Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Data
Requirement:
EPA
DP
Barcode
D288775
EPA
MRID
458677­
11
EPA
Guideline
70­
1(
Special
Study)

Test
material:
Purity:
not
reported
Common
name:
Atrazine
Chemical
name:
IUPAC
CAS
name
6­
chloro­
N­
ethyl­
N'­(
1­
methylethyl)­
1,3,5­
triazine­
2,4­
diamine
CAS
No.
1912­
24­
9
Synonyms
EPA
PC
Code:
80803
Primary
Reviewer:
Thomas
M.
Steeger,
Ph.
D.,
Senior
Biologist
Date:
April
6,
2003
Environmental
Fate
and
Effects
Division,
ERB
4,
U.
S.
Environmental
Protection
Agency
Secondary
Reviewer(
s):
Joseph
E.
Tietge,
M.
S.,
Research
Aquatic
Biologist
Date:
Mid­
Continent
Ecology
Division,
National
Health
and
Environmental
Effects
Research
Laboratory
(
Duluth),
U.
S.
Environmental
Protection
Agency
Stephanie
Irene,
Ph.
D.,
Senior
Advisor
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
Mary
J.
Frankenberry,
Senior
Statistician
Date:
Environmental
Fate
and
Effects
Division,
ERB
3,
U.
S.
Environmental
Protection
Agency
EPA
PC
Code
080803
Date
Evaluation
Completed:
06/
01/
2003
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
2
of
70
CITATION:
Du
Preez,
L.,
K.
R.
Solomon
and
A.
M.
2003.
Exposure
of
Xenopus
laevis
larvae
to
different
concentrations
of
atrazine
in
semi­
natural
microcosms.
School
of
Environmental
Sciences
and
Development,
Zoology
Department,
Potchefstroom
University
of
CHE,
Potchefstroom
2520,
South
Africa.
Sponsor:
Syngenta
Crop
Protection,
Inc.,
Laboratory
Study
ID
ECORISK
Number
SA­
01­
D.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
3
of
70
EXECUTIVE
SUMMARY:

This
is
an
interim
report
to
assess
the
effects
of
atrazine
on
gonadal
abnormalities
in
Xenopus
laevis
metamorphs
through
the
use
of
microcosm
studies.
X.
laevis
adults
were
collected
from
an
earthen
pond
north
(
pond
C6)
of
Potchefstroom,
South
Africa,
based
on
the
reported
absence
of
atrazine
use
and
corn
production
in
its
watershed
and
the
presence
of
X.
laevis.
Adults
were
induced
to
spawn
in
the
laboratory
and
the
96­
hr
old
progeny
were
divided
among
4
treatments
(
0,
1,
10
and
25

g/
L
atrazine)
with
three
replicates
per
treatment
(
800
tadpoles
per
replicate).
Microcosms
consisted
of
1600­
L
cement
tanks,
lined
with
polyethylene
with
a
3­
cm
sand
bottom,
and
filled
with
1,100
L
of
tap
water.
Larvae
were
exposed
until
they
reached
NF
stage
66
(
forelimb
emergence
and
complete
tail
resorption);
the
study
was
terminated
after
133
days
of
exposure.
A
total
of
150
stage­
66
frogs
were
sampled
per
treatment.
Snout­
vent
length,
weight,
days
to
stage
66
(
metamorphosis)
were
recorded
and
animals
were
examined
for
gross
gonadal
morphology.

Mean
measured
concentrations
of
atrazine
ranged
from
0.9
to
1.82,
10
to
15.9
and
23.8
to
39.7

g/
L
for
the
1,
10
and
25

g/
L
nominal
treatments,
respectively.
Atrazine
in
reference
tanks
ranged
from
0
to
0.1

g/
L.
Although
some
animals
reached
stage
66
by
70
days,
the
majority
did
not
reach
metamorphosis
until
between
126
to
133
days.
The
study
authors
attributed
low
water
temperatures
to
the
delayed
development
of
the
tadpoles.
Based
on
gross
morphology,
the
incidence
of
gonadal
deformities
in
1,
10
and
25

g/
L
atrazine
groups
was
1.3,
0.7
and
3.3%
of
the
total
frogs
examined
(
150),
respectively;
reference
frogs
exhibited
a
4%
incidence
of
gonadal
deformities.
Discontinuous
testis
was
the
only
gonadal
abnormality
identified;
no
abnormalities
were
observed
in
the
ovaries
except
for
one
ovary
that
was
reduced
in
size.
Males
comprised
48%,
39%
and
47%
of
the
1.0,
10
and
25

g/
L
atrazine­
treated
samples,
respectively,
while
the
reference
samples
was
45%
male.

This
interim
microcosm
study
represents
a
reasonable
step
forward
in
testing
for
effects
observed
in
the
laboratory
and
may
provide
useful
information
when
all
the
analyses
are
completed.
The
present
study,
though,
showed
that
developmental
rates
across
all
treatments,
in
terms
of
time
to
metamorphosis,
were
delayed.
Fluctuating
water
quality
in
the
microcosm
units
may
have
impacted
the
developmental
rate.
Additionally,
the
study
was
designed
with
the
assumption
that
phytoplankton
growth
would
serve
as
a
source
of
food
for
developing
tadpoles.
Although
the
authors
note
that
phytoplankton
"
flourished,"
no
measure
(
e.
g.,
chlorophyl
a
concentration)
of
phytoplankton
growth
was
reported
and
supplemental
food
(
rabbit
pellets)
had
to
be
provided
to
the
developing
tadpoles.
However,
phytoplankton
growth
could
have
been
significantly
limited
by
atrazine
treatment,
thereby
resulting
in
indirect
effects
of
atrazine
on
development.
In
support
of
this
possibility,
atrazine­
treated
male
and
female
frogs
were
significantly
smaller
in
terms
of
length
and
weight
than
their
"
untreated"
counterparts,
further
complicating
interpretation
of
the
study
results.
This
assessment
was
based
on
gross
morphology
alone,
and
the
results
may
change
when
histology
is
completed.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
4
of
70
I.
MATERIALS
AND
METHODS
GUIDELINE
FOLLOWED:
Nonguideline
Study
COMPLIANCE:
Not
conducted
in
accordance
with
the
Good
Laboratory
Practices
as
outlined
in
40
CFR
Part
160,
August
19,
1989.
However,
GLP
elements
were
incorporated
into
study
conduct
including:

°
This
study
was
conducted
in
accordance
with
a
written
protocol,
signed
by
the
Study
Director
and
Principal
Investigator
°
Standard
Operating
Procedures
were
developed/
available
for
official
activities.
°
Protocol
amendments/
deviations
were
written
and
signed
by
the
Study
Director.

A.
MATERIALS:

1.
Test
Material
Atrazine
Description:
Not
reported
Lot
No./
Batch
No.
:
Not
reported
Purity:
Not
reported
Stability
of
compound
uder
Test
Conditions:
Not
reported
Storage
conditions
of
test
chemicals:
Not
reported
2.
Test
organism:

Species:
African
clawed
frog
(
Xenopus
laevis)
.
Age
at
test
initiation
:
96­
hr
larvae
Weight
at
study
initiation
(
mean
and
range):
Not
reported
Length
at
study
initiation
(
mean
and
range):
Not
reported
Source:
Adult
X.
laevis
obtained
from
earthen
pond
(
C6)
16
km
north
of
the
city
of
Potchefstroom,
South
Africa.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
5
of
70
B.
STUDY
DESIGN:

Objective:
1.
To
assess
the
effects
of
exposure
to
atrazine
on
gonadal
abnormalities
in
Xenopus
laevis
metamorphs
and
sub­
adults
under
semi­
natural
conditions
in
microcosms.

1.
Experimental
Conditions
a)
Range­
finding
Study:

b)
Definitive
Study
Table
1
.
Experimental
Parameters
Parameter
Details
Acclimation:
period:
Conditions:
(
same
as
test
or
not)
Feeding:
Health:
(
any
mortality
observed)
Microcosms
stabilized
for
5
months
Adults
paired
and
injected
with
chorionic
gonadotropin
(
Pregnyl
®
)
to
induce
spawning
and
placed
in
300
x
240
x
240
mm)
spawning
tanks
at
30oC.
After
spawning,
adults
were
removed
from
tanks
and
water
was
oxygenated.

Duration
of
the
test
NF
Stage
66
(
forelimb
emergence
and
tail
resorbtion)
or
19
weeks
total
exposure
whichever
came
first.

Test
condition
static/
flow­
through
Type
of
dilution
system
for
flowthrough
method.

Renewal
rate
for
static
renewal
static
water
maintained
at
1,100
L
mark
by
addition
of
tap
water
when
necessary
Aeration,
if
any
not
reported
Test
vessel
Material:
(
glass/
stainless
steel)
Size:
Fill
volume:
Cement
with
polyethylene
liner
2.25
m
x
0.6
m
x
1.2
m
=
1.62
m3
(
1,620
L)
1,100
L
plastic
liner
covered
with
3­
cm
layer
of
sandy
soil
Source
of
dilution
water
Quality:
Tap
water
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Parameter
Details
Page
6
of
70
Water
parameters:
Hardness
pH
Dissolved
oxygen
Total
Organic
carbon
Particulate
Matter
Ammonia
Nitrite
Metals
Pesticides
Chlorine
Temperature
{
Salinity
for
marine
or
estuarine
species}

Intervals
of
water
quality
measurement
Measured
every
Thursday
mid­
morning.

Number
of
replicates/
groups:
negative
control:
water
treated
ones:
3
Number
of
organisms
per
replicate
/
groups:
800
(
from
two
separate
spawns:
600
from
on
Sept.
5th
and
200
from
Sept.
12th
)

Biomass
loading
rate
800
tadpoles
per
1,100
L
Test
concentrations:
nominal:
measured:
1,
10
and
25

g/
L
0.91
­
1.82

g/
L;
10
­
15.9

g/
L;
23.8
­
39.7

g/
L
Solvent
(
type,
percentage,
if
used)
none
Lighting
natural
sunlight
Feeding
supplemental
rabbit
food
(
Complete
Rabbit
Pellets);
20
grams
homogenized
in
500
ml
of
tap
water
provided
once
per
week
but
later
twice
per
week.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Parameter
Details
Page
7
of
70
Recovery
of
chemical
Level
of
Quantitation
Level
of
Detection
GC/
MS
used
to
measure
atrazine
0.1

g/
L
Positive
control
{
if
used,
indicate
the
chemical
and
concentrations}
None
Other
parameters,
if
any
2.
Observations:

Table
2:
Observations
Criteria
Details
Parameters
measured
including
the
sublethal
effects/
toxicity
symptoms
body
weight,
snout­
vent
length,
time
(
days)
to
metamorphosis;
gross
morphology
and
histology
of
gonads.

Observation
intervals
Every
14
days
1­
L
water
samples
collected
for
atrazine
measurement;
water
chemistry
weekly.
From
11/
5/
02
until
mid­
January
2003,
metamorphs
removed
from
microcosms
every
2nd
day.

Were
raw
data
included?
Yes
Other
observations,
if
any
Report
notes
that
800
tadpoles
per
microcosm
were
well
below
the
effective
density
of
1
tadpole/
L
for
a
frog
to
reach
metamorphosis
successfully
(
Weldon
1999).
Xenopus
bucket
traps
baited
with
marrow
bones
used
to
catch
metamorphs.

The
report
notes
that
150
metamorphs
were
collected
from
each
treatment.
The
researchers
attempted
to
collect
50
from
each
of
the
three
replicates;
however,
they
were
apparently
unable
to
do
this
in
all
cases
and
therefore
had
to
sample
more
from
one
replicate
than
the
others.
According
to
the
protocol,
no
more
than
75
metamorphs
would
be
collected
from
a
single
tank.

II.
RESULTS
and
DISCUSSION:
[
All
results
discussed
in
this
section
and
the
next
are
those
reported
by
the
study
authors.
Although
supplemental
data
are
typically
used
in
a
qualitative
manner
only,
EFED
verified
spreadsheet
data
and
ran
basic
statistical
analyses
on
the
major
study
parameters.
See
attached
appendix.
If
results
differed
in
any
substantive
way,
the
difference
was
reported
in
the
text
below.]
Atrazine
concentrations
were
relatively
consistent
with
nominal
levels.
On
one
occasion,
atrazine
was
detected
in
one
of
the
reference
microcosms
at
a
concentration
equal
to
the
method
detection
limit
(
0.1

g/
L).
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
8
of
70
Microcosms
were
colder
than
natural
water
bodies
(
no
data
provided).
Extensive
early
mortality
was
observed
in
microcosm
1
(
1

g/
L),
while
extensive
mortality
and
slow
development
was
observed
in
microcosm
8(
10

g/
L);
both
were
excluded
from
the
analysis.

First
metamorphs
reached
development
stage
66
in
the
10th
week.
Collection
of
metamorphs
terminated
in
week
19
when
the
target
number
(
150/
treatment)
was
reached.
The
majority
of
frogs
reaching
stage
66
appears
to
have
occurred
between
126
and
133
days
of
exposure.

All
600
specimens
(
150
frogs/
treatment
*
4
treatments)
were
examined
visually
and
under
a
dissecting
microscope
for
gonadal
deformities.
Of
the
total
number
of
frogs
exposed
to
1,
10
and
25

g/
L
of
atrazine,
1.33%,
0.67%
and
3.33%
exhibited
gonadal
deformities,
respectively,
compared
to
4%
in
reference
microcosms.
No
intersex
gonads
were
observed.
Discontinuous
testis
was
the
most
frequently
observed
anomaly
and
ranged
from
1.7%
to
7.1%
in
atrazine
­
treated
animals
and
8.8%
in
controls.
No
gonadal
abnormalities
were
detected
in
females
except
for
one
female
with
a
reduced­
size
ovary.
The
study
concludes
that
based
on
gross
morphology,
atrazine
had
no
adverse
effect
on
gonadal
development
at
mean
concentrations
ranging
from
1.4
to
30.8

g/
L
under
semi­
field
conditions.

Table
3.
Total
number
of
frogs
examined
and
percentage
of
gonadal
deformities.

Nominal
Concentration
(
measured)


g/
L
Total
Number
Percent
Gonadal
Abnormalities
Frogs
Collected
Males
Females
Gonadal
Abnormalitie
s
Total
Frogs
Males
0
(
0
­
0.1)
150
68
82
6
4.0
8.8
1
(
0.91
­
1.82)
150
72
78
2
1.3
2.8
10
(
10
­
15.9)
150
59
91
1
0.7
1.7
25
(
23.8
­
39.7)
150
70
80
5
3.3
7.1
C.
REPORTED
STATISTICS:
Little
to
no
statistics
are
reported
in
this
interim
report.

D.
VERIFICATION
OF
STATISTICAL
RESULTS:
Statistical
analyses
run
using
SAS
®
(
Statistical
Analysis
System,
Release
8.01,
Cary,
North
Carolina);
see
attached
output.

E.
STUDY
DEFICIENCIES:
The
purity
of
atrazine
used
to
make
up
exposure
solutions
was
not
reported.
The
length
of
exposure
for
test
animals
differed;
one
group
consisting
of
600
were
exposed
for
approximately
one
week
longer
than
the
complement
of
200
additional
animals
added
later.
Tap
water
was
used
as
the
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
9
of
70
source
of
dilution
water.
Dissolved
oxygen
in
several
of
the
microcosms
dropped
below
3
mg/
L.
Exposure
tanks
were
not
all
at
the
same
temperature,
but
were
dependent
on
the
amount
of
shade
from
a
nearby
tree.
The
number
of
animals
sampled
from
each
replicate
may
have
been
different;
a
total
of
150
were
sampled
from
each
treatment,
but
up
to
75
may
have
been
sampled
from
a
replicate
rather
than
50
from
each
replicate.
Atrazine
was
detected
at
0.1

g/
L
(
detection
limit
of
method)
in
a
reference
tank
(
microcosm
3)

F.
REVIEWER'S
COMMENTS:

Frogs
were
collected
from
site
C6
because
of
the
absence
of
atrazine
use
in
the
watershed;
however,
this
site
was
characterized
in
previous
studies
(
Smith
et
al.
2003)
as
having
measurable
atrazine
concentrations
averaging
0.15

g/
L.
Although
this
concentration
is
relatively
low,
it
does
represent
a
level
that
has
been
reported
to
cause
gonadal
deformities
in
males
(
Hayes
et
al.
2002).
The
potential
for
gonadal
deformities
in
the
control
limits
the
usefulness
of
this
study
in
testing
the
hypothesis.
.

The
conditions
under
which
the
tests
were
conducted
probably
resulted
in
poor
water
quality,
which
contributed
to
the
relatively
retarded
development,
although
low
temperatures
in
the
initial
few
weeks
of
the
study
were
also
a
factor.
However,
the
exposure
media
was
never
changed
and
the
total
volume
was
not
replenished
to
make
up
for
evaporative
losses.
This
approach
would
lead
to
the
accumulation
of
waste
products,
especially
since
the
study
was
run
for
19
weeks.
Unfortunately,
mortality
was
not
evaluated
throughout
the
study,
so
it
is
difficult
to
assess
the
appropriateness
of
the
exposure
protocol.
All
that
one
can
tell
is
that
a
maximum
of
75
organisms
were
sampled
from
any
one
tank,
representing
less
than
10%
of
the
initial
set.
It
is
unclear
why
the
study
started
out
with
so
many
organisms
in
each
tank.

Microcosms
1
and
8
reportedly
exhibited
extensive
mortality
early
in
the
study
and
the
authors
alluded
to
possible
temperature
shock
because
microcosm
1
was
shaded;
however,
several
of
the
microcosms
were
shaded
by
trees
and
it
is
unclear
why
only
one
would
have
had
such
excessively
low
temperature.
Also,
temperature
shock
usually
results
from
too
high
a
temperature,
not
too
low
a
temperature.
Microcosm
8
exhibited
slow
metamorphosis
and
the
frogs
were
not
at
stage
66
by
133
days.
Although
microcosms
were
excluded
from
the
stud,
the
cause
of
mortality
and
delayed
development
was
not
adequately
characterized.

The
method
detection
limit
(
0.1

g/
L)
seems
unusually
high
for
GC/
MS
given
that
ELISA
assays
have
a
detection
limit
of
0.025

g/
L.

Frogs
were
relatively
slow
to
reach
metamorphosis
stage
66.
According
to
the
authors,
under
controlled
laboratory
conditions
of
20
­
25oC,
X.
laevis
tadpoles
take
58
days
to
complete
metamorphosis
(
Nieuwkoop
and
Faber
1967)
and
under
natural
conditions
where
water
temperatures
may
fluctuate
widely,
metamorphosis
may
take
from
56
to
63
days,
still
a
shorter
period
of
time
than
the
minimal
70
days
required
in
the
current
study.

Because
the
test
animals
were
added
in
two
stages,
exposure
times
differed.
Animals
(
600)
added
to
the
tank
first
were
exposed
for
roughly
a
week
longer
than
the
second
complement
(
200)
of
animals
added
to
bring
the
total
number
of
tadpoles
per
microcosm
to
800.

Although
the
first
metamorphs
reached
stage
66
by
70
days,
the
majority
of
study
animals
did
not
reach
stage
66
until
126
to
133
days,
which
implies
that
the
study
conditions
delayed
development
and
may
have
rendered
the
animals
less
sensitive
to
any
developmental
effects
of
atrazine
should
they
exist.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
10
of
70
Although
raw
water
quality
data
were
not
provided,
a
series
of
figures
depicted
water
temperature,
pH,
dissolved
oxygen
(
DO)
and
conductivity
over
the
study
period.
It
appears
from
these
graphs
that
something
(
perhaps
a
high
rain
event)
happened
around
Day
90
to
100
that
caused
conductivity,
temperature
and
pH
to
drop
substantially.
Conductivity
in
all
but
three
of
the
exposure
tanks
tended
to
steadily
increase
from
days
0
to
90;
however,
tanks
2,4,
6
and
9
had
very
erratic
conductivity
with
steep
declines.
In
tanks
4
and
6,
conductivity
plummeted
Day
40
and
in
tank
2
conductivity
plummeted
by
day
60.

By
Day
100,
dissolved
oxygen
in
some
replicates
of
the
reference,
10
and
25

g/
L
treatments
dropped
below
2
mg/
L.
Dissolved
oxygen
and
conductivity
in
the
25

g/
L
treatment
were
unusually
variable
relative
to
the
other
treatments.
It
is
possible
that
the
supplemental
feeding
resulted
in
food
residues
that
increased
conductivity
during
days
0
through
90
and
that
a
high
rain
event
may
have
resulted
in
a
rapid
influx
of
water
that
lowered
conductivity
and
dissolved
oxygen.
The
low
oxygen
content
of
the
water
is
difficult
to
explain
because
water
samples
were
collected
mid­
day
when
photosynthesis
by
phytoplankton
should
supplement
dissolved
oxygen
levels.
Heavy
rain
charged
with
carbon
dioxide
could
have
lowered
DO;
however,
it
should
have
also
altered
pH
which
does
not
appear
to
have
occurred.
However,
given
that
the
report
states
that
very
little
rain
fell
during
the
study,
it
is
uncertain
why
water
quality
changed
so
dramatically.

The
study
was
intentionally
designed
to
keep
the
loading
rate
below
1
tadpole
per
liter.
Because
the
reference
was
from
1999,
it
suggests
that
the
authors
were
aware
that
higher
loading
rates
would
have
compromised
the
development
of
tadpoles.

Total
time
to
metamorphosis
cannot
be
determined
from
this
study
because
an
unknown
number
of
animals
were
sacrificed
by
Day
133.
It
is
likely
that
tadpoles
could
have
died
and
not
been
accounted
for
during
the
course
of
the
study.
What
is
apparent,
though,
is
that
metamorphosis
was
considerably
delayed
for
the
majority
of
animals
in
this
study.

Although
no
intersex
gonads
were
observed,
the
current
analysis
is
based
on
gross
morphology
alone
and
does
not
represent
an
analysis
based
on
histology.

Although
the
stated
objectives
were
to
analyze
snout­
vent
length
(
SVL)
and
mass,
the
report
did
not
present
an
analysis
of
these
data.
In
the
table
below,
the
mean
SVL
and
mean
mass
for
each
treatment
replicate
were
extracted
from
the
appendix.
No
statistical
analysis
was
done
on
this
data
set,
but
it
appears
as
if
there
may
be
significant
reductions
in
SVL
and
mass
associated
with
atrazine
exposure.
EFED's
analysis
(
see
attached
output)
revealed
that
on
average,
male
and
female
frogs
not
exposed
to
atrazine
were
longer
21.17
mm
and
22.07
mm,
respectively,
than
frogs
from
atrazine­
treated
groups
(
Table
4);
non­
exposed
males
and
females
also
tended
to
weigh
more,
i.
e.,
1.08
and
1.20
g,
respectively,
than
frogs
treated
with
atrazine.
Analysis
of
variance
(
see
attached
printout)
revealed
that
both
males
and
females
were
significantly
larger
in
terms
of
weight
(
p
<
0.02)
and
length
(
p<
0.03)
than
atrazine
treated
males
and
females.
It
is
unclear
whether
the
difference
in
size
is
due
to
the
direct
effects
of
atrazine
on
growth
or
whether
it
is
due
to
reduced
phytoplankton
caused
by
atrazine
treatment.
At
each
treatment
though,
there
was
no
difference
in
weight
or
length
of
males
versus
females.

Table
4.
Summary
of
average
male
and
female
Xenopus
laevis
lengths
and
weights
with
associated
standard
deviations
by
atrazine
concentration.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
11
of
70
Atrazine
Concentration
Sex
Length
Standard
Deviation
Weight
Standard
Deviation
0
Female
21.17
3.80
1.08
0.75
0
Male
22.07
3.97
1.20
0.78
1
Female
18.55
2.70
0.70
0.32
1
Male
18.78
2.36
0.71
0.28
10
Female
18.82
3.36
0.73
0.51
10
Male
18.84
3.17
0.75
0.61
25
Female
17.50
3.11
0.59
0.40
25
Male
18.17
2.79
0.64
0.34
Similarly,
there
was
no
analysis
on
time
to
metamorphosis,
as
originally
stated
in
the
objective
section
of
the
study.
However,
given
the
method
by
which
metamorphs
were
removed,
and
given
the
confounding
effects
of
the
differential
exposure
during
the
first
week
of
the
study,
this
analysis
may
not
be
possible.
Individual
treatment
data
for
each
tank
is
needed
for
Figure
2.
Clearly,
the
data
were
collected
for
each
tank,
but
for
some
reason
they
were
not
presented
in
this
study.
Also,
some
very
large
organisms
were
collected
that
were
up
to
4
to
6
times
the
mean
weight
of
the
entire
sample,
suggesting
that
these
organisms
were
not
collected
at
completion
of
metamorphosis,
but
some
time
much
later.

In
terms
of
the
gonadal
analysis,
only
the
gonads
of
the
first
150
stage
66
organisms
in
each
treatment
were
evaluated.
This
sampling
would
bias
the
evaluation
toward
the
faster
developing
organisms,
which
is
not
representative
of
the
population
being
tested.
Only
150
organisms
were
sampled,
representing
6.25%
of
the
original
organisms
in
a
treatment.

H.
REFERENCES:

Hayes,
T.
B.,
A.
Collins,
M.
Lee,
M.
Mendoza,
N.
Noriega,
A.
S.
Stuart,
and
A.
Vonk.
2002a.
Hermaphroditic,
demasculinized
frogs
after
exposure
to
the
herbicide
atrazine
at
low
ecologically
relevant
doses.
Proceedings
of
the
National
Academy
of
Sciences
of
the
United
States
of
America
99(
8):
5476
­
5480
Nieuwkoop,
P.
D.
and
J.
Faber.
1967.
Normal
table
of
Xenopus
laevis
(
Daudin).
North­
Holland
Publishing
Company,
Amsterdam.

Smith,
E.
E.,
L.
Du
Preez,
and
K.
Solomon.
2003.
Gonadal
and
laryngeal
responses
to
field
exposure
of
Xenopus
laevis
to
atrazine
in
areas
of
corn
production
in
South
Africa.
The
Institute
of
Environmental
and
Human
Health,
Texas
Tech
University,
Lubbock,
TX
and
School
of
Environmental
Sciences
and
Development,
Potchefstroom
University
for
CHE,
Potchefstroom,
South
Africa.
Sponsor:
Syngenta
Crop
Protection,
Inc.,
Laboratory
Study
ID:
ECORISK
Number
SA­
01C.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
12
of
70
Weldon,
C.
1999.
The
sustainable
utilization
of
the
African
clawed
frog
Xenopus
laevis
(
Daudin).
M.
Sc.
Thesis.
Department
of
Zoology
and
Entomology,
University
of
the
Orange
Free
State,
Bloemfontein,
South
Africa.
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
13
of
70
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
14
of
70
MEAN
LENGTH
AND
WEIGHT
FOR
ALL
FROGS
COLLECTED
AT
EACH
SITE
621
Obs
ATRAZINE
SITE
_
TYPE_
_
FREQ_
LENGTH
WEIGHT
L_
SD
W_
SD
1
0
12
0
61
21.3213
1.09951
4.09761
0.76949
2
0
3
0
67
21.5955
1.10149
3.49729
0.67835
3
0
7
0
22
22.2409
1.34364
4.50816
0.96341
4
1
11
0
75
17.5907
0.57760
1.82380
0.18347
5
1
4
0
75
19.7347
0.83400
2.70368
0.34261
6
10
10
0
75
18.1227
0.61533
1.63381
0.20565
7
10
5
0
75
19.5400
0.86227
4.22665
0.73003
8
25
2
0
75
18.4040
0.66387
2.59393
0.37961
9
25
6
0
47
17.5574
0.60936
3.52807
0.39261
10
25
9
0
28
16.5679
0.48536
2.52866
0.28908
MEAN
LENGTH
AND
WEIGHT
FOR
MALE
AND
FEMALE
FROGS
COLLECTED
AT
EACH
SITE
622
Obs
ATRAZINE
SITE
Sex
_
TYPE_
_
FREQ_
LENGTH
WEIGHT
L_
SD
W_
SD
1
0
12
F
0
29
20.5931
1.03276
4.17312
0.81263
2
0
12
M
0
32
21.9813
1.16000
3.97772
0.73595
3
0
3
F
0
41
21.5585
1.07951
3.24700
0.59480
4
0
3
M
0
26
21.6538
1.13615
3.92624
0.80419
5
0
7
F
0
12
21.2417
1.19250
4.70483
1.07726
6
0
7
M
0
10
23.4400
1.52500
4.17591
0.82481
7
1
11
F
0
39
17.4103
0.56821
1.99272
0.18953
8
1
11
M
0
36
17.7861
0.58778
1.62659
0.17879
9
1
4
F
0
39
19.6974
0.84026
2.85671
0.36757
10
1
4
M
0
36
19.7750
0.82722
2.56742
0.31844
11
10
10
F
0
47
18.0426
0.61362
1.66417
0.21999
12
10
10
M
0
28
18.2571
0.61821
1.60241
0.18286
13
10
5
F
0
43
19.6791
0.86488
4.40317
0.68274
14
10
5
M
0
32
19.3531
0.85875
4.03908
0.80039
15
25
2
F
0
41
18.4561
0.68610
2.88037
0.44866
16
25
2
M
0
34
18.3412
0.63706
2.24150
0.27891
17
25
6
F
0
21
17.1619
0.57143
3.59256
0.39082
18
25
6
M
0
26
17.8769
0.64000
3.51287
0.39905
19
25
9
F
0
19
15.8316
0.41368
2.22862
0.18860
20
25
9
M
0
9
18.1222
0.63667
2.52971
0.40503
MEAN
LENGTH
AND
WEIGHT
FOR
MALE
AND
FEMALE
FROGS
BY
ATRAZINE
CONC
623
Obs
ATRAZINE
Sex
_
TYPE_
_
FREQ_
LENGTH
WEIGHT
L_
SD
W_
SD
1
0
F
0
82
21.1707
1.07951
3.79852
0.75050
2
0
M
0
68
22.0706
1.20456
3.97068
0.77549
3
1
F
0
78
18.5538
0.70423
2.70405
0.32116
4
1
M
0
72
18.7806
0.70750
2.35723
0.28334
5
10
F
0
90
18.8244
0.73367
3.35507
0.51080
6
10
M
0
60
18.8417
0.74650
3.17032
0.60543
7
25
F
0
81
17.5049
0.59247
3.10676
0.39879
8
25
M
0
69
18.1377
0.63812
2.78832
0.34000
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
15
of
70
PLOT
OF
FROG
WEIGHT
OVER
ATRAZINE
CONCENTRATION
FOR
MALE
AND
FEMALE
FROGS
624
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Plot
of
WEIGHT*
ATRAZINE.
Symbol
used
is
'*'.

1.1
ê
é
*
é
é
é
é
é
1.0
ê
é
é
é
é
é
é
0.9
ê
é
é
WEIGHT
é
é
é
é
0.8
ê
é
é
é
é
é
*
é
0.7
ê
*
é
é
é
é
é
é
0.6
ê
é
*
é
é
é
é
é
0.5
ê
èââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââ
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
ATRAZINE
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
16
of
70
PLOT
OF
FROG
WEIGHT
OVER
ATRAZINE
CONCENTRATION
FOR
MALE
AND
FEMALE
FROGS
625
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Plot
of
WEIGHT*
ATRAZINE.
Symbol
used
is
'*'.

1.2
ê
*
é
é
é
é
é
é
1.1
ê
é
é
é
é
é
é
1.0
ê
é
é
WEIGHT
é
é
é
é
0.9
ê
é
é
é
é
é
é
0.8
ê
é
é
é
é
*
é
é
*
0.7
ê
é
é
é
é
*
é
é
0.6
ê
èââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââ
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
ATRAZINE
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
17
of
70
ANALYSIS
OF
VARIANCE
FOR
FROG
WEIGHT
BETWEEN
TREATMENT
GROUPS
BY
SEX
626
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
GLM
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
331
Dependent
Variable:
MASS
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
10.8440965
3.6146988
13.21
<.
0001
Error
327
89.5108805
0.2737336
Corrected
Total
330
100.3549770
R­
Square
Coeff
Var
Root
MSE
MASS
Mean
0.108057
67.26132
0.523196
0.777855
Source
DF
Type
I
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
10.84409653
3.61469884
13.21
<.
0001
Source
DF
Type
III
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
10.84409653
3.61469884
13.21
<.
0001
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
18
of
70
ANALYSIS
OF
VARIANCE
FOR
FROG
WEIGHT
BETWEEN
TREATMENT
GROUPS
BY
SEX
628
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
GLM
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
269
Dependent
Variable:
MASS
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
13.56982643
4.52327548
15.88
<.
0001
Error
265
75.47985684
0.28482965
Corrected
Total
268
89.04968327
R­
Square
Coeff
Var
Root
MSE
MASS
Mean
0.152385
64.76464
0.533694
0.824052
Source
DF
Type
I
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
13.56982643
4.52327548
15.88
<.
0001
Source
DF
Type
III
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
13.56982643
4.52327548
15.88
<.
0001
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
19
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
WEIGHT
BY
SEX
630
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Moments
N
331
Sum
Weights
331
Mean
0
Sum
Observations
0
Std
Deviation
0.52081195
Variance
0.27124509
Skewness
3.47817499
Kurtosis
16.8585986
Uncorrected
SS
89.5108805
Corrected
SS
89.5108805
Coeff
Variation
.
Std
Error
Mean
0.02862641
Basic
Statistical
Measures
Location
Variability
Mean
0.00000
Std
Deviation
0.52081
Median
­
0.12423
Variance
0.27125
Mode
­
0.24951
Range
4.39000
Interquartile
Range
0.29944
Tests
for
Location:
Mu0=
0
Test
­
Statistic­
­­­­­
p
Value­­­­­­

Student's
t
t
0
Pr
>
|
t|
1.0000
Sign
M
­
60.5
Pr
>=
|
M|
<.
0001
Signed
Rank
S
­
9276
Pr
>=
|
S|
<.
0001
Tests
for
Normality
Test
­­
Statistic­­­
­­­­­
p
Value­­­­­­

Shapiro­
Wilk
W
0.670492
Pr
<
W
<
0.0001
Kolmogorov­
Smirnov
D
0.218809
Pr
>
D
<
0.0100
Cramer­
von
Mises
W­
Sq
5.537051
Pr
>
W­
Sq
<
0.0050
Anderson­
Darling
A­
Sq
29.21036
Pr
>
A­
Sq
<
0.0050
Quantiles
(
Definition
5)

Quantile
Estimate
100%
Max
3.6004878
99%
2.4504878
95%
0.9657692
90%
0.4175309
75%
Q3
0.0557692
50%
Median
­
0.1242308
25%
Q1
­
0.2436667
10%
­
0.3495122
5%
­
0.4436667
1%
­
0.7195122
0%
Min
­
0.7895122
Extreme
Observations
­­­­­­
Lowest­­­­­­
­­­­­
Highest­­­­­

Value
Obs
Value
Obs
­
0.789512
1
2.18753
279
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
20
of
70
­
0.739512
38
2.45049
31
­
0.739512
6
2.92633
250
­
0.719512
42
3.38049
71
­
0.669512
7
3.60049
13
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
WEIGHT
BY
SEX
632
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Histogram
#
Boxplot
3.7+*
1
*
.
.*
1
*
.
.*
1
*
.
.*
1
*
.
.*
1
*
.
.*
1
*
1.5+*
3
*
.*
2
*
.**
4
*
.***
9
0
.**
4
0
.***
7
0
.******
18
|
.******************
52
+­­+­­+
.**************************************
114
*­­­­­*
.*******************************
92
+­­­­­+
.*****
13
|
­
0.7+***
7
0
­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­
*
may
represent
up
to
3
counts
The
UNIVARIATE
Procedure
Variable:
Resid
Normal
Probability
Plot
3.7+
*
|
|
*
|
|
*
|
|
*
|
|
*
|
|
*
1.5+
***
|
*
++
|
**
++++
|
***++
|
++*+
|
++++
**
|
++++
***
|
++++
******
|
**********
|
************
|
*****
++++
­
0.7+*
***
++++
+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
21
of
70
­
2
­
1
0
+
1
+
2
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
22
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
WEIGHT
BY
SEX
634
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Moments
N
269
Sum
Weights
269
Mean
0
Sum
Observations
0
Std
Deviation
0.53069884
Variance
0.28164126
Skewness
3.56627975
Kurtosis
19.3195663
Uncorrected
SS
75.4798568
Corrected
SS
75.4798568
Coeff
Variation
.
Std
Error
Mean
0.03235728
Basic
Statistical
Measures
Location
Variability
Mean
0.00000
Std
Deviation
0.53070
Median
­
0.12650
Variance
0.28164
Mode
­
0.18650
Range
4.69806
Interquartile
Range
0.36900
NOTE:
The
mode
displayed
is
the
smallest
of
4
modes
with
a
count
of
4.

Tests
for
Location:
Mu0=
0
Test
­
Statistic­
­­­­­
p
Value­­­­­­

Student's
t
t
0
Pr
>
|
t|
1.0000
Sign
M
­
40.5
Pr
>=
|
M|
<.
0001
Signed
Rank
S
­
4884.5
Pr
>=
|
S|
0.0001
Tests
for
Normality
Test
­­
Statistic­­­
­­­­­
p
Value­­­­­­

Shapiro­
Wilk
W
0.698635
Pr
<
W
<
0.0001
Kolmogorov­
Smirnov
D
0.177585
Pr
>
D
<
0.0100
Cramer­
von
Mises
W­
Sq
3.346828
Pr
>
W­
Sq
<
0.0050
Anderson­
Darling
A­
Sq
18.12
Pr
>
A­
Sq
<
0.0050
Quantiles
(
Definition
5)

Quantile
Estimate
100%
Max
3.863500
99%
3.115441
95%
0.885441
90%
0.491884
75%
Q3
0.112500
50%
Median
­
0.126500
25%
Q1
­
0.256500
10%
­
0.384559
5%
­
0.474559
1%
­
0.784559
0%
Min
­
0.834559
Extreme
Observations
­­­­­­
Lowest­­­­­­
­­­­­
Highest­­­­­

Value
Obs
Value
Obs
­
0.834559
39
1.34544
8
­
0.784559
27
2.14544
62
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
23
of
70
­
0.784559
24
3.11544
44
­
0.774559
63
3.19544
11
­
0.764559
5
3.86350
196
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
WEIGHT
BY
SEX
636
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Histogram
#
Boxplot
3.9+*
1
*
.
.
.
.*
2
*
.
.
.
.
.*
1
*
.
.
1.5+
.*
2
*
.***
5
0
.**
4
0
.****
8
0
.****
8
|
.***********
21
|
.*********************
42
+­­+­­+
.*****************************************
82
*­­­­­*
.*************************************
73
+­­­­­+
.******
12
|
.****
7
|
­
0.9+*
1
0
­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­
*
may
represent
up
to
2
counts
The
UNIVARIATE
Procedure
Variable:
Resid
Normal
Probability
Plot
3.9+
*
|
|
|
|
*
*
|
|
|
|
|
*
|
|
1.5+
|
**
+++
|
***++
|
**+
|
++***
|
++++***
|
++++
****
|
++++******
|
*********
|
***********
|
*****
++++
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
24
of
70
|*
*****
++++
­
0.9+*
+++
+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+
­
2
­
1
0
+
1
+
2
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
25
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
WEIGHT
BY
SEX
638
The
UNIVARIATE
Procedure
Variable:
Resid
Schematic
Plots
|
4
+
|
*
|
|
*
3.5
+
|
*
|
*
|
*
3
+
|
*
|
|
2.5
+
*
|
|
*
|
*
2
+
|
|
|
*
1.5
+
*
|
*
*
|
*
*
|
*
0
1
+
0
0
|
0
0
|
0
0
|
0
0
0.5
+
|
|
|
|
|
|
|
|
|
|
+­­­­­+
0
+
+­­+­­+
|
+
|
|
*­­­­­*
*­­­­­*
|
+­­­­­+
+­­­­­+
|
|
|
­
0.5
+
|
|
|
|
|
|
0
|
|
0
­
1
+
­­­­­­­­­­­­+­­­­­­­­­­­+­­­­­­­­­­­
Sex
F
M
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
26
of
70
REGRESSION
ANALYSIS
OF
WEIGHT
OVER
ATRAZINE
CONCENTRATION
639
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
REG
Procedure
Model:
MODEL1
Dependent
Variable:
WEIGHT
Analysis
of
Variance
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Model
1
0.06563
0.06563
1.96
0.2969
Error
2
0.06711
0.03356
Corrected
Total
3
0.13274
Root
MSE
0.18318
R­
Square
0.4944
Dependent
Mean
0.77747
Adj
R­
Sq
0.2416
Coeff
Var
23.56145
Parameter
Estimates
Parameter
Standard
Variable
DF
Estimate
Error
t
Value
Pr
>
|
t|

Intercept
1
0.89246
0.12309
7.25
0.0185
ATRAZINE
1
­
0.01278
0.00914
­
1.40
0.2969
REGRESSION
ANALYSIS
OF
WEIGHT
OVER
ATRAZINE
CONCENTRATION
640
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
REG
Procedure
Model:
MODEL1
Dependent
Variable:
WEIGHT
Analysis
of
Variance
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Model
1
0.07648
0.07648
1.25
0.3800
Error
2
0.12248
0.06124
Corrected
Total
3
0.19896
Root
MSE
0.24747
R­
Square
0.3844
Dependent
Mean
0.82417
Adj
R­
Sq
0.0766
Coeff
Var
30.02629
Parameter
Estimates
Parameter
Standard
Variable
DF
Estimate
Error
t
Value
Pr
>
|
t|

Intercept
1
0.94830
0.16628
5.70
0.0294
ATRAZINE
1
­
0.01379
0.01234
­
1.12
0.3800
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
27
of
70
PLOT
OF
FROG
LENGTH
OVER
ATRAZINE
CONCENTRATION
FOR
MALE
AND
FEMALE
FROGS
641
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Plot
of
LENGTH*
ATRAZINE.
Symbol
used
is
'*'.

LENGTH
é
21.5
ê
é
é
é
*
é
21.0
ê
é
é
é
é
20.5
ê
é
é
é
é
20.0
ê
é
é
é
é
19.5
ê
é
é
é
é
19.0
ê
é
é
*
é
é
*
18.5
ê
é
é
é
é
18.0
ê
é
é
é
é
17.5
ê
*
é
èââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââ
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
ATRAZINE
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
28
of
70
PLOT
OF
FROG
LENGTH
OVER
ATRAZINE
CONCENTRATION
FOR
MALE
AND
FEMALE
FROGS
642
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

Plot
of
LENGTH*
ATRAZINE.
Symbol
used
is
'*'.

LENGTH
é
é
22.5
ê
é
é
é
*
22.0
ê
é
é
é
21.5
ê
é
é
é
21.0
ê
é
é
é
20.5
ê
é
é
é
20.0
ê
é
é
é
19.5
ê
é
é
é
19.0
ê
é
*
é
*
é
18.5
ê
é
é
é
*
18.0
ê
é
èââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââêââ
0
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
ATRAZINE
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
29
of
70
ANALYSIS
OF
VARIANCE
FOR
FROG
LENGTH
BETWEEN
TREATMENT
GROUPS
BY
SEX
643
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
GLM
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
331
Dependent
Variable:
SVL
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
585.622241
195.207414
18.21
<.
0001
Error
327
3505.727849
10.720880
Corrected
Total
330
4091.350091
R­
Square
Coeff
Var
Root
MSE
SVL
Mean
0.143137
17.21578
3.274276
19.01903
Source
DF
Type
I
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
585.6222415
195.2074138
18.21
<.
0001
Source
DF
Type
III
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
585.6222415
195.2074138
18.21
<.
0001
ANALYSIS
OF
VARIANCE
FOR
FROG
LENGTH
BETWEEN
TREATMENT
GROUPS
BY
SEX
645
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
GLM
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
269
Dependent
Variable:
SVL
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
640.258332
213.419444
21.98
<.
0001
Error
265
2572.541817
9.707705
Corrected
Total
268
3212.800149
R­
Square
Coeff
Var
Root
MSE
SVL
Mean
0.199284
16.01009
3.115719
19.46097
Source
DF
Type
I
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
640.2583321
213.4194440
21.98
<.
0001
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
30
of
70
Source
DF
Type
III
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
640.2583321
213.4194440
21.98
<.
0001
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
31
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
LENGTH
BY
SEX
647
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Moments
N
331
Sum
Weights
331
Mean
0
Sum
Observations
0
Std
Deviation
3.25935848
Variance
10.6234177
Skewness
1.6487435
Kurtosis
4.17321286
Uncorrected
SS
3505.72785
Corrected
SS
3505.72785
Coeff
Variation
.
Std
Error
Mean
0.17915054
Basic
Statistical
Measures
Location
Variability
Mean
0.00000
Std
Deviation
3.25936
Median
­
0.62444
Variance
10.62342
Mode
­
1.42444
Range
22.72940
Interquartile
Range
2.77060
NOTE:
The
mode
displayed
is
the
smallest
of
2
modes
with
a
count
of
6.

Tests
for
Location:
Mu0=
0
Test
­
Statistic­
­­­­­
p
Value­­­­­­

Student's
t
t
0
Pr
>
|
t|
1.0000
Sign
M
­
36.5
Pr
>=
|
M|
<.
0001
Signed
Rank
S
­
5490
Pr
>=
|
S|
0.0015
Tests
for
Normality
Test
­­
Statistic­­­
­­­­­
p
Value­­­­­­

Shapiro­
Wilk
W
0.871872
Pr
<
W
<
0.0001
Kolmogorov­
Smirnov
D
0.1515
Pr
>
D
<
0.0100
Cramer­
von
Mises
W­
Sq
2.170587
Pr
>
W­
Sq
<
0.0050
Anderson­
Darling
A­
Sq
12.12968
Pr
>
A­
Sq
<
0.0050
Quantiles
(
Definition
5)

Quantile
Estimate
100%
Max
15.775556
99%
11.395062
95%
6.729268
90%
3.646154
75%
Q3
0.946154
50%
Median
­
0.624444
25%
Q1
­
1.824444
10%
­
3.004938
5%
­
4.104938
1%
­
5.670732
0%
Min
­
6.953846
Extreme
Observations
­­­­­­
Lowest­­­­­
­­­­­
Highest­­­­­

Value
Obs
Value
Obs
­
6.95385
83
10.4756
248
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
32
of
70
­
6.17073
1
11.3951
279
­
5.87073
6
13.5293
71
­
5.67073
38
13.9293
13
­
5.57073
5
15.7756
250
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
33
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
LENGTH
BY
SEX
649
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Histogram
#
Boxplot
15.5+*
1
*
.
.*
2
*
.
.*
1
*
.*
2
*
.**
3
*
.*
2
0
.***
5
0
.***
6
0
.***
6
0
4.5+**
3
|
.*****
10
|
.********
15
|
.************
23
|
.*************************
50
+­­+­­+
.******************************
59
*­­­­­*
.************************************
71
+­­­­­+
.*******************
38
|
.********
16
|
.******
11
|
.***
5
|
­
6.5+*
2
0
­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­
*
may
represent
up
to
2
counts
The
UNIVARIATE
Procedure
Variable:
Resid
Normal
Probability
Plot
15.5+
*
|
|
**
|
|
*
|
**
|
***
|
*
+
|
**
+++
|
**
+++
|
**+++
4.5+
**+
|
++**
|
+++**
|
+++****
|
+++*****
|
+******
|
*******
|
******+
|
****+++
|
*****+++
|
****
+++
­
6.5+*
+++
+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+
­
2
­
1
0
+
1
+
2
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
34
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
LENGTH
BY
SEX
651
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Moments
N
269
Sum
Weights
269
Mean
0
Sum
Observations
0
Std
Deviation
3.09823121
Variance
9.59903663
Skewness
1.47756279
Kurtosis
4.18236889
Uncorrected
SS
2572.54182
Corrected
SS
2572.54182
Coeff
Variation
.
Std
Error
Mean
0.18890249
Basic
Statistical
Measures
Location
Variability
Mean
0.00000
Std
Deviation
3.09823
Median
­
0.57059
Variance
9.59904
Mode
­
1.68056
Range
22.72892
Interquartile
Range
2.85713
Tests
for
Location:
Mu0=
0
Test
­
Statistic­
­­­­­
p
Value­­­­­­

Student's
t
t
0
Pr
>
|
t|
1.0000
Sign
M
­
29.5
Pr
>=
|
M|
0.0004
Signed
Rank
S
­
2839.5
Pr
>=
|
S|
0.0259
Tests
for
Normality
Test
­­
Statistic­­­
­­­­­
p
Value­­­­­­

Shapiro­
Wilk
W
0.903548
Pr
<
W
<
0.0001
Kolmogorov­
Smirnov
D
0.137053
Pr
>
D
<
0.0100
Cramer­
von
Mises
W­
Sq
1.231739
Pr
>
W­
Sq
<
0.0050
Anderson­
Darling
A­
Sq
6.644115
Pr
>
A­
Sq
<
0.0050
Quantiles
(
Definition
5)

Quantile
Estimate
100%
Max
15.858333
99%
12.229412
95%
6.029412
90%
4.162319
75%
Q3
1.119444
50%
Median
­
0.570588
25%
Q1
­
1.737681
10%
­
3.080556
5%
­
3.880556
1%
­
5.870588
0%
Min
­
6.870588
Extreme
Observations
­­­­­­
Lowest­­­­­
­­­­­­
Highest­­­­­

Value
Obs
Value
Obs
­
6.87059
27
8.45833
183
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
35
of
70
­
6.27059
63
8.52941
62
­
5.87059
24
12.22941
44
­
5.77059
39
13.32941
11
­
5.37059
5
15.85833
196
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
36
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
LENGTH
BY
SEX
653
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
UNIVARIATE
Procedure
Variable:
Resid
Histogram
#
Boxplot
15.5+*
1
*
.
.*
1
*
.*
1
*
.
.
.
.**
4
0
.*
2
0
.***
5
0
.**
4
|
4.5+*****
10
|
.******
11
|
.******
12
|
.***********
21
+­­­­­+
.*****************
33
|
+
|
.**************************
51
*­­­­­*
.****************************
56
+­­­­­+
.***************
30
|
.********
16
|
.***
6
|
.**
3
|
­
6.5+*
2
0
­­­­+­­­­+­­­­+­­­­+­­­­+­­­
*
may
represent
up
to
2
counts
The
UNIVARIATE
Procedure
Variable:
Resid
Normal
Probability
Plot
15.5+
*
|
|
*
|
*
|
|
|
|
***
|
*
+++
|
***
+++
|
**+++
4.5+
***+
|
+**
|
++**
|
++****
|
+++****
|
+******
|
*******
|
******+
|
*****+++
|
***
++++
|
***
+++
­
6.5+*
+++
+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+­­­­+
­
2
­
1
0
+
1
+
2
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
37
of
70
PROC
UNIVARIATE
OUTPUT
FOR
RESIDUALS
FROM
GLM
PROCEDURE
USING
FROG
LENGTH
BY
SEX
655
The
UNIVARIATE
Procedure
Variable:
Resid
Schematic
Plots
|
17.5
+
|
|
|
*
*
15
+
|
|
*
|
*
12.5
+
*
|
|
*
|
*
10
+
*
|
*
|
0
0
|
0
0
7.5
+
0
0
|
0
|
0
0
|
0
|
5
+
0
|
|
|
|
|
|
|
|
|
|
2.5
+
|
|
|
|
|
|
+­­­­­+
+­­­­­+
|
|
|
|
|
0
+
|
+
|
|
+
|
|
*­­­­­*
*­­­­­*
|
|
|
|
|
|
+­­­­­+
+­­­­­+
­
2.5
+
|
|
|
|
|
|
|
|
|
|
|
­
5
+
|
|
|
|
|
|
0
0
|
0
0
­
7.5
+
­­­­­­­­­­­­+­­­­­­­­­­­+­­­­­­­­­­­
Sex
F
M
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
38
of
70
REGRESSION
ANALYSIS
OF
LENGTH
OVER
ATRAZINE
CONCENTRATION
656
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
REG
Procedure
Model:
MODEL1
Dependent
Variable:
LENGTH
Analysis
of
Variance
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Model
1
3.99283
3.99283
2.51
0.2541
Error
2
3.18361
1.59180
Corrected
Total
3
7.17643
Root
MSE
1.26167
R­
Square
0.5564
Dependent
Mean
19.01349
Adj
R­
Sq
0.3346
Coeff
Var
6.63564
Parameter
Estimates
Parameter
Standard
Variable
DF
Estimate
Error
t
Value
Pr
>
|
t|

Intercept
1
19.91044
0.84775
23.49
0.0018
ATRAZINE
1
­
0.09966
0.06293
­
1.58
0.2541
REGRESSION
ANALYSIS
OF
LENGTH
OVER
ATRAZINE
CONCENTRATION
657
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
REG
Procedure
Model:
MODEL1
Dependent
Variable:
LENGTH
Analysis
of
Variance
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Model
1
3.94737
3.94737
1.45
0.3522
Error
2
5.46029
2.73015
Corrected
Total
3
9.40766
Root
MSE
1.65232
R­
Square
0.4196
Dependent
Mean
19.45762
Adj
R­
Sq
0.1294
Coeff
Var
8.49187
Parameter
Estimates
Parameter
Standard
Variable
DF
Estimate
Error
t
Value
Pr
>
|
t|

Intercept
1
20.34946
1.11024
18.33
0.0030
ATRAZINE
1
­
0.09909
0.08241
­
1.20
0.3522
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
39
of
70
ANOVA
FOR
FROG
LENGTH
BETWEEN
TREATMENTS
658
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
10
Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
24.31994787
8.10664929
6.17
0.0290
Error
6
7.88332214
1.31388702
Corrected
Total
9
32.20327001
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.755201
6.043316
1.146249
18.96722
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
24.31994787
8.10664929
6.17
0.0290
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
ATRAZINE
1
1.4602
1.4602
2.90
0.1640
Error
4
2.0163
0.5041
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
ATRAZINE
3
1.6226
0.6543
Dunnett's
t
Tests
for
LENGTH
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
1.313887
Critical
Value
of
Dunnett's
t
3.12383
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
ATRAZINE
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
10
­
0
­
2.2703
­
5.5390
0.9984
1
­
0
­
2.5773
­
5.8460
0.6915
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
40
of
70
25
­
0
­
3.9812
­
6.9049
­
1.0576
***
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
41
of
70
ANOVA
FOR
FROG
LENGTH
BETWEEN
TREATMENTS
662
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
10
Dependent
Variable:
LENGTH
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
32.02748490
10.67582830
14.25
0.0039
Error
6
4.49480602
0.74913434
Corrected
Total
9
36.52229092
R­
Square
Coeff
Var
Root
MSE
LENGTH
Mean
0.876930
4.402765
0.865525
19.65868
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
32.02748490
10.67582830
14.25
0.0039
Levene's
Test
for
Homogeneity
of
LENGTH
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
ATRAZINE
1
0.4820
0.4820
3.52
0.1337
Error
4
0.5470
0.1368
Bartlett's
Test
for
Homogeneity
of
LENGTH
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
ATRAZINE
3
3.1456
0.3697
Dunnett's
t
Tests
for
LENGTH
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.749134
Critical
Value
of
Dunnett's
t
3.12383
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
ATRAZINE
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
10
­
0
­
3.5532
­
6.0214
­
1.0851
***
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
42
of
70
1
­
0
­
3.5778
­
6.0460
­
1.1096
***
25
­
0
­
4.2449
­
6.4525
­
2.0373
***
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
43
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
ACROSS
TREATMENTS
666
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
27.0
16.50
4.387482
9.000000
1
2
10.0
11.00
3.829708
5.000000
10
2
10.0
11.00
3.829708
5.000000
25
3
8.0
16.50
4.387482
2.666667
Kruskal­
Wallis
Test
Chi­
Square
6.7455
DF
3
Pr
>
Chi­
Square
0.0805
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
3.0
1.50
0.763763
1.00
1
2
1.0
1.00
0.666667
0.50
10
2
1.0
1.00
0.666667
0.50
25
3
0.0
1.50
0.763763
0.00
Median
One­
Way
Analysis
Chi­
Square
5.4000
DF
3
Pr
>
Chi­
Square
0.1447
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
44
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
ACROSS
TREATMENTS
668
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
LENGTH
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
27.0
16.50
4.387482
9.000000
1
2
8.0
11.00
3.829708
4.000000
10
2
10.0
11.00
3.829708
5.000000
25
3
10.0
16.50
4.387482
3.333333
Kruskal­
Wallis
Test
Chi­
Square
6.0909
DF
3
Pr
>
Chi­
Square
0.1073
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
LENGTH
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
3.0
1.50
0.763763
1.00
1
2
1.0
1.00
0.666667
0.50
10
2
1.0
1.00
0.666667
0.50
25
3
0.0
1.50
0.763763
0.00
Median
One­
Way
Analysis
Chi­
Square
5.4000
DF
3
Pr
>
Chi­
Square
0.1447
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
45
of
70
ANOVA
FOR
FROG
WEIGHT
BETWEEN
TREATMENTS
670
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
10
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
0.47376094
0.15792031
7.93
0.0165
Error
6
0.11947701
0.01991283
Corrected
Total
9
0.59323795
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.798602
17.94657
0.141113
0.786294
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
0.47376094
0.15792031
7.93
0.0165
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
ATRAZINE
1
0.000095
0.000095
1.45
0.2953
Error
4
0.000264
0.000066
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
ATRAZINE
3
0.9200
0.8206
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.019913
Critical
Value
of
Dunnett's
t
3.12383
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
ATRAZINE
Between
95%
Confidence
Comparison
Means
Limits
10
­
0
­
0.3623
­
0.7647
0.0401
1
­
0
­
0.3974
­
0.7998
0.0050
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
46
of
70
25
­
0
­
0.5445
­
0.9044
­
0.1846
***
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
47
of
70
ANOVA
FOR
FROG
WEIGHT
BETWEEN
TREATMENTS
674
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
ATRAZINE
4
0
1
10
25
Number
of
observations
10
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
3
0.73743515
0.24581172
9.66
0.0103
Error
6
0.15260050
0.02543342
Corrected
Total
9
0.89003565
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.828546
18.48632
0.159479
0.862684
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
ATRAZINE
3
0.73743515
0.24581172
9.66
0.0103
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
ATRAZINE
1
0.00150
0.00150
4.00
0.1161
Error
4
0.00150
0.000376
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
ATRAZINE
3
12.4660
0.0059
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
6
Error
Mean
Square
0.025433
Critical
Value
of
Dunnett's
t
3.12383
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
ATRAZINE
Between
95%
Confidence
Comparison
Means
Limits
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
48
of
70
10
­
0
­
0.5352
­
0.9900
­
0.0805
***
1
­
0
­
0.5662
­
1.0210
­
0.1114
***
25
­
0
­
0.6358
­
1.0426
­
0.2290
***
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
49
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
ACROSS
TREATMENTS
BY
SEX
678
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
F
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
27.0
16.50
4.387482
9.00
1
2
8.0
11.00
3.829708
4.00
10
2
11.0
11.00
3.829708
5.50
25
3
9.0
16.50
4.387482
3.00
Kruskal­
Wallis
Test
Chi­
Square
6.5455
DF
3
Pr
>
Chi­
Square
0.0879
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
3.0
1.50
0.763763
1.00
1
2
1.0
1.00
0.666667
0.50
10
2
1.0
1.00
0.666667
0.50
25
3
0.0
1.50
0.763763
0.00
Median
One­
Way
Analysis
Chi­
Square
5.4000
DF
3
Pr
>
Chi­
Square
0.1447
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
50
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
ACROSS
TREATMENTS
BY
SEX
680
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
Sex=
M
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
27.0
16.50
4.387482
9.00
1
2
7.0
11.00
3.829708
3.50
10
2
9.0
11.00
3.829708
4.50
25
3
12.0
16.50
4.387482
4.00
Kruskal­
Wallis
Test
Chi­
Square
5.8364
DF
3
Pr
>
Chi­
Square
0.1198
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
ATRAZINE
Sum
of
Expected
Std
Dev
Mean
ATRAZINE
N
Scores
Under
H0
Under
H0
Score
ââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
0
3
3.0
1.50
0.763763
1.00
1
2
1.0
1.00
0.666667
0.50
10
2
1.0
1.00
0.666667
0.50
25
3
0.0
1.50
0.763763
0.00
Median
One­
Way
Analysis
Chi­
Square
5.4000
DF
3
Pr
>
Chi­
Square
0.1447
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
51
of
70
ANOVA
FOR
WEIGHT
BETWEEN
SEXES
682
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
0
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
6
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.04444191
0.04444191
1.64
0.2697
Error
4
0.10848814
0.02712203
Corrected
Total
5
0.15293005
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.290603
13.86664
0.164688
1.187654
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.04444191
0.04444191
1.64
0.2697
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Sex
1
0.00111
0.00111
2.89
0.1646
Error
4
0.00153
0.000384
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Sex
1
1.3299
0.2488
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
4
Error
Mean
Square
0.027122
Critical
Value
of
Dunnett's
t
2.77630
Minimum
Significant
Difference
0.3733
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
Sex
Between
95%
Confidence
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
52
of
70
Comparison
Means
Limits
M
­
F
0.1721
­
0.2012
0.5454
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
53
of
70
ANOVA
FOR
WEIGHT
BETWEEN
SEXES
686
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
1
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
4
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00001069
0.00001069
0.00
0.9872
Error
2
0.06567277
0.03283639
Corrected
Total
3
0.06568346
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.000163
25.67177
0.181208
0.705865
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.00001069
0.00001069
0.00
0.9872
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Sex
0
0
.
.
.
Error
0
0
.

Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Sex
1
0.0108
0.9171
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
2
Error
Mean
Square
0.032836
Critical
Value
of
Dunnett's
t
4.30263
Minimum
Significant
Difference
0.7797
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
54
of
70
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
0.003269
­
0.776402
0.782940
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
55
of
70
ANOVA
FOR
WEIGHT
BETWEEN
SEXES
690
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
10
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
4
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00000059
0.00000059
0.00
0.9969
Error
2
0.06049619
0.03024810
Corrected
Total
3
0.06049678
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.000010
23.53874
0.173920
0.738866
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
5.9017464E­
7
5.9017464E­
7
0.00
0.9969
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Sex
0
0
.
.
.
Error
0
0
.

Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Sex
1
0.00127
0.9716
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
2
Error
Mean
Square
0.030248
Critical
Value
of
Dunnett's
t
4.30263
Minimum
Significant
Difference
0.7483
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
56
of
70
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
­
0.0007682
­
0.7490802
0.7475438
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
57
of
70
ANOVA
FOR
WEIGHT
BETWEEN
SEXES
694
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
25
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
6
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00980227
0.00980227
1.05
0.3639
Error
4
0.03742040
0.00935510
Corrected
Total
5
0.04722267
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.207575
16.18803
0.096722
0.597489
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.00980227
0.00980227
1.05
0.3639
Levene's
Test
for
Homogeneity
of
WEIGHT
Variance
ANOVA
of
Squared
Deviations
from
Group
Means
Sum
of
Mean
Source
DF
Squares
Square
F
Value
Pr
>
F
Sex
1
0.000233
0.000233
4.00
0.1162
Error
4
0.000233
0.000058
Bartlett's
Test
for
Homogeneity
of
WEIGHT
Variance
Source
DF
Chi­
Square
Pr
>
ChiSq
Sex
1
11.6016
0.0007
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
4
Error
Mean
Square
0.009355
Critical
Value
of
Dunnett's
t
2.77630
Minimum
Significant
Difference
0.2193
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
58
of
70
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
0.08084
­
0.13841
0.30009
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
59
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
698
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
0
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
8.0
10.50
2.291288
2.666667
M
3
13.0
10.50
2.291288
4.333333
Wilcoxon
Two­
Sample
Test
Statistic
8.0000
Normal
Approximation
Z
­
0.8729
One­
Sided
Pr
<
Z
0.1914
Two­
Sided
Pr
>
|
Z|
0.3827
t
Approximation
One­
Sided
Pr
<
Z
0.2113
Two­
Sided
Pr
>
|
Z|
0.4227
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
1.1905
DF
1
Pr
>
Chi­
Square
0.2752
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
1.0
1.50
0.670820
0.333333
M
3
2.0
1.50
0.670820
0.666667
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.7454
One­
Sided
Pr
<
Z
0.2280
Two­
Sided
Pr
>
|
Z|
0.4561
Median
One­
Way
Analysis
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
60
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
700
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
1
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
5.0
5.0
1.290994
2.50
M
2
5.0
5.0
1.290994
2.50
Wilcoxon
Two­
Sample
Test
Statistic
5.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
1.0
1.0
0.577350
0.50
M
2
1.0
1.0
0.577350
0.50
Median
Two­
Sample
Test
Statistic
1.0000
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Median
One­
Way
Analysis
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
61
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
702
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
10
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
5.0
5.0
1.290994
2.50
M
2
5.0
5.0
1.290994
2.50
Wilcoxon
Two­
Sample
Test
Statistic
5.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
1.0
1.0
0.577350
0.50
M
2
1.0
1.0
0.577350
0.50
Median
Two­
Sample
Test
Statistic
1.0000
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Median
One­
Way
Analysis
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
62
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
WEIGHT
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
704
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
25
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
9.0
10.50
2.291288
3.0
M
3
12.0
10.50
2.291288
4.0
Wilcoxon
Two­
Sample
Test
Statistic
9.0000
Normal
Approximation
Z
­
0.4364
One­
Sided
Pr
<
Z
0.3313
Two­
Sided
Pr
>
|
Z|
0.6625
t
Approximation
One­
Sided
Pr
<
Z
0.3404
Two­
Sided
Pr
>
|
Z|
0.6807
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.4286
DF
1
Pr
>
Chi­
Square
0.5127
The
NPAR1WAY
Procedure
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
1.0
1.50
0.670820
0.333333
M
3
2.0
1.50
0.670820
0.666667
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.7454
One­
Sided
Pr
<
Z
0.2280
Two­
Sided
Pr
>
|
Z|
0.4561
Median
One­
Way
Analysis
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
63
of
70
ANOVA
FOR
LENGTH
BETWEEN
SEXES
706
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
0
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
6
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.04444191
0.04444191
1.64
0.2697
Error
4
0.10848814
0.02712203
Corrected
Total
5
0.15293005
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.290603
13.86664
0.164688
1.187654
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.04444191
0.04444191
1.64
0.2697
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
4
Error
Mean
Square
0.027122
Critical
Value
of
Dunnett's
t
2.77630
Minimum
Significant
Difference
0.3733
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Simultaneous
Sex
Between
95%
Confidence
Comparison
Means
Limits
M
­
F
0.1721
­
0.2012
0.5454
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
64
of
70
ANOVA
FOR
LENGTH
BETWEEN
SEXES
709
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
1
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
4
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00001069
0.00001069
0.00
0.9872
Error
2
0.06567277
0.03283639
Corrected
Total
3
0.06568346
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.000163
25.67177
0.181208
0.705865
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.00001069
0.00001069
0.00
0.9872
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
2
Error
Mean
Square
0.032836
Critical
Value
of
Dunnett's
t
4.30263
Minimum
Significant
Difference
0.7797
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
0.003269
­
0.776402
0.782940
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
65
of
70
ANOVA
FOR
LENGTH
BETWEEN
SEXES
712
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
10
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
4
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00000059
0.00000059
0.00
0.9969
Error
2
0.06049619
0.03024810
Corrected
Total
3
0.06049678
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.000010
23.53874
0.173920
0.738866
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
5.9017464E­
7
5.9017464E­
7
0.00
0.9969
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
2
Error
Mean
Square
0.030248
Critical
Value
of
Dunnett's
t
4.30263
Minimum
Significant
Difference
0.7483
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
­
0.0007682
­
0.7490802
0.7475438
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
66
of
70
ANOVA
FOR
LENGTH
BETWEEN
SEXES
715
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
25
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
ANOVA
Procedure
Class
Level
Information
Class
Levels
Values
Sex
2
F
M
Number
of
observations
6
Dependent
Variable:
WEIGHT
Sum
of
Source
DF
Squares
Mean
Square
F
Value
Pr
>
F
Model
1
0.00980227
0.00980227
1.05
0.3639
Error
4
0.03742040
0.00935510
Corrected
Total
5
0.04722267
R­
Square
Coeff
Var
Root
MSE
WEIGHT
Mean
0.207575
16.18803
0.096722
0.597489
Source
DF
Anova
SS
Mean
Square
F
Value
Pr
>
F
Sex
1
0.00980227
0.00980227
1.05
0.3639
Dunnett's
t
Tests
for
WEIGHT
NOTE:
This
test
controls
the
Type
I
experimentwise
error
for
comparisons
of
all
treatments
against
a
control.

Alpha
0.05
Error
Degrees
of
Freedom
4
Error
Mean
Square
0.009355
Critical
Value
of
Dunnett's
t
2.77630
Minimum
Significant
Difference
0.2193
Comparisons
significant
at
the
0.05
level
are
indicated
by
***.

Difference
Sex
Between
Simultaneous
95%
Comparison
Means
Confidence
Limits
M
­
F
0.08084
­
0.13841
0.30009
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
67
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
718
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
0
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
8.0
10.50
2.291288
2.666667
M
3
13.0
10.50
2.291288
4.333333
Wilcoxon
Two­
Sample
Test
Statistic
8.0000
Normal
Approximation
Z
­
0.8729
One­
Sided
Pr
<
Z
0.1914
Two­
Sided
Pr
>
|
Z|
0.3827
t
Approximation
One­
Sided
Pr
<
Z
0.2113
Two­
Sided
Pr
>
|
Z|
0.4227
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
1.1905
DF
1
Pr
>
Chi­
Square
0.2752
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
1.0
1.50
0.670820
0.333333
M
3
2.0
1.50
0.670820
0.666667
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.7454
One­
Sided
Pr
<
Z
0.2280
Two­
Sided
Pr
>
|
Z|
0.4561
Median
One­
Way
Analysis
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
68
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
720
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
1
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
5.0
5.0
1.290994
2.50
M
2
5.0
5.0
1.290994
2.50
Wilcoxon
Two­
Sample
Test
Statistic
5.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
1.0
1.0
0.577350
0.50
M
2
1.0
1.0
0.577350
0.50
Median
Two­
Sample
Test
Statistic
1.0000
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Median
One­
Way
Analysis
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
69
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
722
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
10
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
5.0
5.0
1.290994
2.50
M
2
5.0
5.0
1.290994
2.50
Wilcoxon
Two­
Sample
Test
Statistic
5.0000
Normal
Approximation
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
t
Approximation
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
2
1.0
1.0
0.577350
0.50
M
2
1.0
1.0
0.577350
0.50
Median
Two­
Sample
Test
Statistic
1.0000
Z
0.0000
One­
Sided
Pr
<
Z
0.5000
Two­
Sided
Pr
>
|
Z|
1.0000
Median
One­
Way
Analysis
Chi­
Square
0.0000
DF
1
Pr
>
Chi­
Square
1.0000
Data
Evaluation
Report
on
Exposure
of
Xenopus
laevis
larvae
to
Different
Concentrations
of
Atrazine
in
Semi­
Natural
Microcosms
EPA
MRID
Number
458677­
11
Page
70
of
70
NONPARAMETRIC
COMPARISON
OF
FROG
LENGTH
BETWEEN
SEXES
FOR
EACH
EXPOSURE
GROUP
724
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­
ATRAZINE=
25
­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­­

The
NPAR1WAY
Procedure
Wilcoxon
Scores
(
Rank
Sums)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
9.0
10.50
2.291288
3.0
M
3
12.0
10.50
2.291288
4.0
Wilcoxon
Two­
Sample
Test
Statistic
9.0000
Normal
Approximation
Z
­
0.4364
One­
Sided
Pr
<
Z
0.3313
Two­
Sided
Pr
>
|
Z|
0.6625
t
Approximation
One­
Sided
Pr
<
Z
0.3404
Two­
Sided
Pr
>
|
Z|
0.6807
Z
includes
a
continuity
correction
of
0.5.

Kruskal­
Wallis
Test
Chi­
Square
0.4286
DF
1
Pr
>
Chi­
Square
0.5127
Median
Scores
(
Number
of
Points
Above
Median)
for
Variable
WEIGHT
Classified
by
Variable
Sex
Sum
of
Expected
Std
Dev
Mean
Sex
N
Scores
Under
H0
Under
H0
Score
âââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââââ
F
3
1.0
1.50
0.670820
0.333333
M
3
2.0
1.50
0.670820
0.666667
Median
Two­
Sample
Test
Statistic
1.0000
Z
­
0.7454
One­
Sided
Pr
<
Z
0.2280
Two­
Sided
Pr
>
|
Z|
0.4561
Median
One­
Way
Analysis
Chi­
Square
0.5556
DF
1
Pr
>
Chi­
Square
0.4561
