Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Data
Requirement:
:
EPA
DP
Barcode
D288775
EPA
MRID
458677­
06
EPA
Guideline
70­
1(
Special
Study)

Test
material:
Purity:
{............}
Common
name:
Atrazine
Chemical
name:
IUPAC
CAS
name
6­
chloro­
N­
ethyl­
N'­(
1­
methylethyl)­
1,3,5­
triazine­
2,4­
diamine
CAS
No.
1912­
24­
9
Synonyms
EPA
PC
Code:
80803
Primary
Reviewer:
Thomas
M.
Steeger,
Ph.
D.,
Senior
Biologist
Date:
March
24,
2003
Environmental
Fate
and
Effects
Division,
ERB
4,
U.
S.
Environmental
Protection
Agency
Secondary
Reviewer(
s):
Joseph
E.
Tietge,
M.
S.,
Research
Aquatic
Biologist
Date:
Mid­
Continent
Ecology
Division,
National
Health
and
Environmental
Effects
Research
Laboratory
(
Duluth),
U.
S.
Environmental
Protection
Agency
Stephanie
Irene,
Ph.
D.,
Senior
Advisor
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
Mary
J.
Frankenberry,
Statistician
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
EPA
PC
Code
080803
Date
Evaluation
Completed:
05/
31/
2003
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
2
of
54
CITATION:
Sepulveda,
M.
S.
and
T.
S.
Gross.
2003.
Characterization
of
Atrazine
Exposures
and
Potential
Effects
in
Florida
Ecosystems
Dominated
by
Sugarcane
Agriculture:
A
Reconnaissance
Survey
of
Amphibians
in
South
Florida
for
the
Assessment
of
Potential
Atrazine
Effects.
Department
of
Physiological
Sciences,
University
of
Florida,
Caribbean
Science
Center,
Gainesville,
Florida.
Sponsor:
Syngenta
Crop
Protection,
Inc.
Study
ID:
ECORISK
Number
UFL­
02.
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
3
of
54
EXECUTIVE
SUMMARY:

A
reconnaissance
survey
for
cane
toads
(
Bufo
marinus)
in
south
Florida
sugarcane­
dominated
agricultural
sites
(
Belle
Glade
and
Canal
Point)
and
nonagricultural
sites
(
University
of
Miami)
indicated
increased
incidence
of
intersex
(
ovarian
tissue
in
Bidder's
organ)
in
frogs
identified
as
having
testes.
B.
marinus
is
typically
a
sexually
dimorphic
amphibian;
however,
100%
of
the
cane
toads
collected
at
Belle
Glade
and
roughly
55%
of
the
male/
intersex
frogs
collected
at
Canal
Point
exhibited
female
coloration.
Additionally,
males
typically
exhibit
nuptual
pads;
however
71%
and
0%
of
the
of
the
intersex
frogs
collected
from
Canal
Point
and
Belle
Glade,
respectively,
had
nuptual
pads.
Vitellogenin,
a
female­
specific
protein,
is
not
typically
expressed
in
males;
however,
intersex
frogs
had
vitellogenin
levels
(
774
±
29
PO4/
mg
protein)
similar
to
the
females
(
853
±
34
PO4/
mg
protein)
and
was
roughly
double
that
of
male
toads
(
375
±
34
PO4/
mg
protein).
Plasma
sex
steroids
(
17­
 
estradiol
and
testosterone)
were
relatively
gender­
specific;
however,
testosterone
levels
in
intersex
males
exhibited
roughly
twice
the
amount
of
variability
as
similar
estimates
for
males.
Although
agricultural
sites
had
atrazine
concentrations
ranging
from
<
0.01
to
24.45

g/
L
over
the
six­
month
sampling
period,
no
atrazine
levels
were
measured
at
the
University
of
Miami
(
nonagricultural)
site.

The
study
authors
clearly
recognized
the
potential
for
other
chemicals
to
confound
the
interpretation
of
this
study,
but
they
failed
to
monitor
for
any
other
chemicals.
The
study
authors
also
failed
to
characterize
atrazine
levels
in
nonagricultural
sites
and
water
quality
parameters
that
could
have
impacted
the
development
of
frogs.

In
this
study,
the
southern
toad
(
B.
terrestris)
was
also
examined
and
found
to
have
an
increased
incidence
of
intersex
(
Bidder's
organ
containing
ovarian
tissue)
in
both
agricultural
and
nonagricultural
sites.
The
study
authors
speculate
that
the
presence
of
a
Bidder's
organ
may
have
rendered
the
animals
more
sensitive
to
developmental
effects.
Bidder's
organ
is
characterized
as
a
nonfunctional,
rudimentary
ovary;
however,
no
information
is
available
on
whether
the
organ
has
an
endocrine
function
at
any
time
during
the
development
of
the
animal.
Additionally,
while
bufonids
typically
exhibit
Bidder's
organs
at
the
anterior
of
each
testis,
oogenesis
is
normally
abortive
because
the
oocytes
fail
to
reach
vitellogenesis
and
undergo
a
degenerative
process.
However,
the
one
slide
depicting
an
intersex
male
in
this
study
suggested
that
the
oocytes
possessed
yolk
and
were
therefore
vetellogenic.

This
study
was
useful
in
identifying
the
incidence
of
hermaphroditism
in
field­
collected
toads.
As
with
the
previous
studies,
toads
with
testes
also
appeared
to
have
ovarian
tissue,
but
unlike
previous
studies
the
ovarian
tissue
was
associated
with
the
Bidder's
organ
rather
than
the
testes.
While
toads
collected
in
agricultural
sites
may
have
been
exposed
to
atrazine
during
development,
it
is
unknown
whether
atrazine
was
present
at
the
nonagricultural
sites.
Other
problems
with
the
the
study
design
include
exposure
to
other
agrochemicals
and
environmental
conditions
relevant
to
development
of
toads,
e.
g.,
water
quality
characteristics.
These
factors
limit
an
interpretation
of
the
findings
and
make
it
difficult
to
establish
causality.
Based
on
these
data,
it
is
difficult
to
conclude
that
atrazine
exposure
was
associated
with
an
increased
incidence
of
intersex.
Also,
the
study
does
not
provide
insights
on
the
ecological
relevance
of
the
data.
If
toads
depend
on
coloration
to
attract
mates,
though,
toads
from
agricultural
sites
may
have
an
impaired
ability
to
attract
mates
because
of
their
distinctly
female
appearance.
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
4
of
54
I.
MATERIALS
AND
METHODS
GUIDELINE
FOLLOWED:
Nonguideline
Study
COMPLIANCE:
Not
conducted
under
GLP;
however,
most
practices
as
defined
by
40CFR
Part
160,
August
19,
1989,
were
established
for
this
study,
including
but
not
limited
to:
1.
Written,
authorized
preliminary
protocol
2.
Written,
authorized
Standard
Operating
Procedures
for
all
key
procedures.
3.
Organization
and
personnel
were
sufficient
in
terms
of
number,
education,
training
and
experience.
4.
Independent
QA
inspections
were
conducted
5.
Final
Report
was
written
6.
Raw
data,
documentation,
records,
protocols,
and
final
report
were
archived
A.
MATERIALS:

1.
Test
Material
[
Complete
this
subsection
using
the
information
provided
in
the
methodology
section
of
the
study
report.
Name
of
test
material
as
cited
in
the
study
report.]

Description:
Atrazine
Lot
No./
Batch
No.
:

Purity:
NA
Stability
of
compound
under
test
conditions:

Storage
conditions
of
NA
test
chemicals:

2.
Test
organism:

Species:
Cane
toad
(
Bufo
marinus);
Southern
toad
(
B.
terrestris);
Green
Treefrog
(
Hyla
cinera);
Cuban
Treefrog
(
Osteopilus.
septentrionalis)
.
Age
at
test
initiation:
Field
collected
animals;
age
not
determined
Weight
at
study
initiation:
(
mean
and
range)
Male:
125.1
±
8.3
g
Female:
133.9
±
10.3
g
Intersex:
123.0
±
14.5
g
Length
at
study
initiation:
(
mean
and
range)
Male:
100.6
±
2.5
mm
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
5
of
54
Female:
104.9
±
2.4
mm
Intersex:
99.9
±
6.7
mm
Source:
Field­
collected
at
Belle
Glade
and
Canal
Point
(
sugar
cane
sites);
University
of
Miami
(
nonagricultural
site)

B.
STUDY
DESIGN:

Objective:

1)
To
determine
whether
exposure
of
frogs
to
sugarcane
agricultural
areas
in
South
Florida
would
result
in
a
higher
incidence
of
intersex
and/
or
other
gonadal/
developmental
anomalies
2)
to
examine
amphibian
populations
exposed
to
a
complex
mixture
of
several
pesticides
and
assorted
agrichemicals.

1.
Experimental
Conditions
a)
Range­
finding
Study:
Preliminary
b)
Definitive
Study
Table
1
.
Experimental
Parameters
Parameter
Details
Acclimation:
period:
Conditions:
(
same
as
test
or
not)
Feeding:
Health:
(
any
mortality
observed)
NA
Duration
of
the
test
NA
Test
condition
static/
flow­
through
Type
of
dilution
system
for
flow­
through
method.

Renewal
rate
for
static
renewal
NA
Aeration,
if
any
NA
Test
vessel
NA
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Parameter
Details
Page
6
of
54
Source
of
dilution
water
Quality:
NA
Water
parameters:
Hardness
pH
Dissolved
oxygen
Total
Organic
carbon
Particulate
Matter
Metals
Pesticides
Chlorine
Temperature
{
Salinity
for
marine
or
estuarine
species}

Intervals
of
water
quality
measurement
NA
Number
of
replicates/
groups:
control:
solvent
control:
treated
ones:
NA
Number
of
organisms
per
replicate
/
groups:
control:
solvent
control:
treated
ones:
NA
Biomass
loading
rate
NA
Test
concentrations:
nominal:
measured:
NA
Solvent
(
type,
percentage,
if
used)
NA
Lighting
NA
Feeding
NA
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Parameter
Details
Page
7
of
54
Recovery
of
chemical
Level
of
Quantitation
Level
of
Detection
NA
Positive
control
{
if
used,
indicate
the
chemical
and
concentrations}
NA
Other
parameters,
if
any
NA
Reconnaissance
survey
was
conducted
in
the
field:

Initially,
distribution
and
concentration
of
atrazine
at
south
Florida
sites
(
Belle
Glade
and
Canal
Point)
was
measured
by
collecting
multiple
water
samples
from
several
canals/
ditches
every
2
weeks
from
February
through
June
with
an
additional
sample
in
mid­
July.
A
scoping
survey
of
atrazine
did
not
include
University
of
Miami
site.
No
chemicals
other
than
atrazine
were
screened.

A
scoping
survey
of
amphibians
for
Bufo
marinus
was
conducted;
however,
only
the
University
of
Miami
site
was
identified
as
nonagricultural
and
having
B.
marinus.

Although
B.
terrestris
was
located
at
Belle
Glade,
it
was
not
located
at
Canal
Point.
Two
nonagricultural
sites
(
Archibald
Biological
Station
and
Fisheater
Creek)
did
have
the
Southern
Toad.
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
8
of
54
2.
Observations:
Table
2:
Observations
Criteria
Details
Parameters
measured
including
the
sublethal
effects/
toxicity
symptoms
Observation
intervals
Were
raw
data
included?

Other
observations,
if
any
II.
RESULTS
and
DISCUSSION:
[
All
results
discussed
in
this
section
and
the
next
are
those
reported
by
the
study
authors.
Although
supplemental
data
are
typically
used
in
a
qualitative
manner
only,
EFED
verified
spreadsheet
data
and
ran
basic
statistical
analyses
on
the
major
study
parameters.
See
attached
appendix.
If
results
appeared
to
differ
in
any
substantive
way,
the
difference
was
reported
in
the
text
below.]

Canals
and
ditches
were
sampled
for
atrazine
at
both
of
the
agricultural
sites.
Atrazine
levels
ranged
from
<
0.10
to
24.45
ppb
at
Belle
Glade
and
from
<
0.10
to
19.54
ppb
at
Canal
Point.
Atrazine
concentration
at
Belle
Glade
(
consisting
of
8
sampling
sites)
peaked
in
early
February
(
February
10)
and
then
rapidly
declined
to
a
more
or
less
steady
state
for
the
remainder
of
the
sampling
period.
At
Canal
Point
(
consisting
of
8
sampling
sites),
atrazine
concentrations
peaked
on
March
16
and
again
on
April
14,
but
remained
sporadic.

Distribution
of
Amphibians
Bufo
marinus
was
located
in
two
sugarcane
areas
(
Belle
Glade
and
Canal
Point)
and
only
one
nonagricultural
area,
i.
e.,
University
of
Miami.
Bufo
terrestris
was
located
in
one
sugarcane
area
(
Belle
Glade)
and
two
nonagricultural
sites,
i.
e.,
Archibald
Biological
Station
and
Fisheater
Creek.
Hyla
cinera
was
present
at
Belle
Glade
and
Archibald.
O.
septentrionalis
was
present
at
Belle
Glade
and
Fisheating
Creek.

Body
weight,
length
and
coloration
were
recorded;
blood
was
collected
by
cardiac
puncture.

Gender
defined
by:
female:
present
of
ovarian
tissue
and
absence
of
testicular
tissue
male:
presence
of
testes
and
absence
of
developing
eggs
and/
or
ovarian
tissue
intersex:
presence
of
testes
and
developing
eggs
and/
or
ovarian
tissue.

Gonadal
Deformities:

segmented
testes:
testes
with
clear
demarcations
or
splits
highly
segmented:
testes
with
demarcations
making
testes
appear
multiple
abnormal
shaped:
twisted
or
curled
multiple
testes:
not
defined
in
text
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
9
of
54
intersex:
not
defined
in
text
but
likely
refers
to
mix
of
ovarian
and
testicular
tissue
in
same
organism
Only
testes
were
examined
histologically;
sections
were
fixed
in
formalin
and
then
embedded
in
parafin.
Approximately
29%
of
the
males
collected
from
Belle
Glade
and
39%
of
the
males
collected
from
Canal
Point
were
intersexed
based
on
ovarian
tissue
located
in
the
Bidder's
organ
of
males.

For
B.
terristris,
6
of
18
(
33%)
were
intersex
from
Archibaold
(
nonagricultural
site);
3
of
21
(
14%)
were
intersex
from
Belle
Glade;
and
5
of
23
(
22%)
were
intersex
from
Fisheater
Creek
(
cattle/
citrus
site).

Plasma
was
analyzed
for
17­
 
estradiol
and
testosterone
using
radioimmunoassay
and
for
alkali­
labile
phosphate
as
in
indirect
measure
of
vitellogenin.

Table
3
.
Distribution
of
amphibians
in
both
agricultural
(
sugarcane)
and
nonagricultural
sites.

Species
Agricultural
(
Sugarcane
Site)
Nonagricultural
Site
Belle
Glade
Canal
Point
Univ.
of
Miami
Archibald
Fisheater
Cane
Toad
(
B.
marinus)
X
X
X
Southern
Toad
(
B.
terrestris)
X
X
X
Green
Treefrog
(
Hyla
cinera)
X
X
Cuban
Treefrog
(
Ostropilus
septentrionalis)
X
X
C.
VERIFICATION
OF
STATISTICAL
RESULTS:
Basic
statistics
run
using
SAS
®
(
Statistical
Analysis
System,
Release
8.01,
Cary,
North
Carolina).
(
See
attached
printout).

D.
STUDY
DEFICIENCIES:
The
study
failed
to
measure
atrazine
in
nonagricultural
sites
and
to
collect
any
information
on
other
chemicals
present
at
the
sites.
The
study
also
failed
to
characterize
water
quality
at
any
of
the
collection
sites.

The
University
of
Miami
nonagricultural
site
was
added
late
in
the
study
and
was
therefore
not
included
in
any
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
10
of
54
of
the
atrazine
sampling
efforts.

According
to
the
protocol,
animals
collected
will
include:
tadpoles
at
early,
mid,
and
late
metamorphosis
(>
30
per
site),
metamorphs
(>
30
per
site),
and
adults
(
10
males
and
10
females
per
site)
as
available
for
each
species.
Each
site
will
contain
3
replicates/
location.
Blood
will
be
collected
for
subsequent
endocrine
biomarker
analysis
(
E,
T,
P
and
DHT).
Gonadal
tissue
from
adults
will
be
fixed
for
subsequent
evaluation
of
gonadal
tissues
and
laryngeal
development
and
subsequent
atrazine
analysis.
The
protocol
was
amended
to
show
that
only
adults
were
collected
because
tadpoles
were
"
difficult
to
collect."
Does
this
mean
that
tadpoles
were
too
few
in
number
to
collect?

No
effort
was
made
to
characterize
the
larynxes
because
the
technique
for
doing
so
had
not
been
validated
for
B.
marinus.

No
P
or
DHT
analysis
was
performed
for
B.
marinus
because
validated
procedures
were
not
available
for
this
species.

Proposed
experimental
start
date
on
UFL­
02
protocol
(
April
1,
2001)
does
not
agree
with
proposed
experimental
start
and
termination
date,
i.
e.,
March
1,
2002,
and
October
1,
2003,
respectively
also
reported
in
the
protocol
E.
REVIEWER'S
COMMENTS:

Although
the
study
objective
states
that
a
range
of
chemicals
are
used
on
sugarcane
and
implies
a
recognition
that
other
chemicals
would
potentially
confound
any
effort
to
link
anomalies
to
atrazine,
the
study
failed
to
measure
any
chemicals
in
the
nonagricultural
(
University
of
Miami)
site
and
only
measured
atrazine
in
each
of
the
agricultural
sites
(
Belle
Glade
and
Canal
Point).
Atrazine
concentrations
in
agricultural
sites
ranged
over
three
orders
of
magnitude.
While
some
sampling
sites
exhibited
relatively
high
atrazine
concentrations
during
a
sampling
date,
others
samples
collected
from
different
areas
of
the
same
site
exhibited
low
atrazine
concentrations.
Although
no
intersex
animals
were
detected
in
the
nonagricultural
site,
the
authors
suggested
that
the
incidence
of
intersex
in
agricultural
sites
may
be
reflective
of
"
background".
The
authors
also
suggested
that
Bidder's
organ
may
potentially
render
Bufonidae
more
susceptible
to
gonad
effects.
It
is
unclear
whether
the
characterization
of
Bidder's
organ
as
being
a
"
nonfunctional,
rudimentary
ovary"
is
entirely
correct
because
literature
suggests
that
if
normal
males
are
castrated,
the
Bidder's
organ
will
differentiate
into
an
ovary.
If
the
theory
that
atrazine
up­
regulates
is
correct
and
testosterone
is
transformed
into
estrogen,
is
there
sufficiently
low
testosterone
or
sufficiently
high
estrogen
to
result
in
differentiation
of
the
Bidder's
organ?

Across
treatment
sites,
estradiol
in
intersex
animals
was
only
slightly
(
302
±
33
pg/
mL)
above
levels
found
in
normal
males
(
293
±
23
pg/
mL);
serum
testosterone
in
intersex
males
was
slightly
lower
(
503
±
307
pg/
mL)
than
normal
males
(
552
±
174
pg/
mL).
It
is
clear,
though,
that
the
variability
in
testosterone
levels
for
intersex
males
(
CV=
61%)
was
roughly
double
that
of
normal
males
(
31.5%).
Additionally,
labile
phosphate
as
an
indirect
measure
of
plasma
vitellogenin
levels
in
intersex
males
(
774
±
29
PO4/
mg
protein)
was
roughly
double
the
level
in
normal
males
(
375
±
34
PO4/
mg
protein)
and
was
similar
to
the
levels
contained
in
females
(
853
±
34
PO4/
mg
protein).
If
the
labile
phosphate
(
ALP)
is
indicative
of
vitellogenin,
then
vitellogenesis
in
intersex
animals
has
been
up­
regulated
by
exposure
to
either
an
endogenous
and/
or
exogenous
estrogen.
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
1Petrini,
S.
and
F.
Zaccanti.
1998.
The
effects
of
Aromatize
and
5
 ­
reductase
inhibitors,
antiandrogen,
and
sex
steroids
on
Bidder's
organs
development
and
gonadal
differentiation
in
Bufo
bufo
tadpoles.
The
Journal
of
Experimental
Zoology
280:
245
­
259.

Page
11
of
54
It
is
unclear
why
the
bidder's
organ
makes
the
toad
a
generally
more
sensitive
species
to
the
effects
of
atrazine
or
other
endocrine
disrupting
chemicals.
It
could
be
argued
that
this
natural
hermaphroditic
condition
is
a
confounding
characteristic
since
the
ovarian
tissue
of
bidder's
organ
is
presumably
capable
of
estrogen
synthesis
and
therefore
capable
of
inducing
vitellogenin
synthesis
in
the
absence
of
an
exogenous
estrogen.
This
study
seems
to
indicate
that
vitellogenin
levels
in
the
intersex
organisms
approximates
the
normal
female
condition
in
the
absence
of
elevated
plasma
estrogen.
But,
the
lack
of
a
strong
vitellogenic
response
of
the
normal
males
from
the
same
site
suggests
that
they
have
not
been
exposed
to
an
estrogenic
chemical.
This
presumes
that
the
ALP­
measurement
is
a
good
surrogate
for
vitellogenin
and
that
the
male
levels
at
the
reference
site
represents
baseline
vitellogenin
concentrations.
This
issue
clearly
needs
more
research.

Furthermore,
although
Bufo
marinus
is
typically
a
sexually
dimorphic
species
with
gender­
specific
coloration
being
detected
in
the
nonagricultural
site
collected
animals,
female
coloration
pattern
was
detected
in
100%
of
the
frogs
(
male,
female
and
intersex)
collected
at
Belle
Glade
and
57%,
54%
and
100%
of
the
intersex,
male
and
female
frogs
collected
from
Canal
Point,
respectively.

Nuptual
pads
were
not
identified
for
females
from
any
of
the
sites.
In
males
collected
from
nonagricultural
sites,
90%
had
nuptual
pads,
while
82%
and
32%
of
the
males
had
nuptual
pads
from
Canal
Point
and
Belle
Glade,
respectively.
For
males
identified
as
intersex
from
Canal
Point
and
Belle
Glade,
71%
and
0%
had
nuptual
pads,
respectively.

Testicular
abnormalities
other
than
intersex
were
somewhat
similar
across
collection
sites
with
44%
for
Canal
Point,
61%
for
Belle
Glade
and
45%
for
University
of
Miami.

F.
CONCLUSIONS:
It
is
not
clear
why
the
authors
concluded
that
Bufo
marinus
may
be
more
sensitive
to
factors
that
influence
gonadal
development
and
sexual
differentiation
because
it
possesses
a
nonfunctional
rudimentary
ovary
(
Bidder's
organ).
For
that
reason,
the
species
may
be
a
useful
sentinel
for
factors
influencing
gonadal
development.
Additionally,
while
bufonids
typically
exhibit
Bidder's
organs
at
the
anterior
of
each
testis,
oogenesis
is
normally
abortive
because
the
oocytes
fail
to
reach
vitellogenesis
and
undergo
a
degenerative
process
(
Petrini
and
Zaccanti
1998)
1.
However,
the
one
slide
(
Figure
3F)
depicting
an
intersex
male
in
this
study
suggests
that
the
oocytes
possessed
yolk
and
were
therefore
vitellogenic.
Although
the
authors
state
that
previous
studies
have
hypothesized
a
potential
developmental
mechanism
of
action
for
atrazine
and
that
the
current
study
was
indirectly
designed
to
evaluate
a
similar
mechanism
of
action
in
amphibians,
the
current
study's
focus
on
adults
does
not
permit
direct
evaluation
of
this
effect.
Also,
it
is
difficult
to
test
the
hypothesis
that
atrazine
exposure
resulted
in
developmental
effects
in
amphibians
because
atrazine
was
not
measured
in
nonagricultural
sites,
other
chemicals
were
not
measured
at
any
of
the
sites,
and
no
water
quality
data
were
collected
to
characterize
the
study
sites.
While
it
may
have
been
possible
to
derive
some
correlation
between
the
incidence
of
intersex
in
males
and
atrazine
patterns
within
particular
areas
of
the
sample
sites,
these
data
are
not
provided.

Since
intersex,
i.
e.,
ovarian
tissue
in
the
Bidder's
organ,
did
not
appear
to
involve
the
testes
per
se,
it
is
unclear
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
12
of
54
whether
the
reproductive
capacity
of
the
adult
frogs
was
diminished
due
to
this
effect.
However,
the
intersex
frogs
appear
to
be
responding
to
an
environmental
estrogen
given
their
elevated
vitellogenin
levels
and
a
distinctly
female
appearance.
If
these
frogs
rely
heavily
of
coloration
to
attract
mate,
their
ability
to
reproduce
may
be
impaired
The
incidence
of
intersex
in
Bufo
terrestris
also
appeared
to
be
elevated;
however
this
was
true
for
both
agricultural
and
nonagricultural
sites.
The
authors
speculate
that
this
increased
incidence
may
be
indicative
of
the
variability
in
the
background
occurrence
of
intersex
among
amphibians
with
a
Bidder's
organ.
Since
atrazine
levels
at
the
nonagricultural
sites
were
not
characterized,
it
is
difficult
to
speculate
on
the
relevancy
of
the
proposition
of
"
background".
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
13
of
54
MEAN
LENGTH
WEIGHT
GONAD
AND
GONADOSOMATIC
INDEX
BY
SITE
AND
SEX
1
Obs
site
Sex
_
TYPE_
_
FREQ_
LENGTH
WEIGHT
GONAD
GSE
SD_
LT
SD_
WT
SD_
GONAD
SD_
GSI
1
BG
F
0
19
92.979
94.005
2.22374
1.11278
19.0811
67.773
4.3402
1.16438
2
BG
I
0
9
96.332
94.639
0.21622
0.23778
10.6652
40.275
0.0927
0.08913
3
BG
M
0
22
90.763
79.609
0.23955
0.31182
7.4531
21.577
0.2006
0.29333
4
CP
F
0
37
109.067
143.762
6.29178
3.35892
15.8198
65.904
10.8004
5.11631
5
CP
I
0
7
104.639
159.486
0.53771
0.37286
39.9476
59.198
0.1515
0.18901
6
CP
M
0
11
106.042
128.105
0.41200
0.32727
10.1743
47.890
0.1693
0.11934
7
UM
F
0
4
122.835
232.685
9.56100
4.33500
13.9482
136.862
5.1713
1.41630
8
UM
M
0
20
108.400
174.345
0.40530
0.23050
24.0066
57.599
0.1495
0.05375
ANOVA
FOR
LENGTH
BETWEEN
SITES
2
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
Dependent
Variable:
SVL
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
4629.32548
2314.66274
8.17
0.0008
Error
57
16146.86161
283.27827
Corrected
Total
59
20776.18709
R­
Square
Coeff
Var
Root
MSE
SVL
Mean
0.222819
16.04614
16.83087
104.8905
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
4629.325476
2314.662738
8.17
0.0008
Levene's
Test
for
Homogeneity
of
SVL
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
194186
97093.2
0.56
0.5751
Error
57
9906646
173801
Bartlett's
Test
for
Homogeneity
of
SVL
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
1.0035
0.6055
Dunnett's
t
Tests
for
SVL
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
57
Error
Mean
Square
283.2783
Critical
Value
of
Dunnett's
t
2.28365
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
UM
­
BG
29.856
8.712
51.000
***
CP
­
BG
16.088
5.240
26.936
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
14
of
54
ANOVA
FOR
LENGTH
BETWEEN
SITES
6
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
Dependent
Variable:
SVL
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
271.66953
271.66953
0.36
0.5566
Error
14
10484.85064
748.91790
Corrected
Total
15
10756.52018
R­
Square
Coeff
Var
Root
MSE
SVL
Mean
0.025256
27.37560
27.36636
99.96625
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
271.6695337
271.6695337
0.36
0.5566
Levene's
Test
for
Homogeneity
of
SVL
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
6318154
6318154
1.83
0.1976
Error
14
48349862
3453562
Bartlett's
Test
for
Homogeneity
of
SVL
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
9.8175
0.0017
Dunnett's
t
Tests
for
SVL
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
14
Error
Mean
Square
748.9179
Critical
Value
of
Dunnett's
t
2.14485
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
8.306
­
21.274
37.887
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
15
of
54
ANOVA
FOR
LENGTH
BETWEEN
SITES
10
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
Dependent
Variable:
SVL
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
3671.52388
1835.76194
6.98
0.0021
Error
50
13151.65680
263.03314
Corrected
Total
52
16823.18068
R­
Square
Coeff
Var
Root
MSE
SVL
Mean
0.218242
16.12326
16.21830
100.5894
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
3671.523883
1835.761942
6.98
0.0021
ANOVA
FOR
LENGTH
BETWEEN
SITES
12
Levene's
Test
for
Homogeneity
of
SVL
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
2890854
1445427
0.89
0.4168
Error
50
81136179
1622724
Bartlett's
Test
for
Homogeneity
of
SVL
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
26.2881
<.
0001
Dunnett's
t
Tests
for
SVL
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
50
Error
Mean
Square
263.0331
Critical
Value
of
Dunnett's
t
2.28707
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
UM
­
BG
17.637
6.177
29.097
***
CP
­
BG
15.279
1.582
28.976
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
16
of
54
NONPARAMETRIC
COMPARISON
OF
LENGTH
BETWEEN
SITES
14
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
363.0
579.50
62.927869
19.105263
CP
37
1276.0
1128.50
65.771701
34.486486
UM
4
191.0
122.00
33.744135
47.750000
Kruskal­
Wallis
Test
Chi­
Square
13.9187
DF
2
Pr
>
Chi­
Square
0.0009
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
4.0
9.50
1.816823
0.210526
CP
37
22.0
18.50
1.898929
0.594595
UM
4
4.0
2.00
0.974245
1.000000
Median
One­
Way
Analysis
Chi­
Square
11.4979
DF
2
Pr
>
Chi­
Square
0.0032
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
17
of
54
NONPARAMETRIC
COMPARISON
OF
LENGTH
BETWEEN
SITES
16
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
56.0
76.50
9.447222
6.222222
CP
7
80.0
59.50
9.447222
11.428571
Wilcoxon
Two­
Sample
Test
Statistic
80.0000
Normal
Approximation
Z
2.1170
One­
Sided
Pr
>
Z
0.0171
Two­
Sided
Pr
>
|
Z|
0.0343
t
Approximation
One­
Sided
Pr
>
Z
0.0257
Two­
Sided
Pr
>
|
Z|
0.0514
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
4.7087
DF
1
Pr
>
Chi­
Square
0.0300
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
2.0
4.50
1.024695
0.222222
CP
7
6.0
3.50
1.024695
0.857143
Median
Two­
Sample
Test
Statistic
6.0000
Z
2.4398
One­
Sided
Pr
>
Z
0.0073
Two­
Sided
Pr
>
|
Z|
0.0147
Median
One­
Way
Analysis
Chi­
Square
5.9524
DF
1
Pr
>
Chi­
Square
0.0147
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
18
of
54
NONPARAMETRIC
COMPARISON
OF
LENGTH
BETWEEN
SITES
18
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
313.0
594.0
55.398556
14.227273
CP
11
348.0
297.0
45.596052
31.636364
UM
20
770.0
540.0
54.497706
38.500000
Kruskal­
Wallis
Test
Chi­
Square
27.1304
DF
2
Pr
>
Chi­
Square
<.
0001
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
SVL
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
1.0
10.792453
1.810436
0.045455
CP
11
7.0
5.396226
1.490088
0.636364
UM
20
18.0
9.811321
1.780996
0.900000
Median
One­
Way
Analysis
Chi­
Square
31.1926
DF
2
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
19
of
54
ANOVA
FOR
WEIGHT
BETWEEN
SITES
20
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
Dependent
Variable:
wt
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
72873.3117
36436.6559
7.03
0.0019
Error
57
295228.9890
5179.4559
Corrected
Total
59
368102.3007
R­
Square
Coeff
Var
Root
MSE
wt
Mean
0.197970
53.73440
71.96844
133.9336
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
72873.31174
36436.65587
7.03
0.0019
Levene's
Test
for
Homogeneity
of
wt
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
3.5729E8
1.7864E8
2.91
0.0624
Error
57
3.4941E9
61300449
Bartlett's
Test
for
Homogeneity
of
wt
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
4.3547
0.1133
Dunnett's
t
Tests
for
wt
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
57
Error
Mean
Square
5179.456
Critical
Value
of
Dunnett's
t
2.28365
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
UM
­
BG
138.68
48.27
229.09
***
CP
­
BG
49.76
3.37
96.14
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
20
of
54
ANOVA
FOR
WEIGHT
BETWEEN
SITES
24
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
Dependent
Variable:
wt
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
16557.62363
16557.62363
6.82
0.0205
Error
14
34003.16446
2428.79746
Corrected
Total
15
50560.78809
R­
Square
Coeff
Var
Root
MSE
wt
Mean
0.327480
40.06429
49.28283
123.0094
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
16557.62363
16557.62363
6.82
0.0205
Levene's
Test
for
Homogeneity
of
wt
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
9605650
9605650
1.11
0.3100
Error
14
1.2117E8
8654926
Bartlett's
Test
for
Homogeneity
of
wt
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
0.9592
0.3274
Dunnett's
t
Tests
for
wt
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
14
Error
Mean
Square
2428.797
Critical
Value
of
Dunnett's
t
2.14485
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
CP
­
BG
64.85
11.58
118.12
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
21
of
54
ANOVA
FOR
WEIGHT
BETWEEN
SITES
28
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
Dependent
Variable:
wt
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
94122.4446
47061.2223
24.58
<.
0001
Error
50
95747.7804
1914.9556
Corrected
Total
52
189870.2250
R­
Square
Coeff
Var
Root
MSE
wt
Mean
0.495720
34.88999
43.76021
125.4234
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
94122.44456
47061.22228
24.58
<.
0001
Levene's
Test
for
Homogeneity
of
wt
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
77865494
38932747
3.38
0.0420
Error
50
5.7584E8
11516787
Bartlett's
Test
for
Homogeneity
of
wt
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
16.9429
0.0002
Dunnett's
t
Tests
for
wt
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
50
Error
Mean
Square
1914.956
Critical
Value
of
Dunnett's
t
2.28707
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
UM
­
BG
94.74
63.81
125.66
***
CP
­
BG
48.50
11.54
85.45
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
22
of
54
NONPARAMETRIC
COMPARISON
OF
WEIGHT
BETWEEN
SITES
32
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
371.0
579.50
62.926995
19.526316
CP
37
1270.0
1128.50
65.770787
34.324324
UM
4
189.0
122.00
33.743666
47.250000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
12.9558
DF
2
Pr
>
Chi­
Square
0.0015
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
3.0
9.50
1.816823
0.157895
CP
37
23.0
18.50
1.898929
0.621622
UM
4
4.0
2.00
0.974245
1.000000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
14.8325
DF
2
Pr
>
Chi­
Square
0.0006
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
23
of
54
NONPARAMETRIC
COMPARISON
OF
WEIGHT
BETWEEN
SITES
34
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
59.0
76.50
9.447222
6.555556
CP
7
77.0
59.50
9.447222
11.000000
Wilcoxon
Two­
Sample
Test
Statistic
77.0000
Normal
Approximation
Z
1.7995
One­
Sided
Pr
>
Z
0.0360
Two­
Sided
Pr
>
|
Z|
0.0719
t
Approximation
One­
Sided
Pr
>
Z
0.0460
Two­
Sided
Pr
>
|
Z|
0.0921
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
3.4314
DF
1
Pr
>
Chi­
Square
0.0640
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
3.0
4.50
1.024695
0.333333
CP
7
5.0
3.50
1.024695
0.714286
Median
Two­
Sample
Test
Statistic
5.0000
Z
1.4639
One­
Sided
Pr
>
Z
0.0716
Two­
Sided
Pr
>
|
Z|
0.1432
Median
One­
Way
Analysis
Chi­
Square
2.1429
DF
1
Pr
>
Chi­
Square
0.1432
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
24
of
54
NONPARAMETRIC
COMPARISON
OF
WEIGHT
BETWEEN
SITES
36
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
331.0
594.0
55.397439
15.045455
CP
11
313.0
297.0
45.595133
28.454545
UM
20
787.0
540.0
54.496608
39.350000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
26.0714
DF
2
Pr
>
Chi­
Square
<.
0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
wt
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
1.0
10.792453
1.810436
0.045455
CP
11
7.0
5.396226
1.490088
0.636364
UM
20
18.0
9.811321
1.780996
0.900000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
31.1926
DF
2
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
25
of
54
ANOVA
FOR
GONAD
WEIGHT
BETWEEN
SITES
38
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
Dependent
Variable:
gonad
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
288.453909
144.226955
1.78
0.1779
Error
57
4618.623706
81.028486
Corrected
Total
59
4907.077615
R­
Square
Coeff
Var
Root
MSE
gonad
Mean
0.058783
172.3940
9.001582
5.221517
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
288.4539090
144.2269545
1.78
0.1779
Levene's
Test
for
Homogeneity
of
gonad
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
128737
64368.3
1.67
0.1977
Error
57
2199727
38591.7
Bartlett's
Test
for
Homogeneity
of
gonad
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
15.4430
0.0004
Dunnett's
t
Tests
for
gonad
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
57
Error
Mean
Square
81.02849
Critical
Value
of
Dunnett's
t
2.28365
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
UM
­
BG
7.337
­
3.971
18.646
CP
­
BG
4.068
­
1.734
9.870
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
26
of
54
ANOVA
FOR
GONAD
WEIGHT
BETWEEN
SITES
42
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
Dependent
Variable:
gonad
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.40696877
0.40696877
27.59
0.0001
Error
14
0.20647098
0.01474793
Corrected
Total
15
0.61343975
R­
Square
Coeff
Var
Root
MSE
gonad
Mean
0.663421
34.02901
0.121441
0.356875
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
0.40696877
0.40696877
27.59
0.0001
Levene's
Test
for
Homogeneity
of
gonad
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
0.000571
0.000571
0.92
0.3534
Error
14
0.00868
0.000620
Bartlett's
Test
for
Homogeneity
of
gonad
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
1.5542
0.2125
Dunnett's
t
Tests
for
gonad
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
14
Error
Mean
Square
0.014748
Critical
Value
of
Dunnett's
t
2.14485
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
0.32149
0.19023
0.45276
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
27
of
54
ANOVA
FOR
GONAD
WEIGHT
BETWEEN
SITES
46
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
Dependent
Variable:
gonad
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
0.36407367
0.18203683
5.85
0.0052
Error
50
1.55651365
0.03113027
Corrected
Total
52
1.92058732
R­
Square
Coeff
Var
Root
MSE
gonad
Mean
0.189564
52.21800
0.176438
0.337887
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
0.36407367
0.18203683
5.85
0.0052
Levene's
Test
for
Homogeneity
of
gonad
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
0.00324
0.00162
0.27
0.7670
Error
50
0.3041
0.00608
Bartlett's
Test
for
Homogeneity
of
gonad
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
1.6748
0.4328
Dunnett's
t
Tests
for
gonad
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
50
Error
Mean
Square
0.03113
Critical
Value
of
Dunnett's
t
2.28707
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
0.17245
0.02344
0.32147
***
UM
­
BG
0.16575
0.04108
0.29043
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
28
of
54
NONPARAMETRIC
COMPARISON
OF
GONAD
WEIGHT
BETWEEN
SITES
50
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
346.0
579.50
62.926995
18.210526
CP
37
1282.0
1128.50
65.770787
34.648649
UM
4
202.0
122.00
33.743666
50.500000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
16.7428
DF
2
Pr
>
Chi­
Square
0.0002
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
4.0
9.50
1.816823
0.210526
CP
37
22.0
18.50
1.898929
0.594595
UM
4
4.0
2.00
0.974245
1.000000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
11.4979
DF
2
Pr
>
Chi­
Square
0.0032
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
29
of
54
NONPARAMETRIC
COMPARISON
OF
GONAD
WEIGHT
BETWEEN
SITES
52
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
48.0
76.50
9.433319
5.333333
CP
7
88.0
59.50
9.433319
12.571429
Average
scores
were
used
for
ties.

Wilcoxon
Two­
Sample
Test
Statistic
88.0000
Normal
Approximation
Z
2.9682
One­
Sided
Pr
>
Z
0.0015
Two­
Sided
Pr
>
|
Z|
0.0030
t
Approximation
One­
Sided
Pr
>
Z
0.0048
Two­
Sided
Pr
>
|
Z|
0.0096
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
9.1277
DF
1
Pr
>
Chi­
Square
0.0025
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
2.0
4.50
1.024695
0.222222
CP
7
6.0
3.50
1.024695
0.857143
Average
scores
were
used
for
ties.

Median
Two­
Sample
Test
Statistic
6.0000
Z
2.4398
One­
Sided
Pr
>
Z
0.0073
Two­
Sided
Pr
>
|
Z|
0.0147
Median
One­
Way
Analysis
Chi­
Square
5.9524
DF
1
Pr
>
Chi­
Square
0.0147
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
30
of
54
NONPARAMETRIC
COMPARISON
OF
GONAD
WEIGHT
BETWEEN
SITES
54
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
382.50
594.0
55.386271
17.386364
CP
11
377.50
297.0
45.585941
34.318182
UM
20
671.00
540.0
54.485621
33.550000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
14.5995
DF
2
Pr
>
Chi­
Square
0.0007
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
gonad
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
4.0
10.792453
1.810436
0.181818
CP
11
8.0
5.396226
1.490088
0.727273
UM
20
14.0
9.811321
1.780996
0.700000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
14.0970
DF
2
Pr
>
Chi­
Square
0.0009
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
31
of
54
ANOVA
FOR
GONADOSOMATIC
INDEX
BETWEEN
SITES
56
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
NOTE:
Due
to
missing
values,
only
59
observations
can
be
used
in
this
analysis.

Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
72.010680
36.005340
2.08
0.1350
Error
56
971.423418
17.346847
Corrected
Total
58
1043.434098
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.069013
152.0150
4.164955
2.739831
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
72.01068044
36.00534022
2.08
0.1350
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
8045.3
4022.6
1.28
0.2865
Error
56
176236
3147.1
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
32.7955
<.
0001
Dunnett's
t
Tests
for
GSI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
56
Error
Mean
Square
17.34685
Critical
Value
of
Dunnett's
t
2.28391
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
UM
­
BG
3.2222
­
2.0359
8.4804
CP
­
BG
2.2461
­
0.4875
4.9797
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
32
of
54
ANOVA
FOR
GONADOSOMATIC
INDEX
BETWEEN
SITES
60
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.07184534
0.07184534
3.62
0.0779
Error
14
0.27789841
0.01984989
Corrected
Total
15
0.34974375
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.205423
47.45756
0.140890
0.296875
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
0.07184534
0.07184534
3.62
0.0779
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
0.00219
0.00219
1.21
0.2896
Error
14
0.0253
0.00180
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
3.5402
0.0599
Dunnett's
t
Tests
for
GSI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
14
Error
Mean
Square
0.01985
Critical
Value
of
Dunnett's
t
2.14485
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
0.13508
­
0.01721
0.28737
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
33
of
54
ANOVA
FOR
GONADOSOMATIC
INDEX
BETWEEN
SITES
64
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
Dependent
Variable:
GSI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
0.09486143
0.04743072
1.18
0.3147
Error
50
2.00424045
0.04008481
Corrected
Total
52
2.09910189
R­
Square
Coeff
Var
Root
MSE
GSI
Mean
0.045191
70.41295
0.200212
0.284340
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
0.09486143
0.04743072
1.18
0.3147
Levene's
Test
for
Homogeneity
of
GSI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
0.0746
0.0373
1.60
0.2121
Error
50
1.1661
0.0233
Bartlett's
Test
for
Homogeneity
of
GSI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
42.9859
<.
0001
Dunnett's
t
Tests
for
GSI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
50
Error
Mean
Square
0.040085
Critical
Value
of
Dunnett's
t
2.28707
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
0.01545
­
0.15364
0.18454
UM
­
BG
­
0.08132
­
0.22279
0.06015
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
34
of
54
NONPARAMETRIC
COMPARISON
OF
GONADOSOMATIC
INDEX
(
GSI)
BETWEEN
SITES
68
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
18
306.50
540.0
60.741820
17.027778
CP
37
1265.50
1110.0
63.792823
34.202703
UM
4
198.00
120.0
33.164309
49.500000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
17.6412
DF
2
Pr
>
Chi­
Square
0.0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
18
4.0
8.847458
1.783289
0.222222
CP
37
21.0
18.186441
1.872862
0.567568
UM
4
4.0
1.966102
0.973655
1.000000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
10.0440
DF
2
Pr
>
Chi­
Square
0.0066
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
35
of
54
NONPARAMETRIC
COMPARISON
OF
GONADOSOMATIC
INDEX
(
GSI)
BETWEEN
SITES
70
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
56.50
76.50
9.440273
6.277778
CP
7
79.50
59.50
9.440273
11.357143
Average
scores
were
used
for
ties.

Wilcoxon
Two­
Sample
Test
Statistic
79.5000
Normal
Approximation
Z
2.0656
One­
Sided
Pr
>
Z
0.0194
Two­
Sided
Pr
>
|
Z|
0.0389
t
Approximation
One­
Sided
Pr
>
Z
0.0283
Two­
Sided
Pr
>
|
Z|
0.0566
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
4.4884
DF
1
Pr
>
Chi­
Square
0.0341
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
2.0
4.50
1.024695
0.222222
CP
7
6.0
3.50
1.024695
0.857143
Average
scores
were
used
for
ties.

Median
Two­
Sample
Test
Statistic
6.0000
Z
2.4398
One­
Sided
Pr
>
Z
0.0073
Two­
Sided
Pr
>
|
Z|
0.0147
Median
One­
Way
Analysis
Chi­
Square
5.9524
DF
1
Pr
>
Chi­
Square
0.0147
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
36
of
54
NONPARAMETRIC
COMPARISON
OF
GONADOSOMATIC
INDEX
(
GSI)
BETWEEN
SITES
72
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
524.50
594.0
55.289008
23.840909
CP
11
423.00
297.0
45.505889
38.454545
UM
20
483.50
540.0
54.389940
24.175000
Average
scores
were
used
for
ties.

Kruskal­
Wallis
Test
Chi­
Square
7.6716
DF
2
Pr
>
Chi­
Square
0.0216
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
GSI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
10.00
10.792453
1.758432
0.454545
CP
11
8.50
5.396226
1.447286
0.772727
UM
20
7.50
9.811321
1.729838
0.375000
Average
scores
were
used
for
ties.

Median
One­
Way
Analysis
Chi­
Square
4.8749
DF
2
Pr
>
Chi­
Square
0.0874
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
37
of
54
ANOVA
FOR
CONDITION
INDEX
BETWEEN
SITES
74
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
Dependent
Variable:
CI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
2.87929138
1.43964569
6.71
0.0024
Error
57
12.22812021
0.21452842
Corrected
Total
59
15.10741159
R­
Square
Coeff
Var
Root
MSE
CI
Mean
0.190588
38.52713
0.463172
1.202197
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
2.87929138
1.43964569
6.71
0.0024
Levene's
Test
for
Homogeneity
of
CI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
0.6135
0.3067
3.66
0.0320
Error
57
4.7813
0.0839
Bartlett's
Test
for
Homogeneity
of
CI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
4.3397
0.1142
Dunnett's
t
Tests
for
CI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
57
Error
Mean
Square
0.214528
Critical
Value
of
Dunnett's
t
2.28365
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
UM
­
BG
0.8712
0.2893
1.4531
***
CP
­
BG
0.3133
0.0148
0.6119
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
38
of
54
ANOVA
FOR
CONDITION
INDEX
BETWEEN
SITES
78
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
Dependent
Variable:
CI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
8.11930253
8.11930253
2.32
0.1499
Error
14
48.97043484
3.49788820
Corrected
Total
15
57.08973737
R­
Square
Coeff
Var
Root
MSE
CI
Mean
0.142220
118.0374
1.870264
1.584467
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
8.11930253
8.11930253
2.32
0.1499
Levene's
Test
for
Homogeneity
of
CI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
183.9
183.9
1.95
0.1844
Error
14
1320.7
94.3380
Bartlett's
Test
for
Homogeneity
of
CI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
23.5316
<.
0001
Dunnett's
t
Tests
for
CI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
14
Error
Mean
Square
3.497888
Critical
Value
of
Dunnett's
t
2.14485
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
1.4360
­
0.5856
3.4576
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
39
of
54
ANOVA
FOR
CONDITION
INDEX
BETWEEN
SITES
82
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
Dependent
Variable:
CI
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
22.6025084
11.3012542
2.26
0.1149
Error
50
250.0105867
5.0002117
Corrected
Total
52
272.6130951
R­
Square
Coeff
Var
Root
MSE
CI
Mean
0.082911
151.8343
2.236115
1.472734
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
22.60250839
11.30125420
2.26
0.1149
Levene's
Test
for
Homogeneity
of
CI
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
1898.9
949.5
0.93
0.4022
Error
50
51177.9
1023.6
Bartlett's
Test
for
Homogeneity
of
CI
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
122.7
<.
0001
Dunnett's
t
Tests
for
CI
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
50
Error
Mean
Square
5.000212
Critical
Value
of
Dunnett's
t
2.28707
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
UM
­
BG
1.4305
­
0.1496
3.0105
CP
­
BG
0.3156
­
1.5730
2.2041
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
40
of
54
NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
(
CI)
BETWEEN
SITES
86
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
393.0
579.50
62.927869
20.684211
CP
37
1253.0
1128.50
65.771701
33.864865
UM
4
184.0
122.00
33.744135
46.000000
Kruskal­
Wallis
Test
Chi­
Square
10.5265
DF
2
Pr
>
Chi­
Square
0.0052
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
19
4.0
9.50
1.816823
0.210526
CP
37
23.0
18.50
1.898929
0.621622
UM
4
3.0
2.00
0.974245
0.750000
Median
One­
Way
Analysis
Chi­
Square
9.3983
DF
2
Pr
>
Chi­
Square
0.0091
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
41
of
54
NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
(
CI)
BETWEEN
SITES
88
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
60.0
76.50
9.447222
6.666667
CP
7
76.0
59.50
9.447222
10.857143
Wilcoxon
Two­
Sample
Test
Statistic
76.0000
Normal
Approximation
Z
1.6936
One­
Sided
Pr
>
Z
0.0452
Two­
Sided
Pr
>
|
Z|
0.0903
t
Approximation
One­
Sided
Pr
>
Z
0.0555
Two­
Sided
Pr
>
|
Z|
0.1110
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
3.0504
DF
1
Pr
>
Chi­
Square
0.0807
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
3.0
4.50
1.024695
0.333333
CP
7
5.0
3.50
1.024695
0.714286
Median
Two­
Sample
Test
Statistic
5.0000
Z
1.4639
One­
Sided
Pr
>
Z
0.0716
Two­
Sided
Pr
>
|
Z|
0.1432
Median
One­
Way
Analysis
Chi­
Square
2.1429
DF
1
Pr
>
Chi­
Square
0.1432
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
42
of
54
NONPARAMETRIC
COMPARISON
OF
CONDITION
INDEX
(
CI)
BETWEEN
SITES
90
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
349.0
594.0
55.398556
15.863636
CP
11
305.0
297.0
45.596052
27.727273
UM
20
777.0
540.0
54.497706
38.850000
Kruskal­
Wallis
Test
Chi­
Square
23.2397
DF
2
Pr
>
Chi­
Square
<.
0001
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
CI
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
1.0
10.792453
1.810436
0.045455
CP
11
7.0
5.396226
1.490088
0.636364
UM
20
18.0
9.811321
1.780996
0.900000
Median
One­
Way
Analysis
Chi­
Square
31.1926
DF
2
Pr
>
Chi­
Square
<.
0001
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
43
of
54
ANOVA
FOR
E2/
T
RATIO
BETWEEN
SITES
92
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
NOTE:
Due
to
missing
values,
only
42
observations
can
be
used
in
this
analysis.

Dependent
Variable:
RATIO
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
21.055872
21.055872
0.15
0.6999
Error
40
5587.442993
139.686075
Corrected
Total
41
5608.498865
R­
Square
Coeff
Var
Root
MSE
RATIO
Mean
0.003754
161.1670
11.81889
7.333315
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
21.05587231
21.05587231
0.15
0.6999
Levene's
Test
for
Homogeneity
of
RATIO
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
53436.8
53436.8
0.32
0.5745
Error
40
6670870
166772
Bartlett's
Test
for
Homogeneity
of
RATIO
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
1.4679
0.2257
Dunnett's
t
Tests
for
RATIO
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
40
Error
Mean
Square
139.6861
Critical
Value
of
Dunnett's
t
2.02111
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
CP
­
BG
1.478
­
6.215
9.170
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
44
of
54
ANOVA
FOR
E2/
T
RATIO
BETWEEN
SITES
96
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
NOTE:
Due
to
missing
values,
only
15
observations
can
be
used
in
this
analysis.

Dependent
Variable:
RATIO
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
3.54538874
3.54538874
0.57
0.4636
Error
13
80.81750386
6.21673107
Corrected
Total
14
84.36289260
R­
Square
Coeff
Var
Root
MSE
RATIO
Mean
0.042025
100.0048
2.493337
2.493217
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
3.54538874
3.54538874
0.57
0.4636
Levene's
Test
for
Homogeneity
of
RATIO
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
7.8877
7.8877
0.13
0.7221
Error
13
776.1
59.6964
Bartlett's
Test
for
Homogeneity
of
RATIO
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
0.0674
0.7951
Dunnett's
t
Tests
for
RATIO
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
13
Error
Mean
Square
6.216731
Critical
Value
of
Dunnett's
t
2.16042
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
­
0.9924
­
3.8314
1.8466
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
45
of
54
ANOVA
FOR
E2/
T
RATIO
BETWEEN
SITES
100
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
NOTE:
Due
to
missing
values,
only
42
observations
can
be
used
in
this
analysis.

Dependent
Variable:
RATIO
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
6.0121986
3.0060993
0.88
0.4222
Error
39
132.9691436
3.4094652
Corrected
Total
41
138.9813422
R­
Square
Coeff
Var
Root
MSE
RATIO
Mean
0.043259
132.2498
1.846474
1.396201
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
6.01219864
3.00609932
0.88
0.4222
Levene's
Test
for
Homogeneity
of
RATIO
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
148.8
74.3982
0.47
0.6311
Error
39
6230.2
159.7
Bartlett's
Test
for
Homogeneity
of
RATIO
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
9.7484
0.0076
Dunnett's
t
Tests
for
RATIO
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.
Alpha
0.05
Error
Degrees
of
Freedom
39
Error
Mean
Square
3.409465
Critical
Value
of
Dunnett's
t
2.31338
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
CP
­
BG
­
0.3223
­
2.0859
1.4413
UM
­
BG
­
0.8799
­
2.4128
0.6531
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
46
of
54
NONPARAMETRIC
COMPARISON
OF
E2/
T
RATIO
BETWEEN
SITES
104
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
15
269.0
322.50
38.095275
17.933333
CP
27
634.0
580.50
38.095275
23.481481
Wilcoxon
Two­
Sample
Test
Statistic
269.0000
Normal
Approximation
Z
­
1.3912
One­
Sided
Pr
<
Z
0.0821
Two­
Sided
Pr
>
|
Z|
0.1642
t
Approximation
One­
Sided
Pr
<
Z
0.0858
Two­
Sided
Pr
>
|
Z|
0.1717
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
1.9723
DF
1
Pr
>
Chi­
Square
0.1602
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
15
5.0
7.50
1.571468
0.333333
CP
27
16.0
13.50
1.571468
0.592593
Median
Two­
Sample
Test
Statistic
5.0000
Z
­
1.5909
One­
Sided
Pr
<
Z
0.0558
Two­
Sided
Pr
>
|
Z|
0.1116
Median
One­
Way
Analysis
Chi­
Square
2.5309
DF
1
Pr
>
Chi­
Square
0.1116
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
47
of
54
NONPARAMETRIC
COMPARISON
OF
E2/
T
RATIO
BETWEEN
SITES
106
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
83.0
72.0
8.485281
9.222222
CP
6
37.0
48.0
8.485281
6.166667
Wilcoxon
Two­
Sample
Test
Statistic
37.0000
Normal
Approximation
Z
­
1.2374
One­
Sided
Pr
<
Z
0.1080
Two­
Sided
Pr
>
|
Z|
0.2159
t
Approximation
One­
Sided
Pr
<
Z
0.1181
Two­
Sided
Pr
>
|
Z|
0.2363
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
1.6806
DF
1
Pr
>
Chi­
Square
0.1949
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
5.0
4.20
0.979796
0.555556
CP
6
2.0
2.80
0.979796
0.333333
Median
Two­
Sample
Test
Statistic
2.0000
Z
­
0.8165
One­
Sided
Pr
<
Z
0.2071
Two­
Sided
Pr
>
|
Z|
0.4142
Median
One­
Way
Analysis
Chi­
Square
0.6667
DF
1
Pr
>
Chi­
Square
0.4142
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
48
of
54
NONPARAMETRIC
COMPARISON
OF
E2/
T
RATIO
BETWEEN
SITES
108
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
542.0
473.0
39.707262
24.636364
CP
8
157.0
172.0
31.219652
19.625000
UM
12
204.0
258.0
35.916570
17.000000
Kruskal­
Wallis
Test
Chi­
Square
3.2394
DF
2
Pr
>
Chi­
Square
0.1980
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
RATIO
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
22
14.0
11.0
1.637964
0.636364
CP
8
4.0
4.0
1.287842
0.500000
UM
12
3.0
6.0
1.481594
0.250000
Median
One­
Way
Analysis
Chi­
Square
4.5260
DF
2
Pr
>
Chi­
Square
0.1040
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
49
of
54
ANOVA
FOR
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
110
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
60
NOTE:
Due
to
missing
values,
only
20
observations
can
be
used
in
this
analysis.

Dependent
Variable:
P_
mg
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
11649.5373
5824.7687
0.32
0.7294
Error
17
308087.4127
18122.7890
Corrected
Total
19
319736.9500
R­
Square
Coeff
Var
Root
MSE
P_
mg
Mean
0.036435
15.97021
134.6209
842.9500
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
11649.53730
5824.76865
0.32
0.7294
Levene's
Test
for
Homogeneity
of
P_
mg
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
33916350
16958175
0.05
0.9474
Error
17
5.3185E9
3.1285E8
Bartlett's
Test
for
Homogeneity
of
P_
mg
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
0.0751
0.9631
Dunnett's
t
Tests
for
P_
mg
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
17
Error
Mean
Square
18122.79
Critical
Value
of
Dunnett's
t
2.42696
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
CP
­
BG
54.37
­
110.29
219.02
UM
­
BG
25.72
­
170.61
222.06
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
50
of
54
ANOVA
FOR
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
114
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
2
BG
CP
Number
of
observations
16
NOTE:
Due
to
missing
values,
only
14
observations
can
be
used
in
this
analysis.
Dependent
Variable:
P_
mg
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
532.1488
532.1488
0.04
0.8535
Error
12
179378.2083
14948.1840
Corrected
Total
13
179910.3571
R­
Square
Coeff
Var
Root
MSE
P_
mg
Mean
0.002958
15.80060
122.2628
773.7857
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
1
532.1488095
532.1488095
0.04
0.8535
Levene's
Test
for
Homogeneity
of
P_
mg
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
1
75518806
75518806
0.30
0.5920
Error
12
2.9897E9
2.4914E8
Bartlett's
Test
for
Homogeneity
of
P_
mg
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
1
0.1445
0.7038
Dunnett's
t
Tests
for
P_
mg
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
12
Error
Mean
Square
14948.18
Critical
Value
of
Dunnett's
t
2.17886
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
site
Between
95%
Confidence
Comparison
Means
Limits
CP
­
BG
­
12.46
­
156.33
131.41
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
51
of
54
ANOVA
FOR
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
118
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
site
3
BG
CP
UM
Number
of
observations
53
NOTE:
Due
to
missing
values,
only
24
observations
can
be
used
in
this
analysis.

Dependent
Variable:
P_
mg
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
2
182143.0833
91071.5417
8.25
0.0023
Error
21
231848.7500
11040.4167
Corrected
Total
23
413991.8333
R­
Square
Coeff
Var
Root
MSE
P_
mg
Mean
0.439968
27.98847
105.0734
375.4167
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
site
2
182143.0833
91071.5417
8.25
0.0023
Levene's
Test
for
Homogeneity
of
P_
mg
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
site
2
5.3083E8
2.6541E8
0.75
0.4831
Error
21
7.399E9
3.5233E8
Bartlett's
Test
for
Homogeneity
of
P_
mg
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
site
2
2.8489
0.2406
Dunnett's
t
Tests
for
P_
mg
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
21
Error
Mean
Square
11040.42
Critical
Value
of
Dunnett's
t
2.37033
Minimum
Significant
Difference
124.53
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
site
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
CP
­
BG
51.63
­
72.90
176.15
UM
­
BG
­
153.50
­
278.03
­
28.97
***
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
52
of
54
NONPARAMETRIC
COMPARISON
OF
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
122
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
84.0
94.50
13.162447
9.333333
CP
7
81.0
73.50
12.619429
11.571429
UM
4
45.0
42.00
10.583005
11.250000
Kruskal­
Wallis
Test
Chi­
Square
0.6439
DF
2
Pr
>
Chi­
Square
0.7247
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
9
4.0
4.50
1.141329
0.444444
CP
7
4.0
3.50
1.094243
0.571429
UM
4
2.0
2.00
0.917663
0.500000
Median
One­
Way
Analysis
Chi­
Square
0.2413
DF
2
Pr
>
Chi­
Square
0.8864
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
53
of
54
NONPARAMETRIC
COMPARISON
OF
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
124
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
I
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
8
61.0
60.0
7.745967
7.625000
CP
6
44.0
45.0
7.745967
7.333333
Wilcoxon
Two­
Sample
Test
Statistic
44.0000
Normal
Approximation
Z
­
0.0645
One­
Sided
Pr
<
Z
0.4743
Two­
Sided
Pr
>
|
Z|
0.9485
t
Approximation
One­
Sided
Pr
<
Z
0.4748
Two­
Sided
Pr
>
|
Z|
0.9495
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0167
DF
1
Pr
>
Chi­
Square
0.8973
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
8
4.0
4.0
0.960769
0.50
CP
6
3.0
3.0
0.960769
0.50
Median
Two­
Sample
Test
Statistic
3.0000
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Median
One­
Way
Analysis
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Data
Evaluation
Report
on
the
Reconnaissance
Survey
of
South
Florida
Amphibians
for
the
Assessment
of
Potential
Atrazine
Effects
EPA
MRID
Number
458677­
06
Page
54
of
54
NONPARAMETRIC
COMPARISON
OF
VITELLOGENIN
(
P_
mg)
BETWEEN
SITES
126
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
8
117.0
100.0
16.329932
14.6250
CP
8
138.0
100.0
16.329932
17.2500
UM
8
45.0
100.0
16.329932
5.6250
Kruskal­
Wallis
Test
Chi­
Square
11.8950
DF
2
Pr
>
Chi­
Square
0.0026
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
P_
mg
Classified
by
Variable
site
Sum
of
Expected
Std
Dev
Mean
site
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
BG
8
5.0
4.0
1.179536
0.6250
CP
8
7.0
4.0
1.179536
0.8750
UM
8
0.0
4.0
1.179536
0.0000
Median
One­
Way
Analysis
Chi­
Square
12.4583
DF
2
Pr
>
Chi­
Square
0.0020
