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      	UNITED STATES ENVIRONMENTAL PROTECTION AGENCY
       		WASHINGTON, D.C. 20460
      
      
      
      
                                      8/15/2012
                                                                               
                             OFFICE OF CHEMICAL SAFETY AND POLLUTION PREVENTION
MEMORANDUM

SUBJECT:	Product Chemistry Scoping Document for the Registration Review of Chemical Name.  

            Registration Review Case #:		4107
            PC Code:						116801, 116802
            CAS Numbers:					525-79-1
            Chemical Class:					Biochemical
                                                                  
FROM:		Sadaf Shaukat, Biologist /s/  8/15/12
            Biochemical Pesticides Branch
            Biopesticides & Pollution Prevention Division (7511P)

TO:			Cheryl Greene, Regulatory Action Leader
            Biochemical Pesticides Branch
            Biopesticides & Pollution Prevention Division (7511P) 




ACTION REQUESTED
	A preliminary assessment of available information on product chemistry data associated with the biopesticide cytokinins in support of the development of a Registration Review Work Plan.



Introduction

	The EPA has made the determination that there is adequate information available on cytokinins, including cytokinin, kinetin, and N[6]-benzyladenine, and does not foresee the need for additional biochemical pesticide product chemistry data for products that currently contain cytokinins as the technical grade active ingredient.  The three substances are plant growth regulators, which enhance the growth and development of plants. Cytokinin products have been registered with the EPA since 1978. Currently there are sixty-eight active registrations of products containing cytokinins. The Agency exempts cytokinins from the requirement of a tolerance when used as a plant regulator on plants, seeds, or cuttings and on all food commodities after harvest (see 40 CFR 180.1157). In addition, the Agency exempts N[6]-benzyladenine from the requirement of a tolerance when used in or on apple and pear at a rate of <=182 grams of active ingredient per acre per season, and in or on pistachio when applied at a rate of <=60 grams of active ingredient per acre per season (see 40 CFR 180.1150).


Concerning the product chemistry of cytokinins, all biochemical pesticide data requirements for the technical grade active ingredient have been satisfied and are summarized in Table 2. While no further product chemistry data are necessary for the products currently registered, future registrations must adequately address all of the biochemical pesticides product chemistry data requirements.







Table 1. Product Identity and Composition for Cytokinin/Kinetin
Common Name
Cytokinin/Kinetin
Chemical Name, IUPAC
N-(2-Furanylmethyl)-9H-purin-6-amine 
Molecular Weight
215.21 g/mol
PC Code
116801/116802
CAS Registry Number
525-79-1
Empirical Formula
C10H9N5O 
Registration Review Case Number
4107
Chemical Structure
                                       



Product Chemistry Summary
The Agency has conducted a review of the available product chemistry data and information.  These data are summarized below.

Table 2. Biochemical Pesticides Product Chemistry Data Requirements for Chemical Name
                                 Guideline No.
                       Physical and Chemical Properties
                                   Status[1]
                                     Value
                                     MRID/
                                    Source
830.1100
Product Identity and Composition
                                       A
Refer to Table 1.
N/A
830.1200
Description of starting materials, production and formulation process
                                       A
CBI
48541201
830.1400
Discussion of formation of impurities
                                       A
CBI
48541201
830.1700
Preliminary analysis
                                       A
CBI
48541202
830.6302
Color
                                       A
White
44911003
830.6303
Physical state
                                       A
Crystals
44911003
830.6304
Odor
                                       A
Odorless 
44911003
830.6313
Stability to normal and elevated temperatures, metals and metal ions
                                      N/A


830.7000
pH
                                       A
7.4 (25[o]C)
44911003
830.7050
UV/Visible light absorption

                                      N/A


830.7200
Melting point/melting range
                                       A
265-266 [o]C
44911003
830.7220
Boiling point/boiling range
                                       A
524.4 [0]C (at 760 mmHg)
LookChem
830.7300
Density
                                       A
1.4374 g/cm[3]
44911003
830.7520
Particle size, fiber length, and diameter distribution
                                      N/A


830.7550
830.7560
830.7570
Partition coefficient (n-Octanol/Water)
                                      N/A


830.7840
Water solubility 
                                       A
51.0 mg/L (25[o]C)
44911003
830.7950
Vapor pressure
                                       A
Negligible
FisherScien-tific
   1. A=Acceptable. N/A=Not Applicable. N/D=Not Determined


                                  References

Fisher Scientific, Online. 8/15/2012. http://www.fishersci.com/ecomm/servlet/msdsproxy?productName=BP9421&productDescription=KINETIN+1GM&catNo=BP942-1&vendorId=VN00033897&storeId=10652

LookChem, Online. 08/15/2012. http://www.lookchem.com/cas-525/525-79-1.html

Merck Index, Online. 08/14/2012.

Shi, Xiaoluo and Qin, Guohong. Color, Physical State, Odor, Melting Point, Density, Solubility and pH of Kinetin Technical. Private Study by LT BioSyn, Inc. Submitted to EPA. MRID 44911003.
	
