ARIZONA

PINK BOLLWORM ERADICATION PROGRAM STERILE MOTH BACKGROUND AND RELEASE
PROTOCOL

Central and Eastern counties in Arizona (Maricopa, Pinal, Pima, Graham,
Cochise and Greenlee) have been authorized to participate in season long
release of sterile Pink Bollworm moths in conjunction with entrance into
a regional Eradication Program.

BACKGROUND

The San Joaquin Valley of California has, despite annual introductions
of pink bollworm from the southern desert cotton growing areas,
prevented population establishment for 38 years through the release of
sterile pink bollworm moths on up to one million acres of cotton.  This
is true for a variety of reasons namely:

1.	Competitive effectiveness:

Studies by Miller et. al. compared and verified that both male and
female sterile pink bollworm moths are comparable to native moths in
mating responses (Miller et al. 1994). 

2.	Distribution:

Studies by Tabashnik et. al. reported that 66-94% of both native and
sterile male pink bollworm moths moved up to 400 meters from non-Bt to
Bt cotton (Tabashnik 1999).  This study suggests that sterile releases
in an eradication program targeted within 400 meters (1/4 mile) of Bt
cotton would provide pink bollworm population (males and females) within
the boundary for effective interaction with native moths (either Bt
susceptible or resistant).  Further data supplied by Keaveny, Program
Data Analyst, with the California Department of Food and Agriculture,
from the San Joaquin pink bollworm program verifies that targeted
sterile moths released on one mile corridors move effectively at least
one mile offsite for potential encounters with natives.  In fact, the
Keaveny study documents sterile recapture (at reduced levels) at 2-3
mile increments beyond the sterile release corridors further validating
the range of SIT releases.  The magnitude of trap recapture is also an
additional testament to the sterile males’ potential to respond to
pheromone from both traps and calling females.

PROTOCOL

The protocol for sterile release is as follows:

1.	Non-Bt Cotton:  Sterile moth releases are made three times per week
directly over non-Bt fields at a rate equivalent to 100 moths per acre
per day or higher.

2.	Bt Cotton:  SIT releases are made 2-3  times per week along one mile
corridors over Bt fields at a rate equivalent to 20 moths per acre per
day or higher.  This release rate represents a minimum of two times the
USDA/APHIS release rate for Bollgard and Bollgard II referenced in
Arizona’s 24 ( c ) label.  Corridors are offset by one half mile on
alternate release days to ensure that sterile moth populations are
maintained within one quarter mile of Bt fields at all times throughout
the season.

3.	All SIT releases are monitored through GPS assisted guidance systems
which produce flight recordings which are downloaded, printed and
reviewed daily.  These include flight paths and color coded designations
of all release operations.

4.	Arizona:  Allocation of sterile moths is 70 million moths per week
for an overall average of 10 million moths per day.  Releases are made
seven days a week and cover the period of May 1st through October 15,
2006.

5.	Pheromone traps on all Bt and non-Bt fields are serviced weekly and
counts of native vs. sterile moths are recorded by trained ID personnel
at each field office.  Because steriles are reared on a diet containing
red dye, either visual or simple chemical assays separate steriles from
natives.  

VOLUME 1

Document 2

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