FINAL
REPORT
STUDY
TITLE
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,1,2­
Trichloroethane
(1,
l
,ZTCE)
in
Rats
STUDY
DIRECTOR
Daniel
T.
Kirkpatrick,
Ph.
D.,
D.
A.
B.
T.

STUDY
INITIATED
ON
December
21,200O
STUDY
COMPLETED
ON
October
29,200l
PERFORMING
LABORATORY
WIL
Research
Laboratories,
Inc.
1407
George
Road
Ashland,
OH
44805928
1
LABORATORY
STUDY
NUMBER
WlL­
417001
SPONSOR
Task
Force
c/
o
Regulatory
Sciences
International
P.
O.
Box
33
1
Millwood,
VA
22314
Page
1
of
311
WILRE~
EARCHLABORATORJES,
INC.
1407GEoRGE~
0m
ASHLAND.°
H
448059281
(419)
289­
8700
~AX(
419)
289­
3650
improving
human
health
and
protecting
the
environment
rhrotlglrscimtific
research
services.@
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1
.l
.ZTrichloroethane
(1
.I
.ZTCE)
in
Rats
COMPLIANCE
STATEMENT
This
study,
designated
WIL­
417001,
was
conducted
in
compliance
with
the
Environmental
Protection
Agency
Toxic
Substances
Control
Act
(EPA­
TSCA)
Good
Laboratory
Practice
Regulations
(40
CFR
Part
792),
the
standard
operating
procedures
of
WIL
Research
Laboratories,
Inc.,
and
the
protocol
as
approved
by
the
sponsor.
A
Certificate
of
Analysis
(Appendix
A)
was
supplied
by
the
sponsor;
it
is
unknown
whether
the
characterization
analysis
was
conducted
according
to
Good
Laboratory
Practices.

Study
Director:

­2­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1.1.2­
Trichloroethane
(l.
l.
ZTCE)
in
Rats
TABLE
OF
CONTENTS
SLllnlllaly
Introduction
Experimental
Design
Materials
and
Methods
Observations
Statistical
Methods
Results
Conclusion
Personnel
and
Report
Submission
Quality
Assurance
Unit
Statement
References
Deviations
from
the
Protocol
INDEX
OF
FIGURES
1:
Atmosphere
Generation
and
Exposure
System
(Low
and
Mid
Concentration
Exposure
Groups)

2:
Atmosphere
Generation
and
Exposure
System
(High
Concentration
Exposure
Group)
&g
6
10
11
13
20
24
25
32
35
36
37
38
l&
g
39
40
­3­
WrL­
417001
Hazardous
Air
Pollutant
Task
Force
1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

11.

12.

13.

14.

15.

16.

17.

18.

19.

20.
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1
.1.2­
Trichloroethane
(1.1
.Z­
TCE)
in
Rats
INDEX
OF
TABLES
Individual
Test
Atmosphere
Concentrations
Summary
of
Clinical
Findings
­
Total
Occurrence/
No.
of
Animals
(Detailed
Physical
Examinations/
Dispositions)

Summary
of
Clinical
Findings
­
Total
Occurrence/
No.
of
Animals
(Immediately
Following
Exposure)

Response
to
Novel
Stimulus
­
Summary
of
Incidence
Body
Weights
(Grams)
­
Summary
of
Means
Body
Weight
Gains
(Grams)
­
Summary
of
Means
Bronchoalveolar
Lavage
Fluid
Values
­
Summary
of
Means
Gross
Necropsy
Observations
Incidence
Summary
(Unscheduled
Deaths)

Gross
Necropsy
Observations
Incidence
Summary
(Scheduled
Necropsy)

Organ
Weights
(Grams)
­
Summary
of
Means
Organ
Weights
Relative
to
Final
Body
Weights
(Grams/
100
Grams)
­
Summary
of
Means
Histomorphological
Diagnosis
­
Summary
Incidence
(Unscheduled
Deaths)

Histomorphological
Diagnosis
­
Summary
Incidence
(Scheduled
Necropsy)

Individual
Clinical
Observations
(Detailed
Physical
Examinations/
Dispositions)

Individual
Clinical
Observations
(Immediately
Following
Exposure)

Individual
Response
to
Novel
Stimulus
Individual
Body
Weights
(Grams)

Individual
Body
Weight
Gains
(Grams)

Individual
Bronchoalveolar
Lavage
Fluid
Values
Individual
Gross
and
Microscopic
Description
of
Organs
(Unscheduled
Deaths)
Pa.
g;
e
42
45
52
56
60
64
68
70
71
72
76
80
88
101
110
116
120
136
152
160
­4­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
21.

22.

23.

A.

B.

c.

D.

E.

F.

G.
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,
1
.2­
Trichloroethane
(
1,1,2­
TCE)
in
Rats
JNDEX
OF
TABLES
tndividual
Gross
and
Microscopic
Description
of
Organs
(Scheduled
Necropsy)

Individual
Organ
Weights
and
Final
Body
Weights
(Grams)

tndividual
Organ
Weights
Relative
to
Final
Body
Weights
(Grams/
100
Grams)

INDEX
OF
APPENDICES
Certificate
of
AnaIysis
(Sponsor­
Provided
Data)

Serological
Screening
(BioReliance
Corporation)

Pretest
Clinical
Observations
Clinical
Pathology
Methods,
Procedures
and
References
Bronchoalveolar
Lavage
Cytology
Report
(Gail
L.
Walter,
D.
V.
M.,
D.
A.
C.
V.
P.,
D.
A.
B.
T.,
Clinical
Pathology
Consultant)

Results
of
Phagocytosis
and
Respiratory
Burst
Assays
(Gary
R.
Burleson,
Ph.
D.,
Burleson
Research
Technologies)

Study
Protocol
166
220
22s
ii2422
236
238
242
245
247
264
292
­5­
WL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
SUMMARY
The
toxicological
response,
including
exposure
level­
response
relationships,
of
the
test
article
in
rats
was
evaluated
following
a
single,
four­
hour
exposure.
The
test
article
was
administered
to
four
groups
of
10
male
and
10
female
rats
as
a
vapor
via
whole­
body
exposure
at
target
concentrations
of
0,
50,
200
and
1500
ppm.
Five
animals/
sex/
group
were
assigned
for
anatomical
pathology
evaluation
(gross
and
microscopic
examinations)
and
five
animals/
sex/
group
were
assigned
for
evaluation
of
bronchoalveolar
lavage
fluid
(BALF).
For
the
female
BALF
evaluation,
the
target
concentration
for
the
high
exposure
level
was
decreased
to
1000
ppm
based
on
the
death
of
three
females
following
exposure
targeted
at
1500
ppm.
Following
exposure,
the
animals
were
maintained
for
approximately
24­
hours
prior
to
euthanasia.
Body
weights
were
determined
and
observations
for
clinical
signs
of
toxicity
were
conducted
at
the
appropriate
intervals
for
all
animals.

Three
females
in
the
1527
ppm
group
and
three
females
in
the
840
ppm
group
were
found
dead
or
euthanized
in
extremis
as
a
result
of
test
article
exposure.

Animals
in
the
high
concentration
groups
(1527,
1474
and
840
ppm)
showed
no
reaction
to
novel
stimulus
(a
sudden
noise)
at
the
approximate
midpoint
of
exposure.
All
other
test
article
groups
reacted
similarly
to
the
control
group
and
were
considered
normal.
Immediately
following
exposure,
all
animals
in
the
high
concentration
groups
(1527
ppm
and
1474
or
840
ppm)
appeared
to
be
sleeping,
were
lethargic,
ataxic,
had
decreased
respiration
rates
and/
or
clear
discharge
of
the
eyes.
These
clinical
effects
observed
for
high
concentration
animals
during
or
immediately
following
exposure
were
primarily
associated
with
anesthetic
or
narcotic
effects
of
the
test
article,
a
chlorinated,
short­
chain
hydrocarbon.
On
the
day
following
exposure,
ataxia,
decreased
respiration
rate,
lethargy,
hypothermia,
reddish
brown
urine
and/
or
clear
discharge
of
the
eye(
s)
were
observed
in
the
high
concentration
groups.
There
were
no
other
toxicologically
significant
clinical
observations
immediately
following
or
on
the
day
following
exposure.

Test
article­
related
body
weight
effects
were
also
limited
to
the
high
concentration
groups.
Decreased
mean
body
weight
gains
were
observed
in
the
1527
ppm
group
males
and
females
and
in
the
1474
ppm
group
males
and
840
ppm
group
females
on
the
day
following
exposure
(day
1)
when
compared
to
the
control
group.
As
a
result,
mean
body
weights
were
decreased
in
the
1527
ppm
group
males
and
females
and
1474
ppm
group
males
on
day
1.
There
were
no
other
remarkable
changes
in
body
weight
data.

­6­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Mean
total
protein
was
increased
in
the
bronchoalveolar
lavage
fluid
of
the
1474
ppm
group
males
and
840
ppm
group
females.
No
microscopic
findings
in
the
lung
correlated
with
this
increase
in
lavage
fluid
total
protein.
Additionally,
no
alterations
in
BALF
total
or
differential
cell
counts
attributed
to
test
article
exposure
were
observed
at
any
concentration.
The
functional
capacity
of
alveolar
macrophages
isolated
from
bronchoalveolar
lavage
fluid
was
also
evaluated.
Phagocytic
activity
was
reduced
in
all
male
1,1,2­
TCE
exposure
groups
with
the
greatest
effect
in
the
mid­
concentration
group.
For
females,
phagocytic
activity
appeared
to
be
decreased
only
in
the
mid­
concentration
group.
It
is
noteworthy
that
there
was
no
exposure
concentration­
dependence
for
the
apparent
effects
on
phagocytic
activity
in
males.
In
addition,
historical
control
data,
which
would
provide
normal
or
typical
values
for
phagocytic
activity
of
alveolar
macrophages
from
Fischer
344
rats
(determined
using
the
same
assay
method),
is
not
available.
The
toxicological
significance
of
the
changes
observed
in
this
study
is
uncertain
based
on
the
lack
of
exposure
concentration­
dependence
and
the
unavailability
of
historical
control
data.
There
were
no
test
article­
related
effects
on
respiratory
burst
activity
at
any
concentration.

For
the
female
rats
from
the
1527
ppm
group
that
died
prior
to
the
scheduled
necropsy,
the
only
internal
macroscopic
finding
at
necropsy
was
a
dark
red
area
in
the
glandular
portion
of
the
stomach
of
one
animal.
At
the
scheduled
necropsy,
dark
red
area(
s)
on
the
liver
were
observed
for
two
males
and
a
pale
liver
was
observed
for
one
female
in
the
1527
ppm
group,
Dark
red
areas
in
the
glandular
and/
or
non­
glandular
portions
of
the
stomach
were
observed
for
one
male
in
the
1527
ppm
group
and
dark
red
contents
in
the
cecum,
ileum,
jejunum
and/
or
stomach
were
observed
for
four
males
and
one
female
in
the
1527
ppm
group.
Dark
red
contents
in
the
urinary
bladder
were
also
observed
for
one
male
in
the
1527
ppm
group.
There
were
no
other
internal
macroscopic
findings
at
the
scheduled
necropsy.
Mean
relative
(to
final
body
weight)
kidney
weight
in
the
1527
ppm
group
males
was
significantly
(~~
0.01)
higher
than
the
control
group.
The
increase
correlated
with
the
microscopic
findings
noted
for
the
kidney.
There
were
no
other
test
article­
related
effects
on
organ
weights.

Test
article­
related
microscopic
changes
were
seen
in
the
liver,
nasal
cavity,
larynx,
kidney
and
stomach.
In
the
liver,
centrilobular
hepatocellular
necrosis
(moderate
or
severe)
was
observed
in
all
animals
in
the
1527
ppm
group
and
in
4/
5
females
in
the
18
1
ppm
group.
Chlorinated,
short­
chain
hydrocarbons,
including
1
,I
,2­
trichloroethane,
have
been
widely
reported
to
produce
cell
death
in
the
centrilobular
areas
of
the
liver.
The
localization
of
liver
injury
appears
to
be
related
to
the
distribution
of
microsomal
metabolizing
enzymes.
A
relatively
high
concentration
of
microsomal
metabolizing
enzymes
is
also
present
in
the
rodent
nasal
olfactory
epithelium.
In
the
current
study,
minimal
to
severe
necrosis
was
observed
in
multiple
nasal
sections
of
all
181
and
1527
ppm
group
males
and
females
with
severity
tending
to
increase
with
exposure
level
and
proximity
to
the
nares.
At
58
ppm,
all
females
and
2/
5
males
had
minimal
to
mild
­7­
w&
417001
Hazardous
Air
Pollutant
Task
Force
olfactory
epithelial
necrosis
in
at
least
one
nasal
section.
The
nasal
olfactory
epithelium
of
the
rat
is
extremely
sensitive
to
agents
that
may
be
activated
by
cytochrome
P­
450
metabolizing
enzymes.
It
has
been
reported
that
the
fraction
of
the
nasal
airways
covered
by
olfactory
epithelium
is
approximately
50%
in
the
F344
rat
compared
to
3%
in
the
human6.
In
addition,
based
on
information
cited
by
Haschek
and
Witschi,
the
relative
surface
area
of
the
nasal
airways
(normalized
to
body
weight)
is
approximately
16
times
greater
in
the
rat
compared
to
the
human'.
As
a
result,
the
rat
may
be
considered
one
of
the
most
sensitive
animal
models
for
this
type
of
airway
epithelial
lesion.
The
finding
of
minimal
or
mild
olfactory
epithelial
cell
necrosis
for
animals
from
the
58
ppm
group
is
clearly
test
article­
related,
but
it
is
considered
unlikely
to
represent
a
toxicologically
adverse
response.
It
is
likely
that
such
minor
effects
involving
a
small
fraction
of
the
olfactory
epithelium
would
completely
resolve
following
a
single
exposure.

Single
cell
necrosis
of
the
transitional
epithelium
was
seen
in
the
turbinates
and
lateral
wall
of
nasal
sections
II
and,
variably,
IlI
from
all
males
and
females
in
the
1527
ppm
group.
Acute
inflammation
of
the
larynx
was
observed
in
all
females
and
3/
5
males
in
the
1527
ppm
group.
Four
of
five
males
in
the
58
ppm
group
and
2/
5
males
in
the
181
ppm
group
also
had
minimal
laryngeal
inflammation.
It
was
unclear
why
the
highest
incidence
of
this
change
was
observed
in
the
lowest
concentration
group.
The
ventral
pouch
of
the
larynx
was
the
most
commonly
affected
area.
The
transitional
epithelium
of
the
larynx
in
the
rat
has
also
been
found
to
be
extremely
sensitive
to
injury
by
a
broad
range
of
chemical
agents6.
The
finding
of
minimal
laryngeal
inflammation
with
no
concentration­
dependence
suggests
that
this
finding
has
little
or
no
toxicological
significance.

A
small
number
of
renal
cortical
tubules
in
the
1527
ppm
group
contained
a
brightly
eosinophilic
to
basophilic
coarsely
granular
material.
The
location
of
this
luminal
debris
was
nonspecific
and
the
composition
and
significance
of
this
change
was
unclear.
In
the
glandular
stomach,
ulceration
and/
or
erosion
was
observed
at
the
1527
ppm
exposure
level.
This
change
was
not
seen
in
any
other
groups
and
was
the
most
likely
source
of
the
dark
red
areas
and
contents
seen
in
the
stomach
at
necropsy.
This
change
may
be
secondary
to
stress
and
not
a
direct
effect
of
1,1,2­
TCE
exposure.
No
other
test
article­
related
microscopic
changes
were
observed.

Based
on
the
microscopic
findings
(olfactory
epithelial
necrosis
and
acute
laryngeal
inflammation)
and
effects
on
phagocytic
activity
of
alveolar
macrophages
from
bronchoalveolar
lavage
fluid,
the
no­
observed­
effect
level
(NOEL)
for
males
and
females
was
less
than
58
ppm
when
exposed
to
1,1,2­
trichloroethane
as
a
single,
four­
hour
vapor
exposure
in
albino
rats.
However,
the
findings
noted
for
the
58
ppm
group
animals
were
of
questionable
toxicological
significance,
in
particular
in
relation
to
human
risk.
There
was
no
exposure
concentration­
dependence
for
the
apparent
decrease
in
alveolar
macrophage
phagocytic
activity.
The
respiratory
tract
tissues
affected
in
this
group
are
known
to
be
extremely
sensitive
to
chemical
injury
in
the
rat.
Since
olfactory
epithelium
­8­
w&
417001
Hazardous
Air
Pollutant
Task
Force
covers
approximately
50%
of
the
rat's
nasal
airways,
minimal
to
mild
lesions
are
considered
unlikely
to
have
an
adverse
effect
on
function.
Laryngeal
inflammation
of
minimal
severity
is
also
unlikely
to
be
toxicologically
important.
Such
minor
effects
in
tissues
that
are
extremely
sensitive
in
the
rat
compared
to
other
species
may
be
misleading
in
estimating
human
risk.

­9­
wIIZ417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1.1.2­
Trichloroethane
(1,1.2­
TCE)
in
Rats
INTRODUCTION
The
objective
of
this
study
was
to
characterize
the
toxicological
response,
including
exposure
level­
response
relationships,
of
the
test
article
in
rats
following
a
single,
four­
hour,
whole­
body
vapor
exposure.
Anatomic
pathology
and
effects
on
body
weight,
clinical
condition
and
mortality
were
evaluated.
In
addition,
to
characterize
potential
adverse
effects
on
the
respiratory
tract,
histopathological
examination
of
fixed
tissues
and
evaluation
of
cellular
and
biochemical
parameters
in
bronchoalveolar
lavage
fluid
(BALI!)
were
performed.
Histopathological
examination
was
also
performed
for
the
liver,
kidneys,
adrenals
and
stomach.
The
inhalation
route
is
a
potential
route
for
human
exposure
to
the
test
article.

The
protocol
was
designed
and
the
study
was
conducted
in
compliance
with
the
following
guideline:
U.
S.
Environmental
Protection
Agency
Toxic
Substances
Control
Act
(EPA­
TSCA)
guideline
799.9135:
Acute
Inhalation
Toxicity
with
Histopathology.

­lO­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1
.1,2­
Trichloroethane
(1
,1,2­
TCE)
in
Rats
EXPERIMENTAL
DESIGN
The
study
consisted
of
four
groups,
each
consisting
of
10
male
and
10
female
Fischer
344
albino
rats.
Each
group
was
exposed
to
the
test
article
for
a
four­
hour
period.
For
the
mid
level
exposure
on
February
25,
2001,
the
exposure
period
was
extended
54
minutes
to
compensate
for
a
syringe
pump
failure
that
resulted
in
a
period
of
low
atmosphere
concentration.
Five
animals/
sex/
group
were
assigned
for
anatomical
pathology
evaluation
(gross
and
microscopic
examinations)
and
five
animals/
sex/
group
were
assigned
for
evaluation
of
bronchoalveolar
lavage
fluid
(BALF).
Following
exposure,
the
animals
were
maintained
for
approximately
24­
hours
prior
to
euthanasia.
Body
weights
were
determined
and
observations
for
clinical
signs
of
toxicity
were
conducted
at
the
appropriate
intervals
for
all
animals.

The
male
and
female
animals
for
anatomical
pathology
assessment
were
exposed
on
February
25,
2001.
The
animals
for
BALF
evaluation
were
exposed
on
February
26,200l
(males)
and
February
27,200l
(females).
The
target
concentration
for
the
high
exposure
level
for
the
female
BALF
evaluation
was
lowered
from
1500,
ppm
to
1000
ppm
based
on
the
death
of
three
of
five
female
rats
exposed
to
1527
ppm
on
February
25,200l.
Data
for
the
anatomical
pathology
assessment
phase
were
collected
in
computer
protocol
WIL­
417001
and
data
for
the
BALF
evaluation
phase
were
collected
in
computer
protocol
WL417001A.
The
dose
group
designations
for
the
BALI?
animals
are
presented
as
"male/
female"
ppm
on
the
report
tables.

The
following
table
summarizes
the
experimental
design:

­ll­
WlL­
417001
Hazardous
Air
Pollutant
Task
Force
Group
Number
of
Target
0
Animals
Concentration
(pnmj
Date
of
Exuosure
W&
417001
0
58
181
1527
5M,
5F
0
February
25,200
1
5M,
5F
50
February
25,200l
5M,
5F
200
February
25,200l
5M,
5F
1500
February
25,200l
WL417001A
0
60
205
1474
5M
0
February
26,200l
5M
50
February
26,200
1
5M
200
February
26,200l
5M
1500
February
26,200
1
0
5F
0
February
27,200l
45
5F
50
February
27,200l
177
5F
200
February
27,200l
840
5F
1000*
February
27,200l
M
=
Male,
F
=
Female
*
=
The
target
concentration
was
lowered
due
to
the
death
of
3/
5
females
exposed
at
1527
ppm
on
February
25,2001.

Experimental
Start
Date:
February
25,200l
Experimental
Termination
Date:
July
12,200l
­12­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
MATERIALS
AND
METHODS
Test
System:

Rat
Strain*
­*

Justification
for
Selection:

Source:

Number
on
Study:

Bodv
WeiPht
Range:

Acre
at
Start
of
Study:

Method
of
Identification:

Housing:

Quarantine:
Fischer
344
This
species
and
strain
of
animal
is
recognized
to
be
appropriate
for
acute
inhalation
studies.

Charles
River
Laboratories
401
S.
New
Hope
Road
Raleigh,
NC
27610
40
males
and
40
females.
The
females
were
nulliparous
and
non­
pregnant.

157
to
197
grams
for
males
and
114
to
137
grams
for
females
at
the
initiation
of
exposure
Approximately
eight
weeks
old
Eat­
tag
Individual
suspended
wire­
mesh
cages.
The
animals
were
maintained
by
the
animal
husbandry
staff
of
WIL
Research
Laboratories,
Inc.,
in
accordance
with
standard
operating
procedures.

The
animals
were
acclimated
to
laboratory
conditions
for
a
minimum
of
seven
days
prior
to
initiation
of
exposure.

­13­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
Test
System
(continued):

Disease
Screen:

Food
and
Water:

Environmental
Conditions:

Test
Article
Data:

Identification:

Source:
Seven
days
after
receipt,
blood
was
collected
from
the
vena
cava
of
five
animals/
sex
for
serological
screening.
Serum
samples
were
analyzed
for
antibodies
to
the
following
pathogens:
Sendai,
RCV/
SDA,
Reo,
KRV,
LCM,
H­
l,
PVM,
ROPV
viruses,
M.
puhonis
and
CAR
bacillus.
The
analyses
were
performed
by
BioReliance
Corporation,
Rockville,
Maryland
(Appendix
B).
All
individual
serological
assays
were
negative
for
all
animals
evaluated.

PMI
Nutrition
International,
Inc.
Certified
Rodent
LabDiet@
5002
and
reverse
osmosis­
treated
municipal
water
were
provided
ad
libitum.
Analysis
of
feed
was
performed
and
provided
by
the
manufacturer.
Water
was
analyzed
in
accordance
with
standard
operating
procedures.
Contaminants
were
not
present
in
feed
or
water
at
levels
expected
to
interfere
with
the
objectives
of
the
study.
Results
of
analyses
are
available
upon
sponsor
request.

Animal
room
with
controlled
temperature
[70­
74°
F
(21­
23"(
Z)],
humidity
(44­
50%)
and
light
(12
hours
light02
hours
dark).
Protocol­
specified
ranges
for
temperature
and
humidity
were
68­
76°
F
(19­
25"(
Z)
and
30­
70%,
respectively.

1,
l
,Ztrichloroethane
Dow
Chemical
2301
N.
Brazosport
Blvd.
Freeport,
TX
7754
1
Date
Received:
December
29,200O
­14­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Test
Article
Data
(continued):

Lot
Number:

Purity:

Stability:

Physical
Description:

Storage
Conditions:
NH06019301
99.56%
by
weight
based
on
the
Certificate
of
Analysis
provided
by
the
supplier
(Appendix
A).
The
purity
of
the
test
article
was
also
evaluated
by
the
WIL
Research
Laboratories,
Inc.
Analytical
Chemistry
Department
and
found
to
be
99%
(GLP­
compliant
analysis;
data
maintained
in
study
records).

Following
completion
of
animal
exposures,
the
stability
of
the
test
article
was
also
evaluated
by
the
WIL
Research
Laboratories,
Inc.
Analytical
Chemistry
Department
and
found
to
be
acceptable
(GLP­
compliant
analysis;
data
maintained
in
study
records).

Clear,
colorless
liquid
Sealed
container
at
ambient
temperature
Exposure
Methods:

The
exposures
were
conducted
in
four
500­
L
glass
and
stainless
steel
whole­
body
exposure
chambers
operated
under
dynamic
conditions
such
that
there
were
at
least
12
air
changes
per
hour.
One
chamber
was
dedicated
to
each
group.
The
animals
were
caged
individually,
without
food
and
water,
during
the
exposures.
Chamber
temperature,
relative
humidity,
ventilation
rate
and
negative
pressure
were
monitored
continuously
and
recorded
approximately
every
30
minutes
during
exposure.
A
resistance
temperature
detector
(RTD)
and
polymeric
sensors
(Model
No.
HX94C,
Omega
Engineering,
Inc.,
Stamford,
CT)
provided
monitoring
of
temperature
and
humidity,
respectively.
Chamber
ventilation
rate
was
measured
by
a
sharp­
edge
orifice
meter
and
pressure
gauges
(Dwyer,
Inc.,
Michigan
City,
IN)
calibrated
to
convert
pressure
to
airflow
rate
in
liters
per
minute.
Oxygen
content
of
the
exposure
atmosphere
was
measured
during
method
development
prior
to
study
initiation
by
a
Mine
Safety
Appliances
(Pittsburgh,
PA)
Remote
Sampling
System
equipped
with
an
oxygen
cell.
The
measured
oxygen
content
was
greater
than
19%.

­15
WrL­
417001
Hazardous
Air
Pollutant
Task
Force
Test
Atmosphere
Generation
Methods:

Vapor
atmospheres
of
the
test
article
were
generated
using
systems
similar
to
those
shown
in
Figures
1
and
2.
The
test
article
was
delivered
at
a
known
rate
to
the
top
of
a
glass
vaporization
column
(Ace
Glass,
Inc.,
Vineland,
NJ)
packed
with
various­
sized
glass
beads.
A
syringe
pump
(Harvard
Apparatus,
Holliston,
PA)
and
appropriately
sized
glass
syringe
were
used
to
deliver
the
test
article
for
the
low
and
mid
exposure
concentrations.
During
the
mid
level
exposure
on
February
25,2001,
a
syringe
pump
failure
resulted
in
a
period
of
low
atmosphere
concentration.
To
compensate
for
this
excursion,
the
exposure
duration
was
extended
to
4
hours
and
54
minutes.
A
liquid
metering
pump
(Fluid
Metering
Inc.,
Oyster
Bay,
NY)
was
used
for
the
high
exposure
concentration.
Compressed
air
at
a
known
flow
rate
was
metered
to
the
bottom
of
the
vaporization
column.
Vaporization
occurred
as
the
test
article
flowed
down
through
the
beads
while
the
compressed
air
flowed
up
through
the
column.
Each
vaporization
column
was
wrapped
with
a
flexible
electric
heating
tape
(Omega
Engineering,
Inc.)
set
at
approximately
75°
C
to
facilitate
vaporization
of
the
test
article.
The
resulting
vapor
was
piped
to
the
exposure
chamber
inlet
where
the
vapor
was
diluted
to
the
desired
exposure
concentration
by
chamber
supply
air.
The
control
group
received
filtered
air.
Operational
conditions
for
the
generation
system
are
provided
in
the
following
table:

­16­
wlL417001
Hazardous
Air
Pollutant
Task
Force
Group
Syringe
Size
Test
Article
Compressed
Air
Chamber
or
Lab
Flow
Rate
Flow
Rate
Ventilation
liIldh@
i3eaQ
Rate
WIL­
417001
0
58
181
1527
WL417001A­
Males
0
60
205
1474
WIL­
417001A­
Females
0
45
177
840
NA
NA
16
103
20
cc
0.05
17
99­
119
50
cc
0.16­
0.20
17
119­
122
QG20
l/
S"
1.01
14
94­
102
NA
NA
16
103
20
cc
0.05
17
114­
126
50
cc
0.16
17
121
QG20
l/
S"
1.01
14
97­
102
NA
NA
16
103
20
cc
0.05
17
112­
126
50
cc
0.16­
0.24
17
115­
121
QG20
l/
S"
0.68­
1.19
14
96­
101
Methods
of
Characterization
of
Exnosure
Atmospheres:

Nominal
Concentration:

Nominal
exposure
concentrations
were
calculated
as
the
total
weight
of
test
article
used
during
each
exposure
divided
by
the
total
volume
of
air
that
passed
through
the
system
during
the
exposure.

Actual
Concentrations:

Actual
exposure
concentrations
were
measured
using
gas
chromatography.
A
pump
and
multi­
port
sampling
valve
drew
samples
of
the
exposure
atmospheres
to
the
gas
chromatograph
(GC).
The
following
table
summarizes
the
GC
conditions:

­17­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Instrument:
Detector:
Column:

Gases
Carrier­
Helium
Fuel­
Hydrogen
Air
Temperatures
("
C)
Detector:
Column:
Injector:
Injection
Volume
Retention
Time:
Hewlett
Packard
5890
Series
II
Flame
ionization
DB­
WAX
0.25
p
film
thickness130
m
x
0.25
mm
80
psig
(30
ml/
mm)
16
psig
(30
ml/
mm)
35
psig
(300
ml/
min)

250
160
250
0.25
ml
Approximately
0.77
minutes
The
GC
was
standardized
using
known
concentrations
of
the
test
article
prepared
in
Tedlar@
gas
bags
(SK,
Inc.,
Eighty
Four,
PA).
Each
standard
was
prepared
by
injecting
a
known
volume
of
test
article
into
a
glass
vaporization
bulb.
A
continuous
flow
of
air
through
the
bulb
carried
the
vaporized
test
article
to
a
40­
L
bag.
A
dry
test
meter
(American
Meter
Co.,
Horsham,
PA)
measured
the
volume
of
air
delivered
to
the
bag.
Concentrations
of
the
gas­
phase
standards
were
calculated
as
follows:

Concentration
(ppm)
=
VOL
x
R
x
T
x
D
x
10m3
x
1
Q
6
LxGMWxP
Where:
VOL
=
Volume
of
the
test
article,
~1
R=
Universal
gas
constant,
62.36
L
mmHg/
mol
K
T=
Nominal
laboratory
temperature,
294
K
D=
Density
of
test
article,
1.4350
g/
ml
L=
Volume
of
air
used
to
make
bag,
30
L
GMW=
Molecular
weight
of
test
article,
133.41
P=
Laboratory
barometric
pressure,
730
mmHg
1(
r3
=
p.
l
to
ml
conversion
106=
ppm
conversion
Standards
were
prepared
that
bracketed
the
target
concentration
for
each
exposure.
Each
standard
bag
was
prepared
and
analyzed
on
the
GC.
An
instrument
response
for
each
standard
was
recorded
to
produce
a
calibration
curve.
On
the
day
of
exposure,
one
standard
was
analyzed
to
confirm
GC
calibration.
Then
for
each
exposure,
the
instrument
response
was
converted
to
concentration
from
the
calibration
curve
for
each
sample.

­18­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Temporal
Stability
and
Homogeneity
of
the
Test
Atmosphere
Homogeneity
of
the
exposure
atmospheres
was
determined
during
the
method
development
phase
of
the
study.
Samples
were
taken
and
compared
from
two
remote
locations
and
a
reference
position.
The
remote
samples
were
taken
from
the
back
upper
right
and
front
lower
left
comers.
The
reference
samples
were
collected
from
the
approximate
center
of
the
chamber.
The
determinations
were
performed
in
triplicate
and
a
mean
percent
difference
from
the
reference
position
was
calculated
for
each
remote
location.
Mean
concentrations
collected
at
the
remote
locations
were
within
+
10%
of
the
reference
location
and
considered
homogeneous
for
the
purposes
of
this
study.
In
addition,
the
stability
of
the
test
article
concentrations
over
time
was
found
to
be
acceptable
during
method
development
trial
exposures.

Chemical
Puritv
of
the
Test
Atmosuhere
During
the
1474
ppm
animal
exposure,
the
chemical
purity
of
the
test
atmosphere
was
analyzed
by
the
following
procedure.
A
sample
of
the
test
atmosphere
was
analyzed
by
the
gas
chromatograph.
The
GC
was
optimized
and
the
run
length
was
extended
to
allow
for
detection
of
all
test
article­
associated
peaks.
The
high
concentration
standard
(1801
ppm)
was
then
analyzed
in
the
same
manner.
The
percent
of
total
peak
area
counts
represented
by
the
area
counts
for
the
atmosphere
and
standard
peaks
were
calculated
and
compared.
The
1,1,2­
TCE
peak
represented
99.5%
and
99.4%
of
all
peaks
detected
for
the
test
atmosphere
and
1801
ppm
standard,
respectively.

Absence
of
Aerosol
in
the
Test
Atmosnhere
During
the
1474
ppm
animal
exposure,
the
test
atmosphere
was
evaluated
for
the
absence
of
aerosol
using
a
light
scattering
aerosol
photometer
(MIE,
Inc.
Billerica,
MA).
The
photometer
was
connected
to
a
strip
chart
recorder
(Omega
Engineering,
Inc.).
The
photometer
was
first
standardized
with
a
room
air
sample.
A
sample
of
the
test
atmosphere
was
then
pulled
through
the
photometer
and
the
output
was
recorded.
No
aerosols
were
detected
during
this
evaluation.

­19­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
OBSERVATIONS
Mortality:

Each
rat
was
observed
for
mortality/
moribundity
twice
daily
during
the
acclimation
period,
between
3
and
4
p.
m.
on
the
day
of
exposure
and
before
8
a.
m.
on
the
day
following
exposure.

Detailed
Phvsical
Examinations:

Detailed
physical
examinations
were
performed
approximately
one
week
prior
to
the
initiation
of
exposures,
on
the
day
of
animals
were
assigned
to
groups
and
on
the
day
following
exposure
(prior
to
necropsy).
The
animals
were
removed
from
their
home
cages
and
placed
in
a
standard
arena
for
observation.
Observations
were
detailed
and
carefully
noted.
Signs
noted
included,
but
were
not
limited
to,
changes
in
skin,
fur,
eyes,
mucous
membranes,
occurrence
of
secretions
and
excretions,
and
autonomic
activity
(e.
g.,
lacrimation,
piloerection,
pupil
size,
unusual
respiratory
pattern).
Changes
in
gait,
posture
and
response
to
handling,
as
well
as
the
presence
of
clonic
or
tonic
movements,
stereotypes
(e.
g.,
excessive
grooming,
repetitive
circling)
or
bizarre
behavior
(e.
g.,
self­
mutilation,
walking
backwards)
were
recorded.
The
absence
or
presence
of
findings
were
recorded
for
individual
animals.
If
applicable,
animals
to
be
eutbanized
prior
to
scheduled
necropsy
received
an
additional
detailed
physical
examination
prior
to
euthanasia
to
assist
in
determination
of
the
cause
of
death.
Pretest
clinical
observations
are
presented
in
Appendix
C.

Clinical
Observations:

Each
rat
was
observed
immediately
following
exposure
for
clinical
signs.
Observations
included,
but
were
not
limited
to,
changes
in
the
skin,
fur,
eyes
and
mucous
membranes,
respiratory,
circulatory,
autonomic
and
central
nervous
system
functions,
somatomotor
activity
and
behavior
patterns.
Findings
noted
at
the
clinical
examination
were
recorded
for
individual
animals.

At
the
approximate
midpoint
of
each
exposure,
visible
animals
were
observed
for
response
to
a
novel
stimulus
to
determine
their
state
of
arousal.
The
stimulus
was
produced
by
allowing
a
striker
(PVC
coupling)
to
contact
the
glass
of
the
exposure
chamber
door.
The
coupling
was
attached
to
a
length
of
cotton
rope
that
was
held
against
the
glass
at
approximately
10
cm
above
the
level
of
the
cage
rack.
The
coupling
was
raised
until
the
rope
was
approximately
perpendicular
to
the
glass
and
then
released.
The
response
of
each
visible
animal
was
recorded
as
1)
no
reaction,
2)
slight
reaction,
3)
notably
increased
reaction.

­2o­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Body
Weights:

Body
weights
were
obtained
twice
during
the
acclimation
period,
prior
to
exposure
and
prior
to
necropsy
on
the
day
following
exposure.

Necropsy:

Animals
were
euthanized
approximately
24
hours
following
exposure
and
underwent
a
gross
necropsy.
In
addition,
complete
necropsies
were
performed
on
the
animals
that
were
found
dead.
Animals
were
euthanized
by
isoflurane
anesthesia
followed
by
exsanguinated.
At
the
time
of
necropsy,
the
following
tissues
and
organs
were
collected
and
preserved
in
10%
neutral
buffered
formalin
or
processed
as
noted:

Adrenals
(2)
Aorta
Bone
with
marrow
(stemebrae)
Brain
Forebrain
Midbrain
Hindbrain
Epididymides
(2)
'
Eyes
with
optic
nerve
(2)
a
Gastrointestinal
tract
Esophagus
Stomach
Duodenum
Jejunum
Ileum
Cecum
Colon
Rectum
Heart
Kidneys
(2)
Larynx
Liver
(sections
of
two
lobes)
Lungs
(including
bronchi,
fixed
by
inflation
with
fixative
at
a
constant
pressure)

a
=
Placed
in
Davidson's
solution
Lymph
node
Mediastinal
Tracheobronchial
Nasal
Tissues
Ovaries
with
oviducts
(2)
Pancreas
Parathyroidsb
Peripheral
nerve
(sciatic)
Pituitary
Prostate
Salivary
glands
[mandibular
(2)]
Seminal
vesicles
(2)
Skeletal
muscle
(rectus
femoris)
Skin
Spinal
cord
Cervical
Thoracic
Lumbar
Spleen
Testes
(2)
'
Thymus
Thyroids
Trachea
Urinary
bladder
Uterus
with
vagina
Gross
lesions
(when
possible)

b
=
Examined
if
in
the
plane
of
section
or
when
a
gross
lesion
was
present
c
=
Placed
in
Bouin's
solution
­21­
WrL­
417001
Hazardous
Air
Pollutant
Task
Force
Organ
Weights
The
following
organs
were
weighed
from
the
animals
at
the
scheduled
necropsy.

Adrenals
Brain
Kidneys
Liver
Lungs
(prior
to
inflation
with
fixative)
Ovaries
Testes
Paired
organs
were
weighed
together.
Organ
to
final
body
weight
ratios
were
calculated.

Microsconic
Examination
After
fixation,
protocol­
specified
tissues
were
trimmed
according
to
the
standard
operating
procedures
of
WIL
Research
Laboratories,
Inc.
Trimmed
specimens
were
processed
into
paraffin
blocks,
sectioned
at
five
to
eight
microns,
placed
on
glass
microscope
slides
and
stained
with
hematoxylin
and
eosin.

Following
collection
of
the
specified
tissues,
the
entire
head
was
removed
and
decalcified.
Six
cross­
sections
of
the
nasal
tissues
were
prepared
for
microscopic
examination
in
accordance
with
the
method
described
by
Morgan'.
This
method
provides
optimal
evaluation
of
the
nasopharyngeal
meatus
and
exceeds
the
requirements
of
the
799.9135
guideline.
The
larynx
was
sectioned
to
include
epithelium
covering
the
base
of
the
epiglottis,
the
ventral
pouch
and
the
medial
surfaces
of
the
vocal
processes
of
the
arytenoid
cartilages.

Microscopic
examination
of
the
liver,
kidneys,
stomach
and
tissues
of
the
respiratory
tract
was
conducted
for
all
animals
in
the
control
and
high
exposure
groups.
The
respiratory
tract
tissues
examined
were
the
nasal
tissues,
larynx,
trachea,
bronchi
and
lungs,
including
airways,
terminal
and
respiratory
bronchioles,
alveolar
ducts
and
sacs
and
interstitial
tissues.
Nasal
sections
II­
VI,
adrenal
glands,
kidneys,
larynx,
liver
and
stomach
(non­
glandular
to
proximal
duodenum)
were
examined
microscopically
from
all
animals
in
the
low
and
mid
exposure
groups
based
on
findings
in
the
high
exposure
groups,
on
the
findings
noted
for
gross
lesions
and/
or
based
on
organ
weights.

Microscopic
examination
was
conducted
by
Gary
Marit,
D.
V.
M.,
D.
A.
C.
V.
P.,
WIL
Research
Laboratories,
Inc.

­22­
WJL­
417001
Hazardous
Air
Pollutant
Task
Force
Bronchoalveolar
Lavage
Evaluation
Approximately
24
hours
following
exposure,
surviving
animals
scheduled
for
evaluation
of
bronchoalveolar
lavage
fluid
(BALF)
were
euthanized
by
isoflurane
and
thoroughly
exsanguinated.
The
lungs
were
examined
macroscopically
and
any
lesions
were
noted.
To
promote
maximum
drainage
of
blood
from
the
lungs,
the
animals
were
anesthetized,
but
not
allowed
to
die
prior
to
initiation
of
exsanguination.
After
the
diaphragm
was
punctured
to
allow
the
lungs
to
collapse,
the
thoracic
cavity
was
carefully
opened
from
the
diaphragm
to
the
larynx.
A
small
hole
was
then
cut
into
the
trachea
just
below
the
larynx
and
an
appropriately
sized
ball­
tipped
dosing
cannula
was
tied
in
place.
Each
animal
was
lavaged
six
times
using
Hank's
Balanced
Salt
Solution
without
calcium,
magnesium
and
phenol
red.
The
lavage
solution
was
warmed
to
37
­+
4°
C
prior
to
use.
The
individual
lavage
volume
was
5
ml
for
males
and
4
ml
for
females.

Recovered
lavage
fluids
were
placed
in
15ml
(1"
lavage)
and
50­
ml
(lavages
2­
6)
polypropylene
centrifuge
tubes.
The
tubes
were
centrifuged
for
10
minutes
at
2500
RPM
in
a
refrigerated
dentrifuge
(2­
8°
C).
Using
aseptic
met,
hods,
the
supematant
from
the
tubes
was
decanted
into
labeled
storage
tubes.
The
supematant
from
the
first
tube
was
stored
on
ice
until
analyzed
for
total
protein.
The
cell
pellets
were
resuspended
in
4.5
ml
of
resuspension
media
(RPMI
1640
with
10%
Fetal
Calf
Serum)
using
a
vortex
mixer
and
then
combined.
The
total
volume
of
the
cell
suspension
was
recorded.
For
total
white
blood
cell
count
(cells/
ml
BALF)
and
preparation
of
slides
for
differential
white
blood
cell
count,
approximately
0.5­
m]
of
the
suspension
was
transferred
to
a
separate
storage
tube.
Total
protein
analysis
and
white
blood
cell
counts
were
performed
by
WIL
Research
Laboratories,
Inc.
Clinical
pathology
methods
are
presented
in
Appendix
D.

Slides
for
differential
count
were
prepared
and
shipped
to
Gail
L.
Walter,
D.
V.
M.,
D.
A.
C.
V.
P.,
D.
A.
B.
T,
Clinical
Pathology
Consultant,
Kalamazoo,
MI,
for
evaluation
(Appendix
E).
For
each
differential
count,
200
or
201
nucleated
cells
were
counted
and
identified
microscopically
as
large
mononuclear
cells,
lymphocytes,
neutrophils,
eosinophils,
epithelial
cells
or
mitotic
cells.

The
remaining
cell
suspension
was
used
to
evaluate
the
effects
of
test
article
exposure
on
alveolar
macrophage
function.
Phagocytosis
and
respiratory
burst
assays
were
performed
using
the
methods
of
Burleson'
and
Rodgers3,
respectively.
The
assay
samples
were
shipped
on
blue
ice
via
overnight
courier
to
Gary
R.
Burleson,
Burleson
Research
Technologies,
Raleigh,
NC,
for
analysis.
The
methods
and
results
of
the
analyses
are
presented
in
Appendix
F.
All
Quality
Assurance
functions
for
these
analyses
were
the
responsibility
of
Burleson
Research
Technologies.

­23­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
STATISTICAL
METHODS
All
analyses
were
conducted
using
two­
tailed
tests
for
significance
levels
of
1%
and
5%
comparing
the
test
article­
treated
groups
to
the
air
control
group
by
sex.
All
means
were
presented
with
standard
deviations
(S.
D.)
and
the
numbers
of
sampling
units
(N)
used
to
calculate
the
means.
Statistical
analyses
were
not
conducted
if
the
number
of
animals
was
two
or
less.
All
statistical
tests
were
performed
using
appropriate
computing
devices
or
programs.
Body
weight,
body
weight
change,
BALF
values
and
absolute
and
relative
organ
weight
data
were
subjected
to
a
one­
way
analysis
of
variance
(ANOVA)
followed
by
Dunnett's
test',
if
the
ANOVA
revealed
statistical
significance
(~~
0.05).

­24­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
RESULTS
Characterization
of
Exnosure
Atmosnheres:

Environmental
Conditions:

Mean
temperature
and
relative
humidity
of
the
exposure
atmosphere
are
summarized
in
the
following
table:

Group
!ilzr2@

WIL­
417001
0
58
181
1527
Temperature
C°
Cj
Relative
Humidity
(%)
w&
Q
@
Mean
S.
D.
@

21
0.4
8
47
1.2
8
21
0.5
8
49
2.1
8
24
0.3
9
41
1.6
9
22
0.5
8
46
1.7
8
WIL­
4
1700
1
A­
Males
0
21
0.5
8
43
1.2
8
60
21
0.5
8
44
1.6
8
205
23
0.5
a
40
1.1
8
1474
21
0.0
8
42
1.1
8
WIL­
417001A­
Females
0
21
0.5
8
43
2.3
8
45
21
0.5
8
46
1.8
8
177
23
0.5
8
41
1.8
8
840
21
0.0
9
43
1.9
9
Actual
Concentrations
(Table
1):

The
following
table
summarizes
the
actual
exposure
concentration
data
for
the
exposure:

­25
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Group
0
wIL­
417001
0
58
181
1527
WL417OOlA­
Males
0
60
205
1474
WIL­
417001A­
Females
0
45
177
840
Mean
Standard
0
0.0
6
58
18.4
8
181
91.4
10
1527
33.0
6
0
0.0
6
60
6.6
6
205
5.3
6
1474
60.1
6
0
0.0
7
45
6.3
7
177
48.1
8
840
185.2
9
Number
of
SamDIes
The
calculated
tss
equilibration
time
was
approximately
18
to
23
minutes
for
all
exposures.
To
compensate
for
the
equilibration
time,
the
animals
were
left
in
the
chambers
for
approximately
20
minutes
following
shutdown
of
the
generation
system
at
the
end
of
exposure.
It
should
be
noted
that
the
mid
level
exposure
on
February
25,
2001
(mean
concentration
of
18
1
ppm)
was
extended
54
minutes
to
compensate
for
a
period
of
low
concentrations
caused
by
a
syringe
pump
failure.
It
can
be
estimated
that
the
mean
exposure
concentration
was
at
least
200
ppm
(the
target
exposure
level)
for
approximately
a
four­
hour
period.

Mortalitv
(Tables
2
and
14):

Three
females
in
the
`1527
ppm
group
were
found
dead
on
the
day
following
exposure.
Three
females
in
the
840
ppm
bronchoalveolar
lavage
(BAL)
group
died
following
exposure.
For
these
three
females,
one
was
found
dead
immediately
following
exposure
and
one
was
found
dead
and
one
was
euthanized
in
extremis
on
the
day
following
exposure.
Rush
(emergency)
bronchoalveolar
lavage
procedures
were
performed
for
the
two
female
rats
that
died
or
were
euthanized
on
the
day
following
exposure
(840
ppm
BAL
group).
There
were
no
other
deaths
during
the
study.

­26­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Clinical
Observations
(Tables
2,3,
14
and
151:

Immediately
following
exposure,
all
animals
in
the
1527
ppm
group
appeared
to
be
sleeping,
had
decreased
respiration
rates
and
clear
discharge
of
the
eyes.
Similarly,
males
in
the
1474
ppm
BAL
group
and
females
in
the
840
ppm
BAL
group
appeared
to
be
sleeping,
were
lethargic
and/
or
ataxic
after
exposure.
Decreased
respiration
rates
were
also
observed
for
one
male
and
four
females
in
these
high
concentration
BAL
groups.
Clear
discharge
of
the
eyes
was
noted
for
two
males
and
four
females
in
the
1474
and
840
ppm
groups,
respectively,
immediately
following
exposure.
There
were
no
other
toxicologically
significant
clinical
observations
immediately
following
exposure.

On
the
day
following
exposure,
clinical
observations
were
again
limited
to
animals
in
the
high
concentration
toxicology/
pathology
and
BAL
groups.
Lethargy
was
observed
for
8/
10
males
in
the
1527
and
1474
ppm
groups
combined.
Hypothermia
(body
cool
to
the
touch)
was
observed
for
four
males
in
the
1474
ppm
BAL
group.
Decreased
respiration
rate
was
noted
for
three
males
in
the
1527
ppm
group
and
for
one
male
in
the
1474
ppm
group.
In
addition,
reddish
brown
urine
was
noted
for
3/
10
males
in
the
high
concentration
groups
on
the
day
following
exposure.
Clinical
observations
in
the
1527
ppm
group
females
were
clear
discharge
of
the
eye(
s),
decreased
respiration
rate,
hypothermia
and
lethargy.
Ataxia,
clear
discharge
of
the
eye(
s),
decreased
respiration
rate,
hypothermia,
lethargy
and
prostration
were
observed
for
females
in
the
840
ppm
group.
There
were
no
other
toxicologically
significant
clinical
observations
on
the
day
following
exposure.

Response
to
Novel
Stimulus
(Tables
4
and
16):

The
response
to
novel
stimulus
data
(from
the
response
induced
by
a
sudden
noise)
collected
at
the
approximate
midpoint
of
each
exposure
indicated
a
definite
decrease
in
the
state
of
arousal
in
the
high
concentration
groups
(1527,
1474
and
840
ppm)
when
compared
to
the
control
group.
The
responses
in
the
low
and
mid
exposure
level
groups
included
a
mix
of
reactions
(none,
slight
and
increased)
and
were
considered
normal
when
compared
to
the
control
group.

Body
Weights
(Tables
5,6,
17
and
18):

Mean
body
weight
gains
in
the
1527
ppm
group
males
and
females
and
in
the
1474
ppm
group
males
and
840
ppm
group
females
were
significantly
(~~
0.01)
lower
than
the
control
group
on
the
day
following
exposure
(day
1)
when
compared
to
the
control
group.
As
a
result,
mean
body
weights
were
significantly
(~~
0.5)
lower
than
the
control
group
in
the
1527
ppm
group
males
and
females
and
1474
ppm
group
males
on
day
1.
There
were
no
other
remarkable
changes
in
body
weight
data.

­27­
WIL­
41700
1
Hazardous
Air
Pollutant
Task
Force
Bronchoalveolar
Lavage
Evaluations
(Tables
7
and
19;
Appendices
E
and
F):

Mean
total
protein
was
significantly
(~~
0.05
or
~~
0.01)
higher
than
the
control
group
in
the
bronchoalveolar
lavage
fluid
of
the
1474
ppm
group
males
and
840
ppm
group
females.
Relative
to
the
control
group
means,
the
mean
total
protein
level
was
increased
167%
and
55%
in
the
high
concentration
male
and
female
groups,
respectively.
No
alterations
in
BALF
total
or
differential
cell
counts
attributed
to
test
article
exposure
were
observed.

The
test
guideline
for
this
acute
study
requires
evaluation
of
alveolar
macrophage
function
of
cells
collected
by
bronchoalveolar
lavage.
Two
assays
were
used
to
evaluate
macrophage
function
in
this
study.
Measurement
of
functional
phagocytic
activity,
by
quantifying
fluorescence
intensity
produced
by
intracellular
(i.
e.,
phagocytized)
fluorescein­
labeled
Escherichia
coli,
revealed
a
decrease
in
phagocytic
activity
for
male
rats
exposed
to
60,
205
or
1474
ppm
1,1,2­
TCE
(compared
to
air
exposed
controls).
However,
for
female
rats,
phagocytic
activity
appeared
to
be
decreased
in
the
mid­
concentration
(177
ppm)
exposure
group
only.
It
is
noteworthy
that
there
was
no
exposure
concentration­
relationship
for
the
apparent
effects
on
phagocytic
activity
in
males.
The
magnitude
of
the
effect
was
similar
following
exposure
to
either
60
or
1474
ppm
and
the
most
pronounced
effect
was
found
in
the
mid­
concentration
exposure
group.
In
addition,
historical
control
data,
which
would
provide
normal
or
typical
values
for
phagocytic
activity
of
alveolar
macrophages
from
Fischer
344
rats
(determined
using
the
same
assay
method),
is
not
available.
The
toxicological
significance
of
the
changes
observed
in
this
study
is
uncertain
based
on
the
lack
of
exposure
concentration­
dependence
and
the
unavailability
of
historical
control
data.
The
respiratory
burst
is
a
metabolic
response
of
phagocytic
cells
that
is
associated
with
microbicidal
activity.
Exposure
to
1,1,2­
trichloroethane
by
inhalation
had
no
effect
on
respiratory
burst
activity
(neither
stimulated
nor
non­
stimulated)
by
alveolar
macrophages.

Necropsy
(Tables
8,9,20
and
21):

A
dark
red
area
in
the
glandular
portion
of
the
stomach
was
observed
for
one
female
in
the
1527
ppm
group
that
died
early.
There
were
no
other
internal
macroscopic
findings
for
the
animals
that
died
prior
to
the
scheduled
necropsy.

At
the
scheduled
necropsy,
dark
red
area(
s)
on
the
liver
were
observed
for
two
males
and
a
pale
liver
was
observed
for
one
female
in
the
1527
ppm
group.
Dark
red
areas
in
the
glandular
and/
or
non­
glandular
portions
of
the
stomach
were
observed
for
one
male
in
the
1527
ppm
group
at
the
scheduled
necropsy.
Dark
red
contents
in
the
cecum,
ileum,
jejunum
and/
or
stomach
were
observed
for
four
males
and
one
female
in
the
1527
ppm
group.
Dark
red
contents
in
the
urinary
bladder
were
also
observed
for
one
male
in
the
­28­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
1527
ppm
group.
There
were
no
other
internal
macroscopic
findings
at
the
scheduled
necropsy.

Organ
Weights
(Tables
10,
11,22
and
23):

Mean
relative
(to
final
body
weight)
kidney
weight
in
the
1527
ppm
group
males
was
significantly
(~~
0.01)
higher
than
the
control
group.
The
increase
correlated
with
the
microscopic
findings
noted
for
the
kidney.
There
were
no
other
test
article­
related
effects
on
organ
weights.
Mean
absolute
and
relative
(to
final
body
weight)
adrenal
gland
weights
in
the
1527
ppm
group
males
were
significantly
(~~
0.01)
higher
than
the
control
group.
However,
microscopic
examination
of
the
adrenal
glands
failed
to
reveal
a
cause
for
the
increased
weights.
The
change
in
adrenal
gland
weight
may
have
been
caused
by
stress.
Mean
absolute
and
relative
lung
weights
in
the
181
ppm
group
females
were
significantly
(p&
O5
or
~~
0.01)
lower
than
the
control
group
values.
However,
since
a
dose­
related
manner
change
was
not
observed,
the
decreases
in
lung
weights
were
not
considered
a
result
of
1,1,2­
TCE
exposure.
Mean
relative
brain
weight
was
increased
(~~
0.01)
in
the
1527
ppm
group
males.
However,
the
increase
was
slight
and
was
most
likely
due
to
the
decrease
in
mean
body
weight
observed
for
this
group.
There
were
no
other
remarkable
changes
in
organ
weight
data.

Microsconic
Examination
(Tables
12,
13,20
and
21):

Test
article­
related
changes
were
seen
in
the
liver,
nasal
cavity,
larynx,
kidney
and
stomach.
In
the
liver,
moderate
to
severe
hepatocellular
necrosis
was
seen
in
all
animals
in
the
1527
ppm
group.
Minimal
to
mild
centrilobular
necrosis
was
observed
for
4/
5
females
in
the
181
ppm
group.
Centrilobular
necrosis
was
not
observed
in
the
181
ppm
group
males.
The
following
table
summarizes
the
results
for
hepatocellular
centrilobular
necrosis:

In
the
nasal
cavity,
test
article­
related
changes
involved
primarily
the
olfactory
epithelium
and
to
a
lesser
extent,
the
transitional
epithelium.
The
affected
olfactory
epithelium
was
­29­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
generally
present
in
nasal
regions
III­
VI.
Minimal
to
severe
necrosis
was
observed
in
multiple
sections
of
all
181
and
1527
ppm
group
males
and
females
with
severity
tending
to
increase
with
exposure
level
and
proximity
to
the
nares.
At
58
ppm,
all
females
and
2/
S
males
had
minimal
to
mild
olfactory
epithelial
necrosis
in
at
least
one
nasal
section.
Necrosis
was
generally
limited
to
the
dorsal
meatus
and
adjacent
septum
and
ethymoid
turbinates.
Olfactory
epithelial
necrosis
was
not
observed
in
the
control
group.
The
following
table
summarizes
the
necrosis
of
olfactory
epithelium:

Severity
Grade
0
ppm
58
ppm
181
ppm
1527
ppm
Necrosis,
Olfactory
Epitheiium
Nasal
Level
III
Males
Total
n
0
4
5
I
Minimal
NA
NA
1
1
Females
,
I"
z­%
,
L
I
1
I
Mild
NA
1
NA
1
2
1
Moderate
NA
I
I\
rn
I
1
I
t
Severe
NA
)
NA
1
0
I
2
Total
0
I
1
Minimal
NA
;
I
1
;
E
;
Mild
NA
n
7
n
_.__
1
Modera!
:e
NA
0
6
2
,
PP.,
P,,
U~"
c.
IG
NA
0
0
3
I
I­
rr+
nl
n
7
c
z
.
.­­­
I
Level
IV
h,
rmlnr
1
Nasal
1
"Ia*

Minimal
iA
;
;,
J
1
Mild
NA
0
5
3
Moderate
NA
0
0
1
Total
0
5
5
5
Minimal
NA
3
n
n
J
,
1
1
1
Mild
1
NA
1
4
4
In
the
transitional
epithelium
of
the
nose,
single
cell
necrosis
was
seen
on
the
turbinates
and
lateral
wall
of
nasal
sections
II
and,
variably,
III
from
all
males
and
females
in
the
1527
ppm
group.
Minimal
single
cell
necrosis
was
seen
in
one
control
group
male
and
one
181
ppm
group
female.

­3o­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
In
the
larynx,
acute
inflammation
(minimal
to
mild)
was
observed
in
all
females
and
3/
5
males
in
the
1527
ppm
group.
Four
of
five
males
in
the
58
ppm
group
and
2/
5
males
in
the
181
ppm
group
also
had
minimal
laryngeal
inflammation.
It
was
unclear
why
the
highest
incidence
of
this
change
was
observed
in
the
lowest
concentration
group.
In
females,
acute
inflammation
was
seen
in
l/
5
animals
in
the
control
and
181
ppm
group,
suggesting
a
no­
observe­
effect
level
(NOEL)
of
181
ppm
in
females.
However,
there
were
animal
to
animal
variations
in
the
laryngeal
sections
and
not
every
desired
landmark
was
well
represented.
The
ventral
pouch
was
the
site
most
commonly
affected
with
inflammation
in
treated
rats.

In
the
kidney,
a
small
number
of
cortical
tubules
contained
a
brightly
eosinophilic
to
basophilic
coarsely
granular
material.
The
location
of
this
luminal
debris
was
nonspecific
and
the
composition
and
significance
of
this
change
was
unclear.
Luminal
debris
was
seen
only
in
rats
exposed
to
concentrations
of
1527
ppm
(l/
5
males,
315
females).
In
the
glandular
stomach,
ulceration
and/
or
erosion
was
seen
in
3/
5
males
and
2/
5
females
exposed
at
1527
ppm.
This
change
was
not
seen
in
any
other
groups
and
was
the
most
likely
source
of
the
dark
red
areas
and
contents
seen
in
the
stomach
at
necropsy.
This
change
may
be
secondary
to
stress
and
not
a
direct
effect
of
l,
l,
ZTCE
exposure.
No
other
test
article­
related
microscopic
changes
were
seen.
All
other
observations
were
considered
spontaneous
and/
or
incidental,
and
were
unrelated
to
the
administration
of
1,1,2­
TCE.

­31­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
CONCLUSION
Three
females
in
the
1527
ppm
group
and
three
females
in
the
840
ppm
group
were
found
dead
or
euthanized
in
extremis
as
a
result
of
test
article
exposure.
There
were
no
other
deaths
during
the
study.

Animals
in
the
high
concentration
groups
(1527,
1474
and
840
ppm)
showed
no
reaction
to
novel
stimulus
(a
sudden
noise)
at
the
approximate
midpoint
of
exposure.
All
other
test
article
groups
reacted
similarly
to
the
control
group
and
were
considered
normal.
Immediately
following
exposure,
all
animals
in
the
high
concentration
groups
(1527
ppm
and
1474
or
840
ppm)
appeared
to
be
sleeping,
were
lethargic,
ataxic,
had
decreased
respiration
rates
and/
or
clear
discharge
of
the
eyes.
These
clinical
effects
observed
for
high
concentration
animals
during
or
immediately
following
exposure
were
primarily
associated
with
anesthetic
or
narcotic
effects
of
the
test
article,
a
chlorinated,
short­
chain
hydrocarbon.
On
the
day
following
exposure,
ataxia,
decreased
respiration
rate,
lethargy,
hypothermia,
reddish
brown
urine
and/
or
clear
discharge
of
the
eye(
s)
were
observed
in
the
high
concentration
groups.
There
were
no
other
toxicologically
significant
clinical
observations
immediately
following
or
on
the
day
following
exposure.

Test
article­
related
body
weight
effects
were
also
limited
to
the
high
concentration
groups.
Decreased
mean
body
weight
gains
were
observed
in
the
1527
ppm
group
males
and
females
and
in
the
1474
ppm
group
males
and
840
ppm
group
females
on
the
day
following
exposure
(day
1)
when
compared
to
the
control
group.
As
a
result,
mean
body
weights
were
decreased
in
the
1527
ppm
group
males
and
females
and
1474
ppm
group
males
on
day
1.
There
were
no
other
remarkable
changes
in
body
weight
data.

Mean
total
protein
was
increased
in
the
bronchoalveolar
lavage
fluid
of
the
1474
ppm
group
males
and
840
ppm
group
females.
No
microscopic
findings
in
the
lung
correlated
with
this
increase
in
lavage
fluid
total
protein.
Additionally,
no
alterations
in
BALF
total
or
differential
cell
counts
attributed
to
test
article
exposure
were
observed
at
any
concentration.
`The
functional
capacity
of
alveolar
macrophages
isolated
from
bronchoalveolar
lavage
fluid
was
evaluated.
Phagocytic
activity
was
reduced
in
all
male
1,1,2­
TCE
exposure
groups
with
the
greatest
effect
in
the
mid­
concentration
group.
For
females,
phagocytic
activity
appeared
to
be
decreased
only
in
the
mid­
concentration
group.
It
is
noteworthy
that
there
was
no
exposure
concentration­
dependence
for
the
apparent
effects
on
phagocytic
activity
in
males.
In
addition,
historical
control
data,
which
would
provide
normal
or
typical
values
for
phagocytic
activity
of
alveolar
macrophages
from
Fischer
344
rats
(determined
using
the
same
assay
method),
is
not
available.
The
toxicological
significance
of
the
changes
observed
in
this
study
is
uncertain
based
on
the
lack
of
exposure
concentration­
dependence
and
the
unavailability
of
historical
control
data.
There
were
no
test
article­
related
effects
on
respiratory
burst
activity
at
any
concentration.

­32­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
For
the
female
rats
from
the
I527
ppm
group
that
died
prior
to
the
scheduled
necropsy,
the
only
internal
macroscopic
finding
at
necropsy
was
a
dark
red
area
in
the
glandular
portion
of
the
stomach
of
one
animal.
At
the
scheduled
necropsy,
dark
red
area(
s)
on
the
liver
were
observed
for
two
males
and
a
pale
liver
was
observed
for
one
female
in
the
1527
ppm
group.
Dark
red
areas
in
the
glandular
and
non­
glandular
portions
of
the
stomach
were
observed
for
one
male
in
the
1527
ppm
group
and
dark
red
contents
in
the
cecum,
ileum,
jejunum
and/
or
stomach
were
observed
for
four
males
and
one
female
in
the
1527
ppm
group.
Dark
red
contents
in
the
urinary
bladder
were
also
observed
for
one
male
in
the
1527
ppm
group.
There
were
no
other
internal
macroscopic
findings
at
the
scheduled
necropsy.
Mean
relative
(to
final
body
weight)
kidney
weight
in
the
1527
ppm
group
males
was
significantly
(p<
O.
Ol)
higher
than
the
control
group.
The
increase
correlated
with
the
microscopic
findings
noted
for
the
kidney.
There
were
no
other
test
article­
related
effects
on
organ
weights.

Test
article­
related
microscopic
changes
were
seen
in
the
liver,
nasal
cavity,
larynx,
kidney
and
stomach.
In
the
liver,
centrilobular
hepatocellular
necrosis
(moderate
or
severe)
was
observed
in
all
animals
in
the
1527
ppm
group
and
in
4/
5
females
in
the
18
1
ppm
group.
Chlorinated,
short­
chain
hydrocarbons,
including
1
,1,2­
trichloroethane,
have
been
widely
reported
to
produce
cell
death
in
the
centrilobular
areas
of
the
liver.
The
localization
of
liver
injury
appears
to
be
related
to
the
distribution
of
microsomal
metabolizing
enzymes.
A
relatively
high
concentration
of
microsomal
metabolizing
enzymes
is
also
present
in
the
rodent
nasal
olfactory
epithelium.
In
the
current
study,
minimal
to
severe
necrosis
was
observed
in
multiple
nasal
sections
of
all
181
and
1527
ppm
group
males
and
females
with
severity
tending
to
increase
with
exposure
level
and
proximity
to
the
nares.
At
58
ppm,
all
females
and
2/
5
males
had
minimal
to
mild
olfactory
epithelial
necrosis
in
at
least
one
nasal
section.
The
nasal
olfactory
epithelium
of
the
rat
is
extremely
sensitive
to
agents
that
may
be
activated
by
cytochrome
P­
450
metabolizing
enzymes.
It
has
been
reported
that
the
fraction
of
the
nasal
airways
covered
by
olfactory
epithelium
is
approximately
50%
in
the
F344
rat
compared
to
3%
in
the
human6.
In
addition,
based
on
information
cited
by
Haschek
and
Witschi,
the
relative
surface
area
of
the
nasal
airways
(normalized
to
body
weight)
is
approximately
16
times
greater
in
the
rat
compared
to
the
human7.
As
a
result,
the
rat
may
be
considered
one
of
the
most
sensitive
animal
models
for
this
type
of
airway
epithelial
lesion.
The
finding
of
minimal
or
mild
olfactory
epithelial
cell
necrosis
for
animals
from
the
58
ppm
group
is
clearly
test
article­
related,
but
it
is
considered
unlikely
to
represent
a
toxicologically
adverse
response.
It
is
likely
that
such
minor
effects
involving
a
small
fraction
of
the
olfactory
epithelium
would
completely
resolve
following
a
single
exposure.

Single
cell
necrosis
of
the
transitional
epithelium
was
seen
in
the
turbinates
and
lateral
wall
of
nasal
sections
II
and,
variably,
III
from
all
males
and
females
in
the
1527
ppm
group.
Acute
inflammation
of
the
larynx
was
observed
in
all
females
and
3/
5
males
in
the
­33­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
1527
ppm
group.
Four
of
five
males
in
the
58
ppm
group
and
2/
5
males
in
the
181
ppm
group
also
had
minimal
laryngeal
inflammation.
It
was
unclear
why
the
highest
incidence
of
this
change
was
observed
in
the
lowest
concentration
group.
The
ventral
pouch
of
the
larynx
was
the
most
commonly
affected
area.
The
transitional
epithelium
of
the
larynx
in
the
rat
has
also
been
found
to
be
extremely
sensitive
to
injury
by
a
broad
range
of
chemical
agents6.
The
finding
of
minimal
laryngeal
inflammation
with
no
concentration­
dependence
suggests
that
this
finding
has
little
or
no
toxicological
significance.

A
small
number
of
renal
cortical
tubules
in
the
1527
ppm
group
contained
a
brightly
eosinophilic
to
basophilic
coarsely
granular
material.
The
location
of
this
luminal
debris
was
nonspecific
and
the
composition
and
significance
of
this
change
was
unclear.
In
the
glandular
stomach,
ulceration
and/
or
erosion
was
observed
at
the
1527
ppm
exposure
level.
This
change
was
not
seen
in
any
other
groups
and
was
the
most
likely
source
of
the
dark
red
areas
and
contents
seen
in
the
stomach
at
necropsy.
This
change
may
be
secondary
to
stress
and
not
a
direct
effect
of
1,1,2­
TCE
exposure.
No
other
test
article­
related
microscopic
changes
were
observed.

Based
on
the
microscopic
findings
(olfactory
epithelial
necrosis
and
acute
laryngeal
inflammation)
and
effects
on
phagocytic
activity
of
alveolar
macrophages
from
bronchoalveolar
lavage
fluid,
the
no­
observed­
effect
level
(NOEL)
for
males
and
females
was
less
than
58
ppm
when
exposed
to
l,
l,
Ztrichloroethane
as
a
single,
four­
hour
vapor
exposure
in
albino
rats.
However,
the
findings
noted
for
the
58
ppm
group
animals
were
of
questionable
toxicological
significance,
in
particular
in
relation
to
human
risk.
There
was
no
exposure
concentration­
dependence
for
the
apparent
decrease
in
alveolar
macrophage
phagocytic
activity.
The
respiratory
tract
tissues
affected
in
this
group
are
known
to
be
extremely
sensitive
to
chemical
injury
in
the
rat.
Since
olfactory
epithelium
covers
approximately
50%
of
the
rat's
nasal
airways,
minimal
to
mild
lesions
are
considered
unlikely
to
have
an
adverse
effect
on
function.
Laryngeal
inflammation
of
minimal
severity
is
also
unlikely
to
be
toxicologically
important.
Such
minor
effects
in
tissues
that
are
extremely
sensitive
in
the
rat
compared
to
other
species
may
be
misleading
in
estimating
human
risk.

­34­
mom
SUBMISSION
Kcv.
Car01
A
Kopp,
B.
S.
Group
Supervisor,
Gross
Pathology
and
DcvclopmenmlTticoiogy
xaboratory
Karen
S.
Rqan,
D.
V.
M..
D&
C.
V.
P.,
D.
A.
B.
T.
Director,
Parhology
and
Veterbny
Medicine
hhager
II,
Isolation
Toxi~
obgy
Gad
Mark
D.
V.
M..
D.
AC.
V.
P.

f
StafFPathol&
ist
Gmp
Ibbqt%
r,
Study
&.
hlysls
anti
Reports
StadyDimor
w
Dare
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
OUALITY
ASSURANCE
UNIT
STATEMENT
Date(
s)
Findings
Date(
s)
Findings
Date(
s)
of
Phase
Reported
to
Reported
to
Insuection(
s)
ected
Studv
Director
Jlanazemeq
2l27fOl
Test
Article
DispenselExposure
2/
27/
01
3/
30/
01
10/
25,26/
01
Study
Records
(Ex­
I)
10/
29/
01
10/
29/
01
10/
26/
01
Study
Records
(N­
l)
10126/
01
10/
29/
01
10/
26/
01
Study
Records
(C­
12)
10/
26/
01
10/
29/
01
10/
26/
01
Study
Records
(H­
l)
10/
26/
01
10/
29/
01
10/
26/
01
Study
Records
(P­
l)
10/
26/
01
10/
29/
01
10/
29/
01
Study
Records
(A­
1)
10/
29/
01
10/
29/
01
10126lOl
Draft
Report
(BALF
Cytology
Report)
10/
26/
01
10/
29/
O
1
10/
26,27/
01
Draft
Report
(w/
o
BALF
Cytolow
Report)
10/
29/
01
10/
29/
01
This
study
was
inspected
in
accordance
with
the
Good
Laboratory
Practice
Regulations,
the
standard
operating
procedures
of
WIL
Research
Laboratories,
Inc.,
and
the
sponsor's
protocol
and
protocol
amendment(
s)
with
the
following
exceptions.
Data
presented
in
Appendix
B
were
the
responsibility
of
BioReliance
Corporation
and
the
data
presented
in
Appendix
F
were
the
responsibility
of
Burleson
Research
Technologies.
Quality
Assurance
findings,
derived
from
the
inspection(
s)
during
the
conduct
of
the
study
and
from
the
inspections
of
the
raw
data
and
draft
report,
are
documented
and
have
been
reported
to
the
study
director.
A
status
report
is
submitted
to
management
monthly.

This
report
accurately
reflects
the
data
generated
during
the
study.
The
methods
and
procedures
used
in
the
study
were
those
specified
in
the
protocol,
its
amendments
and
the
standard
operating
procedures
of
WIL
Research
Laboratories,
Inc.

The
raw
data,
the
retention
sample(
s),
if
applicable,
and
the
final
report
will
be
stored
in
the
Archives
at
WIL
Research
Laboratories,
Inc.,
or
another
location
specified
by
the
sponsor.

Report
Audited
By:

A
Margaret
A.
Franklin
Date
Unit
Supervisor,
Quality
Assurance
Report
Reviewed
By:

f­
j&
d
7
q(­~
yfpd
d­
A
1.
Heather
L
Skilton,
B.
S.
Group
Supervisor,
Quality
Assurance
Date
­36­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
REFERENCES
1.

2.

3.

4.

5.

6.

7.
Morgan,
Kevin,
T.
(1991)
Approaches
to
the
Identification
and
Recording
of
Nasal
Lesions
in
Toxicology
Studies.
Toxicologic
Pathology,
Vol.
19,
No.
4
(Part
1):
337­
35
1.

Burleson,
G.
R.,
Fuller,
L.
B.,
Menache,
M.
G.
and
Graham,
J.
A.
(1987)
Poly
(I):
Poly
(C)­
enhanced
Alveolar
and
Peritoneal
Macrophage
Phagocytosis:
Quantification
by
a
New
Method
Utilizing
Fluorescent
Beads.
Proceedings
of
the
Society
of
Experimental
Biology
and
Medicine,
Vol.
184:
468476.

Rodgers,
K.,
eds.
(1995)
Measurement
of
the
Respiratory
Burst
of
Leukocytes
for
Immunotoxicologic
Analysis.
In:
Methods
in
Immunotoxicology,
eds.,
Vol.
2:
67­
77.

Snedecor,
G.
W.
and
Co&
ran,
W.
G.
(1980)
One­
way
classification;
analysis
of
variance.
In:
Statistical
Methods,
Seventh
Edition.
The
Iowa
State
University
Press,
Ames,
IA,
pp.
215­
237.

Dunnett,
C.
W.
(1964)
New
tables
for
multiple
comparisons
with
a
control.
Biometrics,
20:
482­
491.

Dungworth,
D.
L.,
Hahn,
F.
F.
and
Nikula,
K.
J.
(1995)
Noncarcinogenic
Responses
of
the
Respiratory
Tract
to
Inhaled
Toxicants.
In:
Concepts
in
Inhalation
Toxicology,
2"
d
Edition,
McClellan,
R.
O.
and
Henderson,
R.
F.,
eds.,
Taylor
&
Francis,
Washington,
DC.,
pp.
533­
576.

Haschek,
W.
M.
and
Witschi,
H.
R.
(1991)
Respiratory
System.
In:
Handbook
of
Toxicologic
Pathology,
Haschek,
W.
M.
and
Rousseaux,
C.
G.,
Academic
Press,
Inc.,
pp.
761­
827.

­37­
w&
417001
Hazardous
Air
Pollutant
Task
Force
DEVIATIONS
FROM
THE
PROTOCOL
This
study
was
conducted
in
accordance
with
the
protocol
and
protocol
amendment(
s),
if
applicable,
except
for
the
following:

1.
ExDerimental
Design.
For
the
animal
exposure
at
181
ppm
on
February
25,2001,
the
exposure
period
was
extended
54
minutes
to
compensate
for
a
syringe
pump
failure
that
resulted
in
a
period
of
low
atmosphere
concentration.

2.
Test
Atmosphere
Stability
and
Homoe;
eneitv.
Spatial
homogeneity
was
performed
for
Chambers
2
and
4
after
animal
exposures
on
March
2
and
5,
2001,
instead
of
during
pre­
study
method
development.
Homogeneity
was
not
conducted
on
Chamber
3.

3.
Chemical
Puritv
of
the
Test
Atmosphere.
Chemical
purity
of
the
high
concentration
atmosphere
was
conducted
during
animal
exposures
on
February
26
and
27,
2001,
instead
of
during
pre­
study
method
development.
In
addition,
chemical
purity
was
not
performed
in
triplicate
as
required.

4.
Absence
of
Aerosol
in
the
Test
Atmosphere.
Verification
of
the
absence
of
aerosol
was
performed
during
animal
exposure
on
February
26,
2001,
instead
of
during
pre­
study
method
development.

5.
Bronchoalveolar
Lavage
Evaluation.
Lavages
were
performed
with
Hank's
Balanced
Salt
Solution
instead
of
physiological
saline.

6.
Bronchoalveolar
Lavane
Evaluation.
Individual
lavage
volumes
were
4­
5
ml
instead
of
the
specified
5­
6
ml.

7.
Bronchoalveolar
Lavage
Evaluation.
The
study
protocol
stated
that
information
regarding
methods,
quality
assurance
and
archiving
activities
and
reporting
for
the
subcontracted
alveolar
macrophage
functional
assays
would
be
described
in
a
protocol
amendment.
Instead,
this
information
was
included
in
the
report
and
study
records
produced
by
Burleson
Research
Technologies,
Inc.

These
deviations
did
not
negatively
impact
the
quality
or
integrity
of
the
data
nor
the
outcome
of
the
study.

Study
Director
­38­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
Figure
1:
Atmosphere
Generation
and
Exposure
System
(Low
and
Mid
Concentration
Exposure
Groups)

Heated
'
Vaporization
2
Column
c
500­
Liter
Chamber
­39­
WlL­
417001
Hazardous
Air
Pollutant
Task
Force
Figure
2:
Atmosphere
Generation
and
Exposure
System
(High
Concentration
Exposure
Group)

500­
Liter
Chamber
Test
Article
I
L
T
Reservoir
­4o­
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,1,2­
Trichloroethane
(1~
1,2­
TCE)
in
Rats
Tables
l­
23
­41­
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
1
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
INDIVIDUAL
TEST
ATMOSPHERE
CONCENTRATIONS
PAGE
1
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

CONCBNTRATIONS
FOR
STUDY
DAY:
0
EXPOSURE
DATE:
25­
FEB­
01
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GROUP:
0
PPM
58
PPM
181
PPM
1527
PPM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

TIME
INTO
CONC
.
TIME
INTO
CONC
.
TIME
INTO
CONC
.
TIME
INTO
CONC
.
EXPOSURE
fHH:
MM)
(PPM)
EXPOSURE
(HH:
MM)
IPPMM)
EXPOSURE
(HH:
MM)
fPPM)
EXPOSURE
(HH:
MM)
(PPM)
___________________________________
I____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
00:
38
0.
01:
oz
0.
01:
47
0.
02:
35
0.
03:
16
0.
03:
48
0.

____­_______________­­­­­­­­­­
MEAN
0.
SD
0.0
WI%)
0.00
N
6
00:
29
54.
00:
57
19.
01:
12
56.
01:
42
78.
02:
os
74.
02:
31
66.
03:
ll
64.
03:
34
49.
00:
34
212.
00:
23
1506.
00:
53
209.
00:
47
1485.
01:
37
0.
01:
32
1506.
01:
50
27.
02:
22
1538.
02:
lO
286.
03:
Ol
1563.
02:
26
221.
03:
40
1564.
03:
06
208.
03:
44
219.
04:
13
221.
04:
38
206.
.­

.­
__________­_________­____________­­___­_________­___­­­­­­_______________­­­_____________________­_
58.
181.
1527.
18.4
91.4
33.0
32.04
50.55
2.16
8
10
6
.­

.­

_­
43
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
1
ACUTE
INH.
TOX
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
TEST
ATMOSPHERE
CONCENTRATIONS
PAGE
3
_____________"_"__­­"""""­­""~""~­"­­""""~~~"~~"~~~~"~~~~~~~­""~"~~~~~~­"~~"~~~­­~"~~"~~"~~~­~"""~~"~~~­~""­~~~"""~­~""~~~""~~~"~"~

CONCENTRATIONS
FOR
STUDY
DAY:
0
EXPOSURE
DATE:
27­
FEE­
01
_____________­______~"~"""""~""""­"~~~~~"~~""~~~""~~""""""~~"~~~""~"~"~~~­""""~~~"~~­~""~~""~~~~"""­~~"""~"­"~"­~""~~~­~"
"____""""_

GROUP:
0
PPM
60/
45
PPM
205/
117
PPM
1474/
840
PPM
_____________"____­__________________
I__­­­"""­­­­"­­­­­­"­­""­­""­­­"­­""­"­"­­­­"­­­"­­­­""""­"­­­­­­­­""­"""­­­­­­­­­­­""­­"""­­
TIME
INTO
EXPOSURE
(HH:
MM)
_____­­__­­­­­­­­­­­­
00:
35
01:
19
01:
39
02:
Ol
02:
45
03:
14
03:
45
CONC
.
(PPM)

0.
0.
0.
0.
0.
0.
0.
TIME
INTO
EXPOSURE
(HH:
MM)
____­­__­____­­­___

00:
29
01:
15
01:
34
01:
sa
02:
42
03:
lO
03:
41
(PPM)
"
­
­
_
"
­
­
­
"
­

48.
36.
40.
47.
55.
43.
49.
TIME
INTO
CONC
.
TIME
INTO
EXPOSURE
(HH:
MM)
(PPM)
EXPOSURE
(HH:
MM)

00:
46
94.
01:
lO
124.
01:
30
164.
01:
55
187.
02:
lO
228.
02:
37
226.
03:
07
180.
03:
3a
212.
00:
22
995.
01:
05
664.
01:
27
585.
01:
51
613.
02:
05
761.
02:
33
1071.
03:
04
914.
03:
34
965.
04:
16
989.

________________________________________"~~~"~"~~~~"~~~~~"~~"~"~~~~"~~­"~~~"~"~­~­"~""~~~"~~~~~~~~"""­~­"

MEAN
0.
45.
177.
SD
0.0
6.3
48.1
CV(%)
0.00
13.86
27.20
N
7
7
a
(PPM)

840.
185.2
22.06
9
PCHCv4.0
06/
12/
2001
R:
10/
29/
2001
.­

.­.
TABLE
2
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOSITIONS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
1
SPONSOR:
HAP
TASK
FORCE
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
­____
MALE
­­­­­
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
GROUP:
1
2
3
4
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­

NORMAL
­NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
­SCHEDULED
EUTHANASIA
BEHAVIOR/
CNS
­LETHARGIC
CARDIO­
PULMONARY
­RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
­DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
­DRIED
RED
MATERIAL
AROUND
NOSE
EXCRETA
­URINE
APPEARS
REDDISH
BROWN
IN
COLOR
ORAL/
DENTAL
­DRIED
RED
MATERI?&
AROUND
MOUTH
_____________
s______­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­
­______

l­
0
PPM
2­
58
PPM
3­
181
PPM
101
5
5/
5
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
,__­___
4­
10/
5
51
5
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
__­­______­____­­__­­­­­­­­­­­­
1527
PPM
101
5
5/
5
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
______­___­________­___
51
5
41
4
1/
1
21
2
11
1
l/
1
­

.­

.­

.­

.­
PAGE
2
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAp
TASK
FORCE
TABLE
2
(DETAILED
PHYSICAL
BXAMINATI~
NS/
DI.
SP~
SITI~
NS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
__­­­
F
E
,i,
A
L
E
_____
__________________________
I_____________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
GROUP:
1
2
3
4
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

NORMAL
­NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
­FOUND
DEAD
­SCHEDULED
EUTHANASIA
BEHAVIOR/
CNS
­LETHARGIC
BODY/
INTEGUMENT
­BODY
COOL
TO
TOUCH
­DRIED
RED
MATERIAL
ON
DORSAL
HEAD
­DRIED
RED
MATERIAL
ON
FORELIMB(
S)

CARDIO­
PULMONARY
­RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
­CLEAR
DISCHARGE
RIGHT
EYE
­CLEAR
DISCHARGE
LEFT
EYE
­DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
_______________­____­­­­­­­­­­­­­­­­­­
l­
o
PPM
2­
58
PPM
lO/
5
01
0
5/
5
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
___­__­___­_____________
3­
181
PPM
4­
10/
5
01
0
5/
5
01
0
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
___________­­______­­­­­­­­­­­­­­­­~
1527
PPM
o/
0
s/
5
o/
0
01
0
o/
0
o/
0
Of
0
01
0
o/
0
1/
1
­_­­­­­­­­­___­­­­_____
41
4
31
3
2/
2
1/
1
1/
1
I/
1
1/
1
2/
2
2/
2
21
2
2/
2
TABLE
2
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOSITIONS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
__­__
F
E
M
A
L
E
_____
PAGE
3
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­
TABLE
WINGE:
02­
25­
01
TO
02­
26­
01
GROUP:
1
2
3
4
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

EYES/
EARS/
NOSE
­DRIED
RED
MATERIAL
AROUND
LEFT
EYE
o/
0
o/
0
o/
0
11
1
­DRIED
RED
MATERIAL
AROUND
NOSE
o/
0
o/
0
01
0
3/
3
ORAL/
DENTAL
­DRIED
RED
MATERIAL
AROUND
MOUTH
01
0
o/
0
o/
0
2/
2
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­
I.­
0
PPM
2­
5a
PFM
3­
181
PPM
4­
1527
PPM
PCSUV4.0
06/
18/
2001
(
I
!
!
I
!
i
!
(
!
I
~
I
!
!
!
!
!
/
!
!
I
!
!
'
!

48
/
!
I
/
!
!
i
I
I
/
I
t
I
!
/
!
~
!
!
I
I
!
I
49
I
I
!
,
/
1
,
,
!
I
!
!
/
/
I
I
/
/
!
~
I
I
!
II
/
~
/
I
,
I
I
m
51
TABLE
3
(IMMEDIATELY
FOLLOWING
EXPOSURE)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
1
SPONSOR:
HAP
TASK
FORCE
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
­­­__
MALE
­_­_­
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

TABLE
RANGE:
GROUP:
________________________________________­­­­­­­­­­­­­­­

NORMAL
­NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
BEHAVIOR/
CNS
­ANIMAL
SLEEPING
CARDIO­
PULMONARY
­RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
­CLEAR
DISCHARGE
RIGHT
EYE
­CLEAR
DISCHARGE
LEFT
EYE
______­­­______­__­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

l­
o
PPM
2­
58
PPM
3­
181
PPM
02­
25­
01
1
2
3
4
­_____­­­­__________
II__________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

51
5
o/
0
01
0
o/
0
o/
0
_­­­­­
4­
5/
5
5/
5
o/
0
Of
0
o/
0
5/
5
o/
0
o/
0
s/
5
o/
0
o/
0
5/
5
o/
0
o/
0
5/
5
­­­­­­___­­­­­______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­
1527
PPM
I
/
I
!
!
/
!
'
/
/
I
!
/
/

53
54
55
I
II'
I
I
/
I
!
!
!
!
!
!
!
!
!
!

56
57
i
i
!
!
III
/
!
!
!
!
/
!
!
!
/
!
!
!
!

I
,

58
TABLE
4
(RESPONSE
TO
NOVEL
STIMULUS)
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
2
SPONSOR:
HAP
TASK
FORCE
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
_­___
F
E
M
A
L
8
____­
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

TABLE
RANGE:
02­
27­
01
GROUP:
1
2
3
4
BEHAVIOR/
CNS
­NO
REACTION
TO
STIMULUS
­SLIGHT
REACTION
­NOTABLY
INCREASED
REACTION
­NOT
VISIBLE
_____________­___­__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
l­
0
PPM
2­
60/
45
PPM
3­
205/
177
PPM
­­
21
2
4/
4
4/
4
5/
5
I/
1
I/
1
l/
1
o/
0
2/
2
01
0
o/
0
o/
0
o/
0
o/
0
o/
0
o/
0
_____________­__________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
4­
1474/
840
PPM
MANUALVl
.
0
06/
20/
2001
R:
06/
20/
2001
.­

.­
60
I
I
!
I
!
I
I
!
/
II
:
/
!
!
!
1
I
,
/
!
I
/
/
,
I
I
/
/
'
!
/

61
I
,
!
I
I
!
I
I
!
I
/
/
I
!
I
!
/
!
/
/
I
I
!
!
!
!
/
!
!

62
/
1
!
/
/
!
/
!
/
I
/
!
/
!
1
1
!
!
I
I
!
/
!
!
!
!
I
/
I
!

63
64
,

.IDm
m
.
l­
4
.mul
r­
N
.u)
m
t­
4
.
ri
.Nul
I+
.
t­
4
65
I
I
I
I
I
/
/
!
I
I
!
I
/
/
/
!
!
I
!
I
~
!
!
!
!
!
!
!
!
!
1
!
!
~
!
/
I
/
!
I
/
!
!
~
!
1
!
!
!
!
!
!
!
!
/
!
I
!
,

67
I
/
I
/
!
!
!
!
I
/
I
!
!
/
/
!
i
/
/
/
!
I
!
~
I
I
!
/
I
!
/
!
!
!
!
!
!
!
/
/
/
!

69
I
I
!
I
/
!
!
!
!
I
/
I
t
I
,
I
I
!
I
!
!
!
)
/
TABLE
9
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
GROSS
NECROPSY
OBSERVATIONS
INCIDENCE
SUMMARY
PAGE
1
SCHEDULED
NECROPSY
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
_____
M
A
L
E
­­­­
­­­­
F
E
M
A
L
E
­­­­
GROUP:
12
3
4
12
3
4
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
DAY
1
5
5
5
5
5
5
CECUM
­DARK
RED
CONTENTS
0
5
5
0
0
0
0
0
0
0
0
0
0
0
5
5
5
0
0
0
0
0
0
0
0
0
0
0
5
1
0
ILEUM
­DARK
RED
CONTENTS
0
1
0
JEJUNUM
­DARK
RED
CONTENTS
0
3
0
LIVER
­DARK
RED
AREA(
S)
­PALE
0
0
2
0
0
0
SKIN
­RED
MATTING
­YELLOW
MATTING
0
0
2
0
0
0
SPLEEN
­ACCESSORY
0
0
1
STOMACH
­DARK
RED
AREA(
S)
­DARK
RED
CONTENTS
0
0
1
3
0
0
URINARY
BLADDER
­DARK
RED
CONTENTS
0
NO
SIGNIFICANT
CHANGES
OBSERVED
­
ALL
EXAMINED
TISSUES
5
1
1
0
4
5
5
0
0
0
0
0
0
0
0
0
0
0
5
5
5
0
0
0
0
0
1
0
0
0
0
0
4
5
2
0
0
0
0
1
2
1
0
0
1
0
0
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

l­
0
PPM
2­
58
PPM
3­
181
PPM
4­
1527
PPM
PGRSIv4.02
06/
12/
2001
I
/
!
!
I
!
!
!
!
!
/
I
I
/
I
I
I
/
I
/
!
I
I
!
/
/

73
,
,
,

,
,
I
,

I
I
4
I
I
I
1
I
4
I
I
1
1
1
,
,
I
,
I
I
I
,
I
I
75
!
/
!
!
!
!
!
I
!
!
/
)
/
!
!
!
/
I
I
1
1
!
!
/
1
1
1
I
76
!
I
!
!
!
!
!
!
!
I
!
!
I
/
I
/
I
I
!
/
I
I
!
I
!
/
/
!
/
!
!
!
!
!
!
!
!
!
!
/
/
/
,,
I
/
/
!
I
i
!
~
/
/
!

78
79
I
/
II
/
1
/
!
I
I
!
!
!
!
!
I
I
/
/
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!
!
!
/
I
/
I
/
/

m
u­
lo
Loo
mo
00
522
ti2B
00
00
P$

PC2
00
00
BB
PP
00
00
BB
852
OO
00
Pb
Li2B
00
00
ciE2
Bb
00
80
81
,
I
,
I
t
,

82
TABLE
12
(UNSCHEDULED
DEATHS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
4
SPONSOR:
HAP
TASK
FORCE
HISTOMORPHOLOGICAL
DIAGNOSIS
­­
SUMMARY
INCIDENCE
__­__
FE­
E:
­­­__
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
1
2
3
4
­­­.
________________­___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GROUP:
_________________­______________________­­­­­­­­­­­­­­­­­­­
NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
5
0
5
0
5
5
0
3
ADRENAL
CORTEX
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
ADRENAL
MEDULLA
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
KIDNEYS
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
TUBULAR
MINIMAL
­LUMINAL
DEBRIS
MINIMAL
LIVER
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
SEVERE
0
NA
NA
3
0
NA
NA
3
0
NA
NA
3
0
NA
NA
3
0
NA
NA
3
0
NA
NA
1
0
NA
NA
1
NONE
NA
NA
1
0
NA
NA
2
NONE
NA
NA
2
0
0
0
NONE
NA
NA
3
NA
NA
0
NA
NA
3
NA
NA
3
__­­_
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
l­
0
PPM
2­
5a
PPM
3­
la1
PPM
4­
1527
PPM
NA
=
NOT
APPLICABLE
84
I
!
I
I
!
!
I
I
/
I
!
!
!
I
I
!
!
!
!
!
!
~
/

;
,
I
m
85
TABLE
12
(UNSCHEDULED
DEATHS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
7
SPONSOR:
HAP
TASK
FORCE
HISTOMORPHOLOGICAL
DIAGNOSIS
­­
SUMMARY
INCIDENCE
___­­
FFJq&
LE
­­­­­
____________________­­­­­­­­­­­­­­­­­­­­­­
______­_____­___­­______________
i_______­­­­­­­­­­­­­.­­­.­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GROUP:
1
2
3
4
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
­­­­­­
I
NUMBER
OF
ANIMALS
IN
DOSE
GROUP
5
5
5
5
NUMBER
OF
ANIMALS
EXAMINED
0
0
0
3
NASAL
LEVEL
IV
TOTAL
NUMBER
EXAMINED
0
NA
NA
3
EXAMINED,
UNREMARKABLE
0
NA
NA
0
­NECROSIS,
OLFACTORY
EPITHELIUM
0
NA
NA
3
MILD
NONE
NA
NA
1
MODERATE
NONE
NA
NA
1
SEVERE
NONE
NA
NA
1
NASAL
LEVEL
V
TOTAL
NUMBER
EXAMINED
0
NA
NA
3
EXAMINED,
UNREMARKABLE
0
NA
NA
0
­NECROSIS,
OLFACTORY
EPITHELIUM
0
NA
NA
3
MINIMAL
NONE
NA
NA
1
MILD
NONE
NA
NA
2
NASAL
LEVEL
VI
TOTAL
NUMBER
EXAMINED
0
NA
NA
3
EXAMINED,
UNREMARKABLE
0
NA
NA
0
­NECROSIS,
OLFACTORY
EPITHELIUM
0
NA
NA
3
MINIMAL
NONE
NA
NA
1
MILD
NONE
NA
NA
2
______________________________________________________________________­____________­____­­­­­­­__­~­­­­~­~~~­~__­______~__________
l­
0
PPM
2­
58
PPM
3­
181
PPM
4­
1527
PPM
NA
=
NOT
APPLICABLE
87
88
I
,

1
I
,
,
1
1
1
1
I
I
/
,

1
I
/
I
,
!
I
!
!
!
~
I
I
I
/
!
/
/
I
!
!
!
/
!
!
/
/
:
:

w
4
g;
,
,
,
,
I
,
I
(
,
'
I
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
13
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOK.
(WITH
HISTOPATHOLOGY)
STUDY
0~
1.1,2­
TCE
IN
RATS
PAGE
5
HISTOMORPHOLOGICAL
DIAGNOSIS
­­
SUMMARY
INCIDENCE
­­­­­
­E
_­_­_

________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GROUP:
1
,_______­­­­_­__­­­­­______­­_­­­­­­­­­­­­­

5
5
2
________­_________­_­­­­­­­­­­­­­­­­­­­­­­­­­
NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
DAY
1
URINARY
BLADDER
5
5
3
.­­­­­­­­­­­­­­
5
5
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
NA
NA
NA
NA
NA
NA
NASAL
LEVEL
I
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
5
NA
NA
5
NA
NA
NASAL
LEVEL
II
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
SINGLE
CELL
MINIMAL
MILD
­INFLAMMATION,
SUBACUTE
MINIMAL
­NECROSIS,
RESPIRATORY
EPITHELIUM
MINIMAL
­METAPLASIA,
SQUAMOUS
MINIMAL
5
2
0
NONE
NONE
2
3
2
3
0
0
NONE
NONE
0
1
NONE
1
5
5
0
NONE
NONE
0
NONE
0
NONE
0
NONE
4
.­­­­­
5
5
1
1
5
5
5
0
5
2
3
0
NASAL
LEVEL
III
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
SINGLE
CELL
MINIMAL
MILD
5
5
5
5
5
5
1
0
0
0
0
3
NONE
NONE
NONE
1
NONE
NONE
NONE
2
NONE
1
1
0
NONE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
l­
0
PPM
2­
58
PPM
3­
181
PPM
4­
1527
PPM
NA
=
NOT
APPLICABLE
I
!
/
1
!
!
!
!
I
!
!
!
!
!
/
~
!
!
I
!
/
I
1
!
!
!
I
1
!
!
I
f
/
1
/
/
!
/
!
/
!
/
!
I
!
!
~~
/
!
I
/
/
/
/
/
/
/
'
/
1
/
!
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
13
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
HISTOMORPHOLCGICAL
DIAGNOSIS
­­
SUMMARY
INCIDENCE
PAGE
8
­­­­­
FEMALE
__­­­

_________________­_
C__________
L_________"­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

________­_______­__­­­­­­­­­­­­­

NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
DAY
ADRENAL
CORTEX
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS
MINIMAL
ADRENAL
MEDULLA
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
DUODENUM
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­HYPERPLASIA,
EPITHELIAL
MILD
­INFLAMMATION,
ACUTE
MINIMAL
KIDNEYS
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NEPHROPATHY
MINIMAL
­INFLAMMATION,
CHRONIC
MINIMAL
GROUP:
1
_____________­­­­­­_­­­­­­~­­­­­­­­­­

5
5
5
NA
NA
2
5
NA
NA
1
0
NA
NA
1
NONE
NA
NA
1
5
NA
NA
2
5
NA
NA
2
NA
NA
NA
1
NA
NA
NA
0
NA
NA
NA
1
NA
NA
NA
1
NA
NA
NA
1
NA
NA
NA
1
2
3
4
.__­­
L­­____­­______­­­­­­­­­­­­­­­­­­­­­­­­
5
5
5
5
5
2
5
5
2
5
5
1
0
0
0
NONE
NONE
NONE
0
0
0
NONE
NONE
NONE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
l­
o
PPM
2­
58
PPM
3­
181
PPM
4­
1527
PPM
NA
=
NOT
APPLICABLE
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
13
(SCHEDULED
NECROPSYI
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
HISTOMORPHOLOGICAL
DIAGNOSIS
­­
SUMMARY
INCIDENCE
__­­­
FEMALE
­
­
­
­
­
PAGE
9
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GROUP:
1
2
3
4
_______­________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
DAY
1
KIDNEYS
­
CONTINUED
­LUMINAL
DEBRIS
MINIMAL
LIVER
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
HEPATOCELLULAR,
CENTRILGBULkR
MINIMAL
MILD
MODERATE
­INFILTRATE,
MONONUCLEAR
MINIMAL
­INFLAMMATION,
SUBACUTE
MINIMAL
MILD
LARYNX
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­EDEMA
MINIMAL
MILD
­INFLAMMATION,
ACUTE
MINIMAL
MILD
___________­________­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­
l­
0
PPM
2­
58
PPM
3­
181
PPM
5
5
5
1
0
NONE
NONE
NONE
4
4
0
NONE
NONE
5
3
1
1
NONE
2
2
NONE
4­
1527
PPM
5
5
0
0
NONE
NONE
il
NONE
NONE
NONE
4
4
0
NONE
NONE
5
4
0
NONE
NONE
1
I
NONE
__
c­­­­­­___________
5
5
5
2
0
0
4
2
3
NONE
1
NONE
NONE
2
1
0
1
NONE
3
0
1
NONE
2
NONE
5
2
4
0
0
1
NONE
NONE
NONE
1
1
2
1
NONE
NONE
2
5
2
1
1
I
I
/
!
/
!
!
!
I
!
!
I
!
!
!
!
!
'
/
!
!
!
!
!
!
I
/
/
I
I
I
PAGE
11
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
13
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
0~
l,
l,
z­~
C~
IN
RATS
HISTOMORPHOLOGICIU
DIAGNOSIS
­­
SUMMARY
INCIDENCE
_____
F&­,&
Z&
E
__­__
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GROUP:
1
2
3
4
_______________­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

NUMBER
OF
ANIMALS
IN
DOSE
GROUP
NUMBER
OF
ANIMALS
EXAMINED
DAY
1
5
5
5
5
5
5
5
2
TRACHEA
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
NASAL
LEVEL
I
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
NASAL
LEVEL
II
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
SINGLE
CELL
MINIMAL
MILD
­INFLAMMATION,
SUBACUTE
MINIMAL
­METAPLASIA,
SQUAMOUS
MINIMAL
­EXUDATE
MINIMAL
NASAL
LEVEL
III
TOTAL
NUMBER
EXAMINED
EXAMINED,
UNREMARKABLE
­NECROSIS,
SINGLE
CELL
MINIMAL
MODERATE
____________________­­­­­­­­­­­­­­­­­­­­­­­­

l­
0
PPM
2­
58
PPM
3­
NA
=
NOT
APPLICABLE
5
NA
NA
2
5
NA
NA
2
5
NA
NA
2
5
NA
NA
2
5
5
5
2
3
2
3
0
0
0
1
2
NONE
NONE
1
1
NONE
NONE
NONE
1
2
1
0
0
2
1
NONE
NONE
1
2
1
0
1
2
1
NONE
0
0
1
0
NONE
NONE
1
NONE
5
5
5
2
4
3
1
0
0
0
0
2
NONE
NONE
NONE
1
NONE
NONE
NONE
1
________­____­­_____­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
181
PPM
4­
1527
PPM
/
i
/
/
:
I
I
i
I
!
I
I
/
1
!
!
I
!
!
!
!
;
m
TABLE
14
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOSITIONS)
PROJECT'NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
1
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
______________________________________^_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­*­­­­­­­­­

61409
M
0
PPM
NORMAL
02­
25­
01
7:
49
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
24
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61409
M
0
PPM
DISPOSITION
02­
26­
01
13:
05
P
SCHEDULED
EUTHANASIA
61410
M
0
PPM
NORMAL
02­
25­
01
7:
49
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
24
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61410
M
0
PPM
DISPOSITION
02­
26­
01
13:
05
P
SCHEDULED
EUTHANASIA
61419
M
0
PPM
NORMAL
02­
25­
01
7:
50
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7~
24
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61419
M
0
PPM
DISPOSITION
02­
26­
01
13:
06
P
SCHEDULED
EUTHANASIA
61432
M
0
PPM
NORMAL
02­
25­
01
7:
SO
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
25
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61432
M
0
PPM
DISPOSITION
02­
26­
01
13:
06
P
SCHEDULED
EUTHANASIA
61434
M
0
PPM
NORMAL
02­
25­
01
7:
50
P
NO
SIGNIFICANT
CLINICAL
QBSERVATIONS
02­
26­
01
7:
25
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61434
M
0
PPM
DISPOSITION
02­
26­
01
13:
06
P
SCHEDULED
EUTHANASIA
61396
M
58
PPM
NORMAL
02­
25­
01
7:
51
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7~
25
F
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61396
M
58
PPM
DISPOSITION
02­
26­
01
13:
06
P
SCHEDULED
EUTHANASIA
61405
M
58
PPM
NORMAL
02­
25­
01
7:
51
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
26
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61405
M
58
PPM
DISPOSITION
02­
26­
01
13:
06
P
SCHEDULED
EUTHANASIA
61413
M
58
PPM
NORMAL
02­
25­
01
7:
51
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61413
M
58
PPM
DISPOSITION
02­
26­
01
13:
0­
l
P
SCHEDULED
EUTHANASIA
61416
M
58
PPM
NORMAL
02­
25­
01
7~
51
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7~
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61416
M
58
PPM
DISPOSITION
02­
26­
01
13:
07
P
SCHEDULED
EUTHANASIA
61431
M
58
PPM
NORMAL
02­
25­
01
7:
52
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
__________________
I________­­___­______________­___­___________________.___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
RAP
TASK
FORCE
TABLE
14
(DETAILED
PHYSICAL
EXAMINATI~
NS/
DISP~
SITIONS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
PAGE
2
__________­___­_________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­
ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61431
M
58
PPM
61399
M
181
PPM
61399
M
61411
M
181
PPM
181
PPM
61411
M
61441
M
181
PPM
181
PPM
61441
M
61442
M
181
PPM
181
PPM
61442
M
61443
M
191
PPM
181
PPM
61443
M
61397
M
61397
M
61397
M
61397
M
61397
M
61406
M
61406
M
61406
M
61408
M
61408
M
61408
M
61408
M
61421
M
191
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
1527
PPM
DISPOSITION
02­
26­
01
13:
07
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
52
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
07
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
52
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
31
P
No
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
07
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
53
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
32
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
08
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
53
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7~
32
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
Os
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
53
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
33
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
08
P
SCHEDULED
EUTHANASIA
NORMAL
02­
25­
01
7:
54
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
08
P
SCHEDULED
EUTHANASIA
BEHAVIOR/
CNS
02­
26­
01
7:
34
P
LETHARGIC
CARDIO­
PULMONARY
02­
26­
01
7:
35
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
26­
01
7:
34
P
DRIED
RED
MATERIAL
AROUND
NOSE
NORMAL
02­
25­
01
7:
54
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
OB
P
SCHEDULED
EUTHANASIA
BEHAVIOR/
CNS
02­
26­
01
7~
36
P
LETHARGIC
NORMAL
02­
25­
01
7:
54
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
26­
01
13:
08
P
SCHEDULED
EUTHANASIA
BEHAVIOR/
CNS
02­
26­
01
7:
38
P
LETHARGIC
CARDIO­
PULMONARY
02­
26­
01
7~
38
P
RESPIRATION
RATE
DECREASED
NORMAL
02­
25­
01
7:
54
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
______________­_____­­­­"­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­"­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­"­­­­­­"­"­­­­­­­­­­­­­­­­­­­

GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
.__­
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ANIMAL
SEX
­­­­_­­­­­­_­

61421
M
61421
M
1527
PPM
1527
PPM
61421
M
1527
PPM
61439
M
1527
PPM
61439
M
1527
PPM
61439
M
1527
PPM
61439
M
1527
PPM
61439
M
1527
PPM
61446
F
0
PPM
61446
F
61452
F
0
PPM
0
PPM
61452
F
61453
F
0
PPM
0
PPM
61453
F
61456
F
0
PPM
0
PPM
61456
F
61491
F
0
PPM
d
PPM
61491
F
61467
F
0
PPM
58
PPM
61467
F
61470
F
58
PPM
58
PPM
DISPOSITION
02­
26­
03
13:
08
EYES/
BARS/
NOSE
02­
26­
01
7:
38
02­
26­
01
7:
39
ORAL/
DENTAL
02­
26­
01
7:
38
NORMAL
02­
25­
01
7:
55
DISPOSITION
02­
26­
01
13:
08
BEHAVIOR/
CNS
02­
26­
01
7:
40
CARDIO­
PULMONARY
02­
26­
01
7:
40
EXCRETA
02­
26­
01
7:
44
NORMAL
02­
25­
01
7:
56
02­
26­
01
7:
44
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
57
02­
26­
01
7:
45
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
57
02­
26­
01
7:
45
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
57
02­
26­
01
7:
45
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
sa
02­
26­
61
7:
46
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
sa
02­
26­
01
7:
46
DISPOSITION
02­
26­
01
13:
09
NORMAL
02­
25­
01
7:
58
02­
26­
01
7:
46
_________­__________­­­­­­­­­­­­­­­
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
P
­_­
SCHEDULED
EUTHANASIA
DRIED
RED
MATERIAL
AROUND
NOSE
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
DRIED
RED
MATERIAL
AROUND
MOUTH
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
LETHARGIC
RESPIRATION
RATE
DECREASED
URINE
APPEARS
REDDISH
BROWN
IN
COLOR
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
SCHEDULED
EUTHANASIA
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
___""­­­­__"­­­"___­"­­­"­­­­­­"­"­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­""
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
14
(DETAILED
PHYSICAL
RxAMINATIONS/
DISP~
SITI~
NS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1.2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
PAGE
3
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
14
(DETAILED
PHYSICAL
ExAMINATION~/
DI~
P~
SITI~
NS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
PAGE
4
ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
______­­______­­­­­­­­­­
_____­­_____­­­
____________­___­___________________
I___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61470
F
58
PPM
DISPOSITION
02­
26­
01
13:
09
P
SCHEDULED
EUTHANASIA
61472
F
58
PPM
NORMAL
02­
25­
01
7:
59
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
61
7:
47
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61472
F
58
PPM
DISPOSITION
02­
26­
01
13:
09
P
SCHEDULED
EUTHANASIA
61475
F
58
PPM
NORMAL
02­
25­
01
7:
59
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
47
P
NO
SIGNIFICANT
CLIN1CAL
OBSERVATIONS
61475
F
58
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61477
F
50
PPM
NORMAL
02­
25­
01
7:
59
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
48
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61477
F
56
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61449
F
181
PPM
NORMAL
02­
25­
01
7:
59
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
48
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61449
F
181
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61474
F
181
PPM
NORMAL
02­
25­
01
8:
OO
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
03
7:
49
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61474
F
181
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61482
F
181
PPM
NORMAL
02­
25­
01
8:
00
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
49
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61482
F
181
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61486
F
I81
PPM
NORMAL
02­
25­
01
8:
OO
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61486
F
181
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61486
F
181
PPM
EYES/
EARS/
NOSE
02­
26­
01
7:
49
P
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
61493
F
181
PPM
NORMAL
02­
25­
01
8:
OO
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
7:
50
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61493
F
l81
PPM
DISPOSITION
02­
26­
01
13:
lO
P
SCHEDULED
EUTHANASIA
61451
F
1527
PPM
DISPOSITION
02­
26­
01
8:
03
P
FOUND
DEAD
61462
F
1527
PPM
NORMAL
02­
25­
01
8:
Ol
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61462
F
1527
PPM
DISPOSITION
02­
26­
01
12:
44
P
FOUND
DEAD
61462
F
1527
PPM
BEHAVIOR/
CNS
02­
26­
01
7:
53
P
LETHARGIC
_________­______________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­

GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
TABLE
14
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOSITIONS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGYI
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
5
SPGNSOR:
HAP
TASK
FORCE
INDIVIDUAL
CLINLCAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
26­
01
_____________­__________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61462
F
1527
PPM
BODY/
INTEGUMENT
02­
26­
01
7:
53
P
BODY
COOL
TO
TOUCH
61462
F
1527
PPM
CARDIO­
PULMONARY
02­
26­
01
7:
53
P
RESPIRATION
RATE
DECREASED
61462
F
1527
PPM
EYES/
EARS/
NOSE
02­
26­
01
7:
52
P
DRIED
RED
MATERIAL
AROUND
NOSE
02­
26­
01
7:
52
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
26­
01
7:
52
P
CLEAR
DISCHARGE
LEFT
EYE
02­
26­
01
7:
52
P
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
61476
F
1527
PPM
NORMAL
02­
25­
01
8:
Ol
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61476
F
1527
PPM
DISPOSITION
02­
26­
01
13:
ll
P
SCHEDULED
EUTHANASIA
61476
F
1527
PPM
BODY/
INTEGUMENT
02­
26­
01
7:
55
P
DRIED
RED
MATERIAL
ON
DORSAL
HEAD
02­
26­
01
7:
56
P
DRIED
RED
MATERIAL
ON
FORELIMB(
S)
61476
F
1527
PPM
CARDIO­
PULMONARY
02­
26­
01
7~
56
P
RESPIRATION
RATE
DECREASED
61476
F
1527
PPM
EYES/
EARS/
NOSE
02­
26­
01
7:
54
P
DRIED
RED
MATERIAL
AROUND
NOSE
02­
26­
01
7:
54
P
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
02­
26­
01
7:
54
P
DRIED
RED
MATERIAL
AROUND
LEFT
EYE
61476
F
1527
PPM
ORAL/
DENTAL
02­
26­
01
7~
55
P
DRIED
RED
MATERIAL
AROUND
MOUTH
61481
F
1527
PPM
NORMAL
02­
25­
01
8:
02
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61481
F
1527
PPM
DISPOSITION
02­
26­
01
7:
16
P
FOUND
DEAD
61484
F
1527
PPM
NORMAL
02­
25­
01
8:
02
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
61484
F
1527
PPM
DISPOSITION
02­
26­
01
13:
ll
P
SCHEDULED
EUTHANASIA
61484
F
1527
PPM
EYES/
EARS/
NOSE
02­
26­
01
7:
57
P
DRIED
RED
MATERIAL
AROUND
NOSE
02­
26­
01
7~
57
P
CLEAR
DISCHARGE
LEFT
EYE
02­
26­
01
7:
57
P
CLEAR
DISCHARGE
RIGHT
EYE
61484
F
1527
PPM
ORAL/
DENTAL
02­
26­
01
7:
5­
l
P
DRIED
RED
MATERIAL
AROUND
MOUTH
_______­___________­____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PCRW4.01
06/
18/
2001
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAp
TASK
FORCE
TABLE
14
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOSITIONS)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
27­
01
TO
02­
27­
01
PAGE
1
ANIMAL
SEX
GROUP
CATEGORY
__­______________­__­­­­­­­­­­­­­­­­­­­­­
61400
M
0
PPM
NORMAL
61400
M
0
PPM
DISPOSITION
61403
M
0
PPM
NORMAL
61403
M
0
PPM
DISPOSITION
61404
M
0
PPM
NORMAL
61404
M
0
PPM
DISPOSITION
61414
M
0
PPM
NORMAL
61414
M
0
PPM
DISPOSITION
61420
M
0
PPM
NORM?&
61420
M
0
PPM
DISPOSITION
61395
M
60/
45
PPM
NORMAL
61395
M
60/
45
PPM
DISPOSITION
61398
M
60/
45
PPM
NORMAL
61398
M
60/
45
PPM
DISPOSITION
61425
M
60/
45
PPM
NORMAL
61425
M
60145
PPM
DISPOSITION
61430
M
60/
45
PPM
NORMAL
61430
M
60145
PPM
DISPOSITION
61435
M
60/
45
PPM
NORMAL
61435
M
60/
45
PPM
DISPOSITION
61407
M
205/
177
PPM
NORMAL
61407
M
205/
177
PPM
DISPOSITION
61415
M
205/
177
PPM
NORMAL
61415
M
205/
177
PPM
DISPOSITION
61422
M
205/
17?
PPM
NORMAL
61422
M
205/
177
PPM
DISPOSITION
61424
M
205/
177
PPM
NORMAL
61424
M
205/
177
PPM
DISPOSITION
61433
M
2051177
PPM
NORMAL
DATE
TIME
GRADE
OBSERVATIONS
­­
­
­
­
___­­
.__­___­_________­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
02­
27­
01
8:
ll
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8~
12
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9~
20
P
SCHEDULED
EUTHANASIA
02­
27­
01
8~
12
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
13
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
13
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
13
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
14
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
a:
14
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
a:
15
P
No
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
15
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
16
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
16
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
28
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
17
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
29
P
SCHEDULED
EUTHANASIA
02­
27­
01
a:
17
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
27­
01
9:
29
P
SCHEDULED
EUTHANASIA
02­
27­
01
8:
18
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
__________­_D
E
______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
14
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPO.
YITIONS)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
27­
01
TO
02­
27­
01
PAGE
2
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
__­_____________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61433
M
61418
M
61418
M
61418
M
61418
M
205/
177
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
14741840
PPM
61418
M
1474/
840
PPM
61423
M
1474/
840
PPM
61423
M
1474/
840
PPM
61423
M
1474/
840
PPM
61423
M
1474/
840
PPM
61423
M
3.4741840
PPM
61428
M
1474/
840
PPM
61428
M
14741840
PPM
61428
M
1474/
840
PPM
61428
M
1474f840
PPM
61428
M
14741840
PPM
61428
M
1474/
840
PPM
61429
M
1474/
840
PPM
61429
M
1474/
840
PPM
61429
M
1474/
840
PPM
61429
M
1474/
840
PPM
61436
M
61436
M
1474/
840
PPM
1474/
840
PPM
DISPOSITION
DISPOSITION
BEHAVIOR/
CNS
BODY/
INTEGUMENT
EYES/
EARS/
NOSE
ORAL/
DENTAL
DISPOSITION
BEHAVIOR/
CNS
EYES/
EARS/
NOSE
EXCRETA
ORAL/
DENTAL
DISPOSITION
BEHAVIOR/
CNS
BODY/
INTEGUMENT
CARDIO­
PULMONARY
EYES/
EARS/
NOSE
EXCRETA
DISPOSITION
BEHAVIOR/
CNS
BODY/
INTEGUMENT
EYES/
EARS/
NOSE
DISPGSITION
BODY/
INTEGUMENT
02­
27­
01
9:
29
P
02­
27­
01
9:
29
P
02­
27­
01
8:
20
P
02­
27­
01
8:
lY
P
02­
27­
01
8:
18
P
02­
27­
01
8:
lY
P
02­
27­
01
8:
20
P
02­
27­
01
8:
19
P
02­
27­
01
9:
29
P
02­
27­
01
8:
22
P
02­
27­
01
8:
21
P
02­
27­
01
8:
21
P
02­
27­
01
8~
22
P
02­
27­
01
9:
29
P
02­
27­
01
8:
23
P
02­
27­
01
8~
23
P
02­
27­
01
8:
23
P
02­
27­
01
8:
23
P
02­
27­
01
8:
24
P
02­
27­
01
9:
29
P
02­
27­
01
8:
26
P
02­
27­
01
8:
25
P
02­
27­
01
a:
25
P
02­
27­
01
a:
25
P
02­
27­
01
9:
29
P
02­
7.7­
01
0:
27
P
SCHEDULED
EUTHANASIA
SCHEDULED
EUTHANASIA
LETHARGIC
BODY
COOL
TO
TOUCH
DRIED
RED
MATERIAL
AROUND
NOSE
CLEAR
DISCHARGE
RIGHT
EYE
CLEAR
DISCHARGE
LEFT
EYE
DRIED
RED
MATERIAL
AROUND
MOUTH
SCHEDULED
EUTHANASIA
LETHARGIC
DRIED
RED
MATERIAL
AROUND
NOSE
URINE
BROWNISH
RED
IN
COLOR
DRIED
RED
MATERIAL
AROUND
MOUTH
SCHEDULED
EUTHANASIA
LETHARGIC
BODY
COOL
TO
TOUCH
RESPIRATION
RATE
DECREASED
DRIED
RED
MATERIAL
AROUND
NOSE
URINE
BROWNISH
RED
IN
COLOR
SCHEDULED
EUTHANASIA
LETHARGIC
BODY
COOL
TO
TOUCH
CLEAR
DISCHARGE
LEFT
EYE
DRIED
RED
MATERIAL
AROUND
NOSE
SCHEDULED
EUTHANASIA
BODY
COOL
TO
TOUCH
_____________­______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
_­­_­­­__­_­­­­­­­­­­­­­­
ANIMAL
SEX
GROUP
61450
F
0
PPM
61450
F
0
PPM
61464
F
0
PPM
61464
F
0
PPM
61469
F
0
PPM
61469
F
0
PPM
61490
F
0
PPM
61490
F
0
PPM
61494
F
0
PPM
61494
F
0
PPM
61454
F
60145
PPM
61454
F
60/
45
PPM
61457
F
60145
PPM
61457
F
60/
45
PPM
61466
F
60/
45
PPM
61466
F
60/
45
PPM
61487
F
60/
45
PPM
61487
F
60/
45
PPM
61492
F
60/
45
PPM
61492
F
60/
45
PPM
61448
F
2051177
PPM
61448
F
205/
177
PPM
61463
F
205/
177
PPM
61463
F
205/
177
PPM
61468
F
205/
177
PPM
61468
F
205/
177
PPM
61483
F
205/
177
PPM
61483
F
205/
177
PPM
61489
F
205/
177
PPM
­­­,
TABLE
14
(DETAILED
PHYSICAL
EXAMINATI~
NS/
DISPO~
ITIONS)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
28­
01
TO
02­
28­
01
PAGE
3
­­__
CATEGORY
­­__­­­­­­­­­­
NORMAL
DISPOSITION
NORMAL
DISWSITION
NORM&
L
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DISPOSITION
NORMAL
DATE
TIME
GRADE
OBSERVATIONS
,___­___­_____­­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
02­
28­
01
7:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHT+
NIZATION
02­
28­
01
7:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
32
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
32
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
33
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
33
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
34
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
08
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
34
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
09
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
35
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
28­
01
lo:
09
P
SCHEDULED
EUTHANIZATION
02­
28­
01
7:
35
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
________________________________________­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
______________­____________
ANIMAL
SEX
GROUP
_______________­____­­­­­­­
61489
61445
61445
61445
61460
61460
61460
61460
61460
61478
61478
63.478
61478
61478
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
61485
F
1474/
840
PPM
61485
F
1474/
840
PPM
205/
177
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
__

­­
TABLE
14
(DETAILED
PHYSICAL
EXAMINATIONS/
DISPOS3TIONS)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
FATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
28­
01
TO
02­
28­
01
PAGE
4
.______________­____­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
.___________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­
DISPOSITION
02­
28­
01
lo:
09
P
SCHEDULED
EUTHANIZATION
DISPOSITION
02­
28­
03.
lo:
09
P
SCHEDULED
EUTHANIZATION
BODY/
INTEGUMENT
02­
28­
01
7~
38
P
BODY
COOL
TO
TOUCH
EYES/
EARS/
NOSE
02­
28­
01
7:
39
P
DRIED
RED
MATERIAL
AROUND
LEFT
EYE
02­
28­
01
7:
40
P
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
DISPOSITION
02­
28­
01
9:
32
P
EUTHANIZED
IN
EXTREMIS
BEHAVIOR/
CNS
02­
28­
01
7:
41
P
LETHARGIC
02­
28­
01
7:
42
P
ATAXIA
BODY/
INTEGUMENT
02­
28­
01
7:
41
P
BODY
COOL
TO
TOUCH
CARDIO­
PULMONARY
02­
28­
01
7:
41
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
28­
01
7:
42
P
CLEAR
DISCHARGE
RIGHT
EYE
DISPOSITION
02­
28­
01
8:
17
P
FOUND
DEAD
BEHAVIOR/
CNS
02­
28­
01
7:
43
P
PROSTRATE
BODY/
INTEGUMENT
02­
28­
01
7:
44
P
BODY
COOL
TO
TOUCH
CARDIO­
PULMONARY
02­
28­
01
7~
44
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
28­
01
7:
44
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
28­
01
7:
44
P
CLEAR
DISCHARGE
LEFT
EYE
NORMAL
02­
28­
01
7:
46
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
DISPOSITION
02­
28­
01
lo:
09
P
SCHEDULED
EUTHANIZATION
co
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PCRDv4.01
06/
12/
2001
R:
O6/
12/
2001
PAGE
1
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
15
(IMMEDIATELY
FOLLOWING
EXPOSURE)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
25­
01
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
_________­­_________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
61409
M
0
PPM
61410
M
0
PPM
61419
M
0
PPM
61432
M
0
PPM
61434
M
0
PPM
61396
M
5%
PPM
61405
M
58
PPM
61413
M
58
PPM
61416
M
58
PPM
61431
M
58
PPM
61399
M
181
PPM
61411
M
181
PPM
61441
M
181
PPM
61442
M
181
PPM
61443
M
181
PPM
61397
M
1527
PPM
61397
M
1527
PPM
61397
M
1527
PPM
61406
M
61406
M
61406
M
1527
PPM
1527
PPM
1527
PPM
61408
M
61408
M
61408
M
1527
PPM
1527
PPM
1527
PPM
61421
M
61421
M
1527
PPM
1527
PPM
NORMAL
02­
25­
01
13:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
25
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
25
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
26
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
26
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
27
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
15
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
15
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
15
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
16
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
16
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
BEHAVIOR/
CNS
02­
25­
01
13:
42
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
42
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
43
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
43
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
43
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
44
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
44
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
44
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
44
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
44
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
44
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
45
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
45
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
45
P
RESPIRATION
RATE
DECREASED
___­_____­____­­________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PAGE
2
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
15
(IMMEDIATELY
FOLLOWING
EXPOSURE1
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOOY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
25­
01
____________­_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
_____­______­_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
61421
M
1527
PPM
61439
M
61439
M
61439
M
1527
PPM
1527
PPM
1527
PPM
61446
F
61452
F
61453
F
61456
F
61491
F
61467
F
61470
F
61472
F
61475
F
61417
F
61449
F
61474
F
61482
F
61486
F
61493
F
61451
F
61451
F
61451
F
0
PPM
0
PPM
0
PPM
0
PPM
0
PPM
58
PPM
50
PPM
58
PPM
58
PPM
58
PPM
181
PPM
181
PPM
181
PPM
181
PPM
181
PPM
1527
PPM
3.527
PPM
1527
PPM
61462
F
61462
F
61462
F
1527
PPM
1527
PPM
1527
PPM
EYES/
EARS/
NOSE
02­
25­
01
13:
45
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
45
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
45
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
46
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
46
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13~
46
P
CLEAR
DISCHARGE
LEFT
EYE
NORMAL
02­
25­
01
13:
30
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
31
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAG
02­
25­
01
13:
27
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
13:
29
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
16
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORMAL
02­
25­
01
14:
17
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NORM&
L
02­
25­
01
14:
17
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
EYES/
EARS/
NOSE
02­
25­
01
14:
18
P
DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
NORMAL
02­
25­
01
14:
18
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
BEHAVIOR/
CNS
02­
25­
01
13:
47
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
47
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
47
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
47
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
47
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
48
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
48
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
48
P
CLEAR
DISCHARGE
LEFT
EYE
_______________­________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
NIL­
417001
SPONS0R:
HA.
P
TASK
FORCE
TABLE
15
(IMMEDIATELY
FOLLOWING
EXPOSURE)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
TO
02­
25­
01
PAGE
3
____________"_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
61476
F
1527
PPM
61476
F
1527
PPM
61476
F
1527
PPM
61481
F
1527
PPM
61481
F
1527
PPM
61481
F
1527
PPM
61484
F
1527
PPM
61484
F
1527
PPM
61484
F
1527
PPM
__­­­­­­­­­­__­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
r
­_­
BEHAVIOR/
CNS
02­
25­
01
13:
49
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
49
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
49
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
49
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
49
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
49
P
RESPIRATION
RATE
DECREASED
EYES/
EARS/
NOSE
02­
25­
01
13:
49
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
49
P
CLEAR
DISCHARGE
LEFT
EYE
BEHAVIOR/
CNS
02­
25­
01
13:
49
P
ANIMAL
SLEEPING
CARDIO­
PULMONARY
02­
25­
01
13:
50
P
RESPIRATION
RATE
DECREASED
EYES/
BARS/
NOSE
02­
25­
01
13:
50
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
25­
01
13:
50
P
CLEAR
DISCHARGE
LEFT
EYE
MODERATE
3
­
SEVERE
P
­
PRESENT
PCRDv4.04
10/
29/
2001
PROJECT
NO.:
WIL­
417001A'
SPONSOR:
HAP
TASK
FORCE
TABLE
15
(IMMEDIATELY
FOLLOWING
EXPOSURE)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLGGY~
STUDY
OF
1.1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
26­
01
TO
02­
26­
01
PAGE
I
________­_________­_____________
I_______­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEGORY
DATE
TIME
GRADE
OBSERVATIONS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~~­­­­­­­­­
61400
M
0
PPM
61403
M
0
PPM
61404
M
0
PPM
61414
M
0
PPM
61420
M
0
PPM
61395
M
60/
45
PPM
61398
M
60/
45
PPM
61425
M
60/
45
PPM
61430
M
60/
45
PPM
61435
M
60/
45
PPM
61407
M
205/
177
PPM
61415
M
205/
177
PPM
61422
M
2051177
PPM
61424
M
205/
171
PPM
61433
M
2051177
PPM
61418
M
14741840
PPM
61423
M
1474/
840
PPM
61423
M
61428
M
61428
M
61429
M
61429
M
61436
M
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
1474/
840
PPM
14741840
PPM
14741840
PPM
61436
M
1474/
840
PPM
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
NORMAL
BEHAVIOR/
CNS
BEHAVIOR/
CNS
EYES/
EARS/
NOSE
BEHAVIOR/
CNS
CARDIO­
PULMONARY
REHAVIQR/
CNS
EYES/
EARS/
NOSE
BEUAVIOR/
CNS
EYES/
EARS/
NOSE
02­
26­
01
11:
05
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
06
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
07
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
08
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
08
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
26
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
26
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
27
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
27
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
28
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
41
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
41
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
42
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
42
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
43
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
02­
26­
01
11:
50
P
LETHARGIC
02­
26­
01
11:
52
P
LETHARGIC
02­
26­
01
11~
53
P
ATAXIA
02­
26­
01
11:
54
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
26­
01
11:
55
P
ANIMAL
SLEEPING
02­
26­
01
11:
56
P
RESPIRATION
RATE
DECREASED
02­
26­
01
11:
56
p
ANIMAL
SLEEPING
02­
26­
01
11:
57
P
CLEAR
DISCHARGE
LEFT
EYE
02­
26­
01
11:
58
P
LETHARGIC
02­
26­
01
11:
58
P
ATAXIA
02­
26­
01
11:
58
P
CLEAR
DISCHARGE
RIGHT
EYE
02­
26­
01
11:
58
P
CLEAR
DISCHARGE
LEFT
EYE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
15
(IMMEDIATELY
FOLLOWING
EXPOSURE)
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
27­
01
TO
02­
27­
01
PAGE
2
__­______"______________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
CATEG0RY
DATE
TIME
GRADE
OBSERVATIONS
________­________­__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61450
F
0
PPM
61464
F
0
PPM
61469
F
0
PPM
61490
F
0
PPM
61494
F
0
PPM
61454
F
60/
45
PPM
61457
F
60/
45
PPM
61466
F
60145
PPM
61487
F
60/
45
PPM
61492
F
60/
45
PPM
61448
F
2051177
PPM
61463
F
205/
177
PPM
61468
F
205/
177
PPM
61483
F
2051177
PPM
61489
F
2051177
PPM
61445
F
1474/
840
PPM
61445
F
1474/
840
PPM
61445
F
14741840
PPM
61460
F
61460
F
61460
F
1474/
840
PPM
14741840
PPM
1474/
840
PPM
61478
F
61478
F
61478
F
14741840
PPM
1474/
840
PPM
14741040
PPM
61485
F
61485
F
1414/
040
PPM
14741840
PPM
NORMAL
02­
27­
01
12:
25
P
NORMAL
02­
27­
01
12:
25
P
NORMAL
02­
27­
01
12:
26
P
NORMAL
02­
27­
01
12:
26
P
NORMAL
02­
27­
01
12:
26
P
NORMAL
02­
27­
01
12:
32
P
NORMAL
02­
27­
01
12:
32
P
NORMAL
.
02­
27­
01
12:
32
P
NORMAL
02­
27­
01
12:
33
P
NORMAL
02­
27­
01
12:
33
P
NORMAL
02­
27­
01
12:
35
P
NORMAL
02­
27­
01
12:
36
P
NORMAL
02­
27­
01
12:
36
P
NORMAL
02­
27­
01
12:
37
P
NORMAL
02­
27­
01
12:
37
P
BEHAVIOR/
CNS
02­
27­
01
13:
02
P
CARDIO­
PULMONARY
02­
27­
01
13:
02
P
EYES/
EARS/
NOSE
02­
27­
01
13:
03
P
02­
27­
01
13:
03
P
BEHAVIORfCNS
02­
27­
01
13:
09
P
CARDIO­
PULMONARY
02­
27­
01
13:
OP
P
EYES/
EARS/
NOSE
02­
27­
01
13:
l.
O
P
02­
27­
01
13:
lO
P
BEHAVIOR/
CNS
02­
27­
01
13:
lO
P
CARDIO­
PULMONARY
02­
27­
01
13:
lO
P
EYES/
EARS/
NOSE
02­
27­
01
13:
lO
P
02­
27­
01
13:
lO
P
BEHAVIOR/
CNS
02­
27­
01
13:
ll
P
CARDIO­
PULMONARY
02­
27­
01
13:
ll
P
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
No
SIwIFImiT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
ANIMAL
SLEEPING
RESPIRATION
RATE
DECREASED
CLEAR
DISCHARGE
RIGHT
EYE
CLEAR
DISCHARGE
LEFT
EYE
ANIMAL
SLEEPING
RESPIRATION
RATE
DECREASED
CLEAR
DISCHARGE
RIGHT
EYE
CLEAR
DISCHARGE
LEFT
EYE
ANIMAL
SLEEPING
RESPIRATION
RATE
DECREASED
CLEAR
DISCHARGE
RIGHT
EYE
CLEAR
DISCHARGE
LEFT
EYE
ANIMAL
SLEEPING
RESPIRATION
RATE
DECREASED
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­

GRADE
CODE:
1
­
SLIGHT
2
­
MODERATE
3
­
SEVERE
P
­
PRESENT
115
PROJECT
NO.:
WIL­
417001
SPONS0R:
HA.
P
TASK
FORCE
TABLE
16
(RESPONSE
TO
NOVEL
STIMULUS)
ACUTE
INH.
TOX.
(WITH
HIST~
PATHOLOGY)
STUDY
OF
l,
l,
z­~
C~
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
PAGE
1
ANIMAL
SEX
GROUP
DATE
TIME
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61409
M
61410
M
61419
M
61432
M
61434
M
61396
M
61405
M
61413
M
61416
M
61431
M
61399
M
61411
M
61441
M
61442
M
61443
M
61397
M
61406
M
61408
M
61421
M
61439
M
0
PPM
02­
25­
01
14:
15
0
PPM
02­
25­
01
14:
15
0
PPM
02­
25­
01
14:
15
0
PPM
02­
25­
01
14:
15
0
PPM
02­
25­
01
14:
15
58
PPM
02­
25­
01
14:
18
58
PPM
02­
25­
01
14:
18
58
PPM
02­
25­
01
14:
lE
58
PPM
02­
25­
01
14:
18
50
PPM
02­
25­
01
14:
18
181
PPM
02­
25­
01
14:
22
181
PPM
02­
25­
01
14:
22
181
PPM
02­
25­
01
14:
22
181
PPM
02­
25­
01
14:
22
181
PPM
02­
25­
01
14:
22
1527
PPM
02­
25­
01
14:
25
1527
PPM
02­
25­
01
14:
25
1527
PPM
02­
25­
01
14:
25
1527
PPM
02­
25­
01
14:
25
1527
PPM
02­
25­
01
14:
25
OBSERVATIONS
_­­­__­_____________­­­­­­­­­­­­
NOTABLY
INCREASED
REACTION
NO
REACTION
TO
STIMULUS
NOTABLY
INCREASED
REACTION
NOT
VISIBLE
NO
REACTION
TO
STIMULUS
NOTABLY
INCREASED
REACTION
SLIGHT
REACTION
NOTABLY
INCREASED
REACTION
NOT
VISIBLE
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
SLIGHT
REACTION
SLIGHT
REACTION
NOT
VISIBLE
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
No
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NOT
VISIBLE
NO
REACTION
TO
STIMULUS
TABLE
16
(RESPONSE
~0
NOVEL
STIMULUS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
2
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
25­
01
_____________"__________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­

ANIMAL
SEX
GROUP
DATE
TIME
OBSERVATIONS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­"­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­

61446
F
0
PPM
02­
25­
01
14:
15
NO
REACTION
TO
STXMULUS
61452
F
0
PPM
02­
25­
01
14:
lS
NOTABLY
INCREASED
REACTION
61453
F
0
PPM
02­
25­
01
14:
15
NO
REACTION
TO
STIMULUS
61456
F
0
PPM
02­
25­
01
14:
15
NO
REACTION
TO
STIMULUS
61491
F
0
PPM
02­
25­
01
14:
15
NOT
VISIBLE
61467
F
58
PPM
02­
25­
01
14:
18
NO
REACTION
TO
STIMULUS
61470
F
58
PPM
02­
25­
01
14:
18
NOTABLY
INCREASED
REACTION
61472
F
58
PPM
02­
25­
01
14:
18
NO
REACTION
TO
STIMULUS
61475
F
58
PPM
02­
25­
01
14:
18
NOTABLY
INCREASED
REACTION
61477
F
58
PPM
02­
25­
01
14:
18
NOT
VISIBLE
61449
F
181
PPM
02­
25­
01
14:
22
NO
REACTION
TO
STIMULUS
61474
F
181
PPM
02­
25­
01
14:
22
SLIGHT
REACTION
61482
F
181
PPM
02­
25­
01
14:
22
SLIGHT
REACTION
61486
F
181
PPM
02­
25­
01
14:
22
NO
REACTION
TO
STIMULUS
61493
F
181
PPM
02­
25­
01
14:
22
NOT
VISIBLE
61451
F
1527
PPM
02­
25­
01
14:
25
NO
REACTION
TO
STIMULUS
61462
F
1527
PPM
02­
25­
01
14:
25
NO
REACTION
TO
STIMULUS
61476
F
1527
PPM
02­
25­
01
14:
25
NO
REACTION
TO
STIMULUS
61481
F
1527
PPM
02­
25­
01
14~
25
NO
REACTION
TO
STIMULUS
61484
F
1527
PPM
02­
25­
01
14:
25
NOT
VISIBLE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

MANuALv1.0
06/
20/
2001
R:
06/
20/
2001
I
fi:
00
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
16
(RESPONSE
TO
NOVEL
STIMULUS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
26­
01
PAGE
1
ANIMAL
SEX
GROUP
DATE
TIME
OBSERVATIONS
________­____­
­­­­­­­­­­­­­­­­­­­­­­­­­­­­.
­
­
­
­
­
­
­
_______­­_______________________________­­­­­­­­­­­­­­­­­­­­­­­­­
61400
M
0
PFM
02­
26­
01
14:
16
SLIGHT
REACTION
61403
M
0
PPM
02­
26­
01
14:
16
NOTABLY
INCREASED
REACTION
61404
M
0
PPM
02­
26­
01
14:
16
SLIGHT
REACTION
61414
M
0
PPM
02­
26­
01
14:
16
NOTABLY
INCREASED
REACTION
61420
M
0
PPM
02­
26­
01
14:
16
NOT
VISIBLE
61395
M
60
PPM
02­
26­
01
14:
21
NO
REACTION
TO
STIMULUS
61398
M
60
PPM
02­
26­
01
14:
21
NOTABLY
INCREASED
REACTION
61425
M
60
PPM
02­
26­
01
14:
21
SLIGHT
REACTION
61430
M
60
PPM
02­
26­
01
14:
21
SLIGHT
REACTION
61435
M
60
PPM
02­
26­
01
14:
21
NOT
VISIBLE
61407
M
205
PPM
02­
26­
01
14:
24
SLIGHT
REACTION
61415
M
205
PPM
02­
26­
01
14:
24
NO
REACTION
TO
STIMULUS
61422
M
205
PPM
02­
26­
01
14:
24
SLIGHT
REACTION
61424
M
205
PPM
02­
26­
01
14:
24
NO
REACTION
TO
STIMULUS
61433
M
205
PPM
02­
26­
01
14:
24
NO
REACTION
TO
STIMULUS
61418
M
1474
PPM
02­
26­
01
*
NO
REACTION
TO
STIMULUS
61423
M
1474
PPM
02­
26­
01
*
NO
REACTION
TO
STIMULUS
61428
M
1474
PPM
02­
26­
01
l
NO
REACTION
T0
STIMULUS
61429
M
1474
PPM
02­
26­
01
*
NO
REACTION
TO
STIMULUS
61436
M
2.474
PPM
02­
26­
01
*
NO
REACTION
TO
STIMULUS
______________________________
r_________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
*
=
TIME
INADVERTENTLY
NOT
RECORDED
_____­_­_______­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
PROJECT
NO.:
WILr417001A
SPONSOR:
HAP
TASK
FORCE
___________­_­­_
I­__­­­­­­­­­

ANIMAL
SEX
GROUP
_____________­­__­__­­­­­­­­­

61450
F
61464
F
61469
F
61490
F
61494
F
61454
F
61457
F
61466
F
61487
F
61492
F
61448
F
61463
F
61468
F
61483
F
61469
F
61445
F
63.458
F
61460
F
61478
F
61485
F
___­_­_­­­__
0
PPM
02­
27­
01
14:
OS
0
PPM
02­
27­
01
14:
OS
0
PPM
02­
27­
01
14:
05
0
PPM
02­
27­
01
14:
OS
0
PPM
02­
27­
01
14:
OS
45
PPM
02­
27­
01
14:
08
45
PPM
02­
27­
01
14:
08
45
PPM
02­
27­
01
14:
08
45
PPM
02­
27­
01
14:
08
45
PPM
02­
27­
01
14:
08
177
PPM
02­
27­
01
14:
12
177
PPM
02­
27­
01
14:
12
177
PPM
02­
27­
01
14:
12
177
PPM
02­
27­
01
14:
12
177
PPM
02­
27­
01
14:
12
527
PPM
02­
27­
01
14:
18
840
PPM
02­
27­
01
14:
18
840
PPM
02­
27­
01
14:
18
840
PPM
02­
27­
01
14:
18
840
PPM
02­
27­
01
14:
18
__.

­­.
TABLE
16
(RESPONSE
TO
NOVEL
STIMULUS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
CLINICAL
OBSERVATIONS
TABLE
RANGE:
02­
27­
01
PAGE
2
____­___­______­­_______________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
DATE
TIME
OBSERVATIONS
_­_________­­___________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

SLIGHT
REACTION
NOTABLY
INCREASED
REACTION
NO
REACTION
TO
STIMULUS
NOTABLY
INCREASED
REACTION
NO
REACTION
TO
STIMULUS
SLIGHT
REACTION
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
SLIGHT
REACTION
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
NO
REACTION
TO
STIMULUS
____­­____­_____________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MANuALv1.0
06,
'20/
2001
R:
10/
29/
2001
S
S
b'B1
E'
Pt
`TRK
`8L1:

`66K
`961
'
16'1:
'981
`LLT.
`SLT
`fLT
`ELt
.
E9K
8SI
_­_­______­_________­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­

K
0
hldd
0
:dflOW
3?
tfW
1
33'fd
(Sm3)
sum3t­
i
xaoa
wnarfiraN1
SUi­
tl
NI
33eL­
Z'I'T
JO
hanJS
(ACXTIOH~
VdOLSIH
HLIM)
'XOZ
'HNI
3.
LKW
L­
t
3Tw.
u
S
9'OK
'
09­
t
.
PLT
.
L91:
'LSf
'851
'9bK
E­
S
9'6
.
L.?
x
'6ET
'ZEK
'SZK
82X
.
EK.
'I:

.­­­­­.
6­
N
'a's
NY3N
PEP19
ZEbT9
6K619
OTPK9
60PT9
?tlhlINV
hQCl
TABLE
17
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1.1,2­
TCE
IN
RATS
PAGE
2
SPONSOR:
H?
iP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)

MALE
GROUP:
58
PPM
DAY
­9
­3
0
1
___________________­____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ANIMAL
61396
133.
166.
181.
180.
61405
125.
160.
174.
178.
61413
113.
143.
157.
160.
61416
137.
175.
197.
197.
61431
124.
152.
167.
169.

MEAN
126.
159.
175.
177.
S.
D.
9.3
12.4
15.1
13.8
N
5
5
5
5
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
­9
____­­­­­­­­
DAY
­­­­­­_­­­­
ANIMAL
61399
61411
61441
61442
61443
MALE
GROUP:
181
PPM
­3
0
1
_________­­­_­­__­­­__________________
I_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­

124.
157.
173.
165.
127.
161.
179.
172.
128.
162.
182.
177.
132.
169.
186.
178.
115.
148.
167.
167.

MEAN
125.
159.
177.
172.
S.
D.
6.4
7.7
7.5
5.8
N
5
5
5
5
TABLE
17
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)
PAGE
3
123
TABLE
17
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOL~
Y)
STUDY
OF
~,~,
Z­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIIJUAL
BODY
WEIGHTS
(GRAMS)
PAGE
5
FEMALE
GROUP:
0
PPM
DAY
­9
­3
0
1
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61446
108.
119.
130.
132.
61452
105.
121.
X28.
130.
61453
100.
109.
115.
114.
61456
100.
114.
121.
122.
61491
105.
124.
129.
130.

MEAN
104.
117.
125.
126.
S.
D.
3.5
5.9
6.4
7.5
N
5
5
5
5
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
17
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)
PAGE
6
FEMALE
GROUP:
58
PPM
DAY
­9
­3
0
1
______________________________________
L_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61467
95.
111.
115.
117.

61470
100.
112.
118.
119.
61472
102.
121.
129.
131.
61475
108.
122.
132.
130.
61477
99.
116.
126.
127.

MEAN
101.
116.
12.4.
125.

S.
D.
4.8
5.0
7.2
6.4
N
5
5
5
5
I.
26
I.
27
,
,
I
I
,
I
t
,
I
I
/
2
/
I
/
I
,
I
,
/
I
,
,
1
I
,
i
,
,
,

rl
w
H
128
129
TABLE
17
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HIST~
PATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)
PAGE
3
­
­
_
_
­
­
­
_
_
ANIMAL
61407
61415
61422
61424
61433
­9
_­­___­­­_­­
­3
­__­"_____­­­

124.
154.
134.
168.
115.
148.
133.
172.
122.
158.

MEAN
126.
160.
S.
D.
8.0
9.9
N
5
5
MALE
GROUP:
205/
177
PPM
0
1
.______­______­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

171.
166.
190.
184.
171.
165.
197.
190.
186.
179.

183.
177.
11.6
11.0
5
5
TABLE
17
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HIST~
PATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)
PAGE
4
MALE
GROUP:
14741840
PPM
DAY
­9
­3
0
1
____________________
L___________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61418
124.
160.
182.
159.
61423
128.
162.
185.
160.
61428
119.
152.
178.
152.
61429
141.
173.
200.
173.
61436
119.
148.
173.
149.

MEAN
126.
159.
184.
159.
S.
D.
9.1
9.7
10.2
9.3
N
5
5
5
5
__­.­_
­
_­­__
­
­__­­­
­
_­­___­__­­­­­­­­_
TABLE
17
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)
PAGE
5
FEMALE
GROUP:
0
PPM
DAY
­9
­3
0
1
__________________­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61450
109.
125.
135.
136.
61464
93.
111.
124.
124.
61469
96.
112.
122.
123.
61490
102.
116.
131.
132.
61494
105.
119.
131.
133.

ME?.
N
101.
117.
129.
130.
S.
D.
6.5
5.7
5.4
5.8
N
5
5
5
5
TABLE
17
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HI.
STOPATHOLO(;
Y)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)

FEMALE
GROUP:
60/
45
PPM
DAY
­9
­3
0
1
________________________________________­­­­­­~­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61454
109.
127.
144.
139.
61457
98.
110.
116.
118.
61466
104.
118.
132.
127.
61487
107.
121.
134.
132.
61492
94.
112.
124.
123.

MEAN
102.
118.
130.
128.
S.
D.
6.3
6.9
10.6
8.1
N
5
5
5
5
PAGE
6
PAGE
7
TABLE
17
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRAMS)

FEMALE
GROUP:
205/
111
PPM
DAY
­3
­3
0
1
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61448
110.
121.
134.
129.

61463
92.
113.
125.
115.

61468
33.
107.
119.
112.

61483
114.
127.
138.
132.

61489
104.
118.
129.
121.

MERN
103.
117.
129.
122.

S.
D.
3.3
7.6
1.4
8.6
N
5
5
5
5
TABLE
17
PROJECT
NO.:
WIL­
41700J.
A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHTS
(GRnMS)
PAGE
8
FEMALE
GROUP:
1474/
840
PPM
DAY
­9
­3
0
1
___________
s____________________________­____________________________­__­­_________"________
I__._______________________­__­­­­­~­­­­

ANIMAL
61445
105.
119.
131.
119.
61458
96.
114.
126.
NA
61460
105.
119.
132.
117.
61478
108.
122.
133.
121.
61485
98.
110.
118.
108.

MEAN
102.
117.
128.
116.
S.
D.
5.1
4.8
6.2
5.7
N
5
S
5
4
NA
=
NOT
APPLICABLE
PBFTSv4.13
06/
12/
2001
136
138
139
TABLE
18
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
5
FEMALE
GROUP:
0
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
_________________­______________
c_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ANIMAL
61446
11.
11.
2.
61452
16.
7.
2.
61453
9.
6.
­1.
61456
14.
7.
1.
61491
19.
5.
1.

MEAN
14.
7.
1.
S.
D.
4.0
2.3
1.2
N
5
5
5
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
18
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
6
FEMALE
GROUP:
58
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
_______________"____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61467
16.
4.
2.
61470
12.
6.
1.
61472
3.9.
8.
2.
61475
14.
10.
­2.
61477
17.
10.
1.

MEAN
16.
8.
1.
S.
D.
2.7
2.6
1.6
N
5
5
5
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
18
ACUTE
INH.
TAX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
7
FEMALE
GROUP:
181
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
__"________
s____________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­

ANIMAL
61449
13.
11.
61474
12.
3.
61482
15.
9.
61486
17.
7.
61493
17.
7.
­7.
­3.
­5.
­7.
­6.

MEAN
15.
7.
­6.
S.
D.
2.3
3.0
1.7
N
5
5
5
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
DAY
­9
TO
­3
­3T0
0
____________________­­­­­­­­­­­

ANIMAL
61451
19.
7.
61462
12.
10.
61476
14.
8.
61481
12.
9.
61484
13.
8.

MEAN
14.
8.
S.
D.
2.9
1.1
N
5
5
NA
=
NOT
APPLICABLE
TABLE
18
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
0
FEMALE
GROUP:
1527
PPM
OTO
1
_______^____­____­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­

NA
­12.
­12.
NA
­16.

­13.
2.3
3
PBFTSv4.13
06/
12/
2001
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
18
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
1
MALE
GROUP:
0
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
_­______­__­____________________________­­­­­­­­­­­"­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­.~­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61400
29.
24.
­1.
61403
36.
20.
1.
61404
30.
19.
2.
61414
29.
20.
0.
61420
4l..
25.
0.

MEAN
33.
22.
0.
S.
D.
5.3
2.7
1.1
N
5
5
5
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RnTS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
2
MALE
GROUP:
60/
45
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­.­­­­­­­­­­­~­­­­­­­­­­­­­­­­­

ANIMAL
61395
36.
61398
32.
61425
32.
61430
31.
61435
37.
19.
­1.
17.
­1.
20.
1.
22.
0.
26.
­6.

MEAN
34.
21.
­1.
S.
D.
2.1
3.4
2.1
N
5
5
5
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
3
MALE
GROUP:
205/
177
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­

ANIMAL
61407
30.
17.
­5.
61415
34.
22.
­6.
61422
33.
23.
­6.
61424
39.
25.
­7.
61433
36.
28.
­7.

MEAN
34.
23.
­6.
S.
D.
3.4
4.1
0.8
N
5
5
5
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
4
MALE
GROUP:
1474/
840
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
________________________________________­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61418
36.
22.
­23.
61423
34.
23.
­25.
61428
33.
26.
­26.
61429
32.
27.
­27.
61436
29.
25.
­24.

MEAN
33.
25.
­25.

S.
D.
2.6
2.1
1.6
N
5
5
5
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)
PAGE
5
DAY
­9
TO
­3
_____________­­­­­­_
ANIMAL
61450
16.
61464
18.
61469
16.
61490
14.
61494
14.
FEMALE
GROUP:
0
PPM
­3T0
0
OTO
1
________
L___________­­­­­­­­­­­­­­­­­­­­­­­­­
________­­­_________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

10.
1.
13.
0.
10.
1.
15.
1.

12.
2.

MEAN
16.
12.
1.
S.
D.
1.7
2.1
0.7
N
5
5
5
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INA.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)

FEMALE
GROUP:
60/
4S
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ANIMAL
61454
18.
17.
­5.
61457
12.
6.
2.
61466
14.
14.
­5.
61487
14.
13.
­2.
61492
18.
12.
­1.

MEAN
15.
12.
­2.
S.
D.
2.7
4.0
2.9
N
5
5
5
PAGE
6
,

150
TABLE
18
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATKOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BODY
WEIGHT
GAINS
(GRAMS)

FEMALE
GROUP:
1474/
840
PPM
DAY
­9
TO
­3
­3T0
0
OTO
1
________________________________________­­­­­­­­­­­­­­­­­­­­
______­_____________­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
61445
14.
12.
­12.
61458
18.
12.
NA
61460
14.
13.
­15.
61478
14.
11.
­12.
61485
12.
8.
­10.

MEAN
14.
11.
­12.
S.
D.
2.2
1.9
2.1
N
5
5
4
NA
=
NOT
APPLICABLE
PAGE
8
PBFTSv4.13
06/
12/
2001
PROJECT
NO.:
WIL­
417001A
SPONSOR
:
:HAP
TASK
FORCE
TABLE
19
ACUTE
INH.
TOX
(WITH
HISTOPATHOLCGY)
STUDY
OF
l,
l,
z­
TIE
IN
RATS
INDIVIDUAL
BRONCHOALVEOLAR
LAVAGE
VALUES
PAGE
1
DAY
1
_______­________________­_­_____________­­__­_­_­­_____­­__
r_________­­_______­____­­_­___­­­­________________,
r_
l­_________________

TOTAL
TVOL
TOTALCEL
ANIMAL
PROTEIN
COUNT
__­_________________­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

mg/
dL
nfL
x10­
t/
ml,
________"____­___________~~~_____________________________________­­­__________________­­_­_________"_____
r_________________________
GROUP:
0
PPM
MALES
61400
a.
4
4.
1.5
61403
7.9
3.
1.7
61404
9.0
4.
2.3
61414
a.
0
4.
1.7
61420
10.3
3.
1.4
MEAN
a.
7
4.
1.7
S.
D.
0.98
0.5
0.35
N
5
5
5
________­­­______________________
L______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
mg/
dL
=
GRAMS/
DECILITER,
II&
=
MILLILITERS
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
19
ACUTE
INH.
TOX
(WITH
HISTOPATHOLCGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
INDIVIDUAL
BRONCHOALVEOLAR
LAVAGE
VALUES
PAGE
2
DAY
1
_____________­______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
TOTAL
TVOL
TOTALCEL
ANIMAL
PROTEIN
COUNT
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

w/
dL
mL
x10­
6/
11&
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
GROUP:
60/
45
PPM
MALES
61395
LA
LA
LA
61398
12.0
3.
1.9
61425
8.6
4.
1.4
61430
LA
LA
LA
61435
10.0
3.
0.9
MEAN
10.2
3.
1.4
S.
D.
1.71
0.6
0.50
N
3
3
3
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
r
LA
=
LAB
ACCIDENT
________­_______________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
­­­­­­­­
mg/
dL
=
GRAMS/
DECILITER,
mL
=
MILLILITERS
TABLE
19
PROJECT
NO.:
WIL­
417001A
ACUTE
INH.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
3
SPONSOR:
HAP
TASK
FORCE
INDIVIDUAL
BRONCHOALVEOLAR
LAVAGE
VALUES
DAY
1
____________________
I___________________­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

TOTAL
TVOL
TOTALCEL
ANIMAL
PROTEIN
COUNT
__________________
L_____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

w/
dL
mu
%10"
6/
mL
_________­______"_______________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GROUP:
205/
177
PPM
MALES
61407
13.8
2.
1.8
61415
LA
LA
LA
61422
8.1
4.
1.6
61424
9.5
4.
1.5
61433
9.7
4.
1.2
MEAN
10.3
4.
1.5
S.
D.
2.46
1.0
0.25
N
4
4
4
­­­­­­­­­­­­­_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~
LA
=
LAB
ACCIDENT
mg/
dL
=
GRAMS/
DECILITER,
mL
=
MILLILITERS
PROJECT
NO.:
WIL­
417001A
SPONSOR:
HAP
TASK
FORCE
TABLE
19
ACUTE
INN.
TOX
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
INDIVIDUAL
BRONCHOALVEOLAR
LAVAGE
VALUES
PAGE
4
DAY
1
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

TOTAL
TVOL
TOTALCEL
ANIMAL
PROTEIN
COUNT
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

w/
a
WJ
x10­
6/
ti
_______________­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

GROUP:
1474/
840
PPM
MALES
61418
23.9
4.
1.2
61423
25.6
3.
0.7
61428
27.4
3.
1.0
61429
22.0
4.
1.7
61436
17.0
3.
1.2
MEAN
23.2
3.
1.2
S.
D.
3.99
0.5
0.36
N
5
5
5
___________­_____­­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

mg/
dL
=
GRAMS/
DECILITER,
mL
=
MILLILITERS
156
.
.
.
.
.
rnrnrnrnN
157
I.
58
159
TABLE
20
(UNSCHEDULED
mxms)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
PAGE
1
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61451
GROUP
4:
1527
PPM
FEMALE
FOUND
DEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
__________­­________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
KIDNEYS
FINAL
BODY
WT(
G1
123.

LIVER
LARYNX
NASAL
LEVEL
II
NASAL
LEVEL
113
NASAL
LEVEL
IV
NASAL
LEVEL
V
GRADE
_______­__­________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­
MICRO:
NECROSIS,
TUBULAR
1
LUMINAL
DEBRIS
1
SMALL
AMOUNT
OF
BRIGHTLY
EOSINOPHILIC
GRANULAR
MATERIAL
WITHIN
CORTICAL
TUBULE5
THAT
WERE
NOT
NECROTIC.
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
4
BRIDGING
CENTRILOBULAR
NECROSIS
AND
HEMORRHAGE.
MICRO:
INFLAMMATION,
ACUTE
1
ACUTE
TO
SUBACUTE
INFLAMMATION
NEAR
THE
BASE
OF
THE
EPIGLOTTIS
AND
VENTRAL
POUCH.
VOCAL
PROCESSES
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
INFLAMMATION,
SUBACUTE
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
NECROSIS,
SINGLE
CELL
1
NECROSIS
ON
THE
TURBINATES,
PRIMARILY
THE
NASOTURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
3
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MFATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
1
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
20
(UNSCHEDULED
DEATHS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
2
ANIMAL
NO.
61451
GROUP
4:
1527
PPM
FEMALE
FOUND
DEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
CAUSE
DEATH/
MORB
MICRO:
UNDETERMINED
P
SEVERE
HEPATOCELLULAR
NECROSIS
IS
A
POSSIBLE
CAUSE
OF
DEATH,
BUT
BECAUSE
OF
THE
LIMITED
NUMBER
OF
TISSUES
EXAMINED
A
DEFINITIVE
CAUSE
OF
DEATH
WAS
NOT
DETERMINED.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
C&?
WDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVB,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
%­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE.
4­
SEVERE,
P­
PRESENT
PAGE
3
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
20
(UNSCHEDULED
DEATHS)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61462
GROUP
4:
1527
PPM
FEMALE
FOUND
DEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
______
L________­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­*­­­~­­­­­­­

KIDNEYS
FINAL
BODY
WT(
G)
117.

LIVER
LARYNX
MICRO:
LUMINAL
DEBRIS
1
SKIN
SKIN
STOMACH,
GLD
NASAL
LEVEL
II
NASAL
LEVEL
111
NASAL
LEVEL
IV
NASAL
LEVEL
V
SMALL
AMOUNT
OF
BRIGHTLY
EOSINOPHILIC
GRANULAR
MATERIAL
WITHIN
CORTICAL
TUBULES
THAT
WERE
NOT
NECROTIC.
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
BRIDGING
CENTRILOBULAR
NECROSIS
AND
HEMORRHAGE.
MICRO:
INFLAMMATION,
ACUTE
ACUTE
TO
SUSACU!
TE
INFLAMMATION,
VENTRAL
POUCH
AND
DISTAL
LARYNX.
THE
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUTS
WERE
EXAMINED.
GROSS:
RED
MATTING
OCULAR,
RIGHT;
NASAL
GROSS:
CLEAR
MATTING
OCUI.
J+
R,
BILATERRL
MICRO:
ULCERATION
MICRO:
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
4
1
2
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
mm3
20
UJNSCHEDULED
mmw
ACUTE
INH.
TOX.
(WITH
HISTOFATHOLCGYt
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
4
ANIMAL
NO.
61462
GROUP
4:
1527
PPM
FEMALE
FOUND
DEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________­__­____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
NECROSIS
AND
LOSS
OF
THE
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
FACING
TURBINATES
AND
DORAL
SEPTUM.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
CAUSE
DEATH/
MORE
MICRO:
UNDETERMINED
P
SEVERE
HEPATOCELLULAR
NECROSIS
IS
A
POSSIBLE
CAUSE
OF
DEATH,
BUT
BECAUSE
OF
THE
LIMITED
NUMBER
OF
TISSUES
EXAMINED
A
DEFINITIVE
CAUSE
OF
DEATH
WAS
NOT
DETERMINED.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENALMEDULLA
LUNGS
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE.
P­
PRESENT
TABLE
20
(UNSCHEDULED
DEATHS)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
5
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61481
GROUP
4:
1527
PPM
FEMALE
FOUNDDEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­.
_________________­__­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­
LIVER
FINAL
BODY
WT(
G)
113..
LARYNX
STOMACH
STOMACH,
GLD
NASAL
LEVEL
II
NASAL
LEVEL
III
_______­____
1___
11_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
4
MICRO:
INFLAMMATION,
ACUTE
2
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH
AND
VOCAL
PROCESSES.
THE
BASE
OF
THE
EPIGLOTTIS
WAS
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
EDEMA
1
GROSS:
DARK
RED
AREA(
S)
P
ONE,
3
X
1
MM,
GLANDULAR
PORTION
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
CAUSE
DF.
ATH/
MORB
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLGGIC
CORRELATION
FOR
DARK
RED
AREAS.
MICRO:
NECROSIS,
SINGLE
CELL
1
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
3
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
NECROSIS,
SINGLE
CELL
1
CONTINUED
INVOLVEMENT
OF
TRANSITIONAL
EPITHELIUM,
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
4
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
MICRO:
NECROSIS,
OLFACTORY
LPITHELIUM
2
MICRO:
UNDETERMINED
P
SEVERE
HEPATOCELLULAR
NECROSIS
IS
A
POSSIBLE
CAUSE
OF
DEATH,
BUT
BECAUSE
OF
THE
LIMITED
NUMBER
OF
TISSUES
EXAMINED
A
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
mm3
20
(UNSCHEDULED
DExm.
9
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
6
ANIMAL
NO.
61481
GROUP
4:
1527
PPM
FEMALE
FOUND
DEAD
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____­________­_­________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~~­­­­­­­­­­­­­­­­­­­­­­"­­­­­­
DEFINITIVE
CAUSE
OF
DEATH
WAS
NOT
DETERMINED.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
KIDNEYS
LUNGS
STOMACH,
GLD
STOMACH,
NONGLD
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PGRHv4.28
10/
29/
2001
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
1
ANIMAL
NO.
61409
GROUP
1:
0
PPM
MALE
________­­­­­__­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.
(G)
1.67
7.56
1.36
0.90
2.02
0.0388
163.
__­­­­__­_­____­­­­­­­­­­­­
REL.
PHARYNX
1.025
PANCREAS
4.638
0.834
TRACHEA
0.552
NASAL
LEVEL
II
1.239
0.024
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_______­­_­_­_­­­­­_­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
INFLAMMATION,
SUBACUTE
1
MICRO:
ATROPHY,
ACINAR
1
TISSUE
IN
PLANE
WITH
GLANDULAR
STOMACH.
MICRO:
INFILTRATE,
LYMPHOCYTE
1
MICRO:
INFLAMMATION,
SUBACUTE
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.

GRO~
S:
ADRENAL
GLANDS
STERNEBRAE
COLON
DUODENUN
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS
THYMUS
GLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
LARYNX
LUNGS
NASAL
LEVEL
III
NASAL
LEVEL
IV
BRAIN
CECIJM
AORTA
EPIDIDYNIDES
LYMPH
NODE,
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCREAS
PROSTATE
SAL.
GLANDMAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
KIDNEYS
LIVER
STOMACH,
GLD
NASAL
LEVEL
I
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
2
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61410
GROUP
1:

____________­____
­­­­
L­­­­_

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.71
LIVER
7.27
KIDNEYS
1.46
LUNGS
1.03
TESTES
1.70
ADRENAL
GLANDS
0.0370
FINAL
BODY
WI(
G)
173.
REL.

0.988
4.202
0.844
0.595
0.983
0.021
0
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­­______­­­­­­­­­­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

KIDNEYS
MICRO:
NEPHROPATHY
1
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
LARYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAJ.,
MEDULLA
LARYNX
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
~­
MINIMAL,
~­
MILD,
~­
MODERATE,
~­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
3
ANIMAL
NO.
61419
GROUP
1:
0
PPM
MALE
________­__­____­
.­­­­­­­­­­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.66
LIVER
a.
12
KIDNEYS
1.56
LUNGS
0.89
TESTES
1.82
ADRENAL
GLANDS
0.0398
FINAL
BODY
NT(
G)
177.
____
c___­_____­­___­______

REL.
LARYNX
0.938
4.588
0.881
NO
SIGNIFICANT
0.503
CHANGES
OBSERVED
1.028
0.022
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_­_________­____________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
BASE
OF
THE
EPIGLOTTIS
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.

GROSS:
ADRENAL
GLANDS
COLON
ESOPHAGUS
ILEUM
LYMPH
NODE,
MED
LUNGS
RECTUM
SEMINAL
VESICLES
SPLEEN
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
LARYNX
NASAL
LEVEL
I
NASAL
LEVEL
V
STERNEBRAE
BRAIN
CECUM
DUODENUM
AORTA
EPIDIDYMIDES
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
JEJUNM
KIDNEYS
LIVER
NASAL
TISSUE
LARYNX
TESTES
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
PITUITARY
PROSTATE
SAL.
GLANDMAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
STOMACH
THYROID
GLANDS
TRACHEA
ADRENAL
MEDULLA
LUNGS
NASAL
LEVEL
II
NASAL
LEVEL
VI
KIDNEYS
STOMACH,
GLD
NASAL
LEVEL
III
LIVER
TRACHEA
NASAL
LEVEL
IV
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
l,
l,
P­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
4
ANIMAL
NO.
61432
GROUP
1:
0
PPM
MALE
_______________­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
­­
­­­­­__
ABS.
(G)

1.67
7.83
1.57
0.89
2.26
0.0318
191.
,_____­­­­­­­­__­­­­­­­­­­­­
REL.
KIDNEYS
0.874
LIVER
4.099
NASAL
LEVEL
II
0.822
0.466
1.183
0.017
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
.________­­­­­­­­"­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
NEPHROPATHY
1
MICRO:
INFILTRATE,
MONONUCLEAR
1
MICRO:
INFLAMMATION,
SUBACUTE
1
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
NECROSIS,
SINGLE
CELL
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.

GROSS:
ADRENAL
GLANDS
COLON
ESOPHAGUS
ILEUM
LMulpH
NODE,
MED
LUNGS
RECTUM
SEMINAL
VESICLES
SPLEEN
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
STOMACH,
GLD
NASAL
LEVEL
IV
STERNEBRAE
BRAIN
CECUM
DUODENUM
AORTA
EPIDIDYMIDES
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
mJuNuM
KIDNEYS
LIVER
NASAL
TISSUE
LARYNX
TESTES
THYMUSGLAND
NERVE,
SCIATIC
PANCREAS
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
STOMACH
THYROID
GLANDS
TRACHEA
ADRENAL
MEDULLA
TRACHEA
NASAL
LEVEL
V
LARYNX
NASAL
LEVEL
I
NASAL
LEVEL
VI
LUNGS
NASAL
LEVEL
III
GROSS
GRADE
CODE:
l­
SLIGHT,
~­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
5
ANIMAL
NO.
61434
GROUP
1:

_________­­­­_­__
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
NT(
G)
­
­
­
­
­
­
­
_
­
_

ABS.
(G)
1.83
8.84
1.62
1.11
2.20
0.0405
199.
.­__
­_­­
REL.
0.920
4.442
0.814
0.558
1.106
0.020
0
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­­______­­_­_______­__________
II________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECIJM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUSGIAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GIANDMAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
KIDNEYS
LARYNX
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LEVEL
11
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
6
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61396
GROUP
2:
58
PPM
MALE
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
___­___­__
­­­­_­­­­­­­­­­­­­­­­­­­­­
ABS.
(G)
REL.
LARYNX
1.69
0.939
7.12
3.956
1.38
0.767
0.89
0.494
NASAL
LEVEL
II
2.09
1.161
0.0356
0.020
NO
SIGNIFICANT
180.
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
MICRO:
INFLAMMATION,
ACUTE
1
ACUTE
TO
SUBACUTE
INFLAMMATION,
DISTAL
LARYNX.
THE
BASE
OF
THE
EPIGLOTTIS
WAS
NOT
WELL
FRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
INFLAMMATION,
SUBACUTE
1
LATERAL
WALL
OPPOSITE
THE
MAXILLGTURBINATES.

GROSS:
ADREHAL
GLANDS
STERNEBRAE
COLON
DUODENUM
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS
THYMUS
GLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
KIDNEYS
LIVER
TRACHEA
NASAL
LEVEL
III
NASAL
LEVEL
VI
BRAIN
CECUM
AORTA
EPIDIDYMIDES
LYMPH
NODE,
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCRSAS
PROSTATE
SAL.
GLANDMAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
IV
NASAL
LEVEL
V
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HIST~
PATHOL~
GY)
sTuDY
0~
1,1,2­
TCE
IN
RATS
PAGE
7
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61405
GROUP
2:
58
PPM
MALE
­­_­­­­_­­­­­­­­­
­____
­_­__
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.72
LIVER
8.01
KIDNEYS
1.47
LUNGS
0.96
TESTES
1.95
ADRENAL
GLANDS
0.0370
FINAL
BODY
WT(
G)
178.
.­_­­­­­­­­__­­­­_­­­­­­­­­
REL.
LIVER
0.966
LARYNX
4.500
0.826
0.539
NASAL
LEVEL
IV
1.096
0.021
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_­­­___________­_
L__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

MICRO:
INFILTRATE,
MONONUCLEAR
1
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
INFLAMMATION,
SUBACUTE
1
RESPIRATORY
EPITHELIUM.
MICRO:
INFLAMMATION,
SUBACUTE
1
INFLAMMATION,
RESPIRATORY
EPITHELIUM.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
COLON
DUODENUM
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS
THYMUS
GLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAt
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
KIDNEYS
LARYNX
NASAL
LEVEL
II
NASAL
LEVEL
III
BRAIN
CECUM
AORTA
EPIDIDYMIDES
LYMPH
NODE,
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCREAS
PROSTATE
SAL.
GLAND
MAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
V
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
8
ANIMAL
NO.
61413
GROUP
2:
58
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________________________
I_______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
REL.
KIDNEYS
MICRO:
NEPHROPATHY
1
BRAIN
1.67
1.044
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
LIVER
6.62
4.137
LARYNX
MICRO:
INFLAMMATION,
ACUTE
1
KIDNEYS
1.27
0.794
ACUTE
TO
SUBACUTE
INFLAMMATION
AT
OR
NEAR
THE
BASE
OF
THE
LUNGS
0.84
0.525
EPIGLOTTIS
AND
VENTRAL
POUCH.
TESTES
1.79
1.119
ADRENAL
GLANDS
0.0268
0.017
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
FINAL
BODY
WT(
G)
160.
DORSAL
MEATUS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUSGLAND
NERVE.
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLANDMAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CDRD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHSA
URINARY
BLADDER
MICRO:
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
)­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
9
ANIMAL
NO.
61416
GROUP
2:
58
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_________­______________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­~
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.77
LIVER
8.89
KIDNEYS
1.75
LUNGS
1.00
TESTES
2.32
ADRENAL
GLANDS
0.0435
FINAL
BODY
WT.(
G)
197.
REL.
LIVER
0.898
LARYNX
4.513
0.888
0.508
NASAL
LEVEL
II
1.178
0.022
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
MICRO:
INFILTRATE,
MONONUCLEAR
MICRO:
INFLAMMATION,
ACUTE
ACUTE
TO
SUBACUTE
INFLAMMATION,
DISTAL
LARYNX.
EXUDATE,
SUPPURATIVE
MICRO:
INFLAMMATION,
SUBACUTE
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DORSAL
MEATUS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
SINGLE
CELL
NECROSIS,
SEPTUM
AND
ADJACENT
TURBINATE,
UNILATERAL.
MICRO:
INFLAMMATION,
SUBACUTE
INFLAMMATION,
DORSAL
MEATUS.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLANDMAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
MICRO:
KIDNEYS
STOMACH,
GLD
STOMACH,
NONGLD
TRACHEA
NASAL
LEVEL
III
175
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61431
GROUP
2:
58
PPM
­­­­­­­­­­­­­­­­­
­_________
,____­__­__­"_­__­­______^_

ORGAN
WEIGHT
ABS.
(0)
REL.
LARYNX
BRAIN
1.74
1.030
LIVER
7.62
4.509
NASAL
LEVEL
II
KIDNEYS
1.50
0.888
LUNGS
0.95
0.562
TESTES
1.48
0.876
ADRENAL
GLANDS
0.0427
0.025
NO
SIGNIFICANT
FINAL
BODY
WT(
G)
169.
CHANGES
OBSERVED
MALE
PAGE
11
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­­_­­"­___­""""____"­­­­"­­­­­"­­­­­­­­­­­­"­­"­­­­­­"­­­­­­"­­­­­­­­­"­"­­­­­
MICRO:
INFLAMMATION,
ACUTE
1
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH.
MICRO:
METAPLASIA,
SQUAMOUS
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
INFLAMMhTION,
SUBACUTE
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
COLON
DUODENUM
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS
THYMUSGLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
KIDNEYS
LIVER
NASAL
LEVEL
III
NASAL
LEVEL
IV
BRAIN
CECUM
AORTA
EPIDIDYMIDES
LYMPH
NODE,
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCREAS
PROSTATE
SAL.
GLANDMAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2"
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
43.7001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INK.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
12
ANIMAL
NO.
61399
GROUP
3:
181
PPM
MALE
_­­__­­__­­­­­­­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
­L­
­­_­­­

ABS.
(G)
1.74
6.29
1.38
0.64
2.11
0.0399
165.
,­___­______________­­­­­­­­
REL.
KIDNEYS
1.055
LIVER
3.812
LARYNX
0.836
0.509
1.279
NASAL
LEVEL
III
0.024
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
_­­__________­______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
INFLAMMATION,
CHRONIC
MICRO:
INFILTRATE,
MONONUCLEAR
MICRO:
INFLAMMATION,
ACUTE
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH.
EXUDATE,
SUPPURATIVE
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DORSAL
MEATUS,
SEPTUM,
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
SINGLE
CELL
NECROSIS,
PRIMARILY
DORSAL
MEATUS
AND
SEPTUM.

GROSS:
ADRENAL
GLANDS
COLON
ESOPHAGUS
ILEUM
LYMPH
NODE,
MED
LUNGS
RECTUM
SEMINAL
VESICLES
SPLEEN
URINARY
BLADDER
MICRO:
STOMACH,
GLD
STERNEBRAE
BRAIN
CECUM
DUODENUM
AORTA
EPIDIDYMIDES
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
JEJUNUN
KIDNEYS
LIVER
NASAL
TISSUE
LARYNX
TESTES
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
PITUITARY
PROSTATE
SAL.
GLANDMAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
STOMACH
THYROID
GLANDS
TRACHEA
STOMACH,
NONGLD
DAY:
1
GRADE
­
­
_
_
_
_
_
_

1
1
1
NASALLEVEL
II
GROSS
GRADE
CODE:
l­
SLIGHT,
Z­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
FATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
13
ANIMAL
NO.
61411
GROUP
3:
181
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
L
GRADE
_______­_______­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.68
LIVER
6.78
KIDNEYS
1.43
LUNGS
0.88
TESTES
2.02
ADRENAL
GLANDS
0.04rls
FINAL
BODY
WT(
G)
172.
REL.
LIVER
0.977
3.942
0.831
0.512
1.174
LARYNX
0.024
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
MICRO:
NECROSIS
INFILTRATE,
MONONUCLEAR
INFLAMMATION,
SUBACUTE
MONONUCLEAR
CELL
INFILTRATES
TO
SUBACUTE
INFLAMMATION
AROUND
CENTRAL
VEINS.
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
BASE
OF
THE
EPIGLOTTIS
NOT
WELL
PRESENTED
IN
SBCTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DORSAL
MEATIJS,
DORSAL
SEPTUM,
AND
TURBINATES.
GLANDS
SEEMED
TG
BE
SPARED.

GROSS:
ADRENAL
GLANDS
STERNEBFAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDLDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLANDMAND
179
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
15
ANIMAL
NO.
61441
GROUP
3:
181
PPM
MALE
­­_­­­­­­­­­­­­­­­
._­­­­­
­­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.71
LIVER
8.01
KIDNEYS
1.51
LUNGS
0.96
TESTES
2.13
ADRENAL
GLANDS
0.0436
FINAL
BODY
WT(
G)
177.
­­­"­­­­­­­­­­­­­­­­­­"­­­­

REL.
NASAL
LEVEL
III
0.966
4.525
NASAL
LEVEL
IV
0.853
0.542
1.203
NASAL
LEVEL
V
0.025
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
___­____­­___­__­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.

GROSS:
ADRENAL
GLANDS
COLON
ESOPHAGUS
ILEUM
LYMPH
NODE,
MED
LUNGS
RECTUM
SEMINAL
VESICLES
SPLEEN
URINARY
BLADDER
MICRO:
KIDNEYS
STOMACH,
NONGLD
STERNEBRAE
BRAIN
CECUM
DUODENUM
AORTA
EPIDIDYMIDES
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
ER
HEART
JEJIJNUM
KIDNEYS
LIVER
NASAL
TISSUE
LARYNX
TESTES
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
PITUITARY
PROSTATE
SAL.
GLANDMAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
STOMACH
THYROID
GLANDS
TRACHEA
LIVER
NASAL
LEVEL
11
LARYNX
STOMACH,
GLD
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
16
ANIMAL
NO.
61442
GROUP
3:
181
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
______^_____________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.79
LIVER
7.59
KIDNEYS
1.54
LUNGS
0.98
TESTES
2.36
ADRENAL
GLANDS
0.0474
FINAL
BODY
WT(
G)
178.
REL.
LIVER
1.006
4.264
0.865
0.551
IARYNX
1.326
0.027
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
MICRO:
INFLAMMATION,
SUBACUTE
MONONUCLEAR
CELL
INFILTRATES
TO
SUBACUTE
INFLAMMATION
AROUND
CENTRAL
VEINS.
INFILTRATE,
MONONUCLEAR
MICRO:
INFLAMMA
TION,
ACUTE
INFLAMMATION,
FOCAL
FOLDS.
VOCAL
PROCESSES
AND
BASE
OF
THE
EPIGLOTTIS
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUTS
WERE
EXAMINED.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
182
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOFATHOLOGY~
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
18
ANIMAL
NO.
61443
GROUP
3:
181
PPM
MALE
­­­­­_­­­_­­­_­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
­
_­­­­­­­
­­

ABS.
(G)
1.69
7.28
1.42
0.85
1.96
0.0442
167.
.­­­­­­­­­C
­­­­­­­­­­­­­­­­

REL.
KIDNEYS
1.012
LIVER
4.359
0.850
0.509
1.174
LARYNX
0.026
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVELVI
NO
SIGNIFICANT
CHANGES
OBSERVED
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­­___________­__­___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
INFLAMMATION,
CHRONIC
1
MICRO:
INFILTRATE,
MONONUCLEAR
1
INFLAMMATION,
SUBACUTE
1
MOST
OF
THE
INFLAMMATION
IS
SUBCAPSULAR
BUT
SIMILAR
TO
THAT
IN
CENTRILOBULAR
AREAS.
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
MICRO:
NECROSIS,
OLFACTORY
EPITHELXUM
1
GROSS:
ADRENAL
GLANDS
STERNEBRAE
COLON
DUODENUM
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS_
THYMUS
GLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
LARYNX
STOMACH,
GLD
NASAL
LEVEL
III
BRAIN
CECUM
AORTA
EPIDIDYMIDES
LYMPH
NODE.
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCREAS
PROSTATE
SAL.
GLANDMAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
STOMACH,
NONGLD
NASAL
LEVEL
II
GROSS
GRADE
CODE:
l­
SLIGHT,
I­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
I­
MINIMAL.
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PAGE
19
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
[WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
P­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61397
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_________­____
I_____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.72
LIVER
7.37
KIDNEYS
1.56
LUNGS
0.90
TESTES
1.54
ADRENAL
GLANDS
0.0619
FINAL
BODY
NT(
G)
158.
REL.
DUODENUM
1.089
4.665
JEJUNUM
0.987
JEJUNUM
0.570
0.975
0.039
LIVER
STOMACH
STOMACH,
GLD
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
MICRO:
EROSION
PROXIMAL
DUODENUM,
IN
SECTION
WITH
STOMACH.
GROSS:
DARK
RED
CONTENTS
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
DARK
RED
CONTENTS
MAY
HAVE
RESULTED
FROM
THE
GASTRIC
OR
DUODENAL
EROSIONS.
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE.
GROSS:
DARK
RED
CONTENTS
MICRO:
EROSION
EROSIONS
AT
THE
JUNCTION
OF
THE
PYLORIS
AND
DUODENUM
AND
IS
THE
LIKELY
CAUSE
OF
DARK
RED
CONTENTS
IN
THE
GASTROINTESTINAL
TRACT.
MICRO:
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
SINGLE
CELL
CONTINUED
INVOLVEMENT
OF
TRANSITIONAL
EPITHELIUM,
TURBINATES
AND
LATERAL
WALL.
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
PROJECT
NO.:
WIL­
417001
SPONSOR:
H?
iP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
20
ANIMAL
NO.
61397
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­~­~­­­
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
ILEUM
KIDNEYS
LIVER
LYMPH
NODE.
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
THYROID
GLANDS
TF'ACHEA
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
JEJUNUM
KIDNEYS
LARYNX
LUNGS
TRACHEA
NASALLEVELI
GROSS
GRADE
CODE:
1­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
21
ANIMAL
NO.
61406
GROUP
4:
1527
PPM
MALE
­­­­­­­­­­­­_­­­­.
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT.(
G)
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ABS.
(G)
REL.
DUODENUM
1.59
1.046
7.11
4.678
LIVER
1.37
0.901
0.78
0.513
LARYNX
1.86
1.224
0.0469
0.031
152.
STOMACH,
GLD
NASAL
LEVEL
II
.­.­

NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____­______­________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
EROSION
1
PROXIMAL
DUODENUM,
IN
SECTION
WITH
STOMACH.
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOEULAR
3
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE.
MICRO:
INFLFMMATION,
ACUTE
1
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH
AND
VOCAL
PROCESSES.
THE
BASE
OF
THE
EPIGLOTTIS
WAS
NOT
NELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
EROSION
1
MICRO:
NECROSIS,
SINGLE
CELL
2
NECROSIS
ON
THE
TURBINATES,
PRIMARILY
THE
NASOTURBINATES.
MICRO:
NECROSIS,
SINGLE
CELL
2
CONTINUED
INVOLVEMENT
OF
THE
TRANSITIONAL
EPITHELIUM
OF
BOTH
TURBINATES.
NECROSIS,
OLFACTORY
EPITHELIUM
4
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
3
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
THE
EPITHELIUM
AND
UNDERLYING
GLANDS,
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
No.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOL~
Y)
STUDY
0~
1,1,2­
TCB
IN
RATS
PAGE
22
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61406
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____________________­­­­*­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

DORSAL
MEATUS
AND
FACING
TURBINATES
AND
DORbL
SEPTUM.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DORSAL
MEATUS,
DORSAL
SEPTUM,
AND
TURBINATES.
GLANDS
SEEMED
TO
BE
SPARED.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
'GLANDS
STERNE5RAE
COLON
DUODENUM
ESOPHAGUS
EYES/
OPTIC
N.
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
LUNGS
THYMUSGLAND
RECTUM
PITUITARY
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
TRACHEA
NASAL
LEVEL
I
BRAIN
CECUM
AORTA
EPIDIDYMIDES
LYMPH
NODE.
TR/
BR
HEART
KIDNEYS
LIVER
LARYNX
TESTES
NERVE,
SCIATIC
PANCREAS
PROSTATE
SAL.
GLRNDMAND
SKIN
SPINAL
CORD
THYROID
GLANDS
TRACHEA
KIDNEYS
LUNGS
GROSS
GRADE
CODE:
l­
SLIGHT,
Z­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRAnE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
23
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61408
GROUP
4:
1527
PPM
­_­­"_______­__
c_

ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
.__­­­
­
_­­­

ABS.
(0)
1.74
8.37
1.77
1.07
2.13
0.0641
166.
,_­­­­­­­­­­­­­­­­
REL.
ILEUM
1.048
ILEUM
5.042
1.066
JEJUNUM
0.645
JEJUNUM
1.283
0.039
LIVER
LIVER
LARYNX
SKIN
STOMACH
STOMACH,

STOMACH,
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_____________­______­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­
GROSS:
DARK
RED
CONTENTS
P
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
TO
EXPLAIN
DARK
RED
CONTENTS.
GROSS:
DARK
RED
CONTENTS
P
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
TO
EkPLAIN
DARK
RED
CONTENTS.
GROSS:
DARK
RED
AREA(
S)
P
MULTIPLE,
IRREGULARLY
SHAPED,
ALL
LOBES
MICRO:
NECROSIS,
HEPATOCELLULAR,
CBNTRILOBULAR
4
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE
THAT
CORRESPONDS
TO
THE
GROSS
FINDING
OF
DARK
RED
AREAS.
MICRO:
INFLAMMATION,
ACUTE
1
ACUTE
TO
SUBACUTE
INFLAMMATION
OF
THE
VOCAL
PROCESSES,
BASE
OF
THE
EPIGLOTTIS,
AND
VENTRAL
POUCH.
GROSS:
RED
MATTING
P
BUCCAL
GROSS:
DARK
RED
AREA(
S)
P
ONE,
1
MM
IN
DIAMETER,
GLANDULAR;
ONE,
2
MM
IN
DIAMETER,
NONGLANDULAR
GLD
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
FOR
DARK
RED
AREA,
RECUT
SECTION
WAS
EXAMINED.
NONGLD
MICRO:
HEMORRHAGE
3
CORRESPONDS
TO
THE
GROSS
OBSERVATION
OF
DARK
RED
AREAS.
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOZY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
24
ANIMAL
NO.
61408
GROUP
4:
152­
l
PPM
MALE
SCHEDULED
EUTH
02/
26,
'01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____________­­_­__­­____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

NASAL
LEVEL
II
MICRO:
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
NASAL
LEVEL
III
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MSATUS
AND
ETHMOID
TURBINATES.
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE.
TR/
BR
HEART
KIDNEYS
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPINAL
CORD
SPLEEN
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
ILEUM
JEJUNUM
KIDNEYS
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
1­
MINIMAL,
I­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
25
ANIMAL
NO.
61421
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_________­­_____­_­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.67
LIVER
6.30
KIDNEYS
1.44
LUNGS
0.83
TESTES
1.75
ADRENAL
GLANDS
0.0475
FINAL
BODY
WT(
G)
144.
REL.
KIDNEYS
1.160
4.375
LIVER
1.000
0.576
LARYNX
1.215
0.033
SKIN
STOMACH
GROSS:
STOMACH,
OLD
MICRO:

NASAL
LEVEL
II
MICRO:

NASAL
LEVEL
III
MICRO:

NASAL
LEVEL
IV
NASAL
LEVEL
V
MICRO:

MICRO:

MICRO:

MICRO:

MICRO:
LUMINAL
DEBRIS
BASOPHILIC
GRANULAR
MATERIAL
WITHIN
CORTICAL
TuBuLAR
LuMENS.
NECROSIS,
HEPATOCELLULAR,
CENTRILOBIJLAR
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE
NO
SIGNIFICANT
CHANGES
OBSERVED
BASE
OF
THE
EPIGLOTTIS
NOT
WELL
PRESENTED
IN
SECTION,
REClJT
SECTIONS
WERE
EXAMINED.
RED
MATTING
NASAL:
BUCCAL
DARK
RED
CONTENTS
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
TO
EXPLAIN
DARK
RED
CONTENTS.
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERRL
WALL.
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS,
SINGLE
CELL
CONTINUED
INVOLVEMENT
OF
TRANSITIONAL
EPITHELIUM,
TURBINATES
AND
LATERAL
WALL.
NECROSIS,
OLFACTORY
EPITHELfUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
NECROSIS,
OLFACTORY
EPITHELIUM
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
26
ANIMAL
NO.
61421
GROUP
4:
1527
PFM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_____________­__________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
EPIDIDYMIDES
ESOPHAGUS
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
'ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLANDMAND
SEMINAL
VESICLES
SKELETAL
MUSCLE
SPINAL
CORD
SPLEEN
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
LARYNX
LUNGS
STOMACH,
GLD
STOMACH,
NONGLD
TRACHEA
NASALLEVELI
GROSS
GRADE
CODE:
l­
SLIGHT.
Z­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
27
SPoNSoR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61439
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
­__­­­­­­­­­­­­_­
­­­­­­­­­­

ORGAN
WEIGHT
ABS.
(0)
BRAIN
1.78
LIVER
7.90
KIDNEYS
1.80
LUNGS
1.02
TESTES
2.32
ADRENAL
GLANDS
0.0585
FINAL
BODY
NT(
G)
172.
___­________­___­__
REL.
CECUM
1.035
CECUM
4.593
1.047
JEJUNUM
0.593
JEJUNUM
1.349
0.034
KIDNEYS
LIVER
LIVER
LARYNX
STOMACH
STOMACH,
GLD
_____­__________­___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­~­­­~­­­­­­­­­­­­­­­­­­­­
GROSS:
DARK
RED
CONTENTS
P
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CHANGES
TO
EXPLAIN
RED
CONTENTS.
GROSS:
DARK
RED
CONTENTS
P
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
TO
EXPLAIN
DARK
RED
CONTENTS.
MICRO:
MINERALIZATION,
TUBULAR
1
GROSS:
DARK
RED
AREA(
S)
P
SEVERAL,
IRREGULARLY
SHAPED,
ALL
LOBES
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
3
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE
THAT
CORRESPONDS
TO
THE
GROSS
FINDING
OF
DARK
RED
AREAS.
MICRO:
INFLAMMATION,
ACUTE
1
INFLAMMATION,.
WLTH
EXUDATION
AND
ASSOCIATED
PLANT
MATERIAL,
AT
AND
NEAR
THE
BASE
OF
THE
EPIGLOTTIS
AND
DISTAL
LARYNX.
EXUDATE,
SUPPURATIVE
2
VOCAL
PROCESSES
AND
VENTRAL
POUCH
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
GROSS:
DARK
RED
CONTENTS
P
MICRO:
EROSION
1
EROSIONS
AT
THE
JUNCTION
OF
THE
PYLORIS
AND
DUODENUM
AND
IS
THE
LIKELY
CAUSE
OF
DARK
RED
CONTENTS
IN
THE
GASTROINTESTINAL
PAGE
28
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
.RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61439
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________­____________
L__________­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

URINARY
BLADDER
URINARY
BLADDER
NASAL
LEVEL
II
NASAL
LEVEL
III
NASALLEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
TRACT.
ULCERATION
ULCER
AT
THE
PROXIMAL
PYLORIC
REGION
ALSO
A
LIKELY
CAUSE
FOR
DARK
RED
CONTENTS.
GROSS:
DARK
RED
CONTENTS
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
NO
HISTOLOGIC
CORRELATION
TO
EXPLAIN
DARK
RED
CONTENTS.
MICRO:
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
NECROSIS,
RESPIRATORY
EPITHELIUM
NECROSIS,
DORSAL
MEATUS.
MICRO:
NECROSIS.
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MSATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
NOT
EXAMINED
SEVERELY
DAMAGED
PRIOR
TO
TRIMMING,
SECTION
INADEQUATE.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
COLON
DUODENUM
AORTA
EPIDIDYMIDBS
ESOPHAGUS
PROJECT
NO.:
WIL­
417001
SPONSOR:?
lAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1.1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
29
­

ANIMAL
NO.
61439
GROUP
4:
1527
PPM
MALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
"_______________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
EYES/
OPTIC
N.
LYMPH
NODE,
TR/
BR
HEART
ILEUM
KIDNEYS
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
TESTES
LUNGS
THYMUS
GLAND
NERVB,
SCIATIC
PANCREAS
RECTUM
PITUITARY
PROSTATE
SAL.
GLAND
MAND
SEMINAL
VESICLES
SKELBTAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
THYROID
GLANDS
TRACHEA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
CECUM
JEJUNUM
LUNGS
STOMACH,
NONGLD
TRACHEA
URINARY
BLADDER
NASAL
LEVEL
I
NOT
EXAMINED
MICRO:
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61446
GROUP
1:
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
____­_____­______
L__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
REL.
LIVER
MICRO:
INFILTRATE.
MONONUCLEAR
BRAIN
1.69
1.280
NO
SIGNIFICANT
LIVER
5.64
4.273
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
KIDNEYS
1.19
0.902
COLON
LUNGS
0.82
0.621
EYES/
OPTIC
N.
OVARIES
0.0905
0.069
ILEUM
ADRENAL
GLANDS
0.0471
0.036
LYMPH
NODE,
MED
FINAL
BODY
WT(
G)
132.
THYMUS
GLAND
RECTUM
SKIN
THYROID
GLANDS
VAGINA
MICRO:
ADRENAL
CORTEX
LUNGS
NASAL
LEVEL
II
NASAL
LEVEL
VI
STERNEBRAE
BRAIN
CECUM
DUODENUM
AORTA
ESOPHAGUS
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
JEJUNtJM
KIDNEYS
LIVER
NASAL
TISSUE
LARYNX
LUNGS
NERVE,
SCIATIC
OVARIES
PANCREAS
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPINAL
CORD
SPLEEN
STOMACH
TRACHEA
URINARY
BLADDER
UTERUS
PAGE
30
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GFADE
­­__________­_­­_______________________
1
ADRENAL
MEDuLLA
KIDNEYS
LARYNX
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATH~
LCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
31
ANIMAL
NO.
61452
GROUP
ORGAN
WEIGHT
ABS,
(G)
BRAIN
1.63
LIVER
4.98
KIDNEYS
1.11
LUNGS
0.84
OVARIES
0.0816
ADRENAL
GLANDS
0.0462
FINAL
BODY
WT(
G)
130.
1:
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
._______________________________________­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­
REL.
KIDNEYS
MICRO:
INFLAMMATION,
CHRONIC
1
1.254
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
3.831
NO
SIGNIFICANT
0.854
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
0.646
COLON
DUODENUM
AORTA
ESOPHAGUS
0.063
EYES/
OPTIC
N,
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
0.036
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
LARYNX
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LNEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
J­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
I­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
32
ANIMAL
NO.
61453
GROUP
1:

­­­­­_­­­­_­­­­­­
­­­­­­­­­­

ORGAN
WEIGHT
ABS.
(G)

BRAIN
1.57
LIVER
4.46
KIDNEYS
1.09
LUNGS
0.77
OVARfES
0.0867
ADRENAL
GLANDS
0.0461
FINAL
BODY
WT(
G)
114.
.­­­­­­
REL.
1.377
3.912
0.956
0.675
0.076
0.040
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_________­__­_­_­__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­*­­­­­­­­­­­­­­­­­­­­­­­­­­­­
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
LARYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
AND
THE
BASE
OF
THE
EPIGLOTTIS
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
NASAL
LEVEL
II
MICRO:
INFLAMMATION,
SUBACUTE
1
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
KIDNEYS
LARYNX
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
Z­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
B­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
mm3
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
33
ANIMAL
NO.
61456
GROUP
­­­­­__­­­­­_­­­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.
(G)
1.69
4.64
1.16
0.76
0.0721
0.0398
122.
1:
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

REL.
GENERAL
COMMENT
GROSS:
ORGAN
DAMAGED
AT
NECROPSY
P
1.385
BRAIN
3.803
LARYNX
MICRO:
INFLAMMATION,
ACUTE
1
0.951
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH.
0.623
SPLEEN
GROSS:
ACCESSORY
P
0.059
ONE,
LESS
THAN
1
MM
IN
DIAMETER
0.033
SPLEEN
MICRO:
ACCESSORY
SPLEEN
P
CORRESPONDS
TO
THE
GROSS
FINDING
OF
ACCESSORY
SPLEEN.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE.
TR/
ER
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENAL
MEDULLA
KIDNEYS
LIVER
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
~­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
23
(SCHEDULED
NECROFSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
34
ANIMAL
NO.
61491
GROUP
1:
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
_r__­_____­_____­
.­­­­­­­­­_­­­__­­­

ORGAN
WEIGHT
ABS.
(G)
REL.
BRAIN
1.62
1.246
LIVER
5.30
4.077
KIDNEYS
1.14
0.077
LUNGS
0.80
0.615
OVARIES
0.0894
0.069
ADRENAL
GLANDS
0.0486
0.038
FINAL
BODY
WT(
G)
130.
KIDNEYS
LIVER
LARYNX
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
GRADE
__­_____­____­___­______________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

MICRO:
NEPHROPATHY
1
MICRO:
INFILTRATE,
MONONUCLEAR
1
MICRO:
INFLAMMATION,
ACUTE
1
EDEMA
1
ACUTE
TO
SUBACUTE
INFLAMMATION,
DISTAL
LARYNX,
VENTRAZ
FOUCH,
AND
NEAR
THE
BASE
OF
THE
EPIGLOTTIS.
THE
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUTS
WERE
EXAMINED.
EXUDATE,
SUPPURATIVE
1
MICRO:
INFLAMMATION,
SUBACUTE
1
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAt
WALL.
METAPLASIA,
SQUAMOUS
1
MICRO:
INFLAMMATION,
SUBACUTE
1
INFLAMMATION,
MAXILLOTURBINATE.
MICRO:
INFLAMMATION,
SUBACUTE
2
INFLAMMATION
OF
THE
RESPIRATORY
EPITHELIUM,
DORSAL­
LATERAL
MEATUS.
MICRO:
INFLAMMATION,
SUBACUTE
1
INFLAMMATION,
RESPIRATORY
EPITHELIUM.
MICRO:
INFLAMMATION,
SUBACUTE
2
INFLAMMATION,
RESPIRATORY
EPITHELIUM.

GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
COLON
DUODENUM
AORTA
CECUM
ESOPHAGUS
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
Tax.
(WITH
HI~
TOPATHOLGGY)
STUDY
OF
1,1,2­
TIE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
35
ANIMAL
NO.
61491
GROUP
1:
0
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____­______
p__­_________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­

EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SXELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
CORTEX
ADRENALMEDULLA
LUNGS
STOMACH.
GLD
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
ANIMAL
NO.
61467
GROUP
__­­­­­­­­­­­­­_­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.61
LIVER
4.69
KIDNEYS
1.00
LUNGS
0.79
OVARIES
0.0757
TABLE
21
(SCHEDULED
NECROFSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
P­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
36
2:
50
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_____­____­___­­­_______________________­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

REL.
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
1.376
LAKYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
4.009
BASE
OF
THE
EPIGLOTTIS
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
0.855
SECTIONS
WERE
EXAMINED.
0.675
NASAL
LEVEL
II
MICRO:
INFLAMMATION,
SUBACUTE
1
0.065
LATERAL
WALL
OPPOSITE
THE
MAXILLOTUKBINATES.
ADRENAL
GLANDS
0.0377
0.032
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
FINAL
BODY
MT(
G)
117.
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STEKNEBKAE
COLON
DUODENUM
EYES/
OPTIC
N.
OVIDUCTS
ILEUM
JEJUNUM
LYMPH
NODE,
MED
NASAL
TISSUE
THYMUS
GLAND
NERVE,
SCIATIC
RECTUM
PITUITARY
SKIN
SPINAL
CORD
THYKOID
GLANDS
TRACHEA
VAGINA
MICRO:
KIDNEYS
LARYNX
NASAL
LEVEL
III
NASAL
LEVEL
VI
1
1
BRAIN
CECUM
AORTA
ESOPHAGUS
LYMPH
NODE,
TR/
BK
HEART
KIDNEYS
LIVER
LARYNX
LUNGS
OVARIES
PANCREAS
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPLEEN
STOMACH
URINARY
BLADDER
UTERUS
STOMACH,
OLD
STOMACH,
NONGLD
GROSS
GRADE
CODE:
l­
SLIGHT,
Z­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
37
ANIMAL
NO.
61470
GROUP
­____­­"__­­__­­­
­­­­____­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.62
LIVER
4.14
KIDNEYS
1.01
LUNGS
0.71
OVARIES
0.0640
ADRENAL
GLANDS
0.0418
FINAL
BODY
W'?(
G)
119.
2:
58
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
._________________­_____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­*­­­­­­­­­­­­­­
REL.
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
1.361
LARYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
3.479
VOCAL
PROCESSES
AND
THE
BASE
OF
THE
EPIGLOTTIS
WERE
NOT
WELL
0.849
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
0.597
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
0.054
NO
SIGNIFICANT
0.035
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMFH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTBRUS
VAGINA
MICR0:
KIDNEY.
Y
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
38
ANIMAL
NO.
61472
GROUP
_­____________­­­
­­_____­­

ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.67
LIVER
5.27
KIDNEYS
1.21
LUNGS
0.81
OVARIES
0.0892
ADRENAL
GLANDS
0.0496
FINAL
BODY
WT(
G)
131.

l­
o
0
W
2:
58
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
REL.
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
1.275
LARYNX
MICRO:
INFLAMMATION,
ACUTE
1
4.023
ACUTE
To
SUSACUTE
INFLAMMATION,
VENTRAL
POUCH.
THE
BASE
OF
0.924
THE
EPIGLOTTIS
WAS
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
0.618
SECTIONS
WERE
EXAMINED.
0.068
NASAL
LEVEL
II
MICRO:
METAPLASIA,
SQUAMOUS
1
0.038
NASAL
LEVEL
IV
MICRO:
INFLAMMATION,
SUBACUTE
1
INFLAMMATION
OF
THE
RESPIRATORY
EPITHELIUM,
DORSAL­
LATERAL
MBATUS.
NECROSIS,
OLFACTORY
EPITHELIUM
2
DORSAL
MEATUS,
SEPTUM,
AND
ETHMOID
TURBINATES.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
KIDNEYS
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
III
NASAL
LEVEL
V
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
I­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
39
ANIMAL
NO.
61475
GROUP
___­___­­­_­­­_­­­
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.
(G)
1.66
5.13
1.16
0.87
0.0959
0.0571
130.
2:
58
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
._______*~___~__~~~_
r~__~_­_~___~__­___­_____________~_­___
r~~_~~~
r~~­_~~~~~~_____­~_____­_______­_­­­___­
REL.
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
1.277
LARYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
3.346
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
0.892
SECTIONS
WERE
EXAMINED.
0.669
NASAL
LEVEL
III
MICRO:
INFLAMMATION,
SUBACUTE
1
0.074
INFLA~
AT~~
N.
DORSAL
~83~~
s
(RESPIRATORY
EPITHELIUM).
0.044
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHBLIUM
2
DORSAL
MEATUS.
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NASAL
LEVEL
VI
MICRO:
INFLAMMATION,
SUBACUTE
2
INFLAMMATION,
RESPIRATORY
EPITHELIUM.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
KIDNEYS
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
II
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
40
ANIMAL
NO.
61477
GROUP
2:
58
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­~­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.68
LIVER
5.40
KIDNEYS
1.11
LUNGS
0.78
OVARIES
0.0892
ADRENAL
GLAND.
5
0.0444
FINAL
BODY
WT(
G)
127.
REL.
LARYNX
1.323
4.252
0.874
NASAL
LEVEL
II
0.614
0.070
NASAL
LEVEL
III
0.035
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
AND
THE
BASE
OF
THE
EPIGLOTTIS
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
METAPLASIA,
SQUAMOUS
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
INFLAMMATION,
SUBACUTE
RESPIRATORY
EPITHELIUM.
NECROSIS,
OLFACTORY
EPITHELIUM
MICRO:
INFLAMMATION,
SUBACUTE
INFLAMMATION,
RESPIRATORY
EPITHELIUM.
MICRO:
INFLAMMATION,
SUBACUTE
ACUTE
TO
SUBACUTE
INFLAMMATION
OF
THE
TURBINATES
(OLFACTORY
EPITHELIUM).

GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE.
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
NAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
206
PAGE
42
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECR~
PSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61449
GROUP
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
___________________­____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ORGAN
WEIGHT
ABS.
(Cl
BRAIN
1.57
LIVER
5.18
KIDNEYS
1.05
LUNGS
0.69
OVARIES
0.0847
ADRENAL
GLANDS
0.0420
FINAL
BODY
WT(
G)
120.
REL.
LIVER
1.308
4.317
0.875
0.575
0.071
LARYNX
0.035
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
NO
SIGNIFICANT
CHANGES
OBSERVED
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
1
CENTRILOBULAR
NECROSIS
WITH
EXTENSIVE
ASSOCIATED
INFLAMMATION.
INFLAMMATION,
SUBACUTE
1
MONONUCLEAR
CELL
INFILTRATES
TO
SUBACUTE
INFLAMMATION
AROUND
CENTRAL
VEINS.
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
METAPLASIA,
SQUAMOUS
LATERAL
WALL
OPPOSITE
THE
MAXILLOTURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
DORSAL
MEATUS,
SEPTUM,
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPSNAL
CORD
SPLEEN
STOMACH
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
43
ANIMAL
NO.
61449
GROUP
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_______________
I________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­

THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
KIDNEYS
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TILE
21
(SCHEDULED
NEcRoPSY)
ACUTE
INH.
TAX.
(WITH
HIST~
PATHOLOGY)
STUDY
0~
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
44
ANIMAL
NO.
61474
GROUP
3:
181
PPM
FEMALE
SCHEDULED
ELJTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_____________­__________­­__­___­___________­­­­_­__­____­­­_­­­__­___­­­__­­­____­___­_­­___­­­_­­­­­­­______.__________­­_______­_

ORGAN
WEIGHT
ABS.(
G)
REL.
LIVER
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILORULAR
1
BRAIN
1.52
1.357
CENTRILOBULAR
NECROSIS
WITH
EXTENSIVE
ASSOCIATED
INFLAMMATION.
LIVER
4.68
4.179
INFLAMMATION,
SUBACUTE
2
KIDNEYS
1.00
0.893
MONONUCLEAR
CELL
INFILTRATES
TO
SUBACUTE
INFLAMMATION
AROUND
LUNGS
0.69
0.616
CENTRAL
VEINS.
OVARIES
0.0827
0.074
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
ADRENAL
GLANDS
0.0450
0.040
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
FINAL
BODY
WT.(
G)
112.
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
O?
TIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
KIDNEYS
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
II
NASAL
LEVEL
III
GROSS
GRADE
CODE:
l­
SLIGHT,
Z­
MODERATE,
3­
MARKED.
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4SEVERE.
p­
PRESENT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROFSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
45
ANIMAL
NO.
61482
GROUP
_______­­­­­­­­­­
­­
­­­­­­­
ORGAN
WEIGHT
ABS.
(G)
BRAIN
1.63
LIVER
5.44
KIDNEYS
1.12
LUNGS
0.70
OVARIES
0.0685
ADRENAL
GLANDS
0.0455
FINAL
BODY
WT(
G)
126.

E
0
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26,
'01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

REL.
LIVER
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
1
1.294
CENTRILOBLBAR
NECROSIS
WITH
EXTENSIVE
ASSOCIATED
INFLAMMATION.
4.317
INFLAMMATION,
SUBACUTE
2
0.889
MOST
OF
THE
INFLAMMATION
IS
SUBCAPSULAR
BUT
SIMILAR
TO
THAT
IN
0.556
CENTRILOBULAR
AREAS.
0.054
IARYNX
MICRO:
INFLAMMATION,
ACUTE
1
0.036
NASAL
LEVEL
III
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
INFLAMMATION,
SUBACUTE
1
INFLAMMATION,
RESPIRATORY
EPITHELIUM
AND
GLANDS,
SEPTUM.
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
DORSAL
MEATUS,
SEPTUM,
AND
ETHMOID
TURBINATES.
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
WIDELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HE&
RT
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TlSSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
211
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
47
ANIMAL
NO.
61486
GROUP
­­__­­­__­­­­.­­__.
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.(
G)
1.64
5.07
1.12
0.71
0.0816
0.0539
121.
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
.­­___­__­__­__­__­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
REL.
LIVER
MICRO:
INFILTRATE,
MONONUCLEAR
1
1.355
LARYNX
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
4.190
VOCAL
PROCESSES
AND
THE
BASE
OF
THE
EPIGLOTTIS
WERE
NOT
WELL
0.926
FRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
0.587
SKIN
GROSS:
RED
MATTING
P
0.067
OCULAR,
RIGHT
0.045
NASAL
LEVEL
II
MICRO:
NECROSIS,
SINGLE
CELL
1
NECROSIS
ON
THE
TURBINATES,
PRIMARILY
THE
NASOTURBINATES.
EXUDATE
1
NASAL
LEVEL
III
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NASAL
LEVEL
IV
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NASAL
LEVEL
V
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
1
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEERAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPIN&
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
KIDNEYS
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
F­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
PAGE
48
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61493
GROUP
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
___­­­__­­­­­­­___
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WTTG)
­
­
­
­
­
­
­
_
_
ABS.
(G)
1.53
4.76
0.98
0.61
0.0616
0.0441
108.
____­_­_
L___­­_­­___­­­­­

REL.
LIVER
1.417
4.407
LARYNX
0.907
0.565
0.057
NASAL
LEVEL
III
0.041
NASAL
LEVEL
IV
GRADE
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOEIJLAR
2
CENTRILOBm
NECROSIS
WITH
EXTENSIVE
ASSOCIATED
INFLAMMATION.
MICRO:
NO
SIGNIFICANT
CHANGES
OBSERVED
VOCAL
PROCESSES
WERE
NOT
WELL
PRESENTED
IN
SECTION,
RECUT
SECTIONS
WERE
EXAMINED.
MICRO:
INFLAMMATION,
SUBACUTE
1
INFLAMMATION,
NASOTURBINATE.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
THE
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
FACING
TURBINATES
AND
DOFAL
SEPTUM.
NASAL
LEVEL
V
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUS
GLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SKIN
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
PROJECT
NG.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
SNH.
TOX.
(WITH
HISTOPhTHOLOGY)
STUDY
OF
l,
l,
Z­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
49
ANIMAL
NO.
61493
GROUP
3:
181
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
__­__________
I____­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­.­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
MICRO:
KIDNEYS
LARYNX
STOMACH,
GLD
STOMACH,
NONGLD
NASAL
LEVEL
II
NASAL
LEVEL
VI
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
Z­
MILD,
3­
MODERATE,
4­
SEVERE.
P­
PRESENT
ANIMAL
NO.
61476
GROUP
4:
1527
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
___________­­­­­________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.
(G)
1.56
4.33
1.08
0.74
0.0641
0.0513
108.
REL.
DUODENUM
1.444
4.009
1.000
0.685
KIDNEYS
0.059
0.048
LIVER
MICRO:
HYPERPLASIA,
EPITHELIAL
REGENERATIVE
HYPERPLASIA,
WITH
LOSS
OF
VILLI,
IN
THE
PROXIMAL
DUODENUM.
IN
SECTION
WITH
THE
STOMACH.
INFLAMMATION.
ACUTE
2
MICRO:
LUMINAL
DEBRIS
BASOPHILIC
GRANULAR
MATERIAL
WITHIN
CORTICAL
TUBULAR
LUMENS.
GROSS:

MICRO:
PALE
ALL
LOBES
2
cn
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOL~
GY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
50
LIVER
LARYNX
SKIN
GROSS:

SKIN
GROSS:

STOMACH
STOMACH,
GLD
GROSS:
MICRO:
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBUIAR
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION,
AND
HEMORRHAGE
THAT
CORRESPONDS
TO
THE
GROSS
FINDING
OF
PALE.
EDEMA
INFLAMMATION.
ACUTE
ACUTE
TO
SUBACUTE
INFLAMMATION,
VENTRAL
POUCH,
VOCAL
PROCESSES
AND
DISTAL
LARYNX.
THE
BASE
OF
THE
EPIGLOTTIS
WAS
NOT
WELL
PRESENTED
IN
SECTION,
RECUTS
WERE
EXAMINED.
YELLOW
MATTING
UROGENITAL
RED
MATTING
FOREPAW.
BILATERAL;
OCULAR,
BILATERAL;
NASAL;
BUCCAL
DARK
RED
CONTENTS
EROSION
EROSIONS
AT
THE
JUNCTION
OF
THE
PYLORIS
AND
DUODENUM
AND
IS
2
2
PAGE
51
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCXiY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61476
GROUP
4:
1527
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_­__­______­__­­_­­_­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­"­­

STOMACH,
NONGLD
NASAL
LEVEL
II
NASAL
LEVEL
TII
NASAL
LEVEL
IV
NASAL
LEVEL
V
NASAL
LEVEL
VI
NO
SIGNIFICANT
CHANGES
OBSERVED
THE
LIKELY
CAUSE
OF
DARK
RED
CONTENTS
IN
THE
GASTROINTESTINAL
TRACT.
MICRO:
HEMORRHAGE
CORRESPONDS
TO
THE
GROSS
OBSERVATION
OF
DARK
RED
CONTENTS.
MICRO:
NECROSIS,
SINGLE
CELL
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
SINGLE
CELL
CONTINUED
INVOLVEMENT
OF
TRANSITIONAL
EPITHELIUM,
TURBINATES
AND
LATERAL
WALL.
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPXTHELIUM
AND
UNDERLYING
GLANDS.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
NECROSIS
AND
LOSS
OF
THE
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MBATUS
AND
FACING
TURBINATES
AND
DORAL
SEPTUM.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
GROSS:
ADRENAL
GLANDS
STERNEBRAB
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
S!?
ONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
52
ANIMAL
NO.
61476
GROUP
4:
1527
PPM
FEMALE
SCHEDULED
EUTH
­_­­­­­
1­__­­­­___­­­.

EYES/
OPTIC
N.
ILEUM
NASAL
TISSUE
NERVE,
SCIATIC
PITUITARY
SPLEEN
UTERUS
MICRO:
ADRENAL
CORTEX
NASAL
LEVEL
I
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
GRADE
_­­_____­_______­_­_____________________­­­­­­­­­­­­­­­­­
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
JEJUNUN
KIDNEYS
LYMPH
NODE,
MED
LARYNX
LUNGS
THYMUS
GLAND
OVARIES
PANCREAS
RECTUM
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPINAL
CORD
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
VAGINA
ADRENAL
MEDULLA
LUNGS
TRACHEA
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
4­
SEVERE,
P­
PRESENT
TABLE
21
(SCHEDULED
NECROPSY)
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
53
SPONSOR:
HAP
TASK
FORCE
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
ANIMAL
NO.
61484
GROUP
4:
1527
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
1
­­­­­_­­­­­­­_­.
ORGAN
WEIGHT
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
ADRENAL
GLANDS
FINAL
BODY
WT(
G)
ABS.
(G)
1.63
4.63
1.12
0.67
0.0729
0.0584
103.
_____­_­_­­­­­­­­­­_­­­­­­
REL.
ADRENAL
CORTEX
1.583
LIVER
4.495
1.087
LARYNX
0.650
0.071
0.057
SKIN
NASAL
LEVEL
II
NASAL
LEVEL
III
NASAL
LEVEL
IV
NASAL
LEVEL
V
GRADE
._­­______­_____________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­

MICRO:
NECROSIS
1
MICRO:
NECROSIS,
HEPATOCELLULAR,
CENTRILOBULAR
3
BRIDGING
CENTRILOBULAR
NECROSIS,
INFLAMMATION.
AND
HEMORRHAGE.
MICRO:
INFLAMMATION.
ACUTE
2
ACUTE
TO
SUBACUTE
INFLAMMATION
OF
THE
VOCAL
PROCESSES,
BASE
OF
THE
EPIGLOTTIS,
AND
VENTRAL
POUCH.
GROSS:
RED
MATTING
P
OCULAR,
BILATERAL;
BUCCAL
MICRO:
NECROSIS,
SINGLE
CELL
2
TRANSITIONAL
EPITHELIUM
OF
TURBINATES
AND
LnTERAL
WALL.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
4
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS.
NECROSIS,
SINGLE
CELL
3
CONTINUED
INVOLVEMENT
OF
TRANSITIONAL
EPITHELIUM,
TURBINATES
AND
LATERAL
WALL.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
3
NECROSIS
AND
LOSS
OF
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
ETHMOID
TURBINATES.
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
NECROSIS
AND
LOSS
OF
THE
EPITHELIUM
AND
UNDERLYING
GLANDS,
DORSAL
MEATUS
AND
FACING
TURBINATES
AND
DORAL
SEPTUM.
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
21
(SCHEDULED
NECROPSY)
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
GROSS
AND
MICROSCOPIC
DESCRIPTION
OF
ORGANS
PAGE
54
ANIMAL
NO.
61484
GROUP
4:
1527
PPM
FEMALE
SCHEDULED
EUTH
02/
26/
01
DATE
OF
DEATH:
02/
26/
01
STUDY
DAY:
3
GRADE
_­_
I_________­_________________
I________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

NASAL
LEVEL
VI
MICRO:
NECROSIS,
OLFACTORY
EPITHELIUM
2
DIFFUSELY
SCATTERED
SINGLE
CELL
NECROSIS.
NO
SIGNIFICANT
CHANGES
OBSERVED
GROSS:
ADRENAL
GLANDS
STERNEBRAE
BRAIN
CECUM
COLON
DUODENUM
AORTA
ESOPHAGUS
EYES/
OPTIC
N.
OVIDUCTS
LYMPH
NODE,
TR/
BR
HEART
ILEUM
JEJUNUM
KIDNEYS
LIVER
LYMPH
NODE,
MED
NASAL
TISSUE
LARYNX
LUNGS
THYMUSGLAND
NERVE,
SCIATIC
OVARIES
PANCREAS
RECTUM
PITUITARY
SAL.
GLAND
MAND
SKELETAL
MUSCLE
SPINAL
CORD
SPLEEN
STOMACH
THYROID
GLANDS
TRACHEA
URINARY
BLADDER
UTERUS
VAGINA
MICRO:
ADRENAL
MEDULLA
KIDNEYS
LUNGS
STOMACH,
GLD
TRACHEA
NASAL
LEVEL
I
GROSS
GRADE
CODE:
l­
SLIGHT,
2­
MODERATE,
3­
MARKED,
P­
PRESENT
MICRO
GRADE
CODE:
l­
MINIMAL,
2­
MILD,
3­
MODERATE,
I­
SEVERE,
P­
PRESENT
PGRHv4.28
10/
29/
2001
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
22
ACUTE
INH.
TOX.
(WITH
HI~
TOPATHOL~
GY)
STUDY
0~
1,1,2­
TIE
IN
RATS
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
PAGE
1
DAY
1
MALE
GROUP:
0
PPM
__
L____________­________________________­­­­­­­­­­­­­­­­.­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FEW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
__________________________­______­_­­­­__­­___­­____­­_________­­­­­­­­­­_­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­~~~­~~­­~­­­­­­­

61409
163.
1.67
7.56
1.36
0.90
2.02
0.0388
61410
173.
1.71
7.27
1.46
1.03
1.70
0.0370
61419
177.
1.66
8.12
1.56
0.89
1.82
0.0398
61432
191.
1.67
7.83
1.57
0.89
2.26
0.0318
61434
199.
1.83
8.84
1.62
1.11
2.20
0.0405
MEAN
181.
1.71
7.92
1.51
0.96
2.00
0.0376
S.
D.
14.4
0.071
0.601
0.104
0.101
0.240
0.00349
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
PROJECT
NO.
:w1L­
417001
SPQNSDR:
HAP
TASK
FORCE
TABLE
22
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGYI
STUDY
OF
1,1,2­
TCE
IN
RATS
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
PAGE
2
DAY
1
MALE
GROUP:
58
PPM
________________­_______________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FEW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
_______________­____­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61396
180.
1.69
7.12
1.38
0.89
2.09
0.0356
61405
178.
1.72
8.01
1.47
0.96
1.95
0.0370
61413
160.
1.67
6.62
1.27
0.84
1.79
0.0268
61416
197.
1.77
8.89
1.75
1.00
2.32
0.0435
61431
169.
1.74
7.62
1.50
0.95
1.48
0.0427
MEAN
177.
1.72
7.65
1.47
0.93
1.93
0.0371
S.
D.
13.8
0.040
0.867
0.178
0.063
0.316
0.00672
N
5
5
5
5
5
5
5
FEW
=
FINAL
BODY
WEIGHT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
22
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1.1,2­
TCE
IN
RATS
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
PAGE
3
DAY
1
MALE
GROUP:
181
PPM
________________________________________­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­*­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
____
E______
s__­__­______________________­­­­­­­­­­­­­­­­~~~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­~

61399
165.
1.74
6.29
1.38
0.84
2.11
0.0399
61411
172.
1.68
6.70
1.43
0.88
2.02
0.0405
61441
177.
1.71
8.01
1.51
0.96
2.13
0.0436
61442
178.
1.79
7.59
1.54
0.98
2.36
0.0474
61443
167.
1.69
7.20
1.42
0.85
1.96
0.0442
MEAN
172.
1.72
7.19
1.46
0.90
2.12
0.0431
S.
D.
5.8
0.044
0.674
0.067
0.064
0.153
0.00304
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
22
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
l.,
1,2­
TCE
IN
RATS
PAGE
4
SPONSOR:
UAP
TASK
FORCE
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
DAY
1
MALE
GROUP:
1527
PPM
____________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­

ANIMAL
FBW(
G)
­­­­­­­­­­­­­­­­­­­­­­­­­­

61397
158.
61406
152.
61408
166.
61421
144.
61439
172.
BRAIN
__"_____­_­___
1.72
1.59
1.74
1.67
'1.78
­­­­­
ADRENAL
GLANDS
LIVER
KIDNEYS
LUNGS
TESTES
­­­­­­­­­­­­­­­­­
___­______­­_­_­______________________
c_­­­­­­­­­­­­­­~­­
7.37
1.56
0.90
1.54
7.11
1.37
0.78
1.86
8.37
1.77
1.07
2.13
6.30
1.44
0.83
1.75
7.90
1.80
1.02
2.32
0.0619
0.0469
0.0641
0.0475
0.0585
MEAN
158.
1.70
7.41
1.59
0.92
1.92
0.0558
S.
D.
11.1
0.073
0.788
0.193
0.123
0.309
0.00809
N
5
5
5
5
5
5
5
FBW
5
FINAL
BODY
WEIGHT
TABLE
22
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SPONSOR:
HAP
TASK
FORCE
PAGE
5
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
DAY
1
FEMALE
GROUP:
0
PPM
__________
sL_____________________­­__­­____­­__­__­______________­­­­­­­____________­­__­__________­_______­­­­­­~~­­­­­­­­­­­­­­­­.

ANIMAL
FBW(
G)
BRAIN
ADRENAL
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
__­_____­_____­___­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­"­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61446
132.
1.69
5.64
1.19
0.82
61452
130.
0.0905
1.63
0.0471
4.98
1.11
0.84
61453
114.
0.0816
1.57
0.0462
4.46
1.09
0.77
61456
122.
0.0867
1.69
0.0461
4.64
1.16
0.76
61491
130.
0.0721
1.62
0.0398
5.30
1.14
0.80
0.0894
0.0488
MEAN
126.
1.64
5.00
1.14
S.
D.
0.80
7.5
0.0841
0.051
0.480
0.0456
0.040
0.033
N
0.00752
5
5
5
5
0.00342
5
5
5
FBW
=
FINAL
BODY
WEIGHT
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
22
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)

FEMALE
GROUP:
SE
Pm
PAGE
6
DAY
1
ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
"__­______­­___­__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61467
117.
1.61
4.69
1.00
0.79
0.0757
0.0377
61470
119.
1.62
4.14
1.01
0.71
0.0640
O.
OGlEl
61472
131.
1.67
5.27
1.21
0.81
0
*
0892
0.0496
61475
130.
1.66
5.13
1.16
0.87
0.0959
0.0571
61477
127.
1.68
5.40
1.11
0.78
0.0892
0.0444
MEAN
125.
1.65
4.93
1.10
0.79
0.0828
0.0461
S.
D.
6.4
0.031
0.514
0.092
0.058
0.01282
0.00750
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
K
cn
PROJECT
NO.:
WIL­
417001
SPONSOR:
HAP
TASK
FORCE
TABLE
22
ACUTE
INH.
TAX.
(WITH
HISTOPATHOLOGY)
STUDY
0~
1,1,2­
TCE
IN
RATS
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
PAGE
7
DAY
1
FEMALE
GROUP:
181
PPM
_____­__________________________________­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
___________­_____­______________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­~

61449
120.
1.57
5.18
1.05
0.69
0.0847
0.0420
61474
112.
1.52
4.68
1.00
0.69
0.0827
0.0450
61482
126.
1.63
5.44
1.12
0.70
0.0685
0.0455
61486
121.
1.64
5.07
1.12
0.71
0.0816
0.0539
61493
108.
1.53
4.76
0.98
0.61
0.0616
0.0441
MEAN
117.
1.58
5.03
1.05
0.68
0.0758
0.0461
S.
D.
7.3
0.055
0.311
0.065
0.040
0.01019
0.00456
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
22
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLCGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
0
SEQNSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
AND
FINAL
BODY
WEIGHTS
(GRAMS)
DAY
1
FEMALE
GROUP:
1527
PPM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­
ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLRNDS
___________________________________^____­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61476
108.
1.56
4.33
1.08
0.74
0.0641
0.0513
61484
103.
1.63
4.63
1.12
0.67
0.0729
0.0584
MEAN
106.
1.60
4.48
1.10
0.71
0.0685
0.0549
S.
D.
3.5
0.049
0.212
0.028
0.049
0.00622
0.00502
N
2
2
2
2
2
2
2
FBW
=
FINAL
BODY
WEIGHT
POFBWv4.05
06/
12/
2001
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
0~
1,1,2­
TCE
IN
RATS
PAGE
1
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
MALE
GROUP:
0
PPM
_______________________________________
1­­­­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­~~­~­­­­­­­.­­­~­­­­~~­­­­­­­­­­­­~­~­­­­­­­­­­

ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­

61409
163.
1.025
4.638
0.834
0.552
1.239
0.024
61410
173.
0.988
4.202
0.844
0.595
0.983
0.021
61419
171.
0.938
4.588
0.881
0.503
1.028
0.022
61432
191.
0.874
4.099
0.822
0.466
1.183
0.017
61434
199.
0.920
4.442
0.814
0.558
1.106
0.020
MEAN
181.
0.949
4.394
0.839
0.535
1.108
0.021
S.
D.
14.4
0.0589
0.2363
0.0261
0.0505
0.1058
0.0026
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATH~
L~
GY)
STUDY
OF
1,1.2­
TCE
IN
RATS
PAGE
2
SP0NSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
MALE
GROUP:
58
PPM
___­__­­_­­­­­­
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
________________________________________­____________­­_____.._________________­.________
LUNGs____________­~~~~~~­­­­­­­­­­­­­~~~~­
61396
180.
0.939
3.956
0.767
0.494
1.161
0.020
61405
178.
0.966
4.500
0.826
0.539
1.096
0.021
61413
160.
1.044
4.138
0.794
0.525
1.119
0.017
61416
197.
0.898
4.513
0.888
0.508
1.178
0.022
61431
169.
1.030
4.509
0.868
0.562
0.876
0.025
MEAN
177.
0.975
4.323
0.833
0.526
1.086
0.021
S.
D.
13.8
0.0614
0.2603
0.0547
0.0265
0.1218
0.0029
N
5
5
­5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
23
PROJECT
NO.:
WIL­
417'001
ACUTE
INH.
TOX.
(NITH
HXSTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
3
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
MALE
GROUP:
181
PPM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61399
165.
1.055
3.812
0.836
0.509
1.279
0.024
61411
172.
0.977
3.942
0.831
0.512
1.174
0.024
61441
177.
0.966
4.525
0.853
0.542
1.203
0.025
61442
178.
1.006
4.264
0.865
0.551
1.326
0.027
61443
167.
1.012
4.359
0.850
0.509
1.174
0.026
MEAN
172.
1.003
4.180
0.847
0.525
1.231
0.025
S.
D.
5.8
0.0348
0.2959
0.0137
0.0203
0.0682
0.0013
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
4
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
MALE
GROUP:
1527
PFM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ADRENAL
ANIMAL
FBW(
GJ
BRAIN
LIVER
KIDNEYS
LUNGS
TESTES
GLANDS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61397
158.
1.099
4.665
0.987
0.570
0.975
0.039
61406
152.
1.046
4.678
0.901
0.513
1.224
0.031
61408
166.
1.048
5.042
1.066
0.645
1.283
0.039
61421
144.
1.160
4.37s
1.000
0.576
1.215
0.033
61439
172.
1.035
4.593
1.047
0.593
1.349
0.034
MEAN
158.
1.076
4.671
1.000
0.579
1.209
0.035
S.
D.
11.1
0.0515
0.2405
0.0643
0.0474
0.1415
0.0036
N
S
S
S
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INK.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
5
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
FEMALE
GROUP:
0
PPM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61446
132.
1.280
4.273
0.902
0.621
0.069
0.036
61452
130.
1.254
3.831
0.854
0.646
0.063
0.036
61453
114.
1.377
3.912
0.956
0.675
0.076
0.040
61456
122.
1.385
3.803
0.951
0.623
0.059
0.033
61491
130.
1.246
4.077
0.877
0.615
0.069
0.038
MEAN
126.
1.308
3.979
0.908
0.636
0.067
0.037
S.
D.
7.5
0.0675
0.1958
0.0449
0.0248
0.0065
0.0026
N
5
5
5
5
5
5
5
FBW
=
FINAL
BODY
WEIGHT
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLGGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
6
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
FEMALE
GROUP:
58
PPM
________________________________________­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­~­­­~­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­~

ANIMAL
FEW(
G)
BRAIN
LIVER
___________________­­­­­­­­­
._­__________­______
._­­__­­_
­

61467
117.
1.376
4.009
61470
119.
1.361
3.479
61472
131.
1.275
4.023
61475
130.
1.277
3.946
61477
127.
1.323
4.252
KIDNEYS
­­­__­­­__­"­­­
0.855
0.849
0.924
0.892
0.874
­­,
LUNGS
­­­­­­­­­­­­­__­_­­­­­­
0.675
0.597
0.618
0.669
0.614
OVARIES
_­­­_­­­­­­­­­
_
­­_­
­_
0.065
0.054
0.068
0.074
0.070
ADRENAL
GLANDS
­­­­­­­­­
0.032
0.035
0.038
0.044
0.035
MEAN
125.
S.
D.
6.4
N
5
FBW
=
FINAL
BODY
WEIGHT
1.322
3.942
0.879
0.635
0.066
0.037
0.0466
0.2835
0.0304
0.0351
0.0076
0.0045
5
5
5
5
5
5
TABLE
23
PROJECT
NO.:
WIL­
417001
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
PAGE
7
SPONSOR:
HAP
TASK
FORCE
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
DAY
1
FEMALE
GROUP:
161
PPM
________________________________________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

ADRENAL
ANIMAL
FBWtG)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
____________________­­­­­­­­­­­­­­­­­­­­­­­"­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

61449
120.
1.308
4.317
0.875
0.575
0.071
0.035
61474
112.
1.357
4.179
0.893
0.616
0.074
0.040
61482
126.
1.294
4.317
0.889
0.556
0.054
0.036
61486
121.
1.355
4.190
0.926
0.587
0.067
0.045
61493
108.
1.417
4.407
0.907
0.565
0.057
0.041
MEAN
117.
1.346
4.282
0.898
0.580
0.065
0.039
S.
D.
7.3
0.0485
0.0964
0.0194
0.0233
0.0087
0.0040
N
5
5
5
5
5
5
5
FEW
=
FINAL
BODY
WEIGHT
PROJECT
NO.:
WIL­
417001
Sl=
'ONSOR:
:HAP
TASK
FORCE
TABLE
23
ACUTE
INH.
TOX.
(WITH
HIST~
PATH~
LOGY)
STUDY
OF
1,1,2­
Tc~
IN
RATS
ORGAN
WEIGHTS
RELATIVE
TO
FINAL
BODY
WEIGHTS
(GRAMS/
100
GRAMS)
PAGE
8
DAY
1
FEMALE
GROUP:
1527
PPM
___­____­­__________­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­.­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ADRENAL
ANIMAL
FBW(
G)
BRAIN
LIVER
KIDNEYS
LUNGS
OVARIES
GLANDS
_________________­__­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
61476
108
*
1.444
4.009
1.000
0.685
0.059
0.048
61484
103.
1.583
4.495
1.087
0.650
0.071
0.057
MEAN
106.
1.514
4.252
1.044
0.668
0.065
0.053
S.
D.
3.5
0.0983
0.3437
0.0615
0.0247
0.0085
0.0064
N
2
2
2
2
2
2
2
FBW
=
FINAL
BODY
WEIGHT
POFBWv4.05
06/
12/
2001
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,1.2­
Trichloroethane
(1,1.2­
TCE)
in
Rats
APPENDIX
A
Certificate
of
Analvsis
(Sponsor­
Provided
Data)

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Chemical
Company
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icir
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of
Analysis
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0.
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NOKC:
66433131
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(as
HC:)
YB"
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1
50
Non­
volacils
Rttsiduc
Pm
1
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m
2u
237
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1.1.2­
Trichloroethane
(1.1.2­
TCE)
in
Rats
APPENDIX
B
Serological
Screeniw
(BioReliance
Cornoration)

­238­
Quality
Assurance
Statement
Study
Title:
RAT
LEVEL
II
STUDY
Study
Number:
MVZ172.
WIL
Principal
Investigator:
Kathleen
M.
Vincent
This
study
has
been
divided
into
a
series
of
in­
process
phases.
Using
a
random
sampling
approach,
Quality
Assurance
monitors
each
of
these
phases
over
a
series
of
studies.
Procedures.
documentation,
equipment
records,
etc..
are
examined
in
order
to
assure
that
the
study
is
performed
in
accordance
with
the
U.
S.
FDA
Good
Laboratory
Practice
Regulations
(21
CFR
SS),
the
U.
S.
EPA
GLPs
(40
CFR
792
and
40
CFR
160).
the
UK
GLP
Regulations,
the
Japanese
GLP
Standard,
and
the
OECD
Principles
of
Good
Laboratory
Practice
and
to
assure
that
the
study
is
conducted
according
to
the
protocol
and
reievant
Standard
Operating
Procedures.

The
following
are
the
inspection
dates,
phases
inspected,
and
report
dates
of
QA
inspections
of
this
study.

Inspect
On
23­
Feb­
01
­
23­
Feb­
01
To
Prin
Invst
23­
Feb­
01
To
Mgmt
02­
Mar­
01
Phase
Addition
of
conjugate
to
wells
inspect
On
Phase
2%
Feb­
01
­
2%
Feb­
0
1
To
Prin
Invst
0
1
­Mar­
O1
To
Mgmt
02­
Mar­
01
Final
Report
This
report
describes
the
methods
and
procedures
used
in
the
study
and
the
reported
results
accurately
reflect
the
raw
data
of
the
study.

EIahe
Siadatpour,
B.
S.
QUALITY
ASSURANCE
Hbc&
2
At­
A
DATE
Page
3
241
wL417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
I,
1,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
APPENDIX
C
Pretest
Clinical
Observations
­242­
243
PAGE
2
PROJECT
NO.:
WIL­
417001P
SPONSOR:
HAP
TASK
FORCE
ACUTE
INH.
TOX.
(WITH
HISTOPATHOLOGY)
STUDY
OF
1,1,2­
TCE
IN
RATS
SUMMARY
OF
CLINICAL
FINDINGS:
TOTAL
OCCURRENCE/
NO.
OF
ANIMALS
___­_
F
E
i%,
A
L
E
_­___

NORMAL
­NO
SIGNIFICANT
CLINICAL
OBSERVATIONS
91/
49
DISPOSITION
­SENT
TO
NECROPSY
FOR
SCHEDULED
PATHOLOGY
EVALUATION
­ASSIGNED
TO
WIL­
417001
5/
5
40/
40
BODY/
INTEGUMENT
­SCABBING
DORSAL
HEAD
11
1
EYES/
EARS/
NOSE
­ABSENCE
OF
RIGHT
EYE
11
1
­DRIED
RED
MATERIAL
AROUND
LEFT
EYE
11
1
­DRIED
RED
MATERIAL
AROUND
RIGHT
EYE
1/
1
­b
­HAIR
LOSS
AROUND
RIGHT
EYE
11
1
­­­­­­­­­­_­­_­­­­­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­~­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
l­
PRETEST
PCSUV4.0
06/
l.
3/
2001
WIL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,1,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
APPENDIX
D
Clinical
Pathology
Methods,
Procedures
and
References
­245­
wIL­
417001
Hazardous
Air
Pollutant
Task
Force
CLINICAL
PATHOLOGY
METHODS,
PROCEDURES
AND
REFERENCES
Serum
Chemistry
­
Hitachi
911
Total
Protein
­
Endpoint
biuret
method
that
utilizes
a
sample
blank.
Hitachi
911
Application
Code
166.
Roche
Reagent,
catalog
number
1040901.

Total
White
Blood
Cell
Count
Mix
equal
volumes
of
cell
suspension
and
0.4%
neutral
red
stain
(prepared
in
0.9%
NaCl)
(for
example,
use
100
p.
L
of
each).
Cap
tube
and
incubate
at
approximately
37°
C
for
IO­
15
minutes.

Perform
hemacytometer
count
(normally
10
squares,
5
squares
on
each
side,
are
counted).
Calculate
the
number
of
cells
per
ti
Average
no.
of
cells
per
square
x
lo4
x
2
(2
is
for
staining
dilution).

246
WIL­
41700
1
Hazardous
Air
PoIlutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
Studv
of
1,1,2­
Trichloroethane
(1
,I
.2­
TCE)
in
Rats
APPENDIX
E
Bronchoalveolar
Lavage
Cytology
Report
iGail
L.
Walter,
D.
V.
M.,
D.
A.
C.
V.
P..
D.
A.
B.
T.,
Clinical
Pathology
Consultant)

The
following
points
should
be
noted
for
Appendix
E:

1.
Due
to
the
unscheduled
death
of
one
Group
4
female,
there
were
not
always
five
animals/
sex/
group
evaluated.
Therefore,
on
page
1
of
this
cytology
report,
the
sixth
sentence
of
the
first
paragraph
does
not
accurately
reflect
the
number
of
animals
evaluated
for
Group
4..

2.
Although
it
cannot
be
confirmed
at
this
time,
it
appears
that
for
male
no.
61429,
a
total
of
201
cells
were
identified.
Therefore,
on
page
1
of
this
cytology
report,
the
final
sentence
of
the
first
paragraph
may
not
be
correct
for
this
animal.

These
items
listed
above
did
not
adversely
affect
the
interpretation
of
the
results
for
this
study.

­247­
Clinical
Pathology
Report
M/
IL
Study
Number:
417001
Bronchoalveolar
Lavage
Cytology
Title:
Acute
inhalation
Toxicity
(with
Histopathology)
Study
of
1,1,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
Introduction
This
study
was
performed
at
WIL
Research
Laboratories,
Inc.,
1407
George
Road,
Ashland.
OH
448059281.
The
objective
of
this
study
was
to
characterize
the
toxicologic
response
(including
the
exposure
levei­
response
relationships)
of
the
rat
following
a
single
acute
inhalation
exposure
by
evaluating
gross
pathology
and
histopathology,
as
well
as
effects
of
body
weight,
clinical
condition
and
mortality.
To
characterize
potential
adverse
effects
on
the
respiratory
tract,
bktopathological
examination
of
fixed
tissues
and
evaluation
of
the
cellular
and
biochemicai
parameters
in
bronchoaiveolar
lavage
fluid
(BALF)
was
performed.
This
report
addresses
evaluation
of
the
cehular
component
of
BALF.
Fischer
344
CDF
@(
F­
344)
Kri:
BR
rats
were
exposed
to
0,
50,200
or
1500
ppm
of
I
,I
,2­
TCE
for
4
hours.
Approximately
24
hours
following
completion
of
the
exposure,
bronchoalveolar
kvage
was
performed
and
BALF
was
collected
from
5
ratskex/
group.
Total
cell
counts
were
performed
and
an
aliquot
was
used
for
preparation
of
slides
for
difkrential
cell
counts.
Differential
counts
were
performed
by
Dr.
Gail
L.
Walter
by
visual
identification
of
200
nucleated
cells
peranimal.

Bronchoalveolar
Lavage
Cytology
The
BALF
slides
were
well
prepared
and
adequate
for
evaluation
There
were
no
alterations
in
BALF
total
cell
counts
or
difkentiai
cell
counts
that
were
considered
tu
be
due
to
test
article
exposure.
The
majority
of
cells
present
were
large
mononuclear
cells,
with
a
small
number
of
Lymphocytes
also
present
in
most
samples.
One
­
2
neutrophils
were
present
in
4
of
the
rats
in
the
1500
ppm
group.
However,
one
neutrophil
was
also
noted
in
one
female
tiom
the
control
and
50
ppm
groups.
therefore
these
low
numbers
of
neutrophils
in
the
high
dose
group
were
not
clearly
related
to
test
article
adminktration
A
large
number
of
red
blood
WIL­
417001
I
248
cells
(RBC)
was
present
in
one
male
in
dosed
at
1500
ppm.
Because
RBC's
were
not
seen
in
large
numbers
in
any
other
samples.
this
was
considered
to
be
incidental
and
not
clearly
related
to
test
article
administration.

Gd
L.
Walter,
DVM
Diplomate,
American
College
of
Veterinary
Pathologists
Diplomate.
American
Board
of
Toxicology
Clinical
Pathology
Consultant
Date
W­
IL­
417001
249
2
250
251
Appendix
D
(Table
1)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,I
,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Bronchoalveolar
Lavage
Fluid
Leukocyte
Differential
Count
(%)
­
Summary
of
Means
MALES
Group
No.
Large
Mononuclear
Cells
(%)
Lymphocytes
Neutrophils
(%)
%
Eosinophils
(%)
Epithelial
Cells
(%)
Mitotic
Figures
(%)

1
MEAN
98.8
0.8
0.0
0.0
0.4
0.0
SD
1.15
0.57
0.00
0.00
0.89
0.00
N
5
5
5
5
5
5
2
MEAN
97.0
3.0
0.0
0.0
0.0
0.0
SD
5.2
5.2
0.0
0.0
0.0
0.0
N
3
3
3
3
3
3
3
MEAN
98.6
0.9
0.0
0.0
0.4
0.1
SD
1.11
0.85
0.00
0.00
0.48
0.25
N
4
4
4
4
4
4
4
MEAN
97.5
0.6
0.4
0.1
1.1
0.3
SD
2.03
0.65
0.55
0.22
1.75
0.67
N
5
5
5
5
5
5
1
Appendix
D
(Table
1)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,l
,PTrichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Bronchoalveolar
Lavage
Fluid
Leukocyte
Differential
Count
(%)
­
Summary
of
Means
FEMALES
Group
No.
Large
Mononuclear
Cells
(%)
Lymphocytes
Neutrophils
(%)
(%)
Eosinophils
(%)
Epithelial
Cells
(?/)
Mitotic
Figures
(%)

1
MEAN
99.0
0.9
0.1
0.0
0.0
0.0
SD
0.94
0.74
0.22
0.00
0.00
0.00
N
5
5
5
5
5
5
2
MEAN
98.5
0.6
0.1
0.0
0.8
0.0
SD
1.70
0.42
0.22
0.00
1.79
0.00
N
5
5
5
5
5
5
3
MEAN
96.4
1.7
0.0
0.0
1.8
0.1
SD
2.07
0.45
0.00
0.00
1.96
0.22
N
5
5
5
5
5
5
4
MEAN
96.6
2.4
0.4
0.0
0.5
0.1
SD
2.93
2.14
0.48
0.00
1
.oo
0.25
N
4
4
4
4
4
4
2
Appendix
D
(Table
2)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,I
,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Differential
Count
(%)

MALES
Group
No.
Animal
No.
Large
Mononuclear
Cells
(%)
Lymphocytes
%
Neutrophils
%
Eosinophils
(%)
Epithelial
Cells
(%)
Mitotic
Figures
(%)

1
61400
97.0
1
.o
0.0
0.0
2.0
0.0
61403
99.5
0.5
0.0
0.0
0.0
0.0
61404
100.0
0.0
0.0
0.0
0.0
0.0
61414
99.0
1
.o
0.0
0.0
0.0
0.0
61420
98.5
1.5
0.0
0.0
0.0
0.0
MEAN
98.8
0.8
0.0
0.0
0.4
0.0
SD
1.15
0.57
0.00
0.00
0.89
0.00
N
5
5
5
5
5
5
2
61398
100.0
0.0
0.0
0.0
0.0
0.0
61425
100.0
0.0
0.0
0.0
0.0
0.0
61435
91
.O
9.0
0.0
0.0
0.0
0.0
MEAN
97.0
3.0
0.0
0.0
0.0
0.0
SD
5.20
5.20
0.00
0.00
0.00
0.00
N
3
3
3
3
3
3
3
Appendix
D
(Table
2)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Fluid
Leukocyte
Differential
Count
(%)

MALES
Group
Animal
Large
Mononuclear
Epithelial
Cells
Mitotic
Figures
No.
Sex
No.
Cells
(%)
Lymphocytes
(%)
Neutrophils
(%)
Eosinophils
(%)
W
04
3
M
61407
100.0
0.0
0.0
0.0
0.0
0.0
M
61422
98.0
0.5
0.0
0.0
1.0
0.5
M
61424
97.5
2.0
0.0
0.0
0.5
0.0
M
61433
99.0
1.0
0.0
0.0
0.0
0.0
MEAN
98.6
0.9
0.0
0.0
0.4
0.1
SD
1.11
0.85
0.00
0.00
0.48
0.25
N
4
4
4
4
4
4
4
M
61418
98.5
0.5
1
.o
0.0
0.0
0.0
M
61423
100.0
0.0
0.0
0.0
0.0
0.0
M
61428
94.5
1.5
0.0
0.0
4.0
0.0
M
61429
97.0
1.0
0.0
0.5
0.0
1.5
M
61436
97.5
0.0
1.0
0.0
1.5
0.0
MEAN
97.5
0.6
0.4
0.1
1.1
0.3
SD
2.03
0.65
0.55
0.22
1.75
0.67
N
5
5
5
5
5
5
4
Appendix
D
(Table
2)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,I
,P­
Trichloroethane
(1
,I
,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Differential
Count
(%)

FEMALES
Group
No.

1
Animal
No.

61450
61464
61469
61490
61494
Large
Mononuclear
Cells
(%)

99.0
97.5
99.5
99.0
100.0
Lymphocytes
Neutrophils
Eosinophils
Epithelial
%
(70)
(33)
Cells
("
3~)

1.0
0.0
0.0
0.0
2.0
0.5
0.0
0.0
0.5
0.0
0.0
0.0
1.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
Mitotic
Figures
(%)

0.0
0.0
0.0
0.0
0.0
MEAN
99.0
0.9
0.1
0.0
0.0
0.0
SD
0.94
0.74
0.22
0.00
0.00
0.00
cn
N
5
5
5
5
5
5
2
61454
99.5
0.5
0.0
0.0
0.0
0.0
61457
99.0
1.0
0.0
0.0
0.0
0.0
61466
99.5
0.0
0.5
0.0
0.0
0.0
61487
95.5
0.5
0.0
0.0
4.0
0.0
61492
99.0
1.0
0.0
0.0
0.0
0.0
MEAN
98.5
0.6
0.1
0.0
0.8
0.0
SD
1.70
0.42
0.22
0.00
1.79
0.00
N
5
5
5
5
5
5
5
Appendix
D
(Table
2)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,1,2­
Trichloroethane
(1
,l
,PTCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Differential
Count
(%)

FEMALES
Group
Animal
No.
No.
Large
Mononuclear
Cells
(%)
Lymphocytes
(%)
Neutrophils
(%)
Eosinophils
%
Epithelial
Cells
(%)
Mitotic
Figures
(%)

3
61448
96.0
2.0
0.0
0.0
2.0
0.0
61463
98.0
2.0
0.0
0.0
0.0
0.0
61468
98.0
1
.o
0.0
0.0
0.5
0.5
61483
97.0
1.5
0.0
0.0
1.5
0.0
61489
93.0
2.0
0.0
0.0
5.0
0.0
MEAN
96.4
1.7
0.0
0.0
1.8
0.1
SD
2.07
0.45
0.00
0.00
1.96
0.22
N
5
5
5
5
5
5
4
61445
97.5
1.5
1
.o
0.0
0.0
0.0
61460
96.0
3.0
0.5
0.0
0.0
0.5
61478
93.0
5.0
0.0
0.0
2.0
0.0
61485
100.0
0.0
0.0
0.0
0.0
0.0
MEAN
96.6
2.4
0.4
0.0
0.5
0.1
SD
2.93
2.14
0.48
0.00
1
.oo
0.25
N
4
4
4
4
4
4
6
Appendix
D
(Table
3)
w
IL­
41
7001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
­
Summary
of
Means
MALES
Total
Cell
Large
Epithelial
Mitotic
Group
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
(Xl
O%/
ml)
Cells
(Xl
O%/
ml)
(Xl
OS/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(XlO%/
ml)
(XlO%
hl)

1
MEAN
1.7
1.7
0.012
0.000
0.000
0.006
0.000
SD
0.35
0.36
0.0082
0.0000
0.0000
0.0134
0.0000
N
5
5
5
5
5
5
5
2
MEAN
1.4
1.4
0.027
0.000
0.000
0.000
0.000
SD
0.50
0.54
0.0468
0.0000
0.0000
0.0000
0.0000
N
3
3
3
3
3
3
3
3
MEAN
1.5
1.5
0.013
0.000
0.000
0.006
0.002
SD
0.25
0.25
0.0127
0.0000
0.0000
0.0076
0.0040
N
4
4
4
4
4
4
4
4
MEAN
1.2
1.1
0.008
0.005
0.002
0.012
0.005
SD
0.36
0.35
0.0081
0.0066
0.0038
0.0177
0.0113
N
5
5
5
5
5
5
5
7
Appendix
D
(Table
3)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
­
Summary
of
Means
FEMALES
Total
Cell
Large
Epithelial
Mitotic
Group
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
(Xl
O­/
ml)
Cells
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
m/
ml)

1
MEAN
1
A
1.4
0.011
0.001
0.000
0.000
0.000
SD
0.96
0.96
0.0069
0.0020
0.0000
0.0000
0.0000
N
5
5
5
5
5
5
5
2
MEAN
0.8
0.8
0.005
0.001
0.000
0.004
0.000
SD
0.24
0.24
0.0045
0.0020
0.0000
0.0089
0.0000
2:
N
5
5
5
5
5
5
5
co
3
MEAN
1.0
1.0
0.018
0.000
0.000
0.019
0.001
SD
0.24
0.23
0.0068
0.0000
0.0000
0.0183
0.0020
N
5
5
5
5
5
5
5
4
MEAN
0.8
0.8
0.016
0.004
0.000
0.002
0.001
SD
0.33
0.33
0.0115
0.0057
0.0000
0.0040
0.0023
N
4
4
4
4
4
4
4
8
Appendix
D
(Table
4)
WIL­
417001
Acute
inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
MALES
Total
Cell
Large
Epithelial
Mitotic
Group
Animal
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
No.
(Xl
0*
6/
ml)
Cells
(Xl
O%/
ml)
(Xl
O%/
ml)
(XlO%/
ml)
(Xl
O/\
s/
ml)
(XlO%/
ml)
(XlO%/
ml)

1
61400
1.5
1.5
0.015
0.000
0.000
0.0300
0.0000
61403
1.7
1.7
0.009
0.000
0.000
0.0000
0.0000
61404
2.3
2.3
0.000
0.000
0.000
0.0000
0.0000
61414
1.7
1.7
0.017
0.000
0.000
0.000
0.000
61420
1.4
1.4
0.021
0.000
0.000
0.000
0.000
MEAN
1.7
1.7
0.012
0.000
0.000
0.006
0.000
SD
0.35
0.36
0.008
0.0000
0.0000
0.0134
0.0000
N
5
5
5
5
5
5
5
2
61398
1.9
1.9
0.000
0.000
0.000
0.000
0.000
61425
1.4
1.4
0.000
0.000
0.000
0.000
0.000
61435
0.9
0.8
0.081
0.000
0.000
0.000
0.000
MEAN
1.4
1.4
0.027
0.000
0.000
0.000
0.000
SD
0.50
0.54
0.0468
0.0000
0.0000
0.0000
0.0000
N
3
3
3
3
3
3
3
9
Appendix
D
(Table
4)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1
,1,2­
Trichloroethane
(1
,l
,P­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
MALES
Total
Cell
Large
Epithelial
Mitotic
Group
Animal
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
No.
(Xl
OS/
ml)
Cells
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
OYYml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
w/
ml)

3
61407
1.8
1.8
0.000
0.000
0.000
0.000
0.000
61422
1.6
1.6
0.008
0.000
0.000
0.016
0.008
61424
1.5
1.5
0.030
0.000
0.000
0.008
0.000
61433
1.2
1.2
0.012
0.000
0.000
0.000
0.000
MEAN
1.5
1.5
0.013
0.000
0.000
0.006
0.002
SD
0.25
0.25
0.0127
0.0000
0.0000
0.0076
0.0040
N
4
4
4
4
4
4
4
P
4
61418
1.2
1.2
0.006
0.012
0.000
0.000
0.000
61423
0.7
0.7
0.000
0.000
0.000
0.000
0.000
61428
1.0
0.9
0.015
0.000
0.000
0.040
0.000
61429
1.7
1.6
0.017
0.000
0.008
0.000
0.025
61436
1.2
1.2
0.000
0.012
0.000
0.018
0.000
MEAN
1.2
1.1
0.008
0.005
0.002
0.012
0.005
SD
0.36
0.35
0.0081
0.0066
0.0038
0.0177
0.0113
N
5
5
5
5
5
5
5
10
Appendix
D
(Table
4)
WIL­
417001
Acute
Inhalation
Toxicity
(With
Histopathology)
Study
of
1,
l
,PTrichloroethane
(1
,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
FEMALES
Total
Cell
Large
Epithelial
Mitotic
Group
Animal
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
No.
(Xi
O%/
ml)
Cells
(Xl
O%/
ml)
(Xl
OS/
ml)
(XlO%/
ml)
(Xl
m/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)

1
61450
1.1
1.1
0.011
0.000
0.000
0.000
0.000
61464
0.9
0.9
0.018
0.005
0.000
0.000
0.000
61469
3.1
3.1
0.016
0.000
0.000
0.000
0.000
61490
1.1
1.1
0.011
0.000
0.000
0.000
0.000
61494
0.8
0.8
0.000
0.000
0.000
0.000
0.000
MEAN
1.4
1.4
0.011
0.001
0.000
0.000
0.000
SD
0.96
0.96
0.0069
0.0020
0.0000
0.0000
0.0000
N
5
5
5
5
5
5
5
61454
0.6
0.6
0.003
0.000
0.000
0.000
0.000
2
61457
1.1
1.1
0.011
0.000
0.000
0.000
0.000
61466
0.9
0.9
0.000
0.005
0.000
0.000
0.000
61487
0.5
0.5
0.003
0.000
0.000
0.020
0.000
61492
0.8
0.8
0.008
0.000
0.000
0.000
0.000
MEAN
0.8
0.8
0.005
0.001
0.000
0.004
0.000
SD
0.24
0.24
0.0045
0.0020
0.0000
0.0089
0.0000
N
5
5
5
5
5
5
5
11
Appendix
D
(Table
4)
WIL­
417001
Acute
inhalation
Toxicity
(With
Histopathology)
Study
of
1
,I
,2­
Trichloroethane
(1,1,2­
TCE)
in
Rats
Sponsor:
HAP
Task
Force
Individual
Bronchoalveolar
Lavage
Fluid
Leukocyte
Counts
FEMALES
Total
Cell
Large
Epithelial
Mitotic
Group
Animal
Count
Mononuclear
Lymphocytes
Neutrophils
Eosinophils
Cells
Figures
No.
No.
(Xl
O%/
ml)
Cells
(Xl
0*
6/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(Xl
O%/
ml)
(X10/\
6/
ml)
(Xl
@%/
ml)

3
61448
1.4
1.3
0.028
0.000
0.000
0.028
0.000
61463
0.8
0.8
0.016
0.000
0.000
0.000
0.000
61468
0.9
0.9
0.009
0.000
0.000
0.005
0.005
61483
1.1
1.1
0.017
0.000
0.000
0.017
0.000
61489
0.9
0.8
0.018
0.000
0.000
0.045
0.000
MEAN
1.0
1.0
0.018
0.000
0.000
0.019
0.001
SD
0.24
0.23
0.0068
0.0000
0.0000
0.0183
0.0020
N
5
5
5
5
5
5
5
4
67445
1.2
1.2
0.018
0.012
0.000
0.000
0.000
61460
0.9
0.9
0.027
0.005
0.000
0.000
0.005
61478
0.4
0.4
0.020
0.000
0.000
0.008
0.000
61485
0.8
0.8
0.000
0.000
0.000
0.000
0.000
MEAN
0.8
0.8
0.016
0.004
0.000
0.002
0.001
SD
0.33
0.33
0.0115
0.0057
0.0000
0.0040
0.0023
N
4
4
4
4
4
4
4
12
WL­
417001
Hazardous
Air
Pollutant
Task
Force
Acute
Inhalation
Toxicity
(With
Histopathology)
S
tudv
of
1,1,2­
Trichloroethane
(1
.1.2­
TCE)
in
Rats
APPENDIXF
Results
of
Phagocytosis
and
Respiratory
Burst
Assays
(Gary
R.
Burleson,
Ph.
D.,
Burleson
Research
Technologies)

­264­
Inhalation
Immunotoxicology
Study
STUDY
NO.

BRT
20010222
(WIL
­
417001A)

CONDUCTED
FOR:

WIL
Research
Laboratories.
Inc.
1407
George
Road
Ashland.
OH
44805­
9281
AUTHORS:

Robin
M.
Palmatier.
B.
S.
Gary
R.
Burleson,
Ph.
D.

TESTING
FACILITY:

BRT
­
Burleson
Research
Technologies,
Inc.
5706
Chapel
Hill
Rd.
Raleigh,
NC
27607
DATE
OF
REPORT:

October
26,200l
BRT
­
Burksoa
Rcsrnrcb
Technologies,
inc.

Study
rqmn
#
BRT
200
10222
(WL­
41700
1)
Page
I
of
27
COMPLIANCE
STATEMENT
This
study
was
conducted
according
to
U.
S.
FDA
Good
Laboratory
Practices
for
Nonclioicai
Laboratory
Studies.
21
CFR
Part
58.

Burlesoo
Research
Technologies,
Inc.
Management
BRT
­
Burlesoa
Research
Technologies,
Inc.

Study
repon
#
BRT
200
IO222
(WIL­
4
1700
I
)
Page
2
of
27
266
QUALITY
ASSURANCE
STATEMENT
Report
Title:
Inhalation
Immunotoxicology
Study
(WIL
­417001A)

Sponsor:
WIL
Research
Laboratories,
Inc
1407
George
Road
Ashland,
OH
448059281
BRT
Project
Number:
BRT
20010222
Protocol
Number:
WIL
Research
Laboratories
Study
#
WIL
­
417001A
Study
Monitoc
Dr.
Dan
Kirkpatrick
WIL
Research
Laboratories.

Phase
Inspected
Date
of
Date
Report
Sent
Date
Report
Sent
Inspection
to
Investigator
to
Management
Sample
Receipt
and
Log
­in
02/
28/
01
03lO2101
03/
02/
01
Cell
Counts
Phagocytosis
Assay
Respiratory
Burst
Assay
03/
01/
01
03/
02/
O
1
03/
02IOl
Raw
Data
Audit
03/
14/
01
03/
20/
01
03/
20/
01
Draft
&
Final
Report
Audit
06/
08/
01
1
O/
26/
0
1
10/
26/
01
10/
26/
01
The
Final
Report
and
Data
Record
Audit
were
performed
by
BRT
Quality
Assurance
To
the
best
of
my
knowledge.
this
Final
Report
accurately
describes
the
Study
Methods
and
Rocadures
used.
and
the
reported
results
accurately
reflect
the
raw
data.

BRT
­
Burltsm
Research
Technologies,
Inc.

Study
report
#
BRT
20010222
(WiL­
417001~
Page
3
of
27
TABLE
OF
CONTENTS
TITLE
PAGE
.."..­.......
r......­....­....................­......................­.­.........................­.......­
......................
1
COMPLIANCE
STATEMENT
.......
.._._............­...­..................................................­­...............­
.....
2
QUALITY
ASSURANCE
STATEMENT..
.........
.._..................­.............".................­............­..­
.......
3
TABLE
OF
CONTENTS.
.._...............­­....­........""........................­.........­..............."................."
......
4
SUMMARY
....................
.."."............­.".............."......­..."......................­.......­
...................................
5
1.00B.
rEcT1vE..
.................................
.
.........
..­
..................................................
..."
.
.................
.
.
.....
7
2.0
STUDY
DESIGN
....
.
..................
I".
..............
.
...............
.
................................................................
8
2.1
SAMPLES..
......................................................................................................................................
8
2.2
SAMPLE
PREPARATION
..................................................................................................................
8
2.3
hIAGOCYTOSlS
ASSAY..
.................................................................................................................
8
2.4
RESPIRITORY
Bws~
Assny..
........................................................................................................
9
3.0
RESULTS
..............
...".
..............
.
..
.
.­..........__­........­.­.­..­
............
...".
......
.
..
.._...­
..........
.
.
.­
.......
10
3.1
PHAGoc\
T0S1S.
...........................................................................................................................
10
Table
I:
Mean
Phagocytic
Act&
i@.
................................................................................................
10
Figure
I:
Mean
Phagocytic
A&
vi@
...............................................................................................
10
Table
2:
Mean
Phagacytic
Activi@
lq
Sex
......................................................................................
I
I
figure
2:
Mean
Phagocytic
Acdvi@
by
Sex
....................................................................................
I
I
3.2
RESPIRATORY
Bmsr
...................................................................................................................
12
T&
e
3:
Mean
Respiratory
Bunt
for
Stimuioted
and
Non­
stimukted
Samples..
............................
I,
7
figure
3:
Mean
NonStimulmed
Respiratoty
Burst
.......................................................................
I­
7
Figure
4:
Mean
9bnulmed
Respiraiory
Buti
...............................................................................
13
Table
4:
Mean
Respirmoty
Burst
Aaivi@
(nmoks
Hfl/
ld
cells)
According
to
Ser
Within
Groaps
­
Non­
stimulmed
and
PM4­
Slimulmed..
......................................................................................
13
Figure
5:
Mean
Non­
stimulated
Respiratory
Burst
A&
4@
­By
Ser..
...........................................
I4
figure&
Mean
PMA­
Stimuimed
Respirmory
Burst
Actiti@
­
BySex..
..........................................
I4
4.0
DISCUS,
5ION
........................................
..­.....................­............."­
.............................................
15
5.0
DOCUMENTATION
AND
RETENTION
OF
DATA..
.................................................................
16
5.1
DESCRIPTION
OFCIRCUYSTANCE.!
i
AWECTING
DATA
QUALITY
OR
I%
TECRITI..
...........................
16
5.2
MAMTEXWCE
OF
RAW
DATA
......................................................................................................
!
6
5.3
DE~
IATIOSS
FROM
THE
SWDY
PLAN
............................................................................................
16
6.0
REFERENCES
."
.
..".
..............
I..
...............
..".
...........................................
..­
................................
17
APPENDIX
................
.
.
.
.......
.
.
.
.
.
..........
".
I
mm......."
..
...".
........
V.."
.
.
..................................................
18
APPENDIX
A
:
PHACOCXTX
Anrv~
n
­
I%
DI\
IDIJAL
SAMPLE
VALUES..
......................................
19
APPENDIX
B
:
RESP~~
UTORY
BURST
ACTMIY
­
L~
DWIDUAL
SAMPLE
VALUES..
.........................
.24
KEY
PERSONNEL
S1CNATUR.
E
PAGE
.................
..
I
......................................................................
27
BRT
­
BurIeson
Research
Tecbadogies.
he
~tudyrep~
ttlBRT20010222(
W1L417001)
Page
4
of27
SUMMARY
The
objecrive
of
this
study
was
to
evaluare
the
immunotoxiciry
of
1.1.2~
trkhloroethane
(1.1.2­
TCE)
in
rats
at
various
concertnations.
This
was
aswssed
by
measuring
phagocytosis
and
respiratory
burst
of
brochoalveolar
macrophages.

On
February
28
and
March
I,
200
1,
Burlescm
Research
Technologies
received
bronchoalveolar
large
samples
collected
by
WIL
Research
Laboratories.
Sample
viability
tanged
tian
97
­
100%.
The
samples
were
cenuititged
and
resuspended
at
concentrations
of
1
x
IO'
cells
per
ml.
Phagocytosis
and
Respiratory
Burst
assays
were
performed
on
each
sample
individually.

Animals
exposed
for
bronchoalveolar
lavage
evaluations
were
exposed
at
target
1,1,2­
TCE
concenaations
of
0,50.200
and
1500
ppm
for
male
rats
and
0,50,200
and
IO00
ppm
for
female
rats.
Actual
measured
concentrations
were
0,60,205
and
1474
ppm
and
0.45,
I77
and
840
ppm.
respectively.
for
males
and
females.
Exposure
of
rats
to
I,
I,
2­
TCE
by
inhalation
resulted
in
a
decrease
in
phagocytic
activity
by
alveolar
macrophages.
The
alveolar
macrophage
phagocytic
activity
ofmales
and
females
grouped
together
was
decreased
following
exposures
targeted
at
50
ppm,
200
ppm.
and
1500
or
1000
ppm
compared
to
control
animals
(0
ppm).

Exposure
of
males
to
1.
1.2­
TCE
resulted
in
a
decreased
phagocytic
activity
following
exposures
targeted
at
50
ppm.
200
ppm.
and
1500
ppm
compared
to
control
animals.
However.
only
exposure
targeted
at
200
ppm
appeared
to
affect
phagocytosis
in
alveolar
macrophages
from
female
rats.

Exposure
to
I,
I.
2­
TCE
by
inhalation
had
no
effect
on
the
respiratory
burst
activity
(neither
stimulated
nor
non­
stimulated)
by
alveolar
mauophages.
The
alveolar
macrophage
respiratay
burst
activity
of
males
and
females
grouped
together.
or
when
analyzed
separately
for
males
and
for
females.
was
not
affected
following
exposures
at
50
ppm.
200
ppm.
and
1500
or
IO00
ppm
compared
to
control
animals
(0
ppm).

The
respiratory
burst
activity,
both
stimulated
and
non­
stimulated.
was
greater
for
females
than
males.

In
summary.
inhalation
exposure
to
I,
I.
2­
TCE
suppressed
the
phagocytic
activity
of
alveolar
manophages
in
male.
but
not
female
rats.
However.
the
alveolar
macrophage
respiratory
burst
was
not
altered
by
exposure
to
1.1.2.
TCE
in
either
male
or
fkmale
rats.
The
differential
modulation
of
phagocytic
activity
and
respiratory
bust
activity
has
also
ken
observed
in
other
immunotoxicology
studies
(Rodgers
and
Xiong,

1993:
Rodgers
and
Xiatg,
19%)
and
emphasizes
the
need
to
evaluate
mere
than
one
cell
function
to
determine
the
possibility
of
immtmotoxicity.

BRT
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inc.

Study
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269
TITLE
OF
STUDY
Inhalation
lmmunotoxicology
Study
(WIL
­417001A)

SPONSOR
WIL
Research
Laboratories.
Inc.
1407
George
Road
Ashland.
OH
448059281
STUDY
MATERIAL(
S)

N/
A
RECEIPT
OF
SAMPLES
%
ANALYSIS
February
28
&
tvlarch
I,
2001
DATE
OF
DRAFT
REPORT
June
8.2001
DATE
OF
FINAL
REPORT
October
26.2001
lNVESTlCATWE
PERSONEL
Jacqueline
E.
Wiiliemr
B.
S.

Robin
M.
Palmatier.
B.
S.

BRT
­
Burieson
Research
Technologies,
Inc.

Studyreport#
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417001)
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of
27
270
1.0
OBJECTIVE
The
objective
of
this
study
was
to
evaluate
the
immunotoxicity
of
I.
1.2~
trichlorcethane
(I,
1,2­
TCE)
in
rats
at
various
concenoations.
Animals
exposed
for
bronchoalveolar
lavage
evaluations
were
exposed
at
target
I,
I&
TCE
concentrations
of
0.50.200
and
1500
ppm
for
male
rats
and
0.50.200
and
1000
ppm
for
female
rats.
Actual
measured
concentrations
were
0,60.205
and
1474
ppm
and
0.45.
177
and
840
ppm.

respectively,
for
malts
and
females.
This
was
assessed
by
measuring
phagocytosis
and
respiratory
burst
of
lxonchoalveolar
mauophages.

Phaeocvtosis:
The
appropriate
development
and
function
of
the
immune
system
is
crucial
in
maintaining
the
overafi
health
status
of
the
host.
The
concept
of
a
scavenger
cell
ofthe
extracellular
environment
in
search
of
debris
and
unwanted
material
dates
to
the
observations
of
Metchnikoff
(190.5)
who
introduced
the
term
"macrophage"
for
those
cells
that
had
phagocytic
characteristics
(Mctchnikoff.
1968).
Maf~
ophages
belong
to
the
mononuclear
phagocytic
system
(MPS).
or
the
reticuloendothelial
system.
which
consists
of
monocytes
and
tissue
macrophages.
Macrophages
are
present
in
virtually
al1
tissues
and
exert
a
modulatory
role
in
tissue
homeostasis.
initiation
and
generation
of
humoral
and
cell­
mediated
immunological
reactions.

and
inflarnmatcny
responses
(Unanue
and
Allen.
1987).
Thus,
alteration
of
macrophage
function
may
have
an
effect
on:
(I)
nonspecific
immtmiry.
(2)
inflammation.
(3)
cell­
mediated
immunity.
and
(4)
humoral
immunity.
Phagocytosis
is
a
functional
activity
shared
by
neutrophils
(polymorphonuclear
leukocytes),

monocytes
and
macrophages.
Measurement
of
the
macrophage
functional
phagocytic
activity
is
thus
an
important
immunotoxicological
and
immunophannacological
assessment
(Burieson
et
al..
1987).
The
process
ofphagccytosis
can
be
observed
and
quantified
in
these
cells
by
following
the
internalization
of
a
foreign
particle­
such
as
fluorescent­
labeled
bacteria.
This
technique
takes
advantage
of
the
detectability
ofthe
intracellular
fluorescence
emitted
by
the
engulfed
particles
(Wan
et
al.
1993),
as
well
as
the
effective
fluorescence
quenching
ofthe
extracellular
probe
by
trypan
blue
(Sahhn
et
al.
1983).
Phagocytosis
as
assayed
in
this
study
utilizes
fluorescein­
labeled
&scherichiu
coli
(K­
12
strain).
quenching
of
extracellular
label
by
trypan
blue,
and
quantification
using
a
fluorescence
96well
microplate
reader.
The
magninrde
of
phagocytosis
is
measured
by
the
fluorescence
intensity
associated
with
the
intracellular
fluorescent
particles.

Resuiratorv
Burst:
While
phagocytosis
measures
uptake
of
foreign
particles.
the
microbicidal
and
tumoricidal
activity
of
neuuophilr
mcnocytes,
and
mauophages
is
accomplished
by
metabolic
pathways
including
the
respiratory
burst.
Respiratory
burst
is
a
physiologic
response
to
soluble
and
particulate
agonias
consisting
of
the
production
of
superoxide
by
nicotinamide
adenine
dinucleotide
phosphate
(?
VADPIi)
oxidase.
Superoxide
generated
by
the
NADPH
ox&
se
is
rapidly
converted
to
H202
by
spootaneous
and
mzymatic
dismutation
(McCord
and
Fridovich,
1968;
McCord
and
Fridovich,
1969).

This
s~
dy
used
the
method
of
Rodgers
(1995)
to
assess
respiratory
burst
by
measuring
the
production
of
HzOz
using
a
fluorescent
probe
and
a
cytofluoromeuic
analysis.

BRT
­
Borlesoa
Rcsesreh
Technologies,
Inc.

Studyreport#
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WlL­
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7
of
27
271
2.0
STUDY
DESIGN
2.1
Samples
On
F&
NW
28,
and
March
I,
2001,17
and
19
alveolar
macrophage
samples
respectively
were
received
on
cold
packs
via
FedEx
Overnight.
Each
tube
was
labeled
with
the
WIL
study
number.
a
specimen
identification
number,
group
number,
and
the
sex
of
the
animal.
The
tubes
were
verified
against
the
Specimen
Identification
Record
smt
by
WIL
Research
Laboratories.
Al1
samples
received
on
a
given
day
were
processed
that
same
day.

2.2
Sample
Preparation
Sample
were
centrifuged
at
2000
rpm
for
S­
15
minutes
at
4
OC.
Tbe
supematant
was
discarded
and
the
pellet
resuspended
in
Dulbecco's
Modified
Eagle's
Medium
(DMEM)
with
10%
Fetal
Bovine
Serum
(FBS)
and
Penicillin/
Streptomycin
(P&
S).
Cell
counts
were
performed
using
a
Couher
Counter
21.
and
viability
was
evaluated
by
trypan
blue
exclusion.
Each
sample
was
adjusted
to
contain
I
x
10b
cells/
ml.
All
samples
had
viability
greater
than
97%.

23
Pbagacytosis
Assay
One
hundred
(100)
ul
of
each
alveolar
mauophage
cell
suspension
(1
x
1
O6
cells
per
ml
)
was
added
to
the
wells
of
a
96­
well
tissue
culture
plate,
for
a
final
cell
concentration
of
1
x
IO5
cells/
well.
50
ul
ofmedium
(DMEM/
FBSP&
S)
was
added
to
each
well.
Five
wells
containing
only
medium
were
used
as
plate
blanks.
Each
samples
or
blank
was
run
in
a
set
of
five
(5)

replicates.
The
cells
were
incubated
for
1
hour
at
37°
C
and
then
examined
under
a
microscope.

During
the
incubation
period.
the
fluorescent
hcofi
K­
12
bioparticle
suspension
(Moiecdar
Probes)
in
Hanks
Balanced
Salt
Solution
(HBSS)
was
prepared.
The
mixture
was
vortexed
and
sonicatcd.
Atkr
the
incubation
period.
the
plates
were
cemrifitged,
and
the
supematant
was
aspirated
by
vacuum
aspiration.
100
ul
ofthe
E.
coli/
HBSS
mixture
was
added
to
each
well
and
incubated
for
two
hours
at
37'C.

Following
incubation,
the
fluorescent
E.
coli
bioparticles
were
aspirated
by
vacuum
aspiration.
and
100
ul
of
trypan
blu&
itratebalanced
salt
soiution
(Molecular
Robes)
was
added
to
each
well
to
quench
the
fluorescence
of
extraccilular
biopatticles.
The
trypan
blue
was
then
quickly
removed
by
vacuum
aspiration
and
the
intracellular
fluorescence
generated
by
ingested
fluorescent
bioparticles
was
measured
using
a
Cytofluor
4OB9
fluorescence
microplate
reader
(Wavelengths:

excitatiat
­
485
run.
emission
­
530
run).

BRT
­
Burlesoa
Rcscnrcb
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Inc.

Study
report
#
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20010222
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417OOl)
Page
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of
27
272
Data
Caknlatioa
The
data
was
exported
from
the
Cytofluor
program
into
Microsoft
Excel.
From
Excel
the
plate
layout
was
copied
then
pasted
into
a
Softmax
Pro
file
(Molecular
Devices).
The
mean
fluorescence
immsiry
of
the
negative
con~
ol
wells
(medium
only)
was
calculated
to
obtain
the
plate
blank
value.
The
plate
blank
was
then
subttacted
from
all
other
wells
to
obtain
the
corrected
fluorescence
of
each
individual
experimental
well.
The
mean
corrected
tluorescena
ofthe
5
replicates
was
calculated
for
each
sample.
All
results
are
reponed
in
fluorescence
units.

2.4
Respimtory
Burst
Assay
Each
sample
was
assayed
in
2
sets
of
triplicates,
with
3
wells
stimulated
with
Phwbol
Myristate
Acetate
(PMA)
and
3
wells
non­
simulated
(no
PMA
added).
One
hundred
(100)
ul
of
each
cell
suspension
(1
x
lO'celis/
well)
was
added
to
a
96well
tissue
culture
plate.
Ninety
ul
of
5
uM
2.7­

Dicholorofluorescein
diacetate
(Molecular
Robes)
was
added
to
each
well
and
the
plate
was
incubated
at
37'C
to
allow
uptake
ofthe
subsnate
by
the
cells.
Following
incubation,
PMA
(Sigma)
was
added
to
triplicate
wells
(PMA­
stimulated
wells)
at
a
concennation
of
IO
r@
well
in
order
to
stimulate
oxygen
radical
production.
No
PMA
was
added
to
the
second
set
of
triplicate
(non­
stimulated)
wells.
The
plate
was
incubated
at
3PC.
Following
the
I­
hour
incubation.
200
ul
of
each
2.7­
dichlorofluorescein
Standard
(Poiysciences)
was
added
to
the
plate.
The
increase
in
fluorescent
product
was
then
measured
using
the
Cytofluor
4000
(PerSeptive
Biosystems)

fluorescence
microplate
reader
(Wavelengths:
excitation
­
485
nm.
emission
­
530
nm).

Data
Cakuhtioa
The
data
was
exported
from
the
Cytofluor
program
into
Microsoft
Excel.
From
Excel
the
pIale
layout
was
copied
then
pasted
into
a
Softmax
Pro
file
(Molecular
Devices).
Following
plate
blank
subtraction
(mean
zero
standard
values).
the
results
were
determined
by
interpolation
from
the
standard
curve.
All
results
are
expressed
as
nmoles
HQ
/
10'
cells.

BRT
­
BurIeson
Rcsearcb
Tecbaologks,
Inc.

Studyrepo~#
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417001)
Page
9
of
27
273
3.
ORESULTS
3.1
Pbsgacytasis
Exposure
to
1.
1.2­
TCE
by
inhalation
raulted
in
a
deuease
in
phagocytic
activity
by
alveolar
macrophages.
The
alveolar
macrophage
phagocytic
activity
of
males
and
females
grouped
together
was
decreased
following
exposures
targeted
at
SO
ppm.
200
ppm,
and
1500/
IO00
(males/
females)
ppm
compared
to
controi
animals
(0
ppm)
(Table
I
and
Figure
1).
The
mean
phagocytic
activity
for
each
sex
within
a
group
is
summarized
in
Table
2
and
Figure
2.
Exposure
of
males
to
I,
I.
2­
TCE
resulted
in
a
decreased
phagcqtic
activity
following
exposures
targeted
at
50
ppm.
200
ppm.
and
1500
ppm
compared
to
control
animals.
However,
anly
exposure
targeted
at
200
ppm
appeared
to
affect
phagocytosis
in
alveolar
mauophages
6om
female
rats
flable
2
and
Figure
2).

Tabk
1:
Mean
Pbagacytic
Activity
Treatment
Group
Mean
Pbngacytic
Activity
SE'
I
(Florascence
Units)
1
I
­Control
672
3.5
7
I­
­
Low
Concentration
490
24
3
­
Mid
Concenuaticm
350
22
I
4
­High
Concenoation
446
44
1
SE:
Smndird
Error
of
rk
Mean
Figure
1:
Mean
Pbngocytic
Activity
Phagocytic
Activity
BRT
­
Burksoa
Research
Tccbaologies,
Inc.

Study
report
#
BRT
20010222
(WlL­
217001)
Page
IO
of
27
274
Tabk
2:
Meoo
Pbagocytic
Activity
by
Sex
Mean
Pbsgoeytic
1
Treatment
croup
se1
Activity
(Floreaccnce
SE'
Units)
!
I
1
­
Control
Male
806
54
1
ConUOl
<
Female
538
27
'r
­

2
­
Low
Cmceluratim
Male
456
30
i
2
­
Low
Concenaaticm
Female
510
33
3
­
Mid
Concenaation
1
Male
I
286
I
25
I
3
­Mid
Concentration
Female
400
31
I
4
­
High
Concenuation
Male
1
443
62
/

4
­High
Concenuaticm
Fel­
llele
451
63
I
SE:
Srandmd
Error
qf
the
.Meon
Figure
2:
Mean
Pbagaytic
Activity
by
Sex
Phagocytic
Activity
BRT
­
Burleeoa
Reseereb
Technologies,
ine.

Study
report
#
ERT20010222(
WL417001)
Page
1
I
of27
275
