availability
and
quality
of
the
data
used
in
this
Health
and
Environmental
Safety
Data
Summary
for
Nitrogen
Solutions
(
UAN).
Y
(
Yes)
and
N
(
No)
designations
indicate
whether
data
are
available
that
meet
the
criteria
for
each
column.
The
columns
are
described
as:

Information:
Data
exist
that
were
useful
for
describing
the
data
element.
These
data
may
be
from
standard
laboratory
tests
or
from
generally
recognized
published
sources
or
professional
experience.

OECD
Study:
The
data
were
developed
using
standard
OECD
or
essentially
similar
(
e.
g.,
EPA
harmonized
protocols)
guidelines.

GLP:
The
data
were
developed
under
standard
Good
Laboratory
Practice
provisions.
These
generally
represent
the
highest
quality
data.

Other
Study:
Studies
were
conducted
that
could
not
be
definitively
identified
as
following
OECD
or
GLP
protocols.
These
studies
were
included
when
they
were
determined
to
be
of
adequate
quality
and
provided
relevant
information
to
the
characterization
of
the
compound.

Estimation
Method:
In
some
cases,
data
may
be
estimated
using
established
structure
activity
relationships.
These
methods
are
common
for
physical­
chemical
parameters
such
as
octanol­
water
partition
coefficients
and
water
solubility.

Acceptable:
This
column
indicates
whether
the
data
are
deemed
acceptable
by
standard
acceptability
criteria
and
professional
judgment.
Only
the
data
meeting
adequacy
standards
are
included
in
this
summary
document.

SIDS
Testing
Recommended:
This
column
indicates
whether
additional
testing
is
recommended
based
on
an
evaluation
of
the
available
data
summarized
in
this
document.
It
is
generally
not
necessary
to
fill
all
of
the
apparent
data
gaps
in
order
to
adequately
characterize
the
inherent
hazard
of
chemicals.
Information
from
other
data
elements
and
from
other
chemicals
in
the
category,
along
with
professional
judgment,
are
useful
in
the
final
determination
of
the
need
for
further
testing.

Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
SIDS
DATA
AVAILABILITY
SUMMARY
DATE:
January
27,
2003
Nitrogen
Solutions
(
UAN)
CAS
No:
15978­
77­
5
Information
OECD
Study
GLP
Other
Study
Estimated
Method
Acceptable
SIDS
Testing
Recommended
STUDY
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
N
N
N
N
N
­
N
2.2
Boiling
Point
N
N
N
N
N
­
N
2.3
Density
N
N
N
N
N
­
N
2.4
Vapor
Pressure
N
N
N
N
N
­
N
2.5
Partition
Coefficient
N
N
N
N
N
­
N
2.6
Water
Solubility
Y
N
N
N
N
Y
N
pH
and
pKa
Values
Y
N
N
Y
N
Y
N
2.8
Auto
Flammability
N
N
N
N
N
­
N
2.9
Flammability
N
N
N
N
N
­
N
2.10
Explosive
Properties
N
N
N
N
N
­
N
2.11
Oxidizing
Properties
N
N
N
N
N
­
N
2.12
Oxidation:
Reduction
Potential
N
N
N
N
N
­
N
OTHER
P/
C
STUDIES
RECEIVED
N
N
N
N
N
­
N
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
Y
N
N
N
N
Y
N
3.1.2
Stability
in
Water
N
N
N
N
N
­
N
3.1.3
Stability
in
Soil
N
N
N
N
N
­
N
3.2
Monitoring
Data
N
N
N
N
N
­
N
3.3
Transport
and
Distribution
N
N
N
N
N
­
N
3.5
Biodegradation
N
N
N
N
N
­
N
3.7
Bioaccumulation
N
N
N
N
N
­
N
OTHER
ENV
FATE
STUDIES
RECEIVED
N
N
N
N
N
­
N
ECOTOXICITY
4.1
Acute
Toxicity
to
Fish
Y
Y
Y
Y
N
Y
N
4.2
Acute
Toxicity
to
Daphnia
N
N
N
N
N
­
N
4.3
Toxicity
to
Algae
N
N
N
N
N
­
N
4.5.1
Chronic
Toxicity
to
Fish
N
N
N
N
N
­
N
4.5.2
Chronic
Toxicity
to
Daphnia
N
N
N
N
N
­
N
4.6.1
Toxicity
to
Soil
Dwelling
Organisms
N
N
N
N
N
­
N
4.6.2
Toxicity
to
Terrestrial
Plants
N
N
N
N
N
­
N
4.6.3
Toxicity
to
Birds
N
N
N
N
N
­
N
OTHER
ECOTOXICITY
STUDIES
RECEIVED
N
N
N
N
N
­
N
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
Nitrogen
Solutions
(
UAN)
CAS
No:
15978­
77­
5
Information
OECD
Study
GLP
Other
Study
Estimated
Method
Acceptable
SIDS
Testing
Recommended
STUDY
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
Y/
N
TOXICITY
5.1.1
Acute
Oral
Y
Y
Y
N
N
Y
N
5.1.2
Acute
Inhalation
N
N
N
N
N
­
N
5.1.3
Acute
Dermal
N
N
N
N
N
­
N
5.1.4
Acute
Toxicity
by
Other
Routes
of
Administration
N
N
N
N
N
­
N
5.2.1
Skin
Irritation
N
N
N
N
N
­
N
5.2.2
Eye
Irritation
N
N
N
N
N
­
N
5.3
Skin
Sensitization
N
N
N
N
N
­
N
5.4
Repeated
Dose
N
N
N
N
N
­
N
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
N
N
N
N
N
­
N
.
Chromosomal
aberration
N
N
N
N
N
­
N
5.6
Genetic
Toxicity
in
vivo
N
N
N
N
N
­
N
5.7
Carcinogenicity
N
N
N
N
N
­
N
5.8
Reproduction
Toxicity
N
N
N
N
N
­
N
5.9
Development/
Teratogenicity
N
N
N
N
N
­
N
5.11
Human
Experience
N
N
N
N
N
­
N
OTHER
TOXICITY
STUDIES
RECEIVED
N
N
N
N
N
­
N
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
APPENDIX
B
 
SIDS
Data
Summaries
for
the
Nitrate
Compounds
Category:
Ammonium
Nitrate,
Nitrogen
Solutions
(
UAN),
Potassium
Nitrate,
Potassium
Sodium
Nitrate,
and
Sodium
Nitrate
Nitrogen
Solutions
(
UAN)
CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Ammonium
Nitrate
Nitrogen
Solution
(
UAN)

SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
169.6o
C
2.2
Boiling
Point
210o
C
2.3
Density
1.725
g/
mL
at
25o
C
2.4
Vapor
Pressure
15
hPa
at
20o
C
2.5
Octanol/
Water
Partition
Coefficient
OECD
107
­
3.1
at
25o
C
2.6A
Water
Solubility
1,920
g/
L
at
20o
C
2.6B
pH
and
pKa
values
pH
=
5.43
in
aqueous
solution
2.7
FlashPoint
Not
applicable
2.8
Auto
Flammability
Not
flammable
2.9
Flammability
Not
flammable
2.10
Explosive
Properties
May
explodeunder
high
temperature
or
strong
shocks
2.11
Oxidizing
Properties
Strong
oxidizer
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Ammonium
Nitrate
Nitrogen
Solution
(
UAN)

SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
Does
not
photodegrade
3.12
Stability
inWater
Stable
3.1.3
Stability
in
Soil
Binds
to
clay
particles
3.3
Transport
and
Distribution
3.3.2
Distribution
Calculated,

Fugacity
Level
III
0.251%
to
air
45.4%
to
water
54.2%
to
soil
0.0757%
to
sediment
3.5
Biodegradation
Readily
biodegraded
3.7
Bioaccumulation
Does
not
bioaccumulate
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Ammonium
Nitrate
Nitrogen
Solution
(
UAN)

SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ECOTOXICITY
4.1
Acute
toxicity
to
Fish
Cyprinus
carpio
L.
48
hrs
LC50
=
1.15
 
1.72
mg
unionized
NH3
/
L
Oncorhyn­

chus
mykiss
96
hrs
LC50
>
103
mg/
L
Chinook
salmon,
rainbow
trout,
bluegill
96
hrs
LC50
=
420
 
1,360
mg
NO3
/
L
Pimephalas
promelas
96
hrs
LC50
between
100
and
500
mg/
L
4.2
Acute
Toxicityto
Aquatic
Invertebrates
Daphnia
magna
EC50
=
555
mg/
L
4.3
Toxicity
to
Aquatic
Plants
(
Algae)
Scenedesmus
quadricauda
EC50
=
83
mg/
L
4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
Bullia
digitalis
Up
to
7
days
NOEC
=
300
mg/
L
4.6.1
Toxicity
to
Soil
Dwelling
Organisms
4.6.2
Toxicity
to
Terrestrial
Plants
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Ammonium
Nitrate
Nitrogen
Solution
(
UAN)

SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
TOXICITY
5.1.1
Acute
Oral
Toxicity
Rat
OECD401
LD50
=
2,800
mg/
kg
bw
Rat
OECD425
LD50
>
2,000
mg/
kg
bw
Rat
OECD
401
LD50
=
2,462
mg/
kg
bw
Rat
OECD
401
LD50
=
2,950
mg/
kg
bw
Rat
LD50
=
4,500
mg/
kg
bw
5.1.2
Acute
Inhalation
Rat
4
hrs
LC50
>
88.8
mg/
L
5.1.3
Acute
Dermal
Toxicity
Rat
OECD402
LC50
>
5,000
mg/
kg
5.1.4
Acute
Toxicity,

Other
Routes
White
rat
Injected;
3
hrs
Minimum
lethal
dose
=
0.065
mg
NH4
NO3
 
N
5.2.1
Skin
Irritation/

Corrosion
Rabbit
Moderately
irritating
Rabbit
Not
irritating
5.2.2
Eye
Irritation/
corrosion
5.3
Skin
Sensitization
5.4
Repeated
Dose
Rat
Inhalation;
2
weeks
NOAEL
=
185
mg/
m3
Rat
Inhalation;
4
weeks
NOAEL
=
1
mg/
m3
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
Salmonella
typhimurium
Bacterial
reverse
mutation
assay
Negative
.
Chromosomal
aberration
5.6
Genetic
Toxicity
in
vivo
5.7
Carcinogenicity
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
Ammonium
Nitrate
Nitrogen
Solution
(
UAN)

DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
5.8
Toxicity
to
Reproduction
5.9
Development
Toxicity/
Teratogenicity
Rat
NOAEL
>
57
mg/
kg/
day
51.0
Additional
Information
No
sensory
irritation
5.11
Human
Experience
See
text
1.8
Occupational
Exposure
Limits
TLV
as
inert
dust
15
mg/
m3
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
PHYSICAL­
CHEMICAL
DATA
2.1
Melting
Point
344o
C
308o
C;
307o
C
2.2
Boiling
Point
400o
C
at
760
mm
Hg
380o
C
at
760
mm
Hg
2.3
Density
2.109
g/
mL
at
16o
C
2.26
g/
cm3
at
20o
C
2.4
Vapor
Pressure
EPISUITE
V.
3.10
1.39x10­
15
hPa
at
25o
C
Not
expected
to
volatilize
2.5
Octanol/
Water
Partition
Coefficient
EPISUITE
V.
3.10
­
0.79
at
25o
C
OECD
107
­
3.8
at
25o
C
2.6A
Water
Solubility
357
g/
L
at
25o
C
874
g/
L
at
20o
C
2.6B
pH
and
pKa
values
pH
~
7
at
25o
C
pH
=
7
in
aqueous
solution
2.7
FlashPoint
Not
applicable
Not
applicable
2.8
Auto
Flammability
Not
flammable
Does
not
burn
2.9
Flammability
Not
flammable
Not
flammable
2.10
Explosive
Properties
May
explodeon
contact
with
reducing
agents
and/
or
impact.
May
explodeat
high
temperatures
or
contact
with
reducing
agents
2.11
Oxidizing
Properties
Strong
oxidizer
Strong
oxidizer
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ENVIRONMENTAL
FATE
and
PATHWAY
3.1.1
Photodegradation
Does
not
photodegrade
Doesnot
photodegrade
Doesnot
photodegrade
3.1.2
Stability
inWater
Stable
Stable
3.1.3
Stability
in
Soil
Stable
Binds
to
clay
particles
3.3.2
Distribution
Fugacity
Level
III
(
Mackay
type)
8.34x10­
9
%
to
air
45.3%
to
water
54.7%
to
soil
0.0755%
to
sediment
FugacityLevel
III
(
Mackay
type)
0.001%
to
air
45.3%
to
water
54.7%
to
soil
0.076%
to
sediment
3.5
Biodegradation
Biodegradable
Biodegradable
3.7
Bioaccumulation
Does
not
bioaccumulate
Doesnot
bioaccumulate
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
ECOTOXICITY
4.1
Acute
toxicity
to
Fish
Lepomis
macrochirus
96
hrs
LC50
=
1,839
mg
NO3
/
L
Oncorhyn­

chus
mykiss
96
hrs
LC50
>
98.9
mg/
L
Lepomis
macrochirus
96
hrs
LC50
>
8,753
mg
NO3
/
L
Lepomis
macrochirus
24
hrs
TLm
=
9,331
mg
NO3
/
L
Oncorhyn­

chus
tshawtscha
96
hrs
+
TLm
(
freshwater,

salmon)
=
5,800
mg
NO3
/
L
TLm
(
15
ppt
salinity,
salmon)
=

4,400
mg
NO3/
L
Salmo
gairdneri
(
rainbow
trout)
TLm
(
freshwater,

trout)
=
6,000
mg
NO3
/
L
TLm
(
15
ppt
salinity,
trout)
=

4,650
mg
NO3/
L
4.2
Acute
Toxicityto
Aquatic
Invertebrates
Daphnia
magna
48
hrs
TLm
=
300
mg
NO3
/
L
Daphnia
magna
48
hrs
TLm
=
3,581
mg
NaNO3
/
L
Various
penaeid
shrimp
48
hrs
LC50
=
3,400
mg
NO3
­
N/
L
NOEC
=
2,000
mg
NO3
­
N/
L
Penaeus
monodon
larvae
40
hrs
LC50
>
100
mg
NO3
/
L
Crassostrea
virginica
96
hrs
LC50
=
15,810
mg
NO3
/
L
(
adults);

23,040
mg
NO3
/
L
(
juveniles)

Mercenaria
mercenaria
96
hrs
LC50
>
19,840
mg
NO3
/
L
(
adults
and
juveniles)

4.3
Toxicity
to
Aquatic
Plants
(
Algae)
Seetext
Scenedes­

mus
quadricauda
Toxicity
threshold
=
1,233
mg
NO2
­/
L
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
4.5.1
Chronic
Toxicity
to
Fish
Amphiprion
oscellaris
3
months
%
survival
in
larvae
(
low­
nitrate
system)
=
37%

%
survival
in
larvae
(
high­
nitrate
system)
=
34%

4.5.2
Chronic
Toxicity
to
Aquatic
Invertebrates
Nereis
grubei
Neanthes
arenaceo­

dentata
Dorvilliea
articulata
Capitella
capita
TLm
=
327
mg
NO3
/
L
TLm
=
496
mg
NO3
/
L
TLm
=
880
mg
NO3
/
L
TLm
=
713
mg
NO3/
L
Penaeus
monodon
3
 
5
weeks
LC50
>
200
mg
NO3
 
N/
L
4.6.3
Toxicity
to
Other
Non­
Mammalian
Terrestrial
Species
Coturnix
coturnix
japonica
(
Japanese
quail)
7
days
to
15
weeks
LC100
=
5,280
ppm
(
by
day
3
post
treatment)

LC100
=
5,940
ppm
(
by
day
4
post
treatment)

Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
DATA
ELEMENTS
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
TOXICITY
5.1.1
Acute
Oral
Toxicity
Rat
LD50
=
3,750
mg/
kg
bw
Rat
OECD425
LD50
>
2,000
mg/
kg
bw
Rabbit
LD50
=
2,680
mg/
kg
bw
Rabbit
LD50
=
1,166
NO3
/
kg
Rat
LD50
=
1,267,

3,236,
3,430,
and
5,200
mg/
kg
bw
Cow,
Sheep
Lethal
dose
(
cow)

=
75­
140
mg
NO3
­

N/
kg
Lethal
dose
(
sheep)
=
70
mg
NO3
­
N/
kg
Goat
LD100
=
1,000­

1,500
mg/
kg
5.1.2
Acute
Inhalation
Dog,
sheep
7.5
minutes
to
4
hrs
NOEC
(
dog)
=
5
mg/
m3
NOEC
(
sheep)
=
1
mg/
m3
5.1.3
Acute
Dermal
Toxicity
Rat
OECD402
LD50
>
5,000
mg/
kg
5.1.4
Acute
Toxicity,

Other
Routes
Dogs
i.
v.;
60
minutes
NOEC
=
100
mg
NaNO3
5.4
Repeated
Dose
Rat
OECD
422
NOAEL
=
1,500
mg/
kg/
day
Rat
6
weeks
MTD
=
5%
in
the
diet
Rat
14
months
See
text
5.5
Genetic
Toxicity
in
vitro
.
Gene
mutation
Salmonella
typhimurium
Bacterial
reverse
mutation
assay
Negative
Salmonella
typhimurium
Bacterial
reverse
mutations
assay
Negative
Bacillus
subtilis
recombination
assay
Negative
.
Chromosomal
aberration
Chinese
hamster
ovary
cells
Chromosome
aberration
test
Positive
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
SIDS
DATA
SUMMARIES
FOR
NITRATES
CATEGORY
Date:
January
27,
2003
Nitrogen
Solutions
(
UAN)

CAS
#
15978­
77­
5
Potassium
Nitrate
Potassium
Sodium
Nitrate
Sodium
Nitrate
DATA
ELEMENTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
SPECIES
PROTOCOL
RESULTS
5.6
Gs
enetic
Toxicity
in
vivo
Ra
t
10
week
Negative
Swiss
mice
and
Wistar
rats
Micronucleus
assay
Positive
ce
e
Mi
Mousgerm
cells
Positive
5.7
Carcinogenicity
Rats
104
weeks
Not
significantly
different
from
controls
5.8
T
oxicity
to
Reproduction
Rat
OECD
422
NOAEL
=
1,500
mg/
kg/
day
Beagledog
Two­
genera
study
tion
No
significant
adverse
effects
Guineapig
tion
One­
genera
study
NOAEL
Parental
=

10,000
ppm
NOAELF1
Offspring
=
10,000
ppm
Rat
tion
Two­
genera
study
NOAEL
Parental
=

2.5%

5.9
Dt
e,
t
evelopmen
Toxicity/
Teratogenicity
Rat
OECD
422
NOAEL
=
1,500
mg/
kg/
day
Rat,
Mous
Rabbit,

Syrian
hamster
No
significan
adverse
effects
Mouse
Oral;
10
day
exposure
NOAEL
>
400
mg/
kg
bw
5.10
Mend
Read
tabolism
a
Toxicokinetics
ily
absorbed
and
excreted
5.11
Human
Experience
See
text
See
text
1.8
Ol
ccupationa
Exposure
Limits
TLV
as
inert
dust
15
mg/
m3
TLV
as
inert
dust
15
mg/
m3
TLV
as
inert
dust
15
mg/
m3
